fetuin  (New England Biolabs)


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    New England Biolabs fetuin
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin - by Bioz Stars, 2023-03
    95/100 stars

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    fetuin  (New England Biolabs)


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    New England Biolabs fetuin
    Time courses for the treatment of glycoproteins (upper panel: <t>fetuin,</t> middle: alpha-1-acid glycoprotein, lower: haptoglobin) with sialidase (T = 0 min) and subsequently <t>with</t> <t>galactosidase</t> (T = 60 min). Error bars are standard deviations from triplicates.
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin - by Bioz Stars, 2023-03
    86/100 stars

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    1) Product Images from "Simplifying the detection and monitoring of protein glycosylation during in vitro glycoengineering"

    Article Title: Simplifying the detection and monitoring of protein glycosylation during in vitro glycoengineering

    Journal: Scientific Reports

    doi: 10.1038/s41598-023-27634-z

    Time courses for the treatment of glycoproteins (upper panel: fetuin, middle: alpha-1-acid glycoprotein, lower: haptoglobin) with sialidase (T = 0 min) and subsequently with galactosidase (T = 60 min). Error bars are standard deviations from triplicates.
    Figure Legend Snippet: Time courses for the treatment of glycoproteins (upper panel: fetuin, middle: alpha-1-acid glycoprotein, lower: haptoglobin) with sialidase (T = 0 min) and subsequently with galactosidase (T = 60 min). Error bars are standard deviations from triplicates.

    Techniques Used:

    fetuin mixture  (New England Biolabs)


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  • 86

    Structured Review

    New England Biolabs fetuin mixture
    Fetuin Mixture, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin mixture/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin mixture - by Bioz Stars, 2023-03
    86/100 stars

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    fetuin  (New England Biolabs)


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    New England Biolabs fetuin
    ( a ) HRP <t>(I),</t> <t>RNase</t> B (II) or <t>fetuin</t> (III) were incubated for various times with PNGase Rc or F at pH 3.5 or 7.5, respectively. Deglycosylation was performed at an E:S of 1:48 (M:M) and at 37 °C. Deglycosylation efficiencies were monitored by SDS-PAGE followed by coomassie staining at indicated time points. ( b ) m/z spectra of intact SIRPα after overnight incubation at 37 °C and an E:S of 1:45 with PNGase Rc (I) or PNGase F (II), at pH 2.5 or pH 7.4 respectively. The inlayer of the lower panel shows the charge-deconvoluted mass spectrum confirming the identity of SIRPα.
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin - by Bioz Stars, 2023-03
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    1) Product Images from "A novel PNGase Rc for improved protein N-deglycosylation in bioanalytics and HDX-MS epitope mapping under challenging conditions"

    Article Title: A novel PNGase Rc for improved protein N-deglycosylation in bioanalytics and HDX-MS epitope mapping under challenging conditions

    Journal: bioRxiv

    doi: 10.1101/2022.04.13.488165

    ( a ) HRP (I), RNase B (II) or fetuin (III) were incubated for various times with PNGase Rc or F at pH 3.5 or 7.5, respectively. Deglycosylation was performed at an E:S of 1:48 (M:M) and at 37 °C. Deglycosylation efficiencies were monitored by SDS-PAGE followed by coomassie staining at indicated time points. ( b ) m/z spectra of intact SIRPα after overnight incubation at 37 °C and an E:S of 1:45 with PNGase Rc (I) or PNGase F (II), at pH 2.5 or pH 7.4 respectively. The inlayer of the lower panel shows the charge-deconvoluted mass spectrum confirming the identity of SIRPα.
    Figure Legend Snippet: ( a ) HRP (I), RNase B (II) or fetuin (III) were incubated for various times with PNGase Rc or F at pH 3.5 or 7.5, respectively. Deglycosylation was performed at an E:S of 1:48 (M:M) and at 37 °C. Deglycosylation efficiencies were monitored by SDS-PAGE followed by coomassie staining at indicated time points. ( b ) m/z spectra of intact SIRPα after overnight incubation at 37 °C and an E:S of 1:45 with PNGase Rc (I) or PNGase F (II), at pH 2.5 or pH 7.4 respectively. The inlayer of the lower panel shows the charge-deconvoluted mass spectrum confirming the identity of SIRPα.

    Techniques Used: Incubation, SDS Page, Staining

    fetuin  (New England Biolabs)


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  • 95

    Structured Review

    New England Biolabs fetuin
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin - by Bioz Stars, 2023-03
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    fetuin  (New England Biolabs)


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    New England Biolabs fetuin
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin - by Bioz Stars, 2023-03
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    fetuin  (New England Biolabs)


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  • 86

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    New England Biolabs fetuin
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetuin - by Bioz Stars, 2023-03
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    fetuin  (New England Biolabs)


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    New England Biolabs fetuin
    Characterization of wild-type and D614G S glycoproteins in GALE/GALK2 293T cells. (A) The wild-type SARS-CoV-2 S glycoprotein (D614, with an aspartic acid residue at 614) and the D614G variant (G614, with a glycine residue at 614) were expressed in wild-type 293T cells (wt) or in GALE/GALK2 293T cells (ko) . Cell lysates were untreated (No Rx) or were treated with the <t>indicated</t> <t>glycosidase(s),</t> followed by Western blotting with a mouse antibody against S1 (upper) or a rabbit antibody against S2 (lower). The S glycoproteins, either untreated or treated with different glycosidases, are indicated by red arrows. (B) As a control, <t>fetuin,</t> which has N- and O-linked glycans, was treated with the indicated glycosidases. The SDS-polyacrylamide gel was stained with Coomassie blue. The different fetuin glycoforms are indicated by red arrows. The results shown are typical of those obtained in two independent experiments.
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Analysis of Glycosylation and Disulfide Bonding of Wild-Type SARS-CoV-2 Spike Glycoprotein"

    Article Title: Analysis of Glycosylation and Disulfide Bonding of Wild-Type SARS-CoV-2 Spike Glycoprotein

    Journal: Journal of Virology

    doi: 10.1128/jvi.01626-21

    Characterization of wild-type and D614G S glycoproteins in GALE/GALK2 293T cells. (A) The wild-type SARS-CoV-2 S glycoprotein (D614, with an aspartic acid residue at 614) and the D614G variant (G614, with a glycine residue at 614) were expressed in wild-type 293T cells (wt) or in GALE/GALK2 293T cells (ko) . Cell lysates were untreated (No Rx) or were treated with the indicated glycosidase(s), followed by Western blotting with a mouse antibody against S1 (upper) or a rabbit antibody against S2 (lower). The S glycoproteins, either untreated or treated with different glycosidases, are indicated by red arrows. (B) As a control, fetuin, which has N- and O-linked glycans, was treated with the indicated glycosidases. The SDS-polyacrylamide gel was stained with Coomassie blue. The different fetuin glycoforms are indicated by red arrows. The results shown are typical of those obtained in two independent experiments.
    Figure Legend Snippet: Characterization of wild-type and D614G S glycoproteins in GALE/GALK2 293T cells. (A) The wild-type SARS-CoV-2 S glycoprotein (D614, with an aspartic acid residue at 614) and the D614G variant (G614, with a glycine residue at 614) were expressed in wild-type 293T cells (wt) or in GALE/GALK2 293T cells (ko) . Cell lysates were untreated (No Rx) or were treated with the indicated glycosidase(s), followed by Western blotting with a mouse antibody against S1 (upper) or a rabbit antibody against S2 (lower). The S glycoproteins, either untreated or treated with different glycosidases, are indicated by red arrows. (B) As a control, fetuin, which has N- and O-linked glycans, was treated with the indicated glycosidases. The SDS-polyacrylamide gel was stained with Coomassie blue. The different fetuin glycoforms are indicated by red arrows. The results shown are typical of those obtained in two independent experiments.

    Techniques Used: Variant Assay, Western Blot, Staining

    fetuin protein  (New England Biolabs)


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    New England Biolabs fetuin protein
    Fetuin Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    fetuin  (New England Biolabs)


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    New England Biolabs fetuin
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
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    fetuin  (New England Biolabs)


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    New England Biolabs fetuin
    Fetuin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetuin/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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