fibronectin coated dishes (Thermo Fisher)
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Name:
AarI AjiI Bpu10I ScaI PasI Buffer 10X
Description:
Thermo Scientific 10X Buffer AarI AjiI Bpu10I ScaI PasI is the optimal buffer recommended for use with AarI AjiI Bpu10I ScaI and PasI restriction enzymes and is premixed with BSA for enhanced stability To ensure consistent enzyme performance Thermo Scientific restriction enzyme buffers contain BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Multiple freeze thaw cycles of the buffers will not cause BSA precipitation Thermo Scientific restriction enzymes exhibit 100 of their certified activity in the recommended buffer
Catalog Number:
B27
Price:
None
Category:
Lab Reagents and Chemicals
Applications:
Cloning|Restriction Enzyme Cloning
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Structured Review
Thermo Fisher
fibronectin coated dishes
Thermo Scientific 10X Buffer AarI AjiI Bpu10I ScaI PasI is the optimal buffer recommended for use with AarI AjiI Bpu10I ScaI and PasI restriction enzymes and is premixed with BSA for enhanced stability To ensure consistent enzyme performance Thermo Scientific restriction enzyme buffers contain BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Multiple freeze thaw cycles of the buffers will not cause BSA precipitation Thermo Scientific restriction enzymes exhibit 100 of their certified activity in the recommended buffer
https://www.bioz.com/result/fibronectin coated dishes/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Thermo Scientific 10X Buffer AarI AjiI Bpu10I ScaI PasI is the optimal buffer recommended for use with AarI AjiI Bpu10I ScaI and PasI restriction enzymes and is premixed with BSA for enhanced stability To ensure consistent enzyme performance Thermo Scientific restriction enzyme buffers contain BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Multiple freeze thaw cycles of the buffers will not cause BSA precipitation Thermo Scientific restriction enzymes exhibit 100 of their certified activity in the recommended buffer
https://www.bioz.com/result/fibronectin coated dishes/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fibronectin coated dishes - by Bioz Stars,
2021-04
86/100 stars
Images
1) Product Images from "Transformation of Chicken Embryo Fibroblasts by v-src Uncouples ?1 Integrin-Mediated Outside-in but Not Inside-out Signaling"
Article Title: Transformation of Chicken Embryo Fibroblasts by v-src Uncouples ?1 Integrin-Mediated Outside-in but Not Inside-out Signaling
Journal: Molecular and Cellular Biology
doi: 10.1128/MCB.21.21.7295-7306.2001
Figure Legend Snippet: Specific induction of FAK phosphorylation by α5β1-mediated adhesion to fibronectin. Cells were serum starved overnight, trypsinized, neutralized with soybean trypsin inhibitor, and plated on fibronectin-coated plates at densities of 0, 50, 100, 150, and 350 ng/cm 2 for 60 min at room temperature (without phosphatase inhibitors). (A) Normal CEF extracts were analyzed by for phosphorylation of FAK(Y397), FAK(Y407), FAK(Y577), FAK(Y861), FAK(Y925), and total FAK protein. (B) Quantification of relative phosphorylation of Y397 in CEF; phosphorylated pY397/total FAK for each point. (C) Similar quantification of relative phosphorylation of Y861. (D) v-src-transformed CEF extracts were analyzed for specific phosphorylation of FAK(Y397), FAK(Y407), FAK(Y577), FAK(Y861), FAK(Y925), and total FAK. (E) Quantification of relative phosphorylation of Y397, Y861, and Y925 normalized for total FAK in each extract. (F) Similar quantification of Y407 and Y577. Error bars indicate the standard deviations ( n = 3). Symbols: ▪, pY397; ▾, pY407; ▪, pY577; ○, pY861; ▾, pY925.
Techniques Used: Transformation Assay
Figure Legend Snippet: Adhesion-dependent control of cell growth rate in the absence of serum. Cells were plated on substrates for 48 h in serum-free medium. BrdU was added from 48 to 72 h, the cells were stained with anti-BrdU, and the proportion of BrdU positive nuclei was counted. In experiment 1, normal CEF were plated on polylysine (1-CEF PL) or fibronectin (1-CEF-Fn). In experiment 2, normal CEF were plated on normal (2-CEF-Fn) or cross-linked (2-CEF-X-Fn) fibronectin, and v-src-transformed CEF were plated on normal (2-src-Fn) or cross-linked (2-src-X-Fn) fibronectin. Error bars indicate the standard deviations ( n = 3).
Techniques Used: Staining, Transformation Assay
Figure Legend Snippet: v-src-transformed cells can assemble a fibronectin matrix. v-src-transformed CEF were incubated for 48 h on coated cover glasses. (A) Cross-linked fibronectin stained with anti-chicken fibronectin monoclonal antibody B3D6. (B) Same field as panel A but stained with ethidium homodimer. (C) Non-cross-linked fibronectin stained with B3D6. (D) Same field as panel C but stained with ethidium homodimer.
Techniques Used: Transformation Assay, Incubation, Staining
![Adhesion assays for normal and v-src-transformed CEF obtiained by using the spinning disk assay. (A) Mean adhesion strength for normal CEF and v-src-transformed CEF [CEF(SRA)] for adhesion to fibronectin (160 ng/cm 2 ) for 15 min in the absence or presence of CSAT antibody (∗;](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC99904/bin/mb2110120002.jpg "... CEF and v-src-transformed CEF [CEF(SRA)] for adhesion to fibronectin (160 ng/cm 2 ) for 15 min in ...")
Figure Legend Snippet: Adhesion assays for normal and v-src-transformed CEF obtiained by using the spinning disk assay. (A) Mean adhesion strength for normal CEF and v-src-transformed CEF [CEF(SRA)] for adhesion to fibronectin (160 ng/cm 2 ) for 15 min in the absence or presence of CSAT antibody (∗;
Techniques Used: Transformation Assay
Figure Legend Snippet: Chemical cross-linking of fibronectin-bound α5β1. Level of substrate-bound β1 and α5 integrins after 1 h of attachment of cells to fibronectin was detected by Western blot of β1 and α5 integrins. X-link, recovered integrin subunits after cleavage of the cross-linker; Supernatant, extract of non-cross-linked integrins; CEF, normal cells; SRA, v-src-transformed cells.
Techniques Used: Western Blot, Transformation Assay
Figure Legend Snippet: Chemical cross-linking inhibits proteolytic removal of fibronectin. v-src-transformed CEF were plated on fibronectin-coated or chemically cross-linked fibronectin-coated cover glasses. (A) Cells plated for 48 h on cross-linked fibronectin and stained with HFN7.1 monoclonal antibody showing diffuse staining of the initial human fibronectin. (B) Same field as panel A but stained with ethidium homodimer. (C) Cells plated on normal fibronectin for 48 h and stained with HFN7.1 showing that most of the fibronectin has been removed by the cells. (D) Same field as panel C but stained with ethidium homodimer. (E and F) Quantification of residual fibronectin levels after 48 h by using HFN7.1 antibody (E) or rabbit anti-fibronectin (F). Fn, fibronectin only; X-Fn, cross-linked fibronectin only; Fn-C, fibronectin plus transformed cells; X-Fn-C, cross-linked fibronectin plus transformed cells . (G and H) Modified enzyme-linked immunosorbent assay showing the removal of normal (●) or cross-linked (○) fibronectin by transformed cells as a function of fibronectin density by using HFN7.1 antibody (G) and rabbit anti-fibronectin antibody (H). Error bars indicate the standard deviation ( n = 3).
Techniques Used: Transformation Assay, Staining, Modification, Enzyme-linked Immunosorbent Assay, Standard Deviation
Figure Legend Snippet: Mean fluorescence index (calculated as geometric mean integrin – geometric mean control/geometric mean control) as determined by flow cytometry for expression of β1 and β3 integrin after 48 h on normal fibronectin (black bars) or glutaraldehyde cross-linked fibronectin (white bars).
Techniques Used: Fluorescence, Flow Cytometry, Cytometry, Expressing
Figure Legend Snippet: Effects of fibronectin cross-linking and hyaluronidase on the morphology of v-src transformed CEF. (A) Plated for 12 h in the absence of serum on cover glasses coated with fibronectin. (B) Plated for 12 h in the absence of serum on cover glasses coated with glutaraldehyde cross-linked fibronectin. (C) Plated for 48 h on fibronectin. (D) Plated for 48 h of glutaraldehyde cross-linked fibronectin. (E) Plated for 7 days on glutaraldehyde cross-linked fibronectin in the presence of hyaluronidase and phalloidin stained. (F) Corresponding phase micrograph (i.e., for panel E). (G) Plated for 7 days on fibronectin in the presence of hyaluronidase and phalloidin stained. (H) Corresponding phase micrograph (i.e., for panel G).
Techniques Used: Transformation Assay, Staining
2) Product Images from "hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2"
Article Title: hGAAP promotes cell adhesion and migration via the stimulation of store-operated Ca2+ entry and calpain 2
Journal: The Journal of Cell Biology
doi: 10.1083/jcb.201301016
Figure Legend Snippet: hGAAP expression alters the number and size of focal adhesions. U2-OS cells transfected with siRNA (24 h) or plasmids encoding hGAAP were seeded onto fibronectin-coated slides. (A) Confocal images show cells transfected with vinculin-GFP (14 h), fixed, and stained with phalloidin–Alexa Fluor 568. (B) Summary results (means ± SEM from ≥25 cells for each condition) show numbers of focal adhesions per cell, determined by counting vinculin-GFP spots. (C) IRM images of U2-OS cells overexpressing hGAAP or hGAAP Ctmut, or transfected with the indicated siRNAs. Images are typical of three independent experiments. (D) Example of the image analysis used to determine focal adhesion number and size. Individual cells were imaged for IRM (a) and phalloidin staining (b). IRM images were then band pass filtered (c), and a threshold was imposed (d) to obtain the final images used to determine the number and size of the adhesions. (E and F) Summary results (means ± SEM from ≥20 cells for each condition) show numbers of adhesions per cell (E) and their areas (F). *, P
Techniques Used: Expressing, Transfection, Staining
Figure Legend Snippet: hGAAP stimulates calpain activity. (A) U2-OS cells were transfected with the CFP/YFP calpain FRET biosensor, plated on fibronectin, and imaged live over 20 min in both FRET and IRM channels. Typical examples show stills from videos. Right images show enlargements of boxed areas in left images. Bars, 5 µm. (B) Summary results from the images collected at t = 0 (means ± SEM, for > 10 cells) show the percentages of low-FRET pixels (ratio
Techniques Used: Activity Assay, Transfection
Figure Legend Snippet: hGAAP expression alters focal adhesion dynamics. (A and E) U2-OS cells overexpressing hGAAP (A) or transfected with siRNAs (E) were transfected with vinculin-GFP and seeded onto fibronectin-coated dishes. After 30 min, individual cells were imaged at 2-min intervals for 2 h. Representative frames are shown, with arrows highlighting a single typical adhesion for each cell line. Bars, 10 µm. (B–D and F–H) Summary results (means ± SEM, n = 6 cells, with 4–10 focal adhesions analyzed in each) show mean lifetimes of focal adhesions (B and F) and rate constants for their disassembly (C and G) and assembly (D and H). **, P
Techniques Used: Expressing, Transfection
Figure Legend Snippet: Overexpression of hGAAP increases the speed of cell migration. (A–F) U2-OS cells overexpressing hGAAP, hGAAP Ctmut, or control neo (A–C) or cells transfected with siRNA (D–F) were seeded at low density on fibronectin-coated dishes. Individual cells were imaged at 5-min intervals for 18 h. Tracks of individual cells ( n = 30) are shown in A and D. Migration rates (B and E) and persistence (where 1 represents a straight line of migration from start to finish; C and F) are shown as means ± SEM from 30 cells. **, P
Techniques Used: Over Expression, Migration, Transfection
Figure Legend Snippet: Inhibition of calpain reduces the effects of hGAAP on cell migration and spreading. (A and B) Cells treated with PD150606 (A) or ALLM (B) were seeded on fibronectin-coated slides and left to adhere and spread for different times. Cells were fixed, and cell size was measured for > 50 cells per condition. (C–F) Cells treated with PD150606 (C and D) or ALLM (E and F) were seeded at low density in fibronectin-coated dishes, and cells were imaged every 5 min for 18 h. Individual cell tracks are shown in C and E ( n = 25 cells), and cumulative migration speeds from multiple migration tracks are shown in D and F. (G–J) Cells expressing hGAAP, hGAAP Ctmut, or control neo were treated with calpain 2–specific siRNAs. (G) IB showing effectiveness of calpain 2 knockdown in cells expressing hGAAP, hGAAP Ctmut, or control neo, and treated with calpain 2–specific siRNAs. (H) Areas of cells (measured 60 h after calpain 2 (Capn2) siRNA transfection, > 50 cells per condition) seeded on fibronectin-coated slides. (I and J) Cells treated with calpain 2 siRNA were seeded at low density in fibronectin-coated dishes, and individual cells were imaged every 5 min for 18 h. Individual cell tracks are shown ( n = 25 cells; I), and cumulative migration speeds are shown (J). Data are representative of three experiments and are shown as mean ± SEM. *, P
Techniques Used: Inhibition, Migration, Expressing, Transfection
3) Product Images from "Loss of Endogenous Bone Morphogenetic Protein-6 Aggravates Renal Fibrosis"
Article Title: Loss of Endogenous Bone Morphogenetic Protein-6 Aggravates Renal Fibrosis
Journal: The American Journal of Pathology
doi: 10.1016/j.ajpath.2010.12.005
Figure Legend Snippet: BMP-6 deficiency is associated with increased numbers of circulating MFPCs, which do not, however, seem to incorporate into the kidney. Mononuclear cells were isolated from the spleen, and equal amounts were cultured on fibronectin-coated dishes to promote
Techniques Used: Isolation, Cell Culture
4) Product Images from "Mechanical phenotype of cancer cells: cell softening and loss of stiffness sensing"
Article Title: Mechanical phenotype of cancer cells: cell softening and loss of stiffness sensing
Journal: Oncotarget
doi:
Figure Legend Snippet: The spatial distributions of cells cultured on stiffness gradient PA gels A representative spatial map of the distance from the center of the gradient PA gel versus ( A ) the elasticity (Pa) characterized by AFM, ( B ) the distribution of fibronectin of the gradient gel functionalized with (top and middle) or without (bottom) fibronectin (green) as demonstrated by the confocal Z-projection and Z-sectional images. ( C ) Mosaic crystal violet staining images of cells on gradient PA gels for three days. ( D ) The number of cells on each indicated area were counted and normalized to the total number of cells on the whole gradient PA gel. Scale bar = 1 mm.
Techniques Used: Cell Culture, Staining
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