Journal: eLife

Article Title: Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation

doi: 10.7554/eLife.52027

Figure Lengend Snippet:

Article Snippet: Antibody , anti-FGFR1 (monoclonal polyclonal) , Cell Signaling Technology D8E4 9740 , RRID: AB_11178519 , WB (1:1000).

Techniques: Transfection, Construct, Clone Assay, Plasmid Preparation, Electroporation, shRNA, Recombinant, Sequencing, Immunoprecipitation, Software

Journal: Oncotarget

Article Title: FGFR1/FOXM1 pathway: a key regulator of glioblastoma stem cells radioresistance and a prognosis biomarker

doi: 10.18632/oncotarget.25827

Figure Lengend Snippet: ( A ) Expression of FGFR1, FGFR2, FGFR3 and FGFR4 was analyzed by real time PCR in tumor cells derived from 2 human GBM biopsy specimens (GC1 and GC2) cultured as GSLC-enriched neurospheres. ( B ) Cells were transfected with 2 different FGFR1 siRNA (siFGFR1(6) or siFGFR1(11)) or 2 different shRNA targeting FGFR1 (shFGFR1(74) or shFGFR1(85)) or a scramble control (Ctl). FGFR1 mRNA expression was analyzed by real-time PCR. ( C ) Cells were transfected with siFGFR1(11) (FGFR1(-)) or scramble control (Ctl). 24 h post-transfection cells are irradiated (6 Gy). 48 h post-irradiation FGFR1 expression was analyzed by real-time PCR and western-blot. Image is representative of 3 independent experiments. Quantifications of 3 experiments are presented as means ± SD. *** p < 0.001; ** p < 0.01; * 0.01 < p < 0.05.

Article Snippet: Blots were probed with the following primary antibodies: anti-FGFR1 (D8E4) XP Rabbit (diluted 1:1000; #9740; Cell Signaling Technology), anti-FOXM1 (D12D5) XP Rabbit (diluted 1:1000; #5436; Cell Signaling Technology), anti-MELK (diluted 1:000; #2274, Cell Signaling Technology), anti-TCF8/ZEB1 (D80D3) Rabbit (diluted 1:000; #3396, Cell Signaling Technology), anti-GLI2 (C-10) (diluted 1:1000; #sc-271786, Santa Cruz biotechnology, Clinisciences), anti-TWIST1 (diluted at 2.5 μg/ml; LS-C30601, LSBio), anti-FOXN3/CHES1 (aa308-408) (diluted 1:1000; LS-C159675, LSBio), anti-Actin (1:20,000; Merk Millipore), anti-tubulin (diluted 1:000; #2146, Cell Signaling Technology).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Derivative Assay, Cell Culture, Transfection, shRNA, Irradiation, Western Blot

Journal: Oncotarget

Article Title: FGFR1/FOXM1 pathway: a key regulator of glioblastoma stem cells radioresistance and a prognosis biomarker

doi: 10.18632/oncotarget.25827

Figure Lengend Snippet: FGFR1 inhibition induces modification in FOX family gene expression

Article Snippet: Blots were probed with the following primary antibodies: anti-FGFR1 (D8E4) XP Rabbit (diluted 1:1000; #9740; Cell Signaling Technology), anti-FOXM1 (D12D5) XP Rabbit (diluted 1:1000; #5436; Cell Signaling Technology), anti-MELK (diluted 1:000; #2274, Cell Signaling Technology), anti-TCF8/ZEB1 (D80D3) Rabbit (diluted 1:000; #3396, Cell Signaling Technology), anti-GLI2 (C-10) (diluted 1:1000; #sc-271786, Santa Cruz biotechnology, Clinisciences), anti-TWIST1 (diluted at 2.5 μg/ml; LS-C30601, LSBio), anti-FOXN3/CHES1 (aa308-408) (diluted 1:1000; LS-C159675, LSBio), anti-Actin (1:20,000; Merk Millipore), anti-tubulin (diluted 1:000; #2146, Cell Signaling Technology).

Techniques: Inhibition, Modification

Journal: Oncotarget

Article Title: FGFR1/FOXM1 pathway: a key regulator of glioblastoma stem cells radioresistance and a prognosis biomarker

doi: 10.18632/oncotarget.25827

Figure Lengend Snippet: FGFR1/FOXM1/MELK/GLI2/ZEB1/TWIST1 set is a strong prognostic factor independently of clinical and biological parameters

Article Snippet: Blots were probed with the following primary antibodies: anti-FGFR1 (D8E4) XP Rabbit (diluted 1:1000; #9740; Cell Signaling Technology), anti-FOXM1 (D12D5) XP Rabbit (diluted 1:1000; #5436; Cell Signaling Technology), anti-MELK (diluted 1:000; #2274, Cell Signaling Technology), anti-TCF8/ZEB1 (D80D3) Rabbit (diluted 1:000; #3396, Cell Signaling Technology), anti-GLI2 (C-10) (diluted 1:1000; #sc-271786, Santa Cruz biotechnology, Clinisciences), anti-TWIST1 (diluted at 2.5 μg/ml; LS-C30601, LSBio), anti-FOXN3/CHES1 (aa308-408) (diluted 1:1000; LS-C159675, LSBio), anti-Actin (1:20,000; Merk Millipore), anti-tubulin (diluted 1:000; #2146, Cell Signaling Technology).

Techniques: Methylation