Journal: Bioactive Materials

Article Title: Slippery dopamine–fluoropolymer hybrid surface for improving biliary stent longevity

doi: 10.1016/j.bioactmat.2026.02.003

Figure Lengend Snippet: In vitro cell evaluations. (a, b) Fluorescence microscopic images of NIH 3T3 cells stained with a live/dead kit and corresponding quantitative analysis (n = 4) (scale bars, 100 μm). (c) Cytotoxicity analysis with NIT-3T3 cells using CCK-8 kit (n = 4). (d, e) Morphological analysis of NIH 3T3 cells stained for actin (red) and nucleus (blue), with fibroblast aspect ratio analysis (scale bars, 100 μm) (n = 4). (f) Schematic illustration demonstrating the selective application of ELFS coating to the target region. (g, h) Fluorescence images showing selective adhesion of NIH 3T3 and RAW 264.7 cells to ELFS-uncoated region (n = 4) (scale bars, 100 μm). (i, j) Optical images and quantification of adhered colony-forming units (CFUs) on non-coated and ELFS-coated plates after incubation in E. coli and S. aureus suspensions for 24 h (n = 4). (k) Sequential SEM images depicting biofilm formation on non-coated and ELFS- coated stent fragments (n = 3) (scale bars, 0.5 μm). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001). ns, not significant.

Article Snippet: The prepared stents were placed on the Transwell insert, and NIH-3T3 fibroblasts (ATCC CRL-1658; 0.5 × 10 5 cells mL −1 ) or human biliary epithelial SNU-1079 cells (Korean Cell Line Bank, KCLB No. 01079; 0.5 × 10 5 cells mL −1 ) were cultured in 2 mL of DMEM supplemented with 10% bovine calf serum and 1% penicillin–streptomycin.

Techniques: In Vitro, Fluorescence, Staining, CCK-8 Assay, Incubation