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Axis-Shield Diagnostics ficoll paque
Ficoll Paque, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ficoll paque/product/Axis-Shield Diagnostics
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
ficoll paque - by Bioz Stars, 2020-07
92/100 stars

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Isolation:

Article Title: Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus
Article Snippet: .. B-cell isolation, EBV infection and mitogen stimulation Blood lymphocytes were purified from Buffy coats (Blood Bank, Karolinska Hospital, Stockholm, Sweden) by Ficoll–Paque (Lymphoprep, Axis-shield PoC AS, Oslo, Norway) density gradient centrifugation and B cells were affinity purified using CD19 micro beads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) according to standard procedures. .. For EBV infection, the cells were pre-incubated for 1 h with spent supernatant from the EBV producer cell line B95.8, and then cultured in RPMI-1640, supplemented with 10–20% fetal bovine serum, 2 mM L -glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin, (all from Sigma-Aldrich, St Louis, MO, USA).

Article Title: Comparative study of clinical grade human tolerogenic dendritic cells
Article Snippet: .. Peripheral Blood Mononuclear Cells (PBMCs) were isolated by Ficoll-Paque (Lymphoprep, Axis Shield, Oslo, Norway) density gradient centrifugation at 400 × g for 25 min. .. Recovered cells were washed twice in PBS and counted using Perfect Count microspheres (Cytognos SL, Salamanca, Spain) following the manufacturer's instructions.

Article Title: Human umbilical cord derived mesenchymal stem cells promote interleukin‐17 production from human peripheral blood mononuclear cells of healthy donors and systemic lupus erythematosus patients
Article Snippet: .. Human peripheral blood mononuclear cells (hPBMCs) were isolated by Ficoll‐Paque (Axis‐Shield, Dundee, UK) density gradient centrifugation (density 1·077 ± 0·002 at 2200 rpm/min × 20 min) from the venous blood of healthy volunteers and SLE patients. .. A subpopulation of CD4+ T cells was purified by using relevant magnetic MicroBead kits (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

Article Title: Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood of consented healthy volunteers using Ficoll-Paque (Axis-Shield). .. Monocytes ( > 95% CD14+ ) were purified from PBMC using anti-CD14 microbeads (Miltenyi).

Article Title: Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors
Article Snippet: .. Preparation of Human CD4+ T Cells Human mononuclear cells from patients with ITP were isolated by Ficoll-Paque (Axis-Shield). .. Then, CD4+ T cells were obtained with magnetic MicroBead kits (Miltenyi Biotec).

Article Title: Human umbilical cord mesenchymal stem cells increase interleukin-9 production of CD4+ T cells
Article Snippet: .. Isolation of cord blood CD4+ T cells Human cord blood mononuclear cells were isolated by Ficoll-Paque (Axis-Shield, Oslo, Norway) density gradient centrifugation from blood of health volunteer donors. .. CD4+ T cells were purified by using relevant magnetic MicroBead kits (Miltenyi Biotec GmbH, Bergisch Gladbach, German) according to the manufacturer's instructions.

Infection:

Article Title: Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus
Article Snippet: .. B-cell isolation, EBV infection and mitogen stimulation Blood lymphocytes were purified from Buffy coats (Blood Bank, Karolinska Hospital, Stockholm, Sweden) by Ficoll–Paque (Lymphoprep, Axis-shield PoC AS, Oslo, Norway) density gradient centrifugation and B cells were affinity purified using CD19 micro beads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) according to standard procedures. .. For EBV infection, the cells were pre-incubated for 1 h with spent supernatant from the EBV producer cell line B95.8, and then cultured in RPMI-1640, supplemented with 10–20% fetal bovine serum, 2 mM L -glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin, (all from Sigma-Aldrich, St Louis, MO, USA).

Purification:

Article Title: Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus
Article Snippet: .. B-cell isolation, EBV infection and mitogen stimulation Blood lymphocytes were purified from Buffy coats (Blood Bank, Karolinska Hospital, Stockholm, Sweden) by Ficoll–Paque (Lymphoprep, Axis-shield PoC AS, Oslo, Norway) density gradient centrifugation and B cells were affinity purified using CD19 micro beads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) according to standard procedures. .. For EBV infection, the cells were pre-incubated for 1 h with spent supernatant from the EBV producer cell line B95.8, and then cultured in RPMI-1640, supplemented with 10–20% fetal bovine serum, 2 mM L -glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin, (all from Sigma-Aldrich, St Louis, MO, USA).

Affinity Purification:

Article Title: Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus
Article Snippet: .. B-cell isolation, EBV infection and mitogen stimulation Blood lymphocytes were purified from Buffy coats (Blood Bank, Karolinska Hospital, Stockholm, Sweden) by Ficoll–Paque (Lymphoprep, Axis-shield PoC AS, Oslo, Norway) density gradient centrifugation and B cells were affinity purified using CD19 micro beads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) according to standard procedures. .. For EBV infection, the cells were pre-incubated for 1 h with spent supernatant from the EBV producer cell line B95.8, and then cultured in RPMI-1640, supplemented with 10–20% fetal bovine serum, 2 mM L -glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin, (all from Sigma-Aldrich, St Louis, MO, USA).

Gradient Centrifugation:

Article Title: Evaluation of Costimulatory Molecules in Peripheral Blood Lymphocytes of Canine Patients with Histiocytic Sarcoma
Article Snippet: .. All heparinized blood samples were diluted with an equal volume of 0.9% saline, and PBLs were separated by Ficoll-Paque (Lymphoprep; Axis-Shield PoC AS, Oslo, Norway) density gradient centrifugation [ ]. .. Following centrifugation at 800 × g for 20 min at room temperature, PBLs were collected and washed twice with 0.9% saline.

Article Title: Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus
Article Snippet: .. B-cell isolation, EBV infection and mitogen stimulation Blood lymphocytes were purified from Buffy coats (Blood Bank, Karolinska Hospital, Stockholm, Sweden) by Ficoll–Paque (Lymphoprep, Axis-shield PoC AS, Oslo, Norway) density gradient centrifugation and B cells were affinity purified using CD19 micro beads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) according to standard procedures. .. For EBV infection, the cells were pre-incubated for 1 h with spent supernatant from the EBV producer cell line B95.8, and then cultured in RPMI-1640, supplemented with 10–20% fetal bovine serum, 2 mM L -glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin, (all from Sigma-Aldrich, St Louis, MO, USA).

Article Title: Comparative study of clinical grade human tolerogenic dendritic cells
Article Snippet: .. Peripheral Blood Mononuclear Cells (PBMCs) were isolated by Ficoll-Paque (Lymphoprep, Axis Shield, Oslo, Norway) density gradient centrifugation at 400 × g for 25 min. .. Recovered cells were washed twice in PBS and counted using Perfect Count microspheres (Cytognos SL, Salamanca, Spain) following the manufacturer's instructions.

Article Title: Human umbilical cord derived mesenchymal stem cells promote interleukin‐17 production from human peripheral blood mononuclear cells of healthy donors and systemic lupus erythematosus patients
Article Snippet: .. Human peripheral blood mononuclear cells (hPBMCs) were isolated by Ficoll‐Paque (Axis‐Shield, Dundee, UK) density gradient centrifugation (density 1·077 ± 0·002 at 2200 rpm/min × 20 min) from the venous blood of healthy volunteers and SLE patients. .. A subpopulation of CD4+ T cells was purified by using relevant magnetic MicroBead kits (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

Article Title: Human umbilical cord mesenchymal stem cells increase interleukin-9 production of CD4+ T cells
Article Snippet: .. Isolation of cord blood CD4+ T cells Human cord blood mononuclear cells were isolated by Ficoll-Paque (Axis-Shield, Oslo, Norway) density gradient centrifugation from blood of health volunteer donors. .. CD4+ T cells were purified by using relevant magnetic MicroBead kits (Miltenyi Biotec GmbH, Bergisch Gladbach, German) according to the manufacturer's instructions.

Magnetic Cell Separation:

Article Title: Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus
Article Snippet: .. B-cell isolation, EBV infection and mitogen stimulation Blood lymphocytes were purified from Buffy coats (Blood Bank, Karolinska Hospital, Stockholm, Sweden) by Ficoll–Paque (Lymphoprep, Axis-shield PoC AS, Oslo, Norway) density gradient centrifugation and B cells were affinity purified using CD19 micro beads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) according to standard procedures. .. For EBV infection, the cells were pre-incubated for 1 h with spent supernatant from the EBV producer cell line B95.8, and then cultured in RPMI-1640, supplemented with 10–20% fetal bovine serum, 2 mM L -glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin, (all from Sigma-Aldrich, St Louis, MO, USA).

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    Axis-Shield Diagnostics ficoll paque plus
    Ficoll Paque Plus, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ficoll paque plus/product/Axis-Shield Diagnostics
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    ficoll paque plus - by Bioz Stars, 2020-07
    92/100 stars
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