fetuin after denaturation (New England Biolabs)
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Fetuin After Denaturation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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1) Product Images from "High-throughput analysis of N-glycans using AutoTip via glycoprotein immobilization"
Article Title: High-throughput analysis of N-glycans using AutoTip via glycoprotein immobilization
Journal: Scientific Reports
Figure Legend Snippet: Rate of glycoprotein immobilization and PNGase F release of N-glycans. ( a ) Fetuin was conjugated to Aminolink resin via reductive amination, whereas the unbound proteins in supernatant were measured by a BCA assay. Fetuin was quickly immobilized to the resin within 4 h; ( b ) N-glycans were released by PNGase F in 25 mM ammonium bicarbonate at 37 °C. The PNGase F digestion was complete within 2 h. Four measurement were made for each condition and error bar represented standard deviation.
Techniques Used: BIA-KA, Standard Deviation
Figure Legend Snippet: Modification of sialic acids using carbodiimide coupling on AutoTip. Fetuin protein was modified by p-Toluidine in the presence of EDC after immobilization on the beads (Four AutoTips). Sialylated N-glycan, S2H5N4, was used to monitor completion of p-Toluidine-EDC reaction. Without derivatization, MALDI-MS detected no sialic acid H5N4 ( a ) and two sialic acids S2H5N4 ( c ); For incomplete derivatization, MALDI-MS detected ( a ), ( c ), and p-Toluidine labeled two sialic acids S2H5N4 ( b ); When completely labeled, only ( b ) was detected by MALDI-MS.
Techniques Used: Modification, Labeling