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Greiner Bio ficoll paque
Ficoll Paque, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Greiner Bio
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Isolation:

Article Title: Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation
Article Snippet: .. Human PBMCs were isolated from blood collected in EDTA-coated Vacutainer tubes (BD) followed by Ficoll-Paque (Leucosep tubes; Greiner Bio-One) density gradient centrifugation. .. After isolation, PBMCs were stored in liquid nitrogen for later use.

Article Title: The Effect of C. burnetii Infection on the Cytokine Response of PBMCs from Pregnant Goats
Article Snippet: .. Isolation and stimulation of peripheral blood mononuclear cells The PBMC fraction was obtained by density centrifugation of EDTA blood using a Ficoll-Paque gradient (Pharmacia Biotech, USA) or Leucosep tubes prefilled with Ficoll-Paque (Greiner, the Netherlands). .. After washing, the cells were re-suspended in RPMI 1640 Dutch modified (Gibco Invitrogen, USA) supplemented with 50 mg/L gentamycin (Centrafarm, the Netherlands), 2 mM L-glutamine, and 1 mM pyruvate (Gibco Invitrogen, USA).

Article Title: TRPV4 inhibition attenuates stretch-induced inflammatory cellular responses and lung barrier dysfunction during mechanical ventilation
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by means of density gradient centrifugation using Ficoll-Paque™ and a Leucosep Tube (Greiner Bio-One GmbH) according to manufacturer’s instructions. .. CD14 positive monocyte purification was performed by magnetic activated cell sorting (MACS) according to the manufacturer’s instructions (Monocyte Isolation Kit II, Miltenyi Biotec) and seeded 2x106 cells/cm2 in XVIVO-10 medium (Lonza) on Collagen Type I-coated BioFlex® plates.

Article Title: Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing
Article Snippet: .. Human monocyte derived macrophages Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human whole blood by means of density gradient centrifugation using Ficoll-Paque™ and a Leucosep Tube (Greiner Bio-One GmbH) according to manufacturer’s instructions. .. Blood was donated by internal donors at the centre for occupational health at Boehringer Ingelheim in Biberach, the donors provided signed informed consent that allows use for scientific purposes.

Article Title: The pharmacokinetics and pharmacodynamics of mycophenolate mofetil in younger and elderly renal transplant recipients) The pharmacokinetics and pharmacodynamics of mycophenolate mofetil in younger and elderly renal transplant recipients
Article Snippet: .. PBMCs were isolated with Ficoll‐Paque (Greiner Bio‐One, Alphen a/d Rijn, The Netherlands) according to the manufacturer's protocol. ..

Centrifugation:

Article Title: The Effect of C. burnetii Infection on the Cytokine Response of PBMCs from Pregnant Goats
Article Snippet: .. Isolation and stimulation of peripheral blood mononuclear cells The PBMC fraction was obtained by density centrifugation of EDTA blood using a Ficoll-Paque gradient (Pharmacia Biotech, USA) or Leucosep tubes prefilled with Ficoll-Paque (Greiner, the Netherlands). .. After washing, the cells were re-suspended in RPMI 1640 Dutch modified (Gibco Invitrogen, USA) supplemented with 50 mg/L gentamycin (Centrafarm, the Netherlands), 2 mM L-glutamine, and 1 mM pyruvate (Gibco Invitrogen, USA).

Gradient Centrifugation:

Article Title: Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation
Article Snippet: .. Human PBMCs were isolated from blood collected in EDTA-coated Vacutainer tubes (BD) followed by Ficoll-Paque (Leucosep tubes; Greiner Bio-One) density gradient centrifugation. .. After isolation, PBMCs were stored in liquid nitrogen for later use.

Article Title: TRPV4 inhibition attenuates stretch-induced inflammatory cellular responses and lung barrier dysfunction during mechanical ventilation
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by means of density gradient centrifugation using Ficoll-Paque™ and a Leucosep Tube (Greiner Bio-One GmbH) according to manufacturer’s instructions. .. CD14 positive monocyte purification was performed by magnetic activated cell sorting (MACS) according to the manufacturer’s instructions (Monocyte Isolation Kit II, Miltenyi Biotec) and seeded 2x106 cells/cm2 in XVIVO-10 medium (Lonza) on Collagen Type I-coated BioFlex® plates.

Article Title: Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing
Article Snippet: .. Human monocyte derived macrophages Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human whole blood by means of density gradient centrifugation using Ficoll-Paque™ and a Leucosep Tube (Greiner Bio-One GmbH) according to manufacturer’s instructions. .. Blood was donated by internal donors at the centre for occupational health at Boehringer Ingelheim in Biberach, the donors provided signed informed consent that allows use for scientific purposes.

Derivative Assay:

Article Title: Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing
Article Snippet: .. Human monocyte derived macrophages Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human whole blood by means of density gradient centrifugation using Ficoll-Paque™ and a Leucosep Tube (Greiner Bio-One GmbH) according to manufacturer’s instructions. .. Blood was donated by internal donors at the centre for occupational health at Boehringer Ingelheim in Biberach, the donors provided signed informed consent that allows use for scientific purposes.

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    Greiner Bio standard ficoll paque gradient centrifugation
    Mean recovery (A) and viability (B) of paired fresh PBMCs isolated from 20 Tanzanian blood donors obtained using heparinized blood and standard <t>Ficoll-Paque</t> gradient separation or CPTs containing heparin as anticoagulant. Mean recovery (C) and viability (D) of fresh PBMCs isolated from 25 Tanzanian blood donors obtained using heparinized blood and standard Ficoll-Paque gradient separation or LeucoSep tubes.
    Standard Ficoll Paque Gradient Centrifugation, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard ficoll paque gradient centrifugation/product/Greiner Bio
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    standard ficoll paque gradient centrifugation - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    Greiner Bio ficoll paque
    Mean recovery (A) and viability (B) of paired fresh PBMCs isolated from 20 Tanzanian blood donors obtained using heparinized blood and standard <t>Ficoll-Paque</t> gradient separation or CPTs containing heparin as anticoagulant. Mean recovery (C) and viability (D) of fresh PBMCs isolated from 25 Tanzanian blood donors obtained using heparinized blood and standard Ficoll-Paque gradient separation or LeucoSep tubes.
    Ficoll Paque, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque/product/Greiner Bio
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ficoll paque - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Mean recovery (A) and viability (B) of paired fresh PBMCs isolated from 20 Tanzanian blood donors obtained using heparinized blood and standard Ficoll-Paque gradient separation or CPTs containing heparin as anticoagulant. Mean recovery (C) and viability (D) of fresh PBMCs isolated from 25 Tanzanian blood donors obtained using heparinized blood and standard Ficoll-Paque gradient separation or LeucoSep tubes.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Optimal Blood Mononuclear Cell Isolation Procedures for Gamma Interferon Enzyme-Linked Immunospot Testing of Healthy Swedish and Tanzanian Subjects

    doi: 10.1128/CVI.00161-07

    Figure Lengend Snippet: Mean recovery (A) and viability (B) of paired fresh PBMCs isolated from 20 Tanzanian blood donors obtained using heparinized blood and standard Ficoll-Paque gradient separation or CPTs containing heparin as anticoagulant. Mean recovery (C) and viability (D) of fresh PBMCs isolated from 25 Tanzanian blood donors obtained using heparinized blood and standard Ficoll-Paque gradient separation or LeucoSep tubes.

    Article Snippet: Standard Ficoll-Paque gradient centrifugation, BD Vacutainer cell preparation tube (CPT), and Greiner Bio-One LeucoSep tube techniques were tested.

    Techniques: Isolation

    Mean recovery (A) and viability (B) of paired fresh PBMCs isolated from Swedish blood donors obtained using heparinized blood and standard Ficoll-Paque gradient separation or CPTs containing heparin as an anticoagulant.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Optimal Blood Mononuclear Cell Isolation Procedures for Gamma Interferon Enzyme-Linked Immunospot Testing of Healthy Swedish and Tanzanian Subjects

    doi: 10.1128/CVI.00161-07

    Figure Lengend Snippet: Mean recovery (A) and viability (B) of paired fresh PBMCs isolated from Swedish blood donors obtained using heparinized blood and standard Ficoll-Paque gradient separation or CPTs containing heparin as an anticoagulant.

    Article Snippet: Standard Ficoll-Paque gradient centrifugation, BD Vacutainer cell preparation tube (CPT), and Greiner Bio-One LeucoSep tube techniques were tested.

    Techniques: Isolation