Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: Molecular medicine reports

Article Title: Downregulation of transgelin 2 promotes breast cancer metastasis by activating the reactive oxygen species/nuclear factor‑κB signaling pathway.

doi: 10.3892/mmr.2019.10643

Figure Lengend Snippet: Figure 3. Loss of TAGLN2 expression induces NF‑κB activation. (A) Luciferase activity of an NF‑κB‑luciferase reporter plasmid in TAGLN2‑overexpressing 293T cells was measured 48 h after stimulation with siRNA‑TAGLN2 or scramble siRNA; luciferase activity was normalized to that of Renilla. Data were representative of three independent experiments. (B) Immunoblotting of IκB, p50, p65/RelA and TAGLN2 expression in MDA‑MB‑231 and MCF‑7 cells transfected with pLKO.1‑shRNA‑TAGLN2 and pLKO.1‑shRNA‑scramble. The results of western blotting were representative of three independent experi- ments. (C) Cell migration was promoted in MDA‑MB‑231 cells transfected with pLKO.1‑shRNA‑TAGLN2 compared with cells treated with scramble shRNA. Treatment with 18 µM SN50 for 24 h reversed the effects of TAGLN2 downregulation on cell invasion. Data are representative of three independent experi- ments. *P<0.05 vs. scramble or as indicated. DMSO, dimethyl sulfoxide; NF‑κB, nuclear factor‑κB; shRNA, short hairpin RNA; siRNA, small interfering RNA; TAGLN2, transgelin 2.

Article Snippet: The membranes were probed with primary antibodies against PrdX1 (1:1,000; cat. no. ePr5433; epitomics, abcam, cambridge, uK), FlaG (1:500; cat. no. F7425; Sigma-aldrich, Merck KGaa), TaGln2 (1:1,000; cat. no. 60044-1-lg; ProteinTech Group, inc.), iκB (1:3,000; cat. no. 66418-1-lg; ProteinTech Group, inc.), p50 (1:200; cat. no. 15506-1-aP; ProteinTech Group, inc.), p65 (1:1,000; cat. no 10745-1-aP; ProteinTech Group, inc.), MMP1 (1:1,000; cat. no. 54376; cell Signaling Technology), MMP2 (1:1,000; cat. no. 4022; cell Signaling Technology), cXcr4 (1:1,000; cat. no. ab181020; abcam, cambridge, uK), lamin B1 (1:3,000; cat. no. 12987-1-aP; ProteinTech Group, inc.) and GaPdH (1:3,000; cat. no. 60004-1-lg; ProteinTech Group, Inc.) on the rocking table at 4 ̊C overnight. after washing, the membranes were incubated with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 60 min: Goat anti-rabbit igG (1:3,000; Sa00001-2; ProteinTech Group, inc.), Goat anti-Mouse igG (1:3,000; Sa00001-1; ProteinTech Group, inc.).

Techniques: Expressing, Activation Assay, Luciferase, Activity Assay, Plasmid Preparation, Western Blot, Transfection, Migration, shRNA, Small Interfering RNA

Journal: Polymers

Article Title: Effects of the Washing Time and Washing Solution on the Biocompatibility and Mechanical Properties of 3D Printed Dental Resin Materials

doi: 10.3390/polym13244410

Figure Lengend Snippet: Confocal laser scanning microscopy images of human gingival fibroblasts cultures (5 × 10 4 cells/mL) on 3D printed specimens washed with various solutions and for various times after 24 h of incubation.

Article Snippet: Primary human gingival fibroblasts (HGFs; PCS-201-018, ATCC, Manassas, VA, USA) were used with Dulbecco’s modified Eagle medium (WelGene, Daegu, Korea) containing 10% fetal bovine serum (Thermo Scientific, Waltham, MA, USA), 5% penicillin/streptomycin (100×, WelGene), and 5% MEM nonessential amino acid solution (100×, WelGene).

Techniques: Confocal Laser Scanning Microscopy, Incubation