filipin complex  (Millipore)


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    Structured Review

    Millipore filipin complex
    <t>Filipin</t> staining of various C. albicans strains. All strains were grown to exponential phase in YEPD at 30°C. Cells were then transferred to serum containing medium and grown at 37°C. After shift to serum, cells were fixed at the indicated
    Filipin Complex, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin complex/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    filipin complex - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Arv1 lipid transporter function is conserved between pathogenic and nonpathogenic fungi"

    Article Title: Arv1 lipid transporter function is conserved between pathogenic and nonpathogenic fungi

    Journal: Fungal Genetics and Biology

    doi: 10.1016/j.fgb.2011.11.006

    Filipin staining of various C. albicans strains. All strains were grown to exponential phase in YEPD at 30°C. Cells were then transferred to serum containing medium and grown at 37°C. After shift to serum, cells were fixed at the indicated
    Figure Legend Snippet: Filipin staining of various C. albicans strains. All strains were grown to exponential phase in YEPD at 30°C. Cells were then transferred to serum containing medium and grown at 37°C. After shift to serum, cells were fixed at the indicated

    Techniques Used: Staining

    2) Product Images from "A voltage-dependent K+ channel in the lysosome is required for refilling lysosomal Ca2+ stores"

    Article Title: A voltage-dependent K+ channel in the lysosome is required for refilling lysosomal Ca2+ stores

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201612123

    LysoK VCa is required for lysosome function. (A) Western blot analysis of Lamp1 expression in WT and KCNMA1 KO MEFs. (B) LysoTracker staining in WT and KCNMA1 KO MEFs. Bar, 10 µm. (C) Confocal imaging of DQ-red-BSA in starved Cos-1 cells (amino acid + serum starvation) in the presence of paxilline (10 µM), quinidine (500 µM), or IBTX (100 nM). Bar, 10 µm. (D) Normalized proteolytic index for starved Cos-1 cells treated with paxilline or quinidine. (E) Normalized proteolytic index for starved WT and KCNMA1 KO MEFs treated with paxilline. (F) Cholesterol levels, detected with filipin staining, in WT, KCNMA1 KO, and NPC1 KO MEFs. Bar, 50 µm. (G) Normalized filipin density in WT, KCNMA1 KO, and NPC1 KO MEFs. Means ± SEM are shown in A, D, E, and G. Statistical comparisons were made using variance analysis (ANOVA for D, E, and G). *, P
    Figure Legend Snippet: LysoK VCa is required for lysosome function. (A) Western blot analysis of Lamp1 expression in WT and KCNMA1 KO MEFs. (B) LysoTracker staining in WT and KCNMA1 KO MEFs. Bar, 10 µm. (C) Confocal imaging of DQ-red-BSA in starved Cos-1 cells (amino acid + serum starvation) in the presence of paxilline (10 µM), quinidine (500 µM), or IBTX (100 nM). Bar, 10 µm. (D) Normalized proteolytic index for starved Cos-1 cells treated with paxilline or quinidine. (E) Normalized proteolytic index for starved WT and KCNMA1 KO MEFs treated with paxilline. (F) Cholesterol levels, detected with filipin staining, in WT, KCNMA1 KO, and NPC1 KO MEFs. Bar, 50 µm. (G) Normalized filipin density in WT, KCNMA1 KO, and NPC1 KO MEFs. Means ± SEM are shown in A, D, E, and G. Statistical comparisons were made using variance analysis (ANOVA for D, E, and G). *, P

    Techniques Used: Western Blot, Expressing, Staining, Imaging

    3) Product Images from "Mutations in the Cholesterol Transporter Gene ABCA5 Are Associated with Excessive Hair Overgrowth"

    Article Title: Mutations in the Cholesterol Transporter Gene ABCA5 Are Associated with Excessive Hair Overgrowth

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1004333

    Homozygous loss-of-function of ABCA5 perturbs lysosome function, resulting in an overall accumulation of autophagosomes and intracellular cholesterol levels in CGHT keratinocytes. (A) Control and CGHT keratinocytes were cultured in normal media in the presence or absence of 50 nM bafilomycin, fixed and immunostained. Confocal analysis of LC3 (green), Lamp1 (red) and p62 (blue) revealed an increased number of LC3-positive particles as well strong accumulation of p62 particles in affected keratinocytes. BAF-treated control cells possessed a two-fold increase in the number of LC3 puncta, whereas no significant difference was observed in BAF-treated affected cells (quantified in Figure S5 ). (B) Control and CGHT keratinocytes were fixed and immunostained for Filipin and Lamp1 (red), which revealed an accumulation and redistribution of free cholesterol to Lamp1-positive organelles in affected keratinocytes.
    Figure Legend Snippet: Homozygous loss-of-function of ABCA5 perturbs lysosome function, resulting in an overall accumulation of autophagosomes and intracellular cholesterol levels in CGHT keratinocytes. (A) Control and CGHT keratinocytes were cultured in normal media in the presence or absence of 50 nM bafilomycin, fixed and immunostained. Confocal analysis of LC3 (green), Lamp1 (red) and p62 (blue) revealed an increased number of LC3-positive particles as well strong accumulation of p62 particles in affected keratinocytes. BAF-treated control cells possessed a two-fold increase in the number of LC3 puncta, whereas no significant difference was observed in BAF-treated affected cells (quantified in Figure S5 ). (B) Control and CGHT keratinocytes were fixed and immunostained for Filipin and Lamp1 (red), which revealed an accumulation and redistribution of free cholesterol to Lamp1-positive organelles in affected keratinocytes.

    Techniques Used: Cell Culture

    4) Product Images from "Cholesterol-Enriched Membrane Microdomains Are Required for Inducing Host Cell Cytoskeleton Rearrangements in Response to Attaching-Effacing Escherichia coli"

    Article Title: Cholesterol-Enriched Membrane Microdomains Are Required for Inducing Host Cell Cytoskeleton Rearrangements in Response to Attaching-Effacing Escherichia coli

    Journal:

    doi: 10.1128/IAI.73.11.7113-7125.2005

    Bacterially induced cytoskeleton mobilization events are caveola independent. (a and b) Immunofluorescence micrographs stained for α-actinin, EHEC, or EPEC and merged images. HEp-2 cells were treated with 1 mg/ml filipin for 1 h prior to infection
    Figure Legend Snippet: Bacterially induced cytoskeleton mobilization events are caveola independent. (a and b) Immunofluorescence micrographs stained for α-actinin, EHEC, or EPEC and merged images. HEp-2 cells were treated with 1 mg/ml filipin for 1 h prior to infection

    Techniques Used: Immunofluorescence, Staining, Infection

    5) Product Images from "Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells"

    Article Title: Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1520490112

    NPC1-deficient human fibroblasts harbor less cholesterol upon BADG treatment. ( A ) Immunoblot of LAMP1 protein after BADG treatment (48 h). ( B ) Filipin intensity over lysosomes quantified using CellProfiler. The results are expressed as an average of the
    Figure Legend Snippet: NPC1-deficient human fibroblasts harbor less cholesterol upon BADG treatment. ( A ) Immunoblot of LAMP1 protein after BADG treatment (48 h). ( B ) Filipin intensity over lysosomes quantified using CellProfiler. The results are expressed as an average of the

    Techniques Used:

    Related Articles

    Microscopy:

    Article Title: Multi-organ Abnormalities and mTORC1 Activation in Zebrafish Model of Multiple Acyl-CoA Dehydrogenase Deficiency
    Article Snippet: Filipin and Periodic Acid-Schiff (PAS) Staining For free cholesterol staining on transversely sectioned larvae, slides were soaked with 1× PBS for 5 minutes, then Filipin complex diluted 1∶500 (Sigma, F-976) was added directly to slides and stained for 1 minute in the dark. .. Images were taken using the DAPI channel of a fluorescent microscope.

    Article Title: Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly
    Article Snippet: Washed cells were incubated with 5 mg/ml filipin complex (Sigma Chemicals) in the dark for 15 min at 23°. .. Filipin-based fluorescence was observed by spotting 3 μl of the cell suspensions onto poly-lysine microscope slides under UV light microscope using DAPI filter [ ].

    Article Title: Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence
    Article Snippet: Paragraph title: Filipin staining and fluorescence microscopy ... Washed cells were resuspended in 1 ml of water; 200 µl was mixed with 4 µl of freshly made 5 mg/ml filipin complex in ethanol (Sigma-Aldrich, St. Louis, MO).

    Imaging:

    Article Title: PEG-lipid micelles enable cholesterol efflux in Niemann-Pick Type C1 disease-based lysosomal storage disorder
    Article Snippet: Paragraph title: Imaging ... Cells were washed in PBS and 2 mM Glycine in PBS for 10 minutes, prior to staining with 0.05 mg/mL, filipin complex (Sigma Aldrich) in 10% FBS for 2 hours.

    In Situ:

    Article Title: SRL-Coated PAMAM Dendrimer Nano-Carrier for Targeted Gene Delivery to the Glioma Cells and Competitive Inhibition by Lactoferrin
    Article Snippet: The PAMAM G4 dendrimer was synthesized by an in-situ branch cell method, a two-step iterative process to construct poly (amidoamine) (PAMAM) dendrimers possessing either terminal ester or amine groups. .. Lactoferrin, filipin complex (from Streptomyces filipinesis), L-polylysin (30000-70000MW) and phenylarsine oxide were bought from Sigma-Aldrich (St. Louis, MO, USA).

    Fluorescence:

    Article Title: Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly
    Article Snippet: Washed cells were incubated with 5 mg/ml filipin complex (Sigma Chemicals) in the dark for 15 min at 23°. .. Filipin-based fluorescence was observed by spotting 3 μl of the cell suspensions onto poly-lysine microscope slides under UV light microscope using DAPI filter [ ].

    Article Title: Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence
    Article Snippet: Paragraph title: Filipin staining and fluorescence microscopy ... Washed cells were resuspended in 1 ml of water; 200 µl was mixed with 4 µl of freshly made 5 mg/ml filipin complex in ethanol (Sigma-Aldrich, St. Louis, MO).

    Synthesized:

    Article Title: SRL-Coated PAMAM Dendrimer Nano-Carrier for Targeted Gene Delivery to the Glioma Cells and Competitive Inhibition by Lactoferrin
    Article Snippet: The PAMAM G4 dendrimer was synthesized by an in-situ branch cell method, a two-step iterative process to construct poly (amidoamine) (PAMAM) dendrimers possessing either terminal ester or amine groups. .. Lactoferrin, filipin complex (from Streptomyces filipinesis), L-polylysin (30000-70000MW) and phenylarsine oxide were bought from Sigma-Aldrich (St. Louis, MO, USA).

    Isolation:

    Article Title: Segregation of CD4 and CXCR4 into Distinct Lipid Microdomains in T Lymphocytes Suggests a Mechanism for Membrane Destabilization by Human Immunodeficiency Virus
    Article Snippet: To characterize the lipid raft isolation procedure and to determine the effects of ligand binding on CD4 and CXCR4 distributions, H9 cells were treated with various reagents prior to lysing. .. Cell membrane cholesterol was partially extracted by incubating cells in serum-free RPMI 1640 medium supplemented with 25 mM HEPES and filipin III complex (1 μg/ml) (Sigma Diagnostics Inc.) at 37°C for 1 h ( ) or with methyl-β-cyclodextrin (10 mM) (Sigma Diagnostics, Inc.) at 37°C, with occasional agitation, for 2 h ( , ).

    Construct:

    Article Title: SRL-Coated PAMAM Dendrimer Nano-Carrier for Targeted Gene Delivery to the Glioma Cells and Competitive Inhibition by Lactoferrin
    Article Snippet: The PAMAM G4 dendrimer was synthesized by an in-situ branch cell method, a two-step iterative process to construct poly (amidoamine) (PAMAM) dendrimers possessing either terminal ester or amine groups. .. Lactoferrin, filipin complex (from Streptomyces filipinesis), L-polylysin (30000-70000MW) and phenylarsine oxide were bought from Sigma-Aldrich (St. Louis, MO, USA).

    Purification:

    Article Title: SRL-Coated PAMAM Dendrimer Nano-Carrier for Targeted Gene Delivery to the Glioma Cells and Competitive Inhibition by Lactoferrin
    Article Snippet: Materials and Methods The pEGFP-N1 plasmid (Clontech, Palo Alto, CA, USA) was purified using a QIAGEN Plasmid Mega Kit (Qiagen GmbH, Hildden, Germany). .. Lactoferrin, filipin complex (from Streptomyces filipinesis), L-polylysin (30000-70000MW) and phenylarsine oxide were bought from Sigma-Aldrich (St. Louis, MO, USA).

    Light Microscopy:

    Article Title: Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly
    Article Snippet: Washed cells were incubated with 5 mg/ml filipin complex (Sigma Chemicals) in the dark for 15 min at 23°. .. Filipin-based fluorescence was observed by spotting 3 μl of the cell suspensions onto poly-lysine microscope slides under UV light microscope using DAPI filter [ ].

    Concentration Assay:

    Article Title: Interaction of two antitumor peptides with membrane lipids – Influence of phosphatidylserine and cholesterol on specificity for melanoma cells
    Article Snippet: Filipin complex (Sigma-Aldrich Co. LLC) (excitation wavelength 352 nm; emission wavelength 454 nm) was used for cholesterol staining of cell membranes of NHDF and A375. .. Filipin was solved in DMSO at a concentration of 25 mg/ml.

    Incubation:

    Article Title: Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly
    Article Snippet: .. Washed cells were incubated with 5 mg/ml filipin complex (Sigma Chemicals) in the dark for 15 min at 23°. .. Filipin-based fluorescence was observed by spotting 3 μl of the cell suspensions onto poly-lysine microscope slides under UV light microscope using DAPI filter [ ].

    Article Title: PEG-lipid micelles enable cholesterol efflux in Niemann-Pick Type C1 disease-based lysosomal storage disorder
    Article Snippet: Cells were washed in PBS and 2 mM Glycine in PBS for 10 minutes, prior to staining with 0.05 mg/mL, filipin complex (Sigma Aldrich) in 10% FBS for 2 hours. .. After a 24-hour incubation, media was removed, cells were washed in PBS and fixed in 4% paraformaldehyde at room temperature for 20 minutes, followed by filipin staining.

    Article Title: Segregation of CD4 and CXCR4 into Distinct Lipid Microdomains in T Lymphocytes Suggests a Mechanism for Membrane Destabilization by Human Immunodeficiency Virus
    Article Snippet: To activate protein kinase C, H9 cells were washed in PBS, incubated in warm serum-free RPMI 1640 medium supplemented with 25 mM HEPES and phorbol 12-myristate 13-acetate (PMA) (100 ng/ml) (Sigma Diagnostics, Inc.) at 37°C for 30 min ( ). .. Cell membrane cholesterol was partially extracted by incubating cells in serum-free RPMI 1640 medium supplemented with 25 mM HEPES and filipin III complex (1 μg/ml) (Sigma Diagnostics Inc.) at 37°C for 1 h ( ) or with methyl-β-cyclodextrin (10 mM) (Sigma Diagnostics, Inc.) at 37°C, with occasional agitation, for 2 h ( , ).

    other:

    Article Title: Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol
    Article Snippet: Lipoprotein-deficient serum was purchased from Biomedical Technologies Inc. Cholesterol, avasimibe, and filipin complex were purchased from Sigma-Aldrich.

    Article Title: Filipin-dependent Inhibition of Cholera Toxin: Evidence for Toxin Internalization and Activation through Caveolae-like Domains
    Article Snippet: Other reagents were obtained as follows: filipin complex, chlorpromazine, and 3-isobutyl-1-methylxanthine (IBMX) from Sigma ; brefeldin A (BFA) from Epicentre Technologies (Madison, WI); Na125 I (carrier-free) and 125 I-protein A (9.5 μCi/μg) from Dupont-New England Nuclear (Boston, MA).

    Article Title: Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells
    Article Snippet: Filipin complex, Nystatin, sucrose, phorbol dibutyrate (PdBu) and histamine were purchased from Sigma (Poole, Dorset, U.K.).

    Cell Culture:

    Article Title: SRL-Coated PAMAM Dendrimer Nano-Carrier for Targeted Gene Delivery to the Glioma Cells and Competitive Inhibition by Lactoferrin
    Article Snippet: Lactoferrin, filipin complex (from Streptomyces filipinesis), L-polylysin (30000-70000MW) and phenylarsine oxide were bought from Sigma-Aldrich (St. Louis, MO, USA). .. Cell line Rat Brain capillary endothelial cells (BCECs) were kindly provided from Dr. Aghaei (Neuroscience Research Center, Shahid Beheshti University of Medical Sciences), and cultured according to ATCC guidelines.

    Article Title: PEG-lipid micelles enable cholesterol efflux in Niemann-Pick Type C1 disease-based lysosomal storage disorder
    Article Snippet: Imaging Npc1 −/− and Npc1 +/+ immortalized mouse embryonic fibroblasts (MEFs) (kind gifts from the Langer Lab at MIT) were cultured in DMEM/10% FBS at 37 °C. .. Cells were washed in PBS and 2 mM Glycine in PBS for 10 minutes, prior to staining with 0.05 mg/mL, filipin complex (Sigma Aldrich) in 10% FBS for 2 hours.

    Article Title: Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence
    Article Snippet: One milliliter of 37.5% formaldehyde was added to 9 ml of cell culture grown to a density of 0.7 OD600 U/ml. .. Washed cells were resuspended in 1 ml of water; 200 µl was mixed with 4 µl of freshly made 5 mg/ml filipin complex in ethanol (Sigma-Aldrich, St. Louis, MO).

    Protease Inhibitor:

    Article Title: Lipid Raft-dependent Glucagon-like Peptide-2 Receptor Trafficking Occurs Independently of Agonist-induced Desensitization
    Article Snippet: .. Forskolin, 3-isobutyl-1-methylxanthine (IBMX), forskolin, anti-FLAG (M1) antibody, anti-mouse IgG-agarose, mouse IgG, 2,2′-azino-bis(3-ethylbenthiazoline-6-sulfonic acid) reagent, protease inhibitor cocktail, phosphatase inhibitor cocktail I, filipin complex, methyl-β-cyclodextrin (MβCD), and sodium monensin were all obtained from Sigma-Aldrich (St. Louis, MO). .. Peptide N -glycanase F (PNGase F) and restriction enzymes were from New England Biolabs (Mississauga, Ontario, Canada).

    Western Blot:

    Article Title: Segregation of CD4 and CXCR4 into Distinct Lipid Microdomains in T Lymphocytes Suggests a Mechanism for Membrane Destabilization by Human Immunodeficiency Virus
    Article Snippet: Collected samples were analyzed by Western immunoblotting (see below). .. Cell membrane cholesterol was partially extracted by incubating cells in serum-free RPMI 1640 medium supplemented with 25 mM HEPES and filipin III complex (1 μg/ml) (Sigma Diagnostics Inc.) at 37°C for 1 h ( ) or with methyl-β-cyclodextrin (10 mM) (Sigma Diagnostics, Inc.) at 37°C, with occasional agitation, for 2 h ( , ).

    Transformation Assay:

    Article Title: Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly
    Article Snippet: Control untransformed and transformed yeasts were grown in SC minimal media supplemented with 2% galactose and 50 μM CuSO4 for 24 hours at 23°C, 27°C and 32°C. .. Washed cells were incubated with 5 mg/ml filipin complex (Sigma Chemicals) in the dark for 15 min at 23°.

    Labeling:

    Article Title: Caveolin-1 mediated uptake via langerin restricts HIV-1 infection in human Langerhans cells
    Article Snippet: .. Antibodies and reagents The following antibodies were used: Rabbit-anti-Caveolin-1 (Cell Signalling); Goat-anti-langerin (R & D); DCGM4-PE (mouse-anti-langerin; Beckman Coulter); KC57-RD1-PE (mouse-anti-p24; Beckman Coulter); mouse-anti-CD1a-FITC (BD Pharmingen); anti-LAMP2 (Abcam); anti-CD63 (BD); sheep-anti-p24 (Aalto); 10E2 (anti-langerin [ ]); 10E2 coupled to Alexa-647 (Alexa-647 labeling kit); Streptavidin-Alexa-488; Goat-anti-Mouse IgG1 Alexa 546; Goat-anti-Rabbit Alexa 488 (5 μg/ml, all Invitrogen); 15 nm protein A-gold; 10 nm protein G-gold (both Aurion); prot A/G plus agarose beads (Santa Cruz); filipin complex (Sigma Aldrich); Dispase II (Roche Diagnostics); monodansylcadaverine (Sigma Aldrich). ..

    Ligand Binding Assay:

    Article Title: Segregation of CD4 and CXCR4 into Distinct Lipid Microdomains in T Lymphocytes Suggests a Mechanism for Membrane Destabilization by Human Immunodeficiency Virus
    Article Snippet: To characterize the lipid raft isolation procedure and to determine the effects of ligand binding on CD4 and CXCR4 distributions, H9 cells were treated with various reagents prior to lysing. .. Cell membrane cholesterol was partially extracted by incubating cells in serum-free RPMI 1640 medium supplemented with 25 mM HEPES and filipin III complex (1 μg/ml) (Sigma Diagnostics Inc.) at 37°C for 1 h ( ) or with methyl-β-cyclodextrin (10 mM) (Sigma Diagnostics, Inc.) at 37°C, with occasional agitation, for 2 h ( , ).

    Plasmid Preparation:

    Article Title: SRL-Coated PAMAM Dendrimer Nano-Carrier for Targeted Gene Delivery to the Glioma Cells and Competitive Inhibition by Lactoferrin
    Article Snippet: Materials and Methods The pEGFP-N1 plasmid (Clontech, Palo Alto, CA, USA) was purified using a QIAGEN Plasmid Mega Kit (Qiagen GmbH, Hildden, Germany). .. Lactoferrin, filipin complex (from Streptomyces filipinesis), L-polylysin (30000-70000MW) and phenylarsine oxide were bought from Sigma-Aldrich (St. Louis, MO, USA).

    Staining:

    Article Title: Multi-organ Abnormalities and mTORC1 Activation in Zebrafish Model of Multiple Acyl-CoA Dehydrogenase Deficiency
    Article Snippet: .. Filipin and Periodic Acid-Schiff (PAS) Staining For free cholesterol staining on transversely sectioned larvae, slides were soaked with 1× PBS for 5 minutes, then Filipin complex diluted 1∶500 (Sigma, F-976) was added directly to slides and stained for 1 minute in the dark. ..

    Article Title: Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly
    Article Snippet: Paragraph title: Filipin-based staining of ergoterols in yeasts ... Washed cells were incubated with 5 mg/ml filipin complex (Sigma Chemicals) in the dark for 15 min at 23°.

    Article Title: PEG-lipid micelles enable cholesterol efflux in Niemann-Pick Type C1 disease-based lysosomal storage disorder
    Article Snippet: .. Cells were washed in PBS and 2 mM Glycine in PBS for 10 minutes, prior to staining with 0.05 mg/mL, filipin complex (Sigma Aldrich) in 10% FBS for 2 hours. .. Cells were then washed in PBS three times and stored in 100 μL PBS with 1x HCS NuclearMask Deep Red Stain (Life Technologies) at 4 °C for further analysis.

    Article Title: Interaction of two antitumor peptides with membrane lipids – Influence of phosphatidylserine and cholesterol on specificity for melanoma cells
    Article Snippet: .. Filipin complex (Sigma-Aldrich Co. LLC) (excitation wavelength 352 nm; emission wavelength 454 nm) was used for cholesterol staining of cell membranes of NHDF and A375. .. Filipin was solved in DMSO at a concentration of 25 mg/ml.

    Article Title: Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence
    Article Snippet: Paragraph title: Filipin staining and fluorescence microscopy ... Washed cells were resuspended in 1 ml of water; 200 µl was mixed with 4 µl of freshly made 5 mg/ml filipin complex in ethanol (Sigma-Aldrich, St. Louis, MO).

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    Millipore filipin iii
    Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL <t>filipin</t> <t>III</t> for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p
    Filipin Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin iii/product/Millipore
    Average 99 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    filipin iii - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore filipin complex
    Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL <t>filipin</t> <t>III</t> for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p
    Filipin Complex, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin complex/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    filipin complex - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL filipin III for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Anandamide exerts its antiproliferative actions on cholangiocarcinoma by activation of the GPR55 receptor

    doi: 10.1038/labinvest.2011.62

    Figure Lengend Snippet: Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL filipin III for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p

    Article Snippet: The inhibitors utilized were the lipid raft disruptors, methyl-β-cyclodextrin (0.1 mM), , and filipin III (1 mg/mL) ( , ), and the JNK inhibitor (10−7 M; SP600125; EMD Chemicals, Gibbstown, NJ) ( ).

    Techniques: Activation Assay