filipin dye  (Millipore)


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    Structured Review

    Millipore filipin dye
    Filipin Dye, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin dye/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    filipin dye - by Bioz Stars, 2020-04
    86/100 stars

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    Staining:

    Article Title: The Yeast P5 Type ATPase, Spf1, Regulates Manganese Transport into the Endoplasmic Reticulum
    Article Snippet: .. In case of the filipin staining, strains were grown overnight to stationary phase and then samples were stained for sterol, using the filipin dye (Sigma). .. Yeast Strain and Strain Construction All strains were constructed on the S288C (BY4741) genetic background [ ].

    Plasmid Preparation:

    Article Title: The Yeast P5 Type ATPase, Spf1, Regulates Manganese Transport into the Endoplasmic Reticulum
    Article Snippet: Yeast Media and Growth Conditions Yeast cells were grown at 30°C in either rich medium, YPD (1% Bacto-yeast extract (BD), 2% Bacto-peptone (BD) and 2% dextrose (Amresco)) or synthetic medium, SD (0.67% yeast nitrogen base with ammonium sulfate and without amino acids (CondaPronadisa) and 2% dextrose (Amresco), containing the appropriate supplements for plasmid selection) [ ]. .. In case of the filipin staining, strains were grown overnight to stationary phase and then samples were stained for sterol, using the filipin dye (Sigma).

    Selection:

    Article Title: The Yeast P5 Type ATPase, Spf1, Regulates Manganese Transport into the Endoplasmic Reticulum
    Article Snippet: When needed for selection, the media was supplemented with G418 (200 ug/ml, Calbiochem) or nourseothricin (Nat) (200 ug/ml WERNER BioAgents). .. In case of the filipin staining, strains were grown overnight to stationary phase and then samples were stained for sterol, using the filipin dye (Sigma).

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    Millipore filipin iii
    Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL <t>filipin</t> <t>III</t> for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p
    Filipin Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin iii/product/Millipore
    Average 99 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    filipin iii - by Bioz Stars, 2020-04
    99/100 stars
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    Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL filipin III for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Anandamide exerts its antiproliferative actions on cholangiocarcinoma by activation of the GPR55 receptor

    doi: 10.1038/labinvest.2011.62

    Figure Lengend Snippet: Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL filipin III for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p

    Article Snippet: The inhibitors utilized were the lipid raft disruptors, methyl-β-cyclodextrin (0.1 mM), , and filipin III (1 mg/mL) ( , ), and the JNK inhibitor (10−7 M; SP600125; EMD Chemicals, Gibbstown, NJ) ( ).

    Techniques: Activation Assay