fetal bovine serum  (thermo fisher)


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    Name:
    Fetal Bovine Serum dialyzed
    Description:
    Gibco fetal bovine sera offers excellent value for basic cell culture specialty research and specific assays earning the trust of researchers with consistent quality and award winning support that helps meet your research needs and budget requirementsSera Category SpecialtyOrigin United States Endotoxin level 50 EU ml Specification is checked and recorded levels routinely 10 EU ml Hemoglobin level 25 mg dl
    Catalog Number:
    26400044
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    thermo fisher fetal bovine serum
    Gibco fetal bovine sera offers excellent value for basic cell culture specialty research and specific assays earning the trust of researchers with consistent quality and award winning support that helps meet your research needs and budget requirementsSera Category SpecialtyOrigin United States Endotoxin level 50 EU ml Specification is checked and recorded levels routinely 10 EU ml Hemoglobin level 25 mg dl
    https://www.bioz.com/result/fetal bovine serum/product/thermo fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
    Article Snippet: .. Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C. .. 5’-nested PCR primers (PDHB -P1~P6, ) and common 3’ primer (PDHB -R, ) were used to amplify six serial deletion fragments of the 5’-flanking region.

    Article Title: STAT3 Undergoes Acetylation-dependent Mitochondrial Translocation to Regulate Pyruvate Metabolism
    Article Snippet: .. Fu), were cultured in 90% DMEM, 10% FBS (Gibco, #10099-141), 100 μg/ ml penicillin (Life Technologies, #15140-155) and 100 μg/ ml streptomycin (Life Technologies, #15140–122) at 37 °C and 5% CO2 . .. Chemicals, including NAM (A2984), TSA (T1952), DAPI (5D8417), Oil red O (O0625), insulin (I3536), a glucose assay kit (GAGO20), an α-ketoglutaric acid assay kit (MAK054), acetyl-CoA assay kit (MAK039), and a lactate assay kit (MAK064), were obtained from Sigma.

    Article Title: Valproic Acid Induces Hair Regeneration in Murine Model and Activates Alkaline Phosphatase Activity in Human Dermal Papilla Cells
    Article Snippet: .. Cell Culture Human dermal papilla cells were obtained from Dr. Jin-Ho Jung in the Department of Dermatology at Seoul National University, and maintained in Dulbecco′s Modified Eagle Medium (DMEM) containing 10% (vol/vol) fetal bovine serum (FBS) and supplemented with 0.1 mg/ml G418 (Gibco-BRL). ..

    Transfection:

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
    Article Snippet: .. Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C. .. 5’-nested PCR primers (PDHB -P1~P6, ) and common 3’ primer (PDHB -R, ) were used to amplify six serial deletion fragments of the 5’-flanking region.

    Reporter Assay:

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
    Article Snippet: .. Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C. .. 5’-nested PCR primers (PDHB -P1~P6, ) and common 3’ primer (PDHB -R, ) were used to amplify six serial deletion fragments of the 5’-flanking region.

    Modification:

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene
    Article Snippet: .. Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C. .. 5’-nested PCR primers (PDHB -P1~P6, ) and common 3’ primer (PDHB -R, ) were used to amplify six serial deletion fragments of the 5’-flanking region.

    Article Title: Establishment of Human Papillomavirus Infection Requires Cell Cycle Progression
    Article Snippet: Plasmids pEF399, containing the complete W12E HPV16 genome , and all other plasmids used for virus packaging were previously described . pSEAP (pSEAP-control) for expression of secreted alkaline phosphatase (SEAP) was purchased from Clontech. pHPV16wpA-RL was cloned with HPV16 long control region (LCR, nucleotide position 7155 - 861), into pRL-null (Promega) to prepare a Renilla luciferase reporter system driven by native HPV16 promoter. .. Cell lines Human embryonic kidney cell line 293T from ATCC, and its enhanced SV40 T antigen-expressing daughter cell line 293TT from John Schiller, were maintained in Dulbecco's modified eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Invitrogen). .. Immortalized human keratinocyte cell line HaCaT was maintained in F-media (Invitrogen, 3 parts F-12 and 1 part DMEM), supplemented with 10% FBS.

    Article Title: Ex vivo Expansion of Bovine Corneal Endothelial Cells in Xeno-Free Medium Supplemented with Platelet Releasate
    Article Snippet: CECs were isolated by peeling from the corneal endothelium sheets and digested at 37°C in 1x TrypLE Express (Gibco, Life Technologies) for 30 min, as in our previous work . .. The medium used for isolation and culture was Supplemented Hormonal Epithelial Medium (SHEM), made of an equal volume of HEPES-buffered Dulbecco’s modified Eagle medium (DMEM) and Ham F12 (Invitrogen, Life Technologies) supplemented with 5% FBS (Gibco, Life Technologies), 5 µg/mL insulin, 5 µg/mL transferrin, 5 ng/mL selenium, 50 unit/mL penicillin, 50 µg/mL streptomycin, 250 ng/mL amphotericin B (all from Invitrogen, Life Technologies), 0.5% dimethyl sulfoxide (DMSO), 2 ng/mL recombinant human EGF (rHuEGF), and 1 nM cholera toxin (all from Sigma-Aldrich, St. Louis, MO, USA). ..

    Article Title: Valproic Acid Induces Hair Regeneration in Murine Model and Activates Alkaline Phosphatase Activity in Human Dermal Papilla Cells
    Article Snippet: .. Cell Culture Human dermal papilla cells were obtained from Dr. Jin-Ho Jung in the Department of Dermatology at Seoul National University, and maintained in Dulbecco′s Modified Eagle Medium (DMEM) containing 10% (vol/vol) fetal bovine serum (FBS) and supplemented with 0.1 mg/ml G418 (Gibco-BRL). ..

    Isolation:

    Article Title: Ex vivo Expansion of Bovine Corneal Endothelial Cells in Xeno-Free Medium Supplemented with Platelet Releasate
    Article Snippet: CECs were isolated by peeling from the corneal endothelium sheets and digested at 37°C in 1x TrypLE Express (Gibco, Life Technologies) for 30 min, as in our previous work . .. The medium used for isolation and culture was Supplemented Hormonal Epithelial Medium (SHEM), made of an equal volume of HEPES-buffered Dulbecco’s modified Eagle medium (DMEM) and Ham F12 (Invitrogen, Life Technologies) supplemented with 5% FBS (Gibco, Life Technologies), 5 µg/mL insulin, 5 µg/mL transferrin, 5 ng/mL selenium, 50 unit/mL penicillin, 50 µg/mL streptomycin, 250 ng/mL amphotericin B (all from Invitrogen, Life Technologies), 0.5% dimethyl sulfoxide (DMSO), 2 ng/mL recombinant human EGF (rHuEGF), and 1 nM cholera toxin (all from Sigma-Aldrich, St. Louis, MO, USA). ..

    Recombinant:

    Article Title: Ex vivo Expansion of Bovine Corneal Endothelial Cells in Xeno-Free Medium Supplemented with Platelet Releasate
    Article Snippet: CECs were isolated by peeling from the corneal endothelium sheets and digested at 37°C in 1x TrypLE Express (Gibco, Life Technologies) for 30 min, as in our previous work . .. The medium used for isolation and culture was Supplemented Hormonal Epithelial Medium (SHEM), made of an equal volume of HEPES-buffered Dulbecco’s modified Eagle medium (DMEM) and Ham F12 (Invitrogen, Life Technologies) supplemented with 5% FBS (Gibco, Life Technologies), 5 µg/mL insulin, 5 µg/mL transferrin, 5 ng/mL selenium, 50 unit/mL penicillin, 50 µg/mL streptomycin, 250 ng/mL amphotericin B (all from Invitrogen, Life Technologies), 0.5% dimethyl sulfoxide (DMSO), 2 ng/mL recombinant human EGF (rHuEGF), and 1 nM cholera toxin (all from Sigma-Aldrich, St. Louis, MO, USA). ..

    Chromatin Immunoprecipitation:

    Article Title: Ribosome Display Selection of a Murine IgG1 Fab Binding Affibody Molecule Allowing Species Selective Recovery Of Monoclonal Antibodies
    Article Snippet: C2–C3 (1 μM) was also injected over the target mIgG1 chip surfaces to confirm binding to mIgG1 . .. For analysis of Zmab25 or protein G C2–C3 domain interaction with fetal bovine serum (FBS), a third CM5 sensor chip was prepared onto which 488 RU of protein G C2–C3 and 1817 RU of Zmab25 were immobilized on separate surfaces, and 100 μl of 10% FBS (Gibco) or 10% FBS and 133 nM mAb3 were flowed over both surfaces. .. Sensor chip surfaces were regenerated with 10 μl 0.1 M glycine–HCl pH 2.0.

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  • 99
    Thermo Fisher fetal bovine serum fbs
    Cell cycle arrest by serum starvation abrogates HPV infection. (A) After 24 hr incubation in serum-free <t>DMEM,</t> HaCaT cells were inoculated with HPV pseudovirion hpv 16wpA-RL, and RL activity was measured after 2 d. For serum conversion, serum-free DMEM was replaced with 10% <t>FBS/DMEM</t> before virus inoculation. The expression value is shown as % infectivity to untreated cells with hpv 16wpA-RL inoculation. Columns , mean; bars , SD. (B) Flow cytometry was performed to confirm cell cycle arrest by serum starvation. The solid line indicates the HaCaT cells incubated with serum-free DMEM for 24 h, and the gray shaded area indicates control HaCaT cells in DMEM with 10% FBS.
    Fetal Bovine Serum Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal bovine serum fbs/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum fbs - by Bioz Stars, 2021-03
    99/100 stars
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    Cell cycle arrest by serum starvation abrogates HPV infection. (A) After 24 hr incubation in serum-free DMEM, HaCaT cells were inoculated with HPV pseudovirion hpv 16wpA-RL, and RL activity was measured after 2 d. For serum conversion, serum-free DMEM was replaced with 10% FBS/DMEM before virus inoculation. The expression value is shown as % infectivity to untreated cells with hpv 16wpA-RL inoculation. Columns , mean; bars , SD. (B) Flow cytometry was performed to confirm cell cycle arrest by serum starvation. The solid line indicates the HaCaT cells incubated with serum-free DMEM for 24 h, and the gray shaded area indicates control HaCaT cells in DMEM with 10% FBS.

    Journal: PLoS Pathogens

    Article Title: Establishment of Human Papillomavirus Infection Requires Cell Cycle Progression

    doi: 10.1371/journal.ppat.1000318

    Figure Lengend Snippet: Cell cycle arrest by serum starvation abrogates HPV infection. (A) After 24 hr incubation in serum-free DMEM, HaCaT cells were inoculated with HPV pseudovirion hpv 16wpA-RL, and RL activity was measured after 2 d. For serum conversion, serum-free DMEM was replaced with 10% FBS/DMEM before virus inoculation. The expression value is shown as % infectivity to untreated cells with hpv 16wpA-RL inoculation. Columns , mean; bars , SD. (B) Flow cytometry was performed to confirm cell cycle arrest by serum starvation. The solid line indicates the HaCaT cells incubated with serum-free DMEM for 24 h, and the gray shaded area indicates control HaCaT cells in DMEM with 10% FBS.

    Article Snippet: Cell lines Human embryonic kidney cell line 293T from ATCC, and its enhanced SV40 T antigen-expressing daughter cell line 293TT from John Schiller, were maintained in Dulbecco's modified eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Invitrogen).

    Techniques: Infection, Incubation, Activity Assay, Expressing, Flow Cytometry, Cytometry

    Cell cycle arrest does not inhibit influenza virus. (A) After 24 hr synchronization with aphidicolin and 4 hr treatment with etoposide or aphidicolin (3 µM each), 293T cells were inoculated in parallel with hpv 16wpA-RL or an influenza virus vector in which the hemagglutinin and neuraminidase open reading frames in viral RNA were replaced with those of vesicular stomatitis virus glycoprotein and RL, respectively [50] . (B) After 24 hr synchronization in serum-free DMEM, HaCaT cells were inoculated with hpv 16wpA-RL and the RL-expressing influenza virus as (A) in the presence or absence of FBS. RL activity was measured after 48 hrs, as described in Materials and Methods , normalized to RL activity in equivalently inoculated cells maintained in 10% FBS, and expressed in the histograms as % infectivity. Columns , mean; bars , SD.

    Journal: PLoS Pathogens

    Article Title: Establishment of Human Papillomavirus Infection Requires Cell Cycle Progression

    doi: 10.1371/journal.ppat.1000318

    Figure Lengend Snippet: Cell cycle arrest does not inhibit influenza virus. (A) After 24 hr synchronization with aphidicolin and 4 hr treatment with etoposide or aphidicolin (3 µM each), 293T cells were inoculated in parallel with hpv 16wpA-RL or an influenza virus vector in which the hemagglutinin and neuraminidase open reading frames in viral RNA were replaced with those of vesicular stomatitis virus glycoprotein and RL, respectively [50] . (B) After 24 hr synchronization in serum-free DMEM, HaCaT cells were inoculated with hpv 16wpA-RL and the RL-expressing influenza virus as (A) in the presence or absence of FBS. RL activity was measured after 48 hrs, as described in Materials and Methods , normalized to RL activity in equivalently inoculated cells maintained in 10% FBS, and expressed in the histograms as % infectivity. Columns , mean; bars , SD.

    Article Snippet: Cell lines Human embryonic kidney cell line 293T from ATCC, and its enhanced SV40 T antigen-expressing daughter cell line 293TT from John Schiller, were maintained in Dulbecco's modified eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Invitrogen).

    Techniques: Plasmid Preparation, Expressing, Activity Assay, Infection

    Chondrogenic differentiation of hiPSC. ( a ) Classical chondrogenic differentiation of hiPSCs via formation of embryoid bodies, outgrowth of endodermal ( green ), ectodermal ( yellow ) and mesodermal ( red ) cell lineages, selection of mesodermal cells, and induction of MSC and induction of chondrocytes. In this method hiPS cells were detached from matrigel coated dish and moved to ultra low attachment culture dish for 5 days to induce the EB formation, then EBs moved to plastic culture dish to select the hMSCs by collecting the outgrowing cells from EB (from day 5 to day 14) after collecting the attached fibroblast-like cells. These cells were cultured for 3 weeks in media containing FBS to prepare the hiPSC-MSCs (day 35 of differentiation). Then, hiPSC-MSCs were differentiated in a pellet culture system using serum free chondrogenic media for 3 weeks. ( b ) Embryoid body free method of direct differentiation of hiPSCs into hiPSC-MSCs, followed by chondrogenic differentiation. In embryoid body free method hiPSCs were cultured in matrigel coated dish and media was changed to hMSC media (DMEM supplemented with FBS) for 5 days to induce the hMSC differentiation (Day 5). Then, cells were detached and moved to a plastic culture dish for 4 passages to prepare the hiPSC-MSCs (Day 28). To differentiate the hiPSC-MSCs to chondrocytes, cells were used in pellet culture system using serum free chondrogenic media for 3 weeks

    Journal: Stem Cell Reviews

    Article Title: Improved Approach for Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells

    doi: 10.1007/s12015-014-9581-5

    Figure Lengend Snippet: Chondrogenic differentiation of hiPSC. ( a ) Classical chondrogenic differentiation of hiPSCs via formation of embryoid bodies, outgrowth of endodermal ( green ), ectodermal ( yellow ) and mesodermal ( red ) cell lineages, selection of mesodermal cells, and induction of MSC and induction of chondrocytes. In this method hiPS cells were detached from matrigel coated dish and moved to ultra low attachment culture dish for 5 days to induce the EB formation, then EBs moved to plastic culture dish to select the hMSCs by collecting the outgrowing cells from EB (from day 5 to day 14) after collecting the attached fibroblast-like cells. These cells were cultured for 3 weeks in media containing FBS to prepare the hiPSC-MSCs (day 35 of differentiation). Then, hiPSC-MSCs were differentiated in a pellet culture system using serum free chondrogenic media for 3 weeks. ( b ) Embryoid body free method of direct differentiation of hiPSCs into hiPSC-MSCs, followed by chondrogenic differentiation. In embryoid body free method hiPSCs were cultured in matrigel coated dish and media was changed to hMSC media (DMEM supplemented with FBS) for 5 days to induce the hMSC differentiation (Day 5). Then, cells were detached and moved to a plastic culture dish for 4 passages to prepare the hiPSC-MSCs (Day 28). To differentiate the hiPSC-MSCs to chondrocytes, cells were used in pellet culture system using serum free chondrogenic media for 3 weeks

    Article Snippet: Osteogenic differentiation was induced by culturing 6 × 104 cells/cm2 in osteogenic differentiation medium consisting of DMEM supplemented by 10 % FBS (Gibco), 100 U/ml penicillin, 100 μg/ml streptomycin (Gibco), 10 % L-Glutamine (Gibco), 50 μg/ml L-ascorbic acid 2-phosphate sequimagnesium (Sigma), 100 μg/ml MEM sodium pyruvate (Gibco), 0.1 μM dexamethasone (Sigma), and 100 mM b-glycerophosphate.

    Techniques: Selection, Cell Culture

    Promoter activity analysis of the bovine PDHB gene. a. We transferred six serial deletion constructs in pGL3-basic into C2C12 cells. After 5 h we replaced the transfection mixture with DMEM with 5% FBS (myoblasts) or 2% HS (myotubes). b. We transferred the same constructs into 3T3-L1 cells. We normalized relative luciferase activities to Renilla luciferase activity. The transcription factor binding sites of MYOG and C/EBPß are indicated with closed circles and ellipses, respectively. *, P

    Journal: PLoS ONE

    Article Title: Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene

    doi: 10.1371/journal.pone.0157445

    Figure Lengend Snippet: Promoter activity analysis of the bovine PDHB gene. a. We transferred six serial deletion constructs in pGL3-basic into C2C12 cells. After 5 h we replaced the transfection mixture with DMEM with 5% FBS (myoblasts) or 2% HS (myotubes). b. We transferred the same constructs into 3T3-L1 cells. We normalized relative luciferase activities to Renilla luciferase activity. The transcription factor binding sites of MYOG and C/EBPß are indicated with closed circles and ellipses, respectively. *, P

    Article Snippet: Cell culture, transfection and dual-luciferase reporter assay We cultured the mouse myoblast cell line (C2C12) and the 3T3-L1 cell line in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (GIBCO-Invitrogen) under humidified air containing 5% CO2 at 37°C.

    Techniques: Activity Assay, Construct, Transfection, Luciferase, Binding Assay

    Binding studies of Z mab25 or protein G with fetal bovine serum and species selective affinity recovery of two monoclonal antibodies. a 10% FBS was flowed over a Z mab25 ( gray solid trace ) or protein G C2–C3 fragment (SPG) ( black dashed trace ) BIAcore biosensor surface. For comparison, a sample containing 10% FBS spiked with mouse IgG 1 mAb3 was also flowed over the Z mab25 surface ( black solid trace ). b : SDS-PAGE analysis (reducing conditions) of samples, flow-through (FT), and eluate (E) fractions from affinity chromatography experiments using either a protein G (SPG) column or a Z mab25 column, and samples of 10% FBS only ( lanes 1 – 6 ) or 10% FBS with in-spiked mouse IgG 1 mAb3 ( lanes 7 – 12 ) or 10% FBS with in-spiked mouse IgG 1 8F11 ( lanes 13 – 15 ). M LMW-SDS molecular weight marker; lane 1 : 10% FBS sample; lane 2 : SPG column, FT; lane 3 : SPG column, E; lane 4 : 10% FBS sample; lane 5 : Z mab25 column, FT; lane 6 : Z mab25 column, E; lane 7 : 10% FBS + mAb3 sample; lane 8 : SPG column, FT; lane 9 : SPG column, E; lane 10 : 10% FBS + mAb3 sample; lane 11 : Z mab25 column, FT; lane 12 : Z mab25 column, E; lane 13 : 10% FBS + mAb 8F11 sample; lane 14 : Z mab25 column, FT; lane 15 : Z mab25 column, E. The black arrows to the right indicate nominal molecular weights of antibody heavy and light chains, respectively. The numbers to the left indicate molecular weights in kilo Dalton (kDa). c Results from a biosensor analysis of pH neutralized eluates from the SPG and Z mab25 columns, originating from the use of the 10% FBS sample containing in-spiked mAb3 ( 1 – 4 ) or mAb 8F11 ( 5 , 6 ). On separate flow cell surfaces, anti-cow IgG and anti-mouse Ig antibodies were immobilized, and samples were injected. (1) black solid trace SPG column eluate, anti-cow IgG surface; (2) black dashed trace SPG column eluate, anti-mouse Ig surface; (3) gray solid trace Z mab25 column eluate, anti-cow IgG surface; (4) gray dashed trace Z mab25 column eluate, anti-mouse Ig surface; (5) black dotted trace Z mab25 column eluate, anti-cow IgG surface; (6) gray dotted trace Z mab25 column eluate, anti-mouse Ig surface

    Journal: Molecular Biotechnology

    Article Title: Ribosome Display Selection of a Murine IgG1 Fab Binding Affibody Molecule Allowing Species Selective Recovery Of Monoclonal Antibodies

    doi: 10.1007/s12033-010-9367-1

    Figure Lengend Snippet: Binding studies of Z mab25 or protein G with fetal bovine serum and species selective affinity recovery of two monoclonal antibodies. a 10% FBS was flowed over a Z mab25 ( gray solid trace ) or protein G C2–C3 fragment (SPG) ( black dashed trace ) BIAcore biosensor surface. For comparison, a sample containing 10% FBS spiked with mouse IgG 1 mAb3 was also flowed over the Z mab25 surface ( black solid trace ). b : SDS-PAGE analysis (reducing conditions) of samples, flow-through (FT), and eluate (E) fractions from affinity chromatography experiments using either a protein G (SPG) column or a Z mab25 column, and samples of 10% FBS only ( lanes 1 – 6 ) or 10% FBS with in-spiked mouse IgG 1 mAb3 ( lanes 7 – 12 ) or 10% FBS with in-spiked mouse IgG 1 8F11 ( lanes 13 – 15 ). M LMW-SDS molecular weight marker; lane 1 : 10% FBS sample; lane 2 : SPG column, FT; lane 3 : SPG column, E; lane 4 : 10% FBS sample; lane 5 : Z mab25 column, FT; lane 6 : Z mab25 column, E; lane 7 : 10% FBS + mAb3 sample; lane 8 : SPG column, FT; lane 9 : SPG column, E; lane 10 : 10% FBS + mAb3 sample; lane 11 : Z mab25 column, FT; lane 12 : Z mab25 column, E; lane 13 : 10% FBS + mAb 8F11 sample; lane 14 : Z mab25 column, FT; lane 15 : Z mab25 column, E. The black arrows to the right indicate nominal molecular weights of antibody heavy and light chains, respectively. The numbers to the left indicate molecular weights in kilo Dalton (kDa). c Results from a biosensor analysis of pH neutralized eluates from the SPG and Z mab25 columns, originating from the use of the 10% FBS sample containing in-spiked mAb3 ( 1 – 4 ) or mAb 8F11 ( 5 , 6 ). On separate flow cell surfaces, anti-cow IgG and anti-mouse Ig antibodies were immobilized, and samples were injected. (1) black solid trace SPG column eluate, anti-cow IgG surface; (2) black dashed trace SPG column eluate, anti-mouse Ig surface; (3) gray solid trace Z mab25 column eluate, anti-cow IgG surface; (4) gray dashed trace Z mab25 column eluate, anti-mouse Ig surface; (5) black dotted trace Z mab25 column eluate, anti-cow IgG surface; (6) gray dotted trace Z mab25 column eluate, anti-mouse Ig surface

    Article Snippet: For analysis of Zmab25 or protein G C2–C3 domain interaction with fetal bovine serum (FBS), a third CM5 sensor chip was prepared onto which 488 RU of protein G C2–C3 and 1817 RU of Zmab25 were immobilized on separate surfaces, and 100 μl of 10% FBS (Gibco) or 10% FBS and 133 nM mAb3 were flowed over both surfaces.

    Techniques: Binding Assay, SDS Page, Flow Cytometry, Affinity Chromatography, Molecular Weight, Marker, Injection