fibronectin coated dishes  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    AarI AjiI Bpu10I ScaI PasI Buffer 10X
    Description:
    Thermo Scientific 10X Buffer AarI AjiI Bpu10I ScaI PasI is the optimal buffer recommended for use with AarI AjiI Bpu10I ScaI and PasI restriction enzymes and is premixed with BSA for enhanced stability To ensure consistent enzyme performance Thermo Scientific restriction enzyme buffers contain BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Multiple freeze thaw cycles of the buffers will not cause BSA precipitation Thermo Scientific restriction enzymes exhibit 100 of their certified activity in the recommended buffer
    Catalog Number:
    B27
    Price:
    None
    Category:
    Lab Reagents and Chemicals
    Applications:
    Cloning|Restriction Enzyme Cloning
    Buy from Supplier


    Structured Review

    Thermo Fisher fibronectin coated dishes
    Specific induction of FAK phosphorylation by α5β1-mediated adhesion to <t>fibronectin.</t> Cells were serum starved overnight, trypsinized, neutralized with soybean trypsin inhibitor, and plated on fibronectin-coated plates at densities of 0, 50, 100, 150, and 350 ng/cm 2 for 60 min at room temperature (without phosphatase inhibitors). (A) Normal CEF extracts were analyzed by for phosphorylation of FAK(Y397), FAK(Y407), FAK(Y577), FAK(Y861), FAK(Y925), and total FAK protein. (B) Quantification of relative phosphorylation of Y397 in CEF; phosphorylated pY397/total FAK for each point. (C) Similar quantification of relative phosphorylation of Y861. (D) v-src-transformed CEF extracts were analyzed for specific phosphorylation of FAK(Y397), FAK(Y407), FAK(Y577), FAK(Y861), FAK(Y925), and total FAK. (E) Quantification of relative phosphorylation of Y397, Y861, and Y925 normalized for total FAK in each extract. (F) Similar quantification of Y407 and Y577. Error bars indicate the standard deviations ( n = 3). Symbols: ▪, pY397; ▾, pY407; ▪, pY577; ○, pY861; ▾, pY925.
    Thermo Scientific 10X Buffer AarI AjiI Bpu10I ScaI PasI is the optimal buffer recommended for use with AarI AjiI Bpu10I ScaI and PasI restriction enzymes and is premixed with BSA for enhanced stability To ensure consistent enzyme performance Thermo Scientific restriction enzyme buffers contain BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Multiple freeze thaw cycles of the buffers will not cause BSA precipitation Thermo Scientific restriction enzymes exhibit 100 of their certified activity in the recommended buffer
    https://www.bioz.com/result/fibronectin coated dishes/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fibronectin coated dishes - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "Transformation of Chicken Embryo Fibroblasts by v-src Uncouples ?1 Integrin-Mediated Outside-in but Not Inside-out Signaling"

    Article Title: Transformation of Chicken Embryo Fibroblasts by v-src Uncouples ?1 Integrin-Mediated Outside-in but Not Inside-out Signaling

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.21.21.7295-7306.2001

    Specific induction of FAK phosphorylation by α5β1-mediated adhesion to fibronectin. Cells were serum starved overnight, trypsinized, neutralized with soybean trypsin inhibitor, and plated on fibronectin-coated plates at densities of 0, 50, 100, 150, and 350 ng/cm 2 for 60 min at room temperature (without phosphatase inhibitors). (A) Normal CEF extracts were analyzed by for phosphorylation of FAK(Y397), FAK(Y407), FAK(Y577), FAK(Y861), FAK(Y925), and total FAK protein. (B) Quantification of relative phosphorylation of Y397 in CEF; phosphorylated pY397/total FAK for each point. (C) Similar quantification of relative phosphorylation of Y861. (D) v-src-transformed CEF extracts were analyzed for specific phosphorylation of FAK(Y397), FAK(Y407), FAK(Y577), FAK(Y861), FAK(Y925), and total FAK. (E) Quantification of relative phosphorylation of Y397, Y861, and Y925 normalized for total FAK in each extract. (F) Similar quantification of Y407 and Y577. Error bars indicate the standard deviations ( n = 3). Symbols: ▪, pY397; ▾, pY407; ▪, pY577; ○, pY861; ▾, pY925.
    Figure Legend Snippet: Specific induction of FAK phosphorylation by α5β1-mediated adhesion to fibronectin. Cells were serum starved overnight, trypsinized, neutralized with soybean trypsin inhibitor, and plated on fibronectin-coated plates at densities of 0, 50, 100, 150, and 350 ng/cm 2 for 60 min at room temperature (without phosphatase inhibitors). (A) Normal CEF extracts were analyzed by for phosphorylation of FAK(Y397), FAK(Y407), FAK(Y577), FAK(Y861), FAK(Y925), and total FAK protein. (B) Quantification of relative phosphorylation of Y397 in CEF; phosphorylated pY397/total FAK for each point. (C) Similar quantification of relative phosphorylation of Y861. (D) v-src-transformed CEF extracts were analyzed for specific phosphorylation of FAK(Y397), FAK(Y407), FAK(Y577), FAK(Y861), FAK(Y925), and total FAK. (E) Quantification of relative phosphorylation of Y397, Y861, and Y925 normalized for total FAK in each extract. (F) Similar quantification of Y407 and Y577. Error bars indicate the standard deviations ( n = 3). Symbols: ▪, pY397; ▾, pY407; ▪, pY577; ○, pY861; ▾, pY925.

    Techniques Used: Transformation Assay

    Adhesion-dependent control of cell growth rate in the absence of serum. Cells were plated on substrates for 48 h in serum-free medium. BrdU was added from 48 to 72 h, the cells were stained with anti-BrdU, and the proportion of BrdU positive nuclei was counted. In experiment 1, normal CEF were plated on polylysine (1-CEF PL) or fibronectin (1-CEF-Fn). In experiment 2, normal CEF were plated on normal (2-CEF-Fn) or cross-linked (2-CEF-X-Fn) fibronectin, and v-src-transformed CEF were plated on normal (2-src-Fn) or cross-linked (2-src-X-Fn) fibronectin. Error bars indicate the standard deviations ( n = 3).
    Figure Legend Snippet: Adhesion-dependent control of cell growth rate in the absence of serum. Cells were plated on substrates for 48 h in serum-free medium. BrdU was added from 48 to 72 h, the cells were stained with anti-BrdU, and the proportion of BrdU positive nuclei was counted. In experiment 1, normal CEF were plated on polylysine (1-CEF PL) or fibronectin (1-CEF-Fn). In experiment 2, normal CEF were plated on normal (2-CEF-Fn) or cross-linked (2-CEF-X-Fn) fibronectin, and v-src-transformed CEF were plated on normal (2-src-Fn) or cross-linked (2-src-X-Fn) fibronectin. Error bars indicate the standard deviations ( n = 3).

    Techniques Used: Staining, Transformation Assay

    v-src-transformed cells can assemble a fibronectin matrix. v-src-transformed CEF were incubated for 48 h on coated cover glasses. (A) Cross-linked fibronectin stained with anti-chicken fibronectin monoclonal antibody B3D6. (B) Same field as panel A but stained with ethidium homodimer. (C) Non-cross-linked fibronectin stained with B3D6. (D) Same field as panel C but stained with ethidium homodimer.
    Figure Legend Snippet: v-src-transformed cells can assemble a fibronectin matrix. v-src-transformed CEF were incubated for 48 h on coated cover glasses. (A) Cross-linked fibronectin stained with anti-chicken fibronectin monoclonal antibody B3D6. (B) Same field as panel A but stained with ethidium homodimer. (C) Non-cross-linked fibronectin stained with B3D6. (D) Same field as panel C but stained with ethidium homodimer.

    Techniques Used: Transformation Assay, Incubation, Staining

    Adhesion assays for normal and v-src-transformed CEF obtiained by using the spinning disk assay. (A) Mean adhesion strength for normal CEF and v-src-transformed CEF [CEF(SRA)] for adhesion to fibronectin (160 ng/cm 2 ) for 15 min in the absence or presence of CSAT antibody (∗;
    Figure Legend Snippet: Adhesion assays for normal and v-src-transformed CEF obtiained by using the spinning disk assay. (A) Mean adhesion strength for normal CEF and v-src-transformed CEF [CEF(SRA)] for adhesion to fibronectin (160 ng/cm 2 ) for 15 min in the absence or presence of CSAT antibody (∗;

    Techniques Used: Transformation Assay

    Chemical cross-linking of fibronectin-bound α5β1. Level of substrate-bound β1 and α5 integrins after 1 h of attachment of cells to fibronectin was detected by Western blot of β1 and α5 integrins. X-link, recovered integrin subunits after cleavage of the cross-linker; Supernatant, extract of non-cross-linked integrins; CEF, normal cells; SRA, v-src-transformed cells.
    Figure Legend Snippet: Chemical cross-linking of fibronectin-bound α5β1. Level of substrate-bound β1 and α5 integrins after 1 h of attachment of cells to fibronectin was detected by Western blot of β1 and α5 integrins. X-link, recovered integrin subunits after cleavage of the cross-linker; Supernatant, extract of non-cross-linked integrins; CEF, normal cells; SRA, v-src-transformed cells.

    Techniques Used: Western Blot, Transformation Assay

    Chemical cross-linking inhibits proteolytic removal of fibronectin. v-src-transformed CEF were plated on fibronectin-coated or chemically cross-linked fibronectin-coated cover glasses. (A) Cells plated for 48 h on cross-linked fibronectin and stained with HFN7.1 monoclonal antibody showing diffuse staining of the initial human fibronectin. (B) Same field as panel A but stained with ethidium homodimer. (C) Cells plated on normal fibronectin for 48 h and stained with HFN7.1 showing that most of the fibronectin has been removed by the cells. (D) Same field as panel C but stained with ethidium homodimer. (E and F) Quantification of residual fibronectin levels after 48 h by using HFN7.1 antibody (E) or rabbit anti-fibronectin (F). Fn, fibronectin only; X-Fn, cross-linked fibronectin only; Fn-C, fibronectin plus transformed cells; X-Fn-C, cross-linked fibronectin plus transformed cells . (G and H) Modified enzyme-linked immunosorbent assay showing the removal of normal (●) or cross-linked (○) fibronectin by transformed cells as a function of fibronectin density by using HFN7.1 antibody (G) and rabbit anti-fibronectin antibody (H). Error bars indicate the standard deviation ( n = 3).
    Figure Legend Snippet: Chemical cross-linking inhibits proteolytic removal of fibronectin. v-src-transformed CEF were plated on fibronectin-coated or chemically cross-linked fibronectin-coated cover glasses. (A) Cells plated for 48 h on cross-linked fibronectin and stained with HFN7.1 monoclonal antibody showing diffuse staining of the initial human fibronectin. (B) Same field as panel A but stained with ethidium homodimer. (C) Cells plated on normal fibronectin for 48 h and stained with HFN7.1 showing that most of the fibronectin has been removed by the cells. (D) Same field as panel C but stained with ethidium homodimer. (E and F) Quantification of residual fibronectin levels after 48 h by using HFN7.1 antibody (E) or rabbit anti-fibronectin (F). Fn, fibronectin only; X-Fn, cross-linked fibronectin only; Fn-C, fibronectin plus transformed cells; X-Fn-C, cross-linked fibronectin plus transformed cells . (G and H) Modified enzyme-linked immunosorbent assay showing the removal of normal (●) or cross-linked (○) fibronectin by transformed cells as a function of fibronectin density by using HFN7.1 antibody (G) and rabbit anti-fibronectin antibody (H). Error bars indicate the standard deviation ( n = 3).

    Techniques Used: Transformation Assay, Staining, Modification, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Mean fluorescence index (calculated as geometric mean integrin – geometric mean control/geometric mean control) as determined by flow cytometry for expression of β1 and β3 integrin after 48 h on normal fibronectin (black bars) or glutaraldehyde cross-linked fibronectin (white bars).
    Figure Legend Snippet: Mean fluorescence index (calculated as geometric mean integrin – geometric mean control/geometric mean control) as determined by flow cytometry for expression of β1 and β3 integrin after 48 h on normal fibronectin (black bars) or glutaraldehyde cross-linked fibronectin (white bars).

    Techniques Used: Fluorescence, Flow Cytometry, Cytometry, Expressing

    Effects of fibronectin cross-linking and hyaluronidase on the morphology of v-src transformed CEF. (A) Plated for 12 h in the absence of serum on cover glasses coated with fibronectin. (B) Plated for 12 h in the absence of serum on cover glasses coated with glutaraldehyde cross-linked fibronectin. (C) Plated for 48 h on fibronectin. (D) Plated for 48 h of glutaraldehyde cross-linked fibronectin. (E) Plated for 7 days on glutaraldehyde cross-linked fibronectin in the presence of hyaluronidase and phalloidin stained. (F) Corresponding phase micrograph (i.e., for panel E). (G) Plated for 7 days on fibronectin in the presence of hyaluronidase and phalloidin stained. (H) Corresponding phase micrograph (i.e., for panel G).
    Figure Legend Snippet: Effects of fibronectin cross-linking and hyaluronidase on the morphology of v-src transformed CEF. (A) Plated for 12 h in the absence of serum on cover glasses coated with fibronectin. (B) Plated for 12 h in the absence of serum on cover glasses coated with glutaraldehyde cross-linked fibronectin. (C) Plated for 48 h on fibronectin. (D) Plated for 48 h of glutaraldehyde cross-linked fibronectin. (E) Plated for 7 days on glutaraldehyde cross-linked fibronectin in the presence of hyaluronidase and phalloidin stained. (F) Corresponding phase micrograph (i.e., for panel E). (G) Plated for 7 days on fibronectin in the presence of hyaluronidase and phalloidin stained. (H) Corresponding phase micrograph (i.e., for panel G).

    Techniques Used: Transformation Assay, Staining

    2) Product Images from "hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2"

    Article Title: hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201301016

    hGAAP expression alters the number and size of focal adhesions. U2-OS cells transfected with siRNA (24 h) or plasmids encoding hGAAP were seeded onto fibronectin-coated slides. (A) Confocal images show cells transfected with vinculin-GFP (14 h), fixed, and stained with phalloidin–Alexa Fluor 568. (B) Summary results (means ± SEM from ≥25 cells for each condition) show numbers of focal adhesions per cell, determined by counting vinculin-GFP spots. (C) IRM images of U2-OS cells overexpressing hGAAP or hGAAP Ctmut, or transfected with the indicated siRNAs. Images are typical of three independent experiments. (D) Example of the image analysis used to determine focal adhesion number and size. Individual cells were imaged for IRM (a) and phalloidin staining (b). IRM images were then band pass filtered (c), and a threshold was imposed (d) to obtain the final images used to determine the number and size of the adhesions. (E and F) Summary results (means ± SEM from ≥20 cells for each condition) show numbers of adhesions per cell (E) and their areas (F). *, P
    Figure Legend Snippet: hGAAP expression alters the number and size of focal adhesions. U2-OS cells transfected with siRNA (24 h) or plasmids encoding hGAAP were seeded onto fibronectin-coated slides. (A) Confocal images show cells transfected with vinculin-GFP (14 h), fixed, and stained with phalloidin–Alexa Fluor 568. (B) Summary results (means ± SEM from ≥25 cells for each condition) show numbers of focal adhesions per cell, determined by counting vinculin-GFP spots. (C) IRM images of U2-OS cells overexpressing hGAAP or hGAAP Ctmut, or transfected with the indicated siRNAs. Images are typical of three independent experiments. (D) Example of the image analysis used to determine focal adhesion number and size. Individual cells were imaged for IRM (a) and phalloidin staining (b). IRM images were then band pass filtered (c), and a threshold was imposed (d) to obtain the final images used to determine the number and size of the adhesions. (E and F) Summary results (means ± SEM from ≥20 cells for each condition) show numbers of adhesions per cell (E) and their areas (F). *, P

    Techniques Used: Expressing, Transfection, Staining

    hGAAP stimulates calpain activity. (A) U2-OS cells were transfected with the CFP/YFP calpain FRET biosensor, plated on fibronectin, and imaged live over 20 min in both FRET and IRM channels. Typical examples show stills from videos. Right images show enlargements of boxed areas in left images. Bars, 5 µm. (B) Summary results from the images collected at t = 0 (means ± SEM, for > 10 cells) show the percentages of low-FRET pixels (ratio
    Figure Legend Snippet: hGAAP stimulates calpain activity. (A) U2-OS cells were transfected with the CFP/YFP calpain FRET biosensor, plated on fibronectin, and imaged live over 20 min in both FRET and IRM channels. Typical examples show stills from videos. Right images show enlargements of boxed areas in left images. Bars, 5 µm. (B) Summary results from the images collected at t = 0 (means ± SEM, for > 10 cells) show the percentages of low-FRET pixels (ratio

    Techniques Used: Activity Assay, Transfection

    hGAAP expression alters focal adhesion dynamics. (A and E) U2-OS cells overexpressing hGAAP (A) or transfected with siRNAs (E) were transfected with vinculin-GFP and seeded onto fibronectin-coated dishes. After 30 min, individual cells were imaged at 2-min intervals for 2 h. Representative frames are shown, with arrows highlighting a single typical adhesion for each cell line. Bars, 10 µm. (B–D and F–H) Summary results (means ± SEM, n = 6 cells, with 4–10 focal adhesions analyzed in each) show mean lifetimes of focal adhesions (B and F) and rate constants for their disassembly (C and G) and assembly (D and H). **, P
    Figure Legend Snippet: hGAAP expression alters focal adhesion dynamics. (A and E) U2-OS cells overexpressing hGAAP (A) or transfected with siRNAs (E) were transfected with vinculin-GFP and seeded onto fibronectin-coated dishes. After 30 min, individual cells were imaged at 2-min intervals for 2 h. Representative frames are shown, with arrows highlighting a single typical adhesion for each cell line. Bars, 10 µm. (B–D and F–H) Summary results (means ± SEM, n = 6 cells, with 4–10 focal adhesions analyzed in each) show mean lifetimes of focal adhesions (B and F) and rate constants for their disassembly (C and G) and assembly (D and H). **, P

    Techniques Used: Expressing, Transfection

    Overexpression of hGAAP increases the speed of cell migration. (A–F) U2-OS cells overexpressing hGAAP, hGAAP Ctmut, or control neo (A–C) or cells transfected with siRNA (D–F) were seeded at low density on fibronectin-coated dishes. Individual cells were imaged at 5-min intervals for 18 h. Tracks of individual cells ( n = 30) are shown in A and D. Migration rates (B and E) and persistence (where 1 represents a straight line of migration from start to finish; C and F) are shown as means ± SEM from 30 cells. **, P
    Figure Legend Snippet: Overexpression of hGAAP increases the speed of cell migration. (A–F) U2-OS cells overexpressing hGAAP, hGAAP Ctmut, or control neo (A–C) or cells transfected with siRNA (D–F) were seeded at low density on fibronectin-coated dishes. Individual cells were imaged at 5-min intervals for 18 h. Tracks of individual cells ( n = 30) are shown in A and D. Migration rates (B and E) and persistence (where 1 represents a straight line of migration from start to finish; C and F) are shown as means ± SEM from 30 cells. **, P

    Techniques Used: Over Expression, Migration, Transfection

    Inhibition of calpain reduces the effects of hGAAP on cell migration and spreading. (A and B) Cells treated with PD150606 (A) or ALLM (B) were seeded on fibronectin-coated slides and left to adhere and spread for different times. Cells were fixed, and cell size was measured for > 50 cells per condition. (C–F) Cells treated with PD150606 (C and D) or ALLM (E and F) were seeded at low density in fibronectin-coated dishes, and cells were imaged every 5 min for 18 h. Individual cell tracks are shown in C and E ( n = 25 cells), and cumulative migration speeds from multiple migration tracks are shown in D and F. (G–J) Cells expressing hGAAP, hGAAP Ctmut, or control neo were treated with calpain 2–specific siRNAs. (G) IB showing effectiveness of calpain 2 knockdown in cells expressing hGAAP, hGAAP Ctmut, or control neo, and treated with calpain 2–specific siRNAs. (H) Areas of cells (measured 60 h after calpain 2 (Capn2) siRNA transfection, > 50 cells per condition) seeded on fibronectin-coated slides. (I and J) Cells treated with calpain 2 siRNA were seeded at low density in fibronectin-coated dishes, and individual cells were imaged every 5 min for 18 h. Individual cell tracks are shown ( n = 25 cells; I), and cumulative migration speeds are shown (J). Data are representative of three experiments and are shown as mean ± SEM. *, P
    Figure Legend Snippet: Inhibition of calpain reduces the effects of hGAAP on cell migration and spreading. (A and B) Cells treated with PD150606 (A) or ALLM (B) were seeded on fibronectin-coated slides and left to adhere and spread for different times. Cells were fixed, and cell size was measured for > 50 cells per condition. (C–F) Cells treated with PD150606 (C and D) or ALLM (E and F) were seeded at low density in fibronectin-coated dishes, and cells were imaged every 5 min for 18 h. Individual cell tracks are shown in C and E ( n = 25 cells), and cumulative migration speeds from multiple migration tracks are shown in D and F. (G–J) Cells expressing hGAAP, hGAAP Ctmut, or control neo were treated with calpain 2–specific siRNAs. (G) IB showing effectiveness of calpain 2 knockdown in cells expressing hGAAP, hGAAP Ctmut, or control neo, and treated with calpain 2–specific siRNAs. (H) Areas of cells (measured 60 h after calpain 2 (Capn2) siRNA transfection, > 50 cells per condition) seeded on fibronectin-coated slides. (I and J) Cells treated with calpain 2 siRNA were seeded at low density in fibronectin-coated dishes, and individual cells were imaged every 5 min for 18 h. Individual cell tracks are shown ( n = 25 cells; I), and cumulative migration speeds are shown (J). Data are representative of three experiments and are shown as mean ± SEM. *, P

    Techniques Used: Inhibition, Migration, Expressing, Transfection

    3) Product Images from "Loss of Endogenous Bone Morphogenetic Protein-6 Aggravates Renal Fibrosis"

    Article Title: Loss of Endogenous Bone Morphogenetic Protein-6 Aggravates Renal Fibrosis

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2010.12.005

    BMP-6 deficiency is associated with increased numbers of circulating MFPCs, which do not, however, seem to incorporate into the kidney. Mononuclear cells were isolated from the spleen, and equal amounts were cultured on fibronectin-coated dishes to promote
    Figure Legend Snippet: BMP-6 deficiency is associated with increased numbers of circulating MFPCs, which do not, however, seem to incorporate into the kidney. Mononuclear cells were isolated from the spleen, and equal amounts were cultured on fibronectin-coated dishes to promote

    Techniques Used: Isolation, Cell Culture

    4) Product Images from "Mechanical phenotype of cancer cells: cell softening and loss of stiffness sensing"

    Article Title: Mechanical phenotype of cancer cells: cell softening and loss of stiffness sensing

    Journal: Oncotarget

    doi:

    The spatial distributions of cells cultured on stiffness gradient PA gels A representative spatial map of the distance from the center of the gradient PA gel versus ( A ) the elasticity (Pa) characterized by AFM, ( B ) the distribution of fibronectin of the gradient gel functionalized with (top and middle) or without (bottom) fibronectin (green) as demonstrated by the confocal Z-projection and Z-sectional images. ( C ) Mosaic crystal violet staining images of cells on gradient PA gels for three days. ( D ) The number of cells on each indicated area were counted and normalized to the total number of cells on the whole gradient PA gel. Scale bar = 1 mm.
    Figure Legend Snippet: The spatial distributions of cells cultured on stiffness gradient PA gels A representative spatial map of the distance from the center of the gradient PA gel versus ( A ) the elasticity (Pa) characterized by AFM, ( B ) the distribution of fibronectin of the gradient gel functionalized with (top and middle) or without (bottom) fibronectin (green) as demonstrated by the confocal Z-projection and Z-sectional images. ( C ) Mosaic crystal violet staining images of cells on gradient PA gels for three days. ( D ) The number of cells on each indicated area were counted and normalized to the total number of cells on the whole gradient PA gel. Scale bar = 1 mm.

    Techniques Used: Cell Culture, Staining

    Related Articles

    Incubation:

    Article Title: Nestin-Expressing Stem Cells Promote Nerve Growth in Long-Term 3-Dimensional Gelfoam(R)-Supported Histoculture
    Article Snippet: The isolated nerves and the spinal cord were washed in PBS three times before culture. .. Suspension culture of nestin-expressing cells of the sciatic nerve and sphere formation The excised sciatic nerve was cut into small pieces, which were incubated in DMEM-F12 (GIBCO/BRL), containing B-27 (GIBCO/BRL), N2 (GIBCO/BRL), 1% penicillin and streptomycin (GIBCO/BRL) and 20 ng/ml basic fibroblast growth factor (Millipore) at 37°C, 5% CO2 100% humidity in 24-well tissue-culture dishes (Sarstedt). ..

    Cell Culture:

    Article Title: Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor
    Article Snippet: MTT was purchased from Bio Basic (Markham Ontario, Canada). .. DMEM, Neurobasal medium, B27 cell culture supplement, and G418 were purchased from Life Technologies (Grand Island, NY, USA). ..

    other:

    Article Title: Strabismus regulates asymmetric cell divisions and cell fate determination in the mouse brain
    Article Snippet: Single cells were seeded at a density of 105 cells/cm2 in DME/F12 media containing N2 supplement , 10 ng/ml bFGF, 10 ng/ml EGF (Invitrogen), and 2% B27 supplement (Invitrogen).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher human fibronectin
    Effect of dextran on the culture, adhesion, and proliferation. 3 × 10 4 /cm 2 floating endothelial progenitor cells (EPCs) were cultured in medium with 5% dextran (A‐b and ‐e) and 10% dextran (A‐c and ‐f) or without dextran (A‐a and ‐d) on human <t>fibronectin‐coated</t> dishes. After 4 days (A‐a, ‐b, and ‐c) and 7 days (A‐d, ‐e, and ‐f) EPCs were observed by a phase contrast microscope (×10) (A). Dextran induced differentiation of circulating EPCs toward adhesive EPCs. Floating EPCs exposed to various densities of dextran for 24 h were cultured for 6 h and the adhesive cells were observed by a phase contrast microscope (×10) (B). EPCs exposed to dextran significantly increased adhesion. The number of adhesive cells per high‐power field (HPF) was counted (C). N = 3. Floating EPCs exposed to various density of dextran for 24 h were cultured for 24 h and the proliferation activity was measured (D). Dextran increased proliferation. N = 5. Data are means ± SD. ** P
    Human Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fibronectin/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human fibronectin - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    94
    Thermo Fisher fibronectin coated dishes
    hGAAP expression alters the number and size of focal adhesions. U2-OS cells transfected with siRNA (24 h) or plasmids encoding hGAAP were seeded onto <t>fibronectin-coated</t> slides. (A) Confocal images show cells transfected with vinculin-GFP (14 h), fixed, and stained with phalloidin–Alexa Fluor 568. (B) Summary results (means ± SEM from ≥25 cells for each condition) show numbers of focal adhesions per cell, determined by counting vinculin-GFP spots. (C) IRM images of U2-OS cells overexpressing hGAAP or hGAAP Ctmut, or transfected with the indicated siRNAs. Images are typical of three independent experiments. (D) Example of the image analysis used to determine focal adhesion number and size. Individual cells were imaged for IRM (a) and phalloidin staining (b). IRM images were then band pass filtered (c), and a threshold was imposed (d) to obtain the final images used to determine the number and size of the adhesions. (E and F) Summary results (means ± SEM from ≥20 cells for each condition) show numbers of adhesions per cell (E) and their areas (F). *, P
    Fibronectin Coated Dishes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin coated dishes/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fibronectin coated dishes - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    97
    Thermo Fisher fibronectin
    Phosphorylation of S106 is critical for Asef2-promoted cell migration. (A) HT1080 cells expressing GFP, GFP-Asef2, GFP-Asef2-S106A, or GFP-Asef2-S106D were plated on <t>fibronectin-coated</t> dishes and imaged using time-lapse microscopy. The migration of individual cells was tracked and analyzed. Wind-Rose plots depicting the migration tracks for individual cells are shown. (B) Migration speed was quantified for GFP-, GFP-Asef2-, GFP-Asef2-S106A-, and GFP-Asef2-S106D-expressing cells. Error bars represent s.e.m. for 66–159 cells from 4 to 9 independent experiments (*, p
    Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fibronectin - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    Image Search Results


    Effect of dextran on the culture, adhesion, and proliferation. 3 × 10 4 /cm 2 floating endothelial progenitor cells (EPCs) were cultured in medium with 5% dextran (A‐b and ‐e) and 10% dextran (A‐c and ‐f) or without dextran (A‐a and ‐d) on human fibronectin‐coated dishes. After 4 days (A‐a, ‐b, and ‐c) and 7 days (A‐d, ‐e, and ‐f) EPCs were observed by a phase contrast microscope (×10) (A). Dextran induced differentiation of circulating EPCs toward adhesive EPCs. Floating EPCs exposed to various densities of dextran for 24 h were cultured for 6 h and the adhesive cells were observed by a phase contrast microscope (×10) (B). EPCs exposed to dextran significantly increased adhesion. The number of adhesive cells per high‐power field (HPF) was counted (C). N = 3. Floating EPCs exposed to various density of dextran for 24 h were cultured for 24 h and the proliferation activity was measured (D). Dextran increased proliferation. N = 5. Data are means ± SD. ** P

    Journal: Physiological Reports

    Article Title: Dextran induces differentiation of circulating endothelial progenitor cells

    doi: 10.1002/phy2.261

    Figure Lengend Snippet: Effect of dextran on the culture, adhesion, and proliferation. 3 × 10 4 /cm 2 floating endothelial progenitor cells (EPCs) were cultured in medium with 5% dextran (A‐b and ‐e) and 10% dextran (A‐c and ‐f) or without dextran (A‐a and ‐d) on human fibronectin‐coated dishes. After 4 days (A‐a, ‐b, and ‐c) and 7 days (A‐d, ‐e, and ‐f) EPCs were observed by a phase contrast microscope (×10) (A). Dextran induced differentiation of circulating EPCs toward adhesive EPCs. Floating EPCs exposed to various densities of dextran for 24 h were cultured for 6 h and the adhesive cells were observed by a phase contrast microscope (×10) (B). EPCs exposed to dextran significantly increased adhesion. The number of adhesive cells per high‐power field (HPF) was counted (C). N = 3. Floating EPCs exposed to various density of dextran for 24 h were cultured for 24 h and the proliferation activity was measured (D). Dextran increased proliferation. N = 5. Data are means ± SD. ** P

    Article Snippet: 3 × 104 /cm2 ex vivo expanded EPCs were applied on culture dishes coated with 100 μg/mL solution of human fibronectin (GIBCO, Grand island, NY), and cultured in M199 medium (GIBCO) with 5% fetal bovine serum (FBS; JRH), and EGM2 (VEGF, fibroblast growth factor‐2, epidermal growth factor, insulin‐like growth factor‐1, and ascorbic acid; Clonetics) at 37°C under 5% CO2 atmosphere for 7 days.

    Techniques: Cell Culture, Microscopy, Activity Assay

    hGAAP expression alters the number and size of focal adhesions. U2-OS cells transfected with siRNA (24 h) or plasmids encoding hGAAP were seeded onto fibronectin-coated slides. (A) Confocal images show cells transfected with vinculin-GFP (14 h), fixed, and stained with phalloidin–Alexa Fluor 568. (B) Summary results (means ± SEM from ≥25 cells for each condition) show numbers of focal adhesions per cell, determined by counting vinculin-GFP spots. (C) IRM images of U2-OS cells overexpressing hGAAP or hGAAP Ctmut, or transfected with the indicated siRNAs. Images are typical of three independent experiments. (D) Example of the image analysis used to determine focal adhesion number and size. Individual cells were imaged for IRM (a) and phalloidin staining (b). IRM images were then band pass filtered (c), and a threshold was imposed (d) to obtain the final images used to determine the number and size of the adhesions. (E and F) Summary results (means ± SEM from ≥20 cells for each condition) show numbers of adhesions per cell (E) and their areas (F). *, P

    Journal: The Journal of Cell Biology

    Article Title: hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2

    doi: 10.1083/jcb.201301016

    Figure Lengend Snippet: hGAAP expression alters the number and size of focal adhesions. U2-OS cells transfected with siRNA (24 h) or plasmids encoding hGAAP were seeded onto fibronectin-coated slides. (A) Confocal images show cells transfected with vinculin-GFP (14 h), fixed, and stained with phalloidin–Alexa Fluor 568. (B) Summary results (means ± SEM from ≥25 cells for each condition) show numbers of focal adhesions per cell, determined by counting vinculin-GFP spots. (C) IRM images of U2-OS cells overexpressing hGAAP or hGAAP Ctmut, or transfected with the indicated siRNAs. Images are typical of three independent experiments. (D) Example of the image analysis used to determine focal adhesion number and size. Individual cells were imaged for IRM (a) and phalloidin staining (b). IRM images were then band pass filtered (c), and a threshold was imposed (d) to obtain the final images used to determine the number and size of the adhesions. (E and F) Summary results (means ± SEM from ≥20 cells for each condition) show numbers of adhesions per cell (E) and their areas (F). *, P

    Article Snippet: FACS analysis of cell surface integrins Cell surface expression of total and active β1 integrin in U2-OS cells seeded on 10 µg/ml fibronectin-coated dishes (Invitrogen) was determined by FACS (CyAn ADP multiple laser excitation; Dako).

    Techniques: Expressing, Transfection, Staining

    hGAAP stimulates calpain activity. (A) U2-OS cells were transfected with the CFP/YFP calpain FRET biosensor, plated on fibronectin, and imaged live over 20 min in both FRET and IRM channels. Typical examples show stills from videos. Right images show enlargements of boxed areas in left images. Bars, 5 µm. (B) Summary results from the images collected at t = 0 (means ± SEM, for > 10 cells) show the percentages of low-FRET pixels (ratio

    Journal: The Journal of Cell Biology

    Article Title: hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2

    doi: 10.1083/jcb.201301016

    Figure Lengend Snippet: hGAAP stimulates calpain activity. (A) U2-OS cells were transfected with the CFP/YFP calpain FRET biosensor, plated on fibronectin, and imaged live over 20 min in both FRET and IRM channels. Typical examples show stills from videos. Right images show enlargements of boxed areas in left images. Bars, 5 µm. (B) Summary results from the images collected at t = 0 (means ± SEM, for > 10 cells) show the percentages of low-FRET pixels (ratio

    Article Snippet: FACS analysis of cell surface integrins Cell surface expression of total and active β1 integrin in U2-OS cells seeded on 10 µg/ml fibronectin-coated dishes (Invitrogen) was determined by FACS (CyAn ADP multiple laser excitation; Dako).

    Techniques: Activity Assay, Transfection

    hGAAP expression alters focal adhesion dynamics. (A and E) U2-OS cells overexpressing hGAAP (A) or transfected with siRNAs (E) were transfected with vinculin-GFP and seeded onto fibronectin-coated dishes. After 30 min, individual cells were imaged at 2-min intervals for 2 h. Representative frames are shown, with arrows highlighting a single typical adhesion for each cell line. Bars, 10 µm. (B–D and F–H) Summary results (means ± SEM, n = 6 cells, with 4–10 focal adhesions analyzed in each) show mean lifetimes of focal adhesions (B and F) and rate constants for their disassembly (C and G) and assembly (D and H). **, P

    Journal: The Journal of Cell Biology

    Article Title: hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2

    doi: 10.1083/jcb.201301016

    Figure Lengend Snippet: hGAAP expression alters focal adhesion dynamics. (A and E) U2-OS cells overexpressing hGAAP (A) or transfected with siRNAs (E) were transfected with vinculin-GFP and seeded onto fibronectin-coated dishes. After 30 min, individual cells were imaged at 2-min intervals for 2 h. Representative frames are shown, with arrows highlighting a single typical adhesion for each cell line. Bars, 10 µm. (B–D and F–H) Summary results (means ± SEM, n = 6 cells, with 4–10 focal adhesions analyzed in each) show mean lifetimes of focal adhesions (B and F) and rate constants for their disassembly (C and G) and assembly (D and H). **, P

    Article Snippet: FACS analysis of cell surface integrins Cell surface expression of total and active β1 integrin in U2-OS cells seeded on 10 µg/ml fibronectin-coated dishes (Invitrogen) was determined by FACS (CyAn ADP multiple laser excitation; Dako).

    Techniques: Expressing, Transfection

    Overexpression of hGAAP increases the speed of cell migration. (A–F) U2-OS cells overexpressing hGAAP, hGAAP Ctmut, or control neo (A–C) or cells transfected with siRNA (D–F) were seeded at low density on fibronectin-coated dishes. Individual cells were imaged at 5-min intervals for 18 h. Tracks of individual cells ( n = 30) are shown in A and D. Migration rates (B and E) and persistence (where 1 represents a straight line of migration from start to finish; C and F) are shown as means ± SEM from 30 cells. **, P

    Journal: The Journal of Cell Biology

    Article Title: hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2

    doi: 10.1083/jcb.201301016

    Figure Lengend Snippet: Overexpression of hGAAP increases the speed of cell migration. (A–F) U2-OS cells overexpressing hGAAP, hGAAP Ctmut, or control neo (A–C) or cells transfected with siRNA (D–F) were seeded at low density on fibronectin-coated dishes. Individual cells were imaged at 5-min intervals for 18 h. Tracks of individual cells ( n = 30) are shown in A and D. Migration rates (B and E) and persistence (where 1 represents a straight line of migration from start to finish; C and F) are shown as means ± SEM from 30 cells. **, P

    Article Snippet: FACS analysis of cell surface integrins Cell surface expression of total and active β1 integrin in U2-OS cells seeded on 10 µg/ml fibronectin-coated dishes (Invitrogen) was determined by FACS (CyAn ADP multiple laser excitation; Dako).

    Techniques: Over Expression, Migration, Transfection

    Inhibition of calpain reduces the effects of hGAAP on cell migration and spreading. (A and B) Cells treated with PD150606 (A) or ALLM (B) were seeded on fibronectin-coated slides and left to adhere and spread for different times. Cells were fixed, and cell size was measured for > 50 cells per condition. (C–F) Cells treated with PD150606 (C and D) or ALLM (E and F) were seeded at low density in fibronectin-coated dishes, and cells were imaged every 5 min for 18 h. Individual cell tracks are shown in C and E ( n = 25 cells), and cumulative migration speeds from multiple migration tracks are shown in D and F. (G–J) Cells expressing hGAAP, hGAAP Ctmut, or control neo were treated with calpain 2–specific siRNAs. (G) IB showing effectiveness of calpain 2 knockdown in cells expressing hGAAP, hGAAP Ctmut, or control neo, and treated with calpain 2–specific siRNAs. (H) Areas of cells (measured 60 h after calpain 2 (Capn2) siRNA transfection, > 50 cells per condition) seeded on fibronectin-coated slides. (I and J) Cells treated with calpain 2 siRNA were seeded at low density in fibronectin-coated dishes, and individual cells were imaged every 5 min for 18 h. Individual cell tracks are shown ( n = 25 cells; I), and cumulative migration speeds are shown (J). Data are representative of three experiments and are shown as mean ± SEM. *, P

    Journal: The Journal of Cell Biology

    Article Title: hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2

    doi: 10.1083/jcb.201301016

    Figure Lengend Snippet: Inhibition of calpain reduces the effects of hGAAP on cell migration and spreading. (A and B) Cells treated with PD150606 (A) or ALLM (B) were seeded on fibronectin-coated slides and left to adhere and spread for different times. Cells were fixed, and cell size was measured for > 50 cells per condition. (C–F) Cells treated with PD150606 (C and D) or ALLM (E and F) were seeded at low density in fibronectin-coated dishes, and cells were imaged every 5 min for 18 h. Individual cell tracks are shown in C and E ( n = 25 cells), and cumulative migration speeds from multiple migration tracks are shown in D and F. (G–J) Cells expressing hGAAP, hGAAP Ctmut, or control neo were treated with calpain 2–specific siRNAs. (G) IB showing effectiveness of calpain 2 knockdown in cells expressing hGAAP, hGAAP Ctmut, or control neo, and treated with calpain 2–specific siRNAs. (H) Areas of cells (measured 60 h after calpain 2 (Capn2) siRNA transfection, > 50 cells per condition) seeded on fibronectin-coated slides. (I and J) Cells treated with calpain 2 siRNA were seeded at low density in fibronectin-coated dishes, and individual cells were imaged every 5 min for 18 h. Individual cell tracks are shown ( n = 25 cells; I), and cumulative migration speeds are shown (J). Data are representative of three experiments and are shown as mean ± SEM. *, P

    Article Snippet: FACS analysis of cell surface integrins Cell surface expression of total and active β1 integrin in U2-OS cells seeded on 10 µg/ml fibronectin-coated dishes (Invitrogen) was determined by FACS (CyAn ADP multiple laser excitation; Dako).

    Techniques: Inhibition, Migration, Expressing, Transfection

    Phosphorylation of S106 is critical for Asef2-promoted cell migration. (A) HT1080 cells expressing GFP, GFP-Asef2, GFP-Asef2-S106A, or GFP-Asef2-S106D were plated on fibronectin-coated dishes and imaged using time-lapse microscopy. The migration of individual cells was tracked and analyzed. Wind-Rose plots depicting the migration tracks for individual cells are shown. (B) Migration speed was quantified for GFP-, GFP-Asef2-, GFP-Asef2-S106A-, and GFP-Asef2-S106D-expressing cells. Error bars represent s.e.m. for 66–159 cells from 4 to 9 independent experiments (*, p

    Journal: Journal of Proteome Research

    Article Title: Phosphorylation of Serine 106 in Asef2 Regulates Cell Migration and Adhesion Turnover

    doi: 10.1021/pr5001384

    Figure Lengend Snippet: Phosphorylation of S106 is critical for Asef2-promoted cell migration. (A) HT1080 cells expressing GFP, GFP-Asef2, GFP-Asef2-S106A, or GFP-Asef2-S106D were plated on fibronectin-coated dishes and imaged using time-lapse microscopy. The migration of individual cells was tracked and analyzed. Wind-Rose plots depicting the migration tracks for individual cells are shown. (B) Migration speed was quantified for GFP-, GFP-Asef2-, GFP-Asef2-S106A-, and GFP-Asef2-S106D-expressing cells. Error bars represent s.e.m. for 66–159 cells from 4 to 9 independent experiments (*, p

    Article Snippet: Subsequently, the cells were plated on tissue culture dishes that were coated with 5 μg/mL fibronectin (diluted in Dulbecco’s Phosphate Buffered Saline (DPBS, Life Technologies, Grand Island, NY)) and allowed to adhere for 1 h at 37 °C.

    Techniques: Migration, Expressing, Time-lapse Microscopy