fetal bovine serum  (Thermo Fisher)


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    Name:
    Fetal Bovine Serum dialyzed
    Description:
    Gibco fetal bovine sera offers excellent value for basic cell culture specialty research and specific assays earning the trust of researchers with consistent quality and award winning support that helps meet your research needs and budget requirementsSera Category SpecialtyOrigin United States Endotoxin level 50 EU ml Specification is checked and recorded levels routinely 10 EU ml Hemoglobin level 25 mg dl
    Catalog Number:
    26400044
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher fetal bovine serum
    Gibco fetal bovine sera offers excellent value for basic cell culture specialty research and specific assays earning the trust of researchers with consistent quality and award winning support that helps meet your research needs and budget requirementsSera Category SpecialtyOrigin United States Endotoxin level 50 EU ml Specification is checked and recorded levels routinely 10 EU ml Hemoglobin level 25 mg dl
    https://www.bioz.com/result/fetal bovine serum/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Monocyte chemoattractant protein 1 and fractalkine play opposite roles in angiogenesis via recruitment of different macrophage subtypes
    Article Snippet: PrimeScript RT Master Mix and DRR041A SYBR Premix Ex Taq (Perfect Real Time) were purchased from TAKARA bio (Dalian, China). .. RPMI-1640, DMEM cell culture medium, fetal bovine serum (FBS) and phosphate-buffered saline (PBS) were obtained from ThermoFisher Scientific (Pittsburgh, USA). .. Recombinant human granulocyte macrophage-colony stimulating factor (GM-CSF) protein was purchased from R & D Systems (Minneapolis, USA).

    Article Title: Nuclear receptor interaction protein, a coactivator of androgen receptors (AR), is regulated by AR and Sp1 to feed forward and activate its own gene expression through AR protein stability
    Article Snippet: Furthermore, we illustrate that NRIP enhances AR-induced NRIP and PSA gene expression in prostate cancer cells. .. Cell culture and drug treatments We maintained 293T cells in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM l -glutamine, 100 U/ml of streptomycin and 100 U/ml of penicillin (Invitrogen). .. LNCaP cells were grown in RPMI 1640 (Invitrogen) containing 10% FBS, l -glutamine and antibiotics.

    Article Title: Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility
    Article Snippet: .. Cell culture NR6WT cells expressing human EGF receptor (EGFR) were maintained in modified Eagle's medium-α containing (MEMα) 7.5% fetal bovine serum (FBS) and 1% of each of the following: penicillin/streptomycin, L-Glutamine, non-essential amino acids and sodium pyruvate (all from GIBCO). .. The medium contained 350 μg/ml of G418 as a selection agent for human EGFR.

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
    Article Snippet: .. Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C. .. 5’-nested PCR primers (PDHB -P1~P6, ) and common 3’ primer (PDHB -R, ) were used to amplify six serial deletion fragments of the 5’-flanking region.

    Modification:

    Article Title: Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions
    Article Snippet: Device Cell Culture Human epitheloid cervix carcinoma HeLa cells, human colorectal carcinoma HCT116 cells, and human choriocarcinoma BeWo cells were supplied by RIKEN BRC (Tsukuba, Japan). .. Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (HeLa and HCT116) or 15% (BeWo) fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and 1× antibiotic-antimycotic (Thermo Fisher Scientific). ..

    Article Title: Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility
    Article Snippet: .. Cell culture NR6WT cells expressing human EGF receptor (EGFR) were maintained in modified Eagle's medium-α containing (MEMα) 7.5% fetal bovine serum (FBS) and 1% of each of the following: penicillin/streptomycin, L-Glutamine, non-essential amino acids and sodium pyruvate (all from GIBCO). .. The medium contained 350 μg/ml of G418 as a selection agent for human EGFR.

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
    Article Snippet: .. Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C. .. 5’-nested PCR primers (PDHB -P1~P6, ) and common 3’ primer (PDHB -R, ) were used to amplify six serial deletion fragments of the 5’-flanking region.

    Expressing:

    Article Title: Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility
    Article Snippet: .. Cell culture NR6WT cells expressing human EGF receptor (EGFR) were maintained in modified Eagle's medium-α containing (MEMα) 7.5% fetal bovine serum (FBS) and 1% of each of the following: penicillin/streptomycin, L-Glutamine, non-essential amino acids and sodium pyruvate (all from GIBCO). .. The medium contained 350 μg/ml of G418 as a selection agent for human EGFR.

    Transfection:

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
    Article Snippet: .. Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C. .. 5’-nested PCR primers (PDHB -P1~P6, ) and common 3’ primer (PDHB -R, ) were used to amplify six serial deletion fragments of the 5’-flanking region.

    Reporter Assay:

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
    Article Snippet: .. Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C. .. 5’-nested PCR primers (PDHB -P1~P6, ) and common 3’ primer (PDHB -R, ) were used to amplify six serial deletion fragments of the 5’-flanking region.

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  • 97
    Thermo Fisher g418
    Genetic analysis of mutant subgenomic replicons targeting the 5BSL3.2 structure. The silent mutations introduced into each construct are shown. Mutant 5BSL3.2 mutA contains mutations throughout the structure. 5BSL3.2 mutB contains the same changes as mutA except for the mutations in the terminal loop. Mutant 5BSL3.2 C has changes in both the terminal and internal loops, which are separated in mutD and mutE, respectively. The effects of these changes were examined by <t>G418</t> drug selection. Plates with 10 6 transfected cells are shown.
    G418, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g418/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g418 - by Bioz Stars, 2021-04
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    97
    Thermo Fisher heat inactivated fetal bovine serum fbs
    Shockwave treatment activates focal adhesion kinase (FAK) in Jurkat T-cells. After shockwave treatment at 0.18 mJ/mm 2 with 0 (control) or the indicated numbers of impulses, Jurkat T-cells (10 6 /ml) were cultured in 1 ml <t>RPMI-1640</t> medium containing 10% <t>FBS</t> in a 12-well plate for 3 min, and FAK activation was determined by immunoblotting with antibodies recognizing FAK phosphorylated on residues Tyr397 or Tyr576/577 or antibodies recognizing the activated and inactive forms of FAK. Band intensities were analyzed and ratios between activated and total FAK were used to calculate FAK Tyr397 or 576/577 activation. Representative Western blots of 6 different experiments are shown and data were averaged in the bar graph ( n = 6, means ± SD).
    Heat Inactivated Fetal Bovine Serum Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat inactivated fetal bovine serum fbs/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heat inactivated fetal bovine serum fbs - by Bioz Stars, 2021-04
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    99
    Thermo Fisher fetal bovine serum fbs
    Effects of 3-DSC on reporter gene assay. WNT reporter NIH3T3 cells permanently transfected with TCF/LEF-luciferase construct or HEK293 cells stably transfected with STAT3-luciferase constructs were seeded at 2×10 4 cells in 96-well plate and HEK293 cells permanently transfected with IL-4R site-TKluc/STAT6 contained IL-4 receptor site were seeded at 1×10 4 cells in each well of a 96-well plate in <t>DMEM</t> containing 5% <t>FBS</t> for 24 h. (A) TCF/LEF reporter gene assay: cells were treated with 3-DSC (0.01–10 μM) for 24 h. (B) IL-6 induced STAT3. (C) IL-4 induced STAT6 reporter gene assay as determined by luciferase activity. Each assay is representative for 3 experiments. The asterisk indicates a significant statistical significance ( * p
    Fetal Bovine Serum Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal bovine serum fbs/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum fbs - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Genetic analysis of mutant subgenomic replicons targeting the 5BSL3.2 structure. The silent mutations introduced into each construct are shown. Mutant 5BSL3.2 mutA contains mutations throughout the structure. 5BSL3.2 mutB contains the same changes as mutA except for the mutations in the terminal loop. Mutant 5BSL3.2 C has changes in both the terminal and internal loops, which are separated in mutD and mutE, respectively. The effects of these changes were examined by G418 drug selection. Plates with 10 6 transfected cells are shown.

    Journal: Journal of Virology

    Article Title: A cis-Acting Replication Element in the Sequence Encoding the NS5B RNA-Dependent RNA Polymerase Is Required for Hepatitis C Virus RNA Replication

    doi: 10.1128/JVI.78.3.1352-1366.2004

    Figure Lengend Snippet: Genetic analysis of mutant subgenomic replicons targeting the 5BSL3.2 structure. The silent mutations introduced into each construct are shown. Mutant 5BSL3.2 mutA contains mutations throughout the structure. 5BSL3.2 mutB contains the same changes as mutA except for the mutations in the terminal loop. Mutant 5BSL3.2 C has changes in both the terminal and internal loops, which are separated in mutD and mutE, respectively. The effects of these changes were examined by G418 drug selection. Plates with 10 6 transfected cells are shown.

    Article Snippet: After 48 h, the medium was changed to DMEM-10% FBS supplemented with G418 (Geneticin; Gibco Life Technologies) at 1 mg/ml.

    Techniques: Mutagenesis, Construct, Selection, Transfection

    Attempts to rescue the 5BSL3.2 mutant subgenomic replicon by adding a sequence harboring the cruciform structure. (A) Effects of an additional copy of the cruciform between the Neo r gene and the EMCV IRES. Schematic diagrams of the constructs tested are shown. The phenotype of these mutant constructs was compared based on G418 transduction efficiency. (a) Parental subgenomic replicon, Con1/SG-Neo (I); (b) Con1/SG-Neo (I) with a Bsi WI restriction site inserted after the Neo stop codon; (c) wild-type copy of the cruciform inserted after the Neo r gene; (d) mutant (mutA) copy of the cruciform on the parental background; (e) mutant (mutA) copy of the cruciform inserted in the context of 5BSL3.2 mutA; (f) wild-type copy of the cruciform inserted in the context of 5BSL3.2 mutA. (B) Effects of inserting an additional copy of the cruciform after the polyprotein stop codon. A schematic diagram and a crystal violet-stained plate indicating G418 transduction efficiency are shown for each construct. (a) Parental replicon; (b) 5BSL3.2 mutB; (c) parental replicon with an additional wild-type cruciform in the 3′ NTR; (d) 5BSL3.2 mutB with an additional mutB cruciform in the 3′ NTR; (e) 5BSL3.2 mutB with an additional wild-type cruciform in the 3′ NTR.

    Journal: Journal of Virology

    Article Title: A cis-Acting Replication Element in the Sequence Encoding the NS5B RNA-Dependent RNA Polymerase Is Required for Hepatitis C Virus RNA Replication

    doi: 10.1128/JVI.78.3.1352-1366.2004

    Figure Lengend Snippet: Attempts to rescue the 5BSL3.2 mutant subgenomic replicon by adding a sequence harboring the cruciform structure. (A) Effects of an additional copy of the cruciform between the Neo r gene and the EMCV IRES. Schematic diagrams of the constructs tested are shown. The phenotype of these mutant constructs was compared based on G418 transduction efficiency. (a) Parental subgenomic replicon, Con1/SG-Neo (I); (b) Con1/SG-Neo (I) with a Bsi WI restriction site inserted after the Neo stop codon; (c) wild-type copy of the cruciform inserted after the Neo r gene; (d) mutant (mutA) copy of the cruciform on the parental background; (e) mutant (mutA) copy of the cruciform inserted in the context of 5BSL3.2 mutA; (f) wild-type copy of the cruciform inserted in the context of 5BSL3.2 mutA. (B) Effects of inserting an additional copy of the cruciform after the polyprotein stop codon. A schematic diagram and a crystal violet-stained plate indicating G418 transduction efficiency are shown for each construct. (a) Parental replicon; (b) 5BSL3.2 mutB; (c) parental replicon with an additional wild-type cruciform in the 3′ NTR; (d) 5BSL3.2 mutB with an additional mutB cruciform in the 3′ NTR; (e) 5BSL3.2 mutB with an additional wild-type cruciform in the 3′ NTR.

    Article Snippet: After 48 h, the medium was changed to DMEM-10% FBS supplemented with G418 (Geneticin; Gibco Life Technologies) at 1 mg/ml.

    Techniques: Mutagenesis, Sequencing, Construct, Transduction, Staining

    Effects of silent mutations in stem-loop structures on replication of the subgenomic replicons. (A) Transduction efficiencies of the mutant constructs in a G418 drug selection assay. After electroporation, 10 6 , 10 5 , or 10 4 transfected cells were plated with feeders as described in Materials and Methods. After selection for 3 weeks, G418-resistant colonies were stained with crystal violet. Approximate transduction efficiencies (in CFU per microgram of RNA) are indicated. (B) Effects of mutations on HCV RNA levels at various times after transfection. Cells were harvested on day 1, 2, 3, 4, and 5 postelectroporation, and total RNA was harvested. One hundred nanograms of total RNA was analyzed by real-time RT-PCR (see Materials and Methods). HCV RNA was normalized to glyceraldehyde-3-phosphate dehydrogenase RNA. Similar results were obtained in two independent repetitions of the experiment.

    Journal: Journal of Virology

    Article Title: A cis-Acting Replication Element in the Sequence Encoding the NS5B RNA-Dependent RNA Polymerase Is Required for Hepatitis C Virus RNA Replication

    doi: 10.1128/JVI.78.3.1352-1366.2004

    Figure Lengend Snippet: Effects of silent mutations in stem-loop structures on replication of the subgenomic replicons. (A) Transduction efficiencies of the mutant constructs in a G418 drug selection assay. After electroporation, 10 6 , 10 5 , or 10 4 transfected cells were plated with feeders as described in Materials and Methods. After selection for 3 weeks, G418-resistant colonies were stained with crystal violet. Approximate transduction efficiencies (in CFU per microgram of RNA) are indicated. (B) Effects of mutations on HCV RNA levels at various times after transfection. Cells were harvested on day 1, 2, 3, 4, and 5 postelectroporation, and total RNA was harvested. One hundred nanograms of total RNA was analyzed by real-time RT-PCR (see Materials and Methods). HCV RNA was normalized to glyceraldehyde-3-phosphate dehydrogenase RNA. Similar results were obtained in two independent repetitions of the experiment.

    Article Snippet: After 48 h, the medium was changed to DMEM-10% FBS supplemented with G418 (Geneticin; Gibco Life Technologies) at 1 mg/ml.

    Techniques: Transduction, Mutagenesis, Construct, Selection, Electroporation, Transfection, Staining, Quantitative RT-PCR

    Mutational analysis of the terminal and internal loops of 5BSL3.2. (A) One-, two-, or three-nucleotide substitutions were introduced at specific positions in the 5BSL3.2 structure, as indicated. (B) Effects of these mutations on G418 transduction efficiency. Plates with 10 6 transfected cells are shown.

    Journal: Journal of Virology

    Article Title: A cis-Acting Replication Element in the Sequence Encoding the NS5B RNA-Dependent RNA Polymerase Is Required for Hepatitis C Virus RNA Replication

    doi: 10.1128/JVI.78.3.1352-1366.2004

    Figure Lengend Snippet: Mutational analysis of the terminal and internal loops of 5BSL3.2. (A) One-, two-, or three-nucleotide substitutions were introduced at specific positions in the 5BSL3.2 structure, as indicated. (B) Effects of these mutations on G418 transduction efficiency. Plates with 10 6 transfected cells are shown.

    Article Snippet: After 48 h, the medium was changed to DMEM-10% FBS supplemented with G418 (Geneticin; Gibco Life Technologies) at 1 mg/ml.

    Techniques: Transduction, Transfection

    Shockwave treatment activates focal adhesion kinase (FAK) in Jurkat T-cells. After shockwave treatment at 0.18 mJ/mm 2 with 0 (control) or the indicated numbers of impulses, Jurkat T-cells (10 6 /ml) were cultured in 1 ml RPMI-1640 medium containing 10% FBS in a 12-well plate for 3 min, and FAK activation was determined by immunoblotting with antibodies recognizing FAK phosphorylated on residues Tyr397 or Tyr576/577 or antibodies recognizing the activated and inactive forms of FAK. Band intensities were analyzed and ratios between activated and total FAK were used to calculate FAK Tyr397 or 576/577 activation. Representative Western blots of 6 different experiments are shown and data were averaged in the bar graph ( n = 6, means ± SD).

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Shockwaves increase T-cell proliferation and IL-2 expression through ATP release, P2X7 receptors, and FAK activation

    doi: 10.1152/ajpcell.00342.2009

    Figure Lengend Snippet: Shockwave treatment activates focal adhesion kinase (FAK) in Jurkat T-cells. After shockwave treatment at 0.18 mJ/mm 2 with 0 (control) or the indicated numbers of impulses, Jurkat T-cells (10 6 /ml) were cultured in 1 ml RPMI-1640 medium containing 10% FBS in a 12-well plate for 3 min, and FAK activation was determined by immunoblotting with antibodies recognizing FAK phosphorylated on residues Tyr397 or Tyr576/577 or antibodies recognizing the activated and inactive forms of FAK. Band intensities were analyzed and ratios between activated and total FAK were used to calculate FAK Tyr397 or 576/577 activation. Representative Western blots of 6 different experiments are shown and data were averaged in the bar graph ( n = 6, means ± SD).

    Article Snippet: DMEM, heat-inactivated fetal bovine serum (FBS), and RPMI-1640 were from GIBCO (Invitrogen, Tulsa, OK).

    Techniques: Cell Culture, Activation Assay, Western Blot

    Internalization of immunoRNases into tumor cells expressing different levels of ErbB2. Analysis by western blotting of lysates from SKBR3 cells (high ErbB2 expression) or JIMT-1 cells (low ErbB2 expression) untreated (control) or treated with Erb–HP-RNase

    Journal: Protein Engineering, Design and Selection

    Article Title: Effects of a second-generation human anti-ErbB2 ImmunoRNase on trastuzumab-resistant tumors and cardiac cells

    doi: 10.1093/protein/gzt065

    Figure Lengend Snippet: Internalization of immunoRNases into tumor cells expressing different levels of ErbB2. Analysis by western blotting of lysates from SKBR3 cells (high ErbB2 expression) or JIMT-1 cells (low ErbB2 expression) untreated (control) or treated with Erb–HP-RNase

    Article Snippet: Media were supplemented with 10% (7.5% for JIMT-1) heat-inactivated fetal bovine serum, penicillin (100 UI ml–1 ), streptomycin (100 μg ml–1 ) and 2 mM glutamine (all from Gibco, BRL) in a humidified atmosphere of 95% air and 5% CO2 at 37°C.

    Techniques: Expressing, Western Blot

    In vivo effects of Erb–HP-DDADD-RNase or Erb–HP-RNase on trastuzumab-resistant JIMT-1 tumors induced in mice. Treated mice ( n = 5) were injected with Erb–HP-RNase (empty circles) or Erb–HP-DDADD-RNase (black circles) at

    Journal: Protein Engineering, Design and Selection

    Article Title: Effects of a second-generation human anti-ErbB2 ImmunoRNase on trastuzumab-resistant tumors and cardiac cells

    doi: 10.1093/protein/gzt065

    Figure Lengend Snippet: In vivo effects of Erb–HP-DDADD-RNase or Erb–HP-RNase on trastuzumab-resistant JIMT-1 tumors induced in mice. Treated mice ( n = 5) were injected with Erb–HP-RNase (empty circles) or Erb–HP-DDADD-RNase (black circles) at

    Article Snippet: Media were supplemented with 10% (7.5% for JIMT-1) heat-inactivated fetal bovine serum, penicillin (100 UI ml–1 ), streptomycin (100 μg ml–1 ) and 2 mM glutamine (all from Gibco, BRL) in a humidified atmosphere of 95% air and 5% CO2 at 37°C.

    Techniques: In Vivo, Mouse Assay, Injection

    Toxicity of immunoRNases for tumor cells in vitro . Dose–response curves for gastric NCI-N87 (triangles), MKN-7 (circles) and AGS (squares) cell lines, as well as to breast SKBR3 (rhomboids) and trastuzumab-resistant JIMT-1 (crosses) cell lines,

    Journal: Protein Engineering, Design and Selection

    Article Title: Effects of a second-generation human anti-ErbB2 ImmunoRNase on trastuzumab-resistant tumors and cardiac cells

    doi: 10.1093/protein/gzt065

    Figure Lengend Snippet: Toxicity of immunoRNases for tumor cells in vitro . Dose–response curves for gastric NCI-N87 (triangles), MKN-7 (circles) and AGS (squares) cell lines, as well as to breast SKBR3 (rhomboids) and trastuzumab-resistant JIMT-1 (crosses) cell lines,

    Article Snippet: Media were supplemented with 10% (7.5% for JIMT-1) heat-inactivated fetal bovine serum, penicillin (100 UI ml–1 ), streptomycin (100 μg ml–1 ) and 2 mM glutamine (all from Gibco, BRL) in a humidified atmosphere of 95% air and 5% CO2 at 37°C.

    Techniques: In Vitro

    Effects of 3-DSC on reporter gene assay. WNT reporter NIH3T3 cells permanently transfected with TCF/LEF-luciferase construct or HEK293 cells stably transfected with STAT3-luciferase constructs were seeded at 2×10 4 cells in 96-well plate and HEK293 cells permanently transfected with IL-4R site-TKluc/STAT6 contained IL-4 receptor site were seeded at 1×10 4 cells in each well of a 96-well plate in DMEM containing 5% FBS for 24 h. (A) TCF/LEF reporter gene assay: cells were treated with 3-DSC (0.01–10 μM) for 24 h. (B) IL-6 induced STAT3. (C) IL-4 induced STAT6 reporter gene assay as determined by luciferase activity. Each assay is representative for 3 experiments. The asterisk indicates a significant statistical significance ( * p

    Journal: Biomolecules & Therapeutics

    Article Title: 3-Deoxysappanchalcone Promotes Proliferation of Human Hair Follicle Dermal Papilla Cells and Hair Growth in C57BL/6 Mice by Modulating WNT/β-Catenin and STAT Signaling

    doi: 10.4062/biomolther.2016.183

    Figure Lengend Snippet: Effects of 3-DSC on reporter gene assay. WNT reporter NIH3T3 cells permanently transfected with TCF/LEF-luciferase construct or HEK293 cells stably transfected with STAT3-luciferase constructs were seeded at 2×10 4 cells in 96-well plate and HEK293 cells permanently transfected with IL-4R site-TKluc/STAT6 contained IL-4 receptor site were seeded at 1×10 4 cells in each well of a 96-well plate in DMEM containing 5% FBS for 24 h. (A) TCF/LEF reporter gene assay: cells were treated with 3-DSC (0.01–10 μM) for 24 h. (B) IL-6 induced STAT3. (C) IL-4 induced STAT6 reporter gene assay as determined by luciferase activity. Each assay is representative for 3 experiments. The asterisk indicates a significant statistical significance ( * p

    Article Snippet: DMEM and fetal bovine serum (FBS) were procured from Invitrogen (Carlsbad, CA, USA).

    Techniques: Reporter Gene Assay, Transfection, Luciferase, Construct, Stable Transfection, Activity Assay