fetal bovine serum  (ATCC)


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    Name:
    Fetal Bovine Serum FBS
    Description:
    Triple filtered through 0 1 μm filters Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States
    Catalog Number:
    30-2021
    Price:
    None
    Applications:
    Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.
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    ATCC fetal bovine serum
    Triple filtered through 0 1 μm filters Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States
    https://www.bioz.com/result/fetal bovine serum/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions
    Article Snippet: Bacterial strains, media and culture conditions Smooth virulent Brucella melitensis 16 M Biotype 1 (ATCC 23456) (American Type Culture Collection, Manassas, VA), re-isolated from an aborted goat fetus, and its derivatives were maintained as frozen glycerol stocks. .. Individual 50 ml conical tubes were filled with 10 ml of cell culture medium [F12K medium (ATCC® ) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (ATCC® )], inoculated with 0.1 ml (1:100 for mid-log cultures), 0.25 ml (1:40 for late-log phase cultures) and 1 ml (1:10 for stationary phase cultures) of a saturated culture of B. melitensis 16 M and incubated overnight at 37°C with 5% CO2 , loose lids and shaking (200 rpm). ..

    Article Title: Beta 2 adrenergic receptor agonists are novel regulators of macrophage activation in diabetic renal and cardiovascular complications
    Article Snippet: BMMs were prepared by flushing the dissected femur and tibia of 8–12-week-old Sprague-Dawley (SD) rats (Charles River Laboratories (CRL), Wilmington, MA). .. Cells were cultured in RPMI containing 10% fetal bovine serum (FBS) and 20% L929 cell (American Type Culture Collection [ATCC], Manassas, VA) conditioned media as a source of macrophage colony stimulating factor . .. After 7 days, at least 97% of the cells were double positive for CD68 (Serotec, Oxford, UK) and CD11b/c (BD Biosciences, San Diego, CA).

    Article Title: FOXM1 is a downstream target of LPA and YAP oncogenic signaling pathways in high grade serous ovarian cancer
    Article Snippet: OVCA433 cells were cultured in RPMI 1640 with glutamine, 10% FBS (ATCC, Manassas, VA), and 100 μg/mL penicillin/streptomycin (P/S). .. CAOV3 were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), and 100 μg/mL P/S. .. OVCAR5 cells were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), 0.1 mM MEM Non-Essential Amino Acids (NEAA), 2 mM L-glutamine and 100 μg/mL P/S.

    Article Title: FOXM1 is a downstream target of LPA and YAP oncogenic signaling pathways in high grade serous ovarian cancer
    Article Snippet: CAOV3 were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), and 100 μg/mL P/S. .. OVCAR5 cells were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), 0.1 mM MEM Non-Essential Amino Acids (NEAA), 2 mM L-glutamine and 100 μg/mL P/S. .. For serum starvation, cells were incubated in the basal medium without FBS or antibiotics.

    Incubation:

    Article Title: Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions
    Article Snippet: Bacterial strains, media and culture conditions Smooth virulent Brucella melitensis 16 M Biotype 1 (ATCC 23456) (American Type Culture Collection, Manassas, VA), re-isolated from an aborted goat fetus, and its derivatives were maintained as frozen glycerol stocks. .. Individual 50 ml conical tubes were filled with 10 ml of cell culture medium [F12K medium (ATCC® ) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (ATCC® )], inoculated with 0.1 ml (1:100 for mid-log cultures), 0.25 ml (1:40 for late-log phase cultures) and 1 ml (1:10 for stationary phase cultures) of a saturated culture of B. melitensis 16 M and incubated overnight at 37°C with 5% CO2 , loose lids and shaking (200 rpm). ..

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  • bxpc 3  (ATCC)
    99
    ATCC bxpc 3
    Phospho-drug loaded-CPSNPs block pancreatic cancer cell proliferation In vitro growth of human PDAC cell lines <t>BxPC-3</t> and PANC-1 is effectively blocked by mPEG-dFdCMPs, with EC 50 values of 130 and 550 nM, respectively. BxPC-3 cells were more resistant to mPEG-FdUMP-CPSNPS than PANC-1 cells, which had an EC 50 of 1.3 μM. Empty mPEG-CPSNPs (light hatched), free drug (black) or drug-containing CPSNPs (shaded hatched) are expressed as relative proliferation (percent of vehicle, white). Values are the mean of 3–4 independent experiments; *** p
    Bxpc 3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bxpc 3/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bxpc 3 - by Bioz Stars, 2021-03
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    97
    ATCC bovine serum fbs
    Flow-charts showing the preparation of RIN-5F cells and their survival rate evaluation. A. Preparation of RIN-5F cells before starting 1–5-day preservation experiments at +4°C. B. Procedures to estimate the number of living cells using the hemocytometer. C. Procedures to measure the amount of insulin secreted from the living cells. Medium-A consists of <t>RPMI-1640</t> medium containing 10% of fetal bovine serum <t>(FBS).</t> Medium-B consists of the medium containing 1% of FBS. Trypsin-A consists of 0.25% bovine trypsin plus 1 mM of EDTA dissolved in phosphate buffered saline (PBS).
    Bovine Serum Fbs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
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    Image Search Results


    Phospho-drug loaded-CPSNPs block pancreatic cancer cell proliferation In vitro growth of human PDAC cell lines BxPC-3 and PANC-1 is effectively blocked by mPEG-dFdCMPs, with EC 50 values of 130 and 550 nM, respectively. BxPC-3 cells were more resistant to mPEG-FdUMP-CPSNPS than PANC-1 cells, which had an EC 50 of 1.3 μM. Empty mPEG-CPSNPs (light hatched), free drug (black) or drug-containing CPSNPs (shaded hatched) are expressed as relative proliferation (percent of vehicle, white). Values are the mean of 3–4 independent experiments; *** p

    Journal: Nanomedicine : nanotechnology, biology, and medicine

    Article Title: Effective Encapsulation and Biological Activity of Phosphorylated Chemotherapeutics in Calcium Phosphosilicate Nanoparticles for the Treatment of Pancreatic Cancer

    doi: 10.1016/j.nano.2017.06.017

    Figure Lengend Snippet: Phospho-drug loaded-CPSNPs block pancreatic cancer cell proliferation In vitro growth of human PDAC cell lines BxPC-3 and PANC-1 is effectively blocked by mPEG-dFdCMPs, with EC 50 values of 130 and 550 nM, respectively. BxPC-3 cells were more resistant to mPEG-FdUMP-CPSNPS than PANC-1 cells, which had an EC 50 of 1.3 μM. Empty mPEG-CPSNPs (light hatched), free drug (black) or drug-containing CPSNPs (shaded hatched) are expressed as relative proliferation (percent of vehicle, white). Values are the mean of 3–4 independent experiments; *** p

    Article Snippet: PANC-1 (in Dulbecco’s modified Eagle medium/10% fetal bovine serum) and BxPC-3 (in RPMI medium/10% fetal bovine serum) cells (ATCC) were seeded onto 96-well plates at 5,000 cells/well.

    Techniques: Blocking Assay, In Vitro

    CPSNP-encapsulated FdUMP inactivates TS Immunoblots of the FdUMP target enzyme thymidylate synthase (TS) from PANC-1 (upper panel) or BxPC-3 (lower panel) cells treated with (Lane 3) 250 μM free FdUMP or (Lane 5) 2 μM mPEG-FdUMP-CPSNPs. Both cell lines showed significant ( > 80%) conversion of TS to an inactive ternary complex (TS:FdUMP) with free drug and with mPEG-FdUMP-CPSNP treatment. Controls that received (Lane 1) no treatment, (Lane 2) vehicle or (Lane 4) mPEG-CPSNPs exhibited only active TS with no evidence of TS:FdUMP ternary complex formation.

    Journal: Nanomedicine : nanotechnology, biology, and medicine

    Article Title: Effective Encapsulation and Biological Activity of Phosphorylated Chemotherapeutics in Calcium Phosphosilicate Nanoparticles for the Treatment of Pancreatic Cancer

    doi: 10.1016/j.nano.2017.06.017

    Figure Lengend Snippet: CPSNP-encapsulated FdUMP inactivates TS Immunoblots of the FdUMP target enzyme thymidylate synthase (TS) from PANC-1 (upper panel) or BxPC-3 (lower panel) cells treated with (Lane 3) 250 μM free FdUMP or (Lane 5) 2 μM mPEG-FdUMP-CPSNPs. Both cell lines showed significant ( > 80%) conversion of TS to an inactive ternary complex (TS:FdUMP) with free drug and with mPEG-FdUMP-CPSNP treatment. Controls that received (Lane 1) no treatment, (Lane 2) vehicle or (Lane 4) mPEG-CPSNPs exhibited only active TS with no evidence of TS:FdUMP ternary complex formation.

    Article Snippet: PANC-1 (in Dulbecco’s modified Eagle medium/10% fetal bovine serum) and BxPC-3 (in RPMI medium/10% fetal bovine serum) cells (ATCC) were seeded onto 96-well plates at 5,000 cells/well.

    Techniques: Western Blot

    Flow-charts showing the preparation of RIN-5F cells and their survival rate evaluation. A. Preparation of RIN-5F cells before starting 1–5-day preservation experiments at +4°C. B. Procedures to estimate the number of living cells using the hemocytometer. C. Procedures to measure the amount of insulin secreted from the living cells. Medium-A consists of RPMI-1640 medium containing 10% of fetal bovine serum (FBS). Medium-B consists of the medium containing 1% of FBS. Trypsin-A consists of 0.25% bovine trypsin plus 1 mM of EDTA dissolved in phosphate buffered saline (PBS).

    Journal: PLoS ONE

    Article Title: Antifreeze Protein Prolongs the Life-Time of Insulinoma Cells during Hypothermic Preservation

    doi: 10.1371/journal.pone.0073643

    Figure Lengend Snippet: Flow-charts showing the preparation of RIN-5F cells and their survival rate evaluation. A. Preparation of RIN-5F cells before starting 1–5-day preservation experiments at +4°C. B. Procedures to estimate the number of living cells using the hemocytometer. C. Procedures to measure the amount of insulin secreted from the living cells. Medium-A consists of RPMI-1640 medium containing 10% of fetal bovine serum (FBS). Medium-B consists of the medium containing 1% of FBS. Trypsin-A consists of 0.25% bovine trypsin plus 1 mM of EDTA dissolved in phosphate buffered saline (PBS).

    Article Snippet: Their components are as follows: medium A, RPMI-1640 medium (ATCC, 30-2001) containing 10% of fetal bovine serum (FBS) (ATCC, 30-2020); medium B: the RPMI-1640 medium containing 1% of FBS; trypsin A: 0.25% bovine trypsin plus 1 mM of EDTA dissolved in phosphate buffered saline (PBS, 0.2 g/l of KCl, 0.2 g/l of KH2 PO4 , 8 g/l of NaCl, and 2.9 g/l of Na2 HPO4 -12H2 O).

    Techniques: Flow Cytometry, Preserving

    The ability of B. melitensis 16 M at different phases of growth to invade HeLa cells . (A) Growth curve of B. melitensis 16 M grown overnight in tubes with loose lids and shaking in F12K cell culture medium supplemented with 10% (v/v) HI-FBS. Results are the average +/- SD of 3 independent experiments. Mid-log, late-log and stationary growth phases are marked with *. (B) HeLa cell infections were performed at MOI 1,000:1 for 30 min. The intracellular number of late-log growth phase cultures of B. melitensis was significantly different from those grown to mid-log (* = P

    Journal: BMC Microbiology

    Article Title: Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions

    doi: 10.1186/1471-2180-9-81

    Figure Lengend Snippet: The ability of B. melitensis 16 M at different phases of growth to invade HeLa cells . (A) Growth curve of B. melitensis 16 M grown overnight in tubes with loose lids and shaking in F12K cell culture medium supplemented with 10% (v/v) HI-FBS. Results are the average +/- SD of 3 independent experiments. Mid-log, late-log and stationary growth phases are marked with *. (B) HeLa cell infections were performed at MOI 1,000:1 for 30 min. The intracellular number of late-log growth phase cultures of B. melitensis was significantly different from those grown to mid-log (* = P

    Article Snippet: Individual 50 ml conical tubes were filled with 10 ml of cell culture medium [F12K medium (ATCC® ) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (ATCC® )], inoculated with 0.1 ml (1:100 for mid-log cultures), 0.25 ml (1:40 for late-log phase cultures) and 1 ml (1:10 for stationary phase cultures) of a saturated culture of B. melitensis 16 M and incubated overnight at 37°C with 5% CO2 , loose lids and shaking (200 rpm).

    Techniques: Cell Culture

    LPA-induced FOXM1 in other EOC cell lines and FOXM1C was the dominant form in CAOV3 cells A. LPA (10 μM and 6 hr) induced FOXM1 up-regulation in CAOV3 and OVCAR5 cells. CAOV3 and OVCAR5 cells expressed the FOXM1B with apparent MW of 80 KDa (Sigma, Cat. Log # AV39518). B. OVCA433 cells did not express FOXM1C, while CAOV3 cells expressed the FOXM1C with apparent MW of 100 KDa (Cell Signaling, Cat. Log # D12D5). C. CAOV3-FOXM1-KD cell line was established using shRNA against FOXM1 (detailed in Methods). The parental or control-shRNA transfected CAOV3 cells responded to LPA (10 μM, 6 hr) for FOXM1 up-regulation, which was blocked by KD-FOXM1. D. The dominant splicing form of FOXM1 in CAOV3 was FOXM1C. E. Comparison of the relative mRNA expression levels FOXM1 isoform in three EOC cell lines. F. KD-YAP and PTX inhibited LPA-induced FOXM1 up-regulation in CAOV3 cells. G. KD-FOXM1 inhibited LPA-induced cell migration and invasion. H. KD-FOXM1 inhibited FBS (2%)-induced cell proliferation. * P

    Journal: Oncotarget

    Article Title: FOXM1 is a downstream target of LPA and YAP oncogenic signaling pathways in high grade serous ovarian cancer

    doi:

    Figure Lengend Snippet: LPA-induced FOXM1 in other EOC cell lines and FOXM1C was the dominant form in CAOV3 cells A. LPA (10 μM and 6 hr) induced FOXM1 up-regulation in CAOV3 and OVCAR5 cells. CAOV3 and OVCAR5 cells expressed the FOXM1B with apparent MW of 80 KDa (Sigma, Cat. Log # AV39518). B. OVCA433 cells did not express FOXM1C, while CAOV3 cells expressed the FOXM1C with apparent MW of 100 KDa (Cell Signaling, Cat. Log # D12D5). C. CAOV3-FOXM1-KD cell line was established using shRNA against FOXM1 (detailed in Methods). The parental or control-shRNA transfected CAOV3 cells responded to LPA (10 μM, 6 hr) for FOXM1 up-regulation, which was blocked by KD-FOXM1. D. The dominant splicing form of FOXM1 in CAOV3 was FOXM1C. E. Comparison of the relative mRNA expression levels FOXM1 isoform in three EOC cell lines. F. KD-YAP and PTX inhibited LPA-induced FOXM1 up-regulation in CAOV3 cells. G. KD-FOXM1 inhibited LPA-induced cell migration and invasion. H. KD-FOXM1 inhibited FBS (2%)-induced cell proliferation. * P

    Article Snippet: CAOV3 were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), and 100 μg/mL P/S.

    Techniques: shRNA, Transfection, Expressing, Migration