fetal bovine serum  (ATCC)

Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Effective Encapsulation and Biological Activity of Phosphorylated Chemotherapeutics in Calcium Phosphosilicate Nanoparticles for the Treatment of Pancreatic Cancer

doi: 10.1016/j.nano.2017.06.017

Figure Lengend Snippet: Phospho-drug loaded-CPSNPs block pancreatic cancer cell proliferation In vitro growth of human PDAC cell lines BxPC-3 and PANC-1 is effectively blocked by mPEG-dFdCMPs, with EC 50 values of 130 and 550 nM, respectively. BxPC-3 cells were more resistant to mPEG-FdUMP-CPSNPS than PANC-1 cells, which had an EC 50 of 1.3 μM. Empty mPEG-CPSNPs (light hatched), free drug (black) or drug-containing CPSNPs (shaded hatched) are expressed as relative proliferation (percent of vehicle, white). Values are the mean of 3–4 independent experiments; *** p

Article Snippet: PANC-1 (in Dulbecco’s modified Eagle medium/10% fetal bovine serum) and BxPC-3 (in RPMI medium/10% fetal bovine serum) cells (ATCC) were seeded onto 96-well plates at 5,000 cells/well.

Techniques: Blocking Assay, In Vitro

Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Effective Encapsulation and Biological Activity of Phosphorylated Chemotherapeutics in Calcium Phosphosilicate Nanoparticles for the Treatment of Pancreatic Cancer

doi: 10.1016/j.nano.2017.06.017

Figure Lengend Snippet: CPSNP-encapsulated FdUMP inactivates TS Immunoblots of the FdUMP target enzyme thymidylate synthase (TS) from PANC-1 (upper panel) or BxPC-3 (lower panel) cells treated with (Lane 3) 250 μM free FdUMP or (Lane 5) 2 μM mPEG-FdUMP-CPSNPs. Both cell lines showed significant ( > 80%) conversion of TS to an inactive ternary complex (TS:FdUMP) with free drug and with mPEG-FdUMP-CPSNP treatment. Controls that received (Lane 1) no treatment, (Lane 2) vehicle or (Lane 4) mPEG-CPSNPs exhibited only active TS with no evidence of TS:FdUMP ternary complex formation.

Article Snippet: PANC-1 (in Dulbecco’s modified Eagle medium/10% fetal bovine serum) and BxPC-3 (in RPMI medium/10% fetal bovine serum) cells (ATCC) were seeded onto 96-well plates at 5,000 cells/well.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Antifreeze Protein Prolongs the Life-Time of Insulinoma Cells during Hypothermic Preservation

doi: 10.1371/journal.pone.0073643

Figure Lengend Snippet: Flow-charts showing the preparation of RIN-5F cells and their survival rate evaluation. A. Preparation of RIN-5F cells before starting 1–5-day preservation experiments at +4°C. B. Procedures to estimate the number of living cells using the hemocytometer. C. Procedures to measure the amount of insulin secreted from the living cells. Medium-A consists of RPMI-1640 medium containing 10% of fetal bovine serum (FBS). Medium-B consists of the medium containing 1% of FBS. Trypsin-A consists of 0.25% bovine trypsin plus 1 mM of EDTA dissolved in phosphate buffered saline (PBS).

Article Snippet: Their components are as follows: medium A, RPMI-1640 medium (ATCC, 30-2001) containing 10% of fetal bovine serum (FBS) (ATCC, 30-2020); medium B: the RPMI-1640 medium containing 1% of FBS; trypsin A: 0.25% bovine trypsin plus 1 mM of EDTA dissolved in phosphate buffered saline (PBS, 0.2 g/l of KCl, 0.2 g/l of KH2 PO4 , 8 g/l of NaCl, and 2.9 g/l of Na2 HPO4 -12H2 O).

Techniques: Flow Cytometry, Preserving

Journal: BMC Microbiology

Article Title: Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions

doi: 10.1186/1471-2180-9-81

Figure Lengend Snippet: The ability of B. melitensis 16 M at different phases of growth to invade HeLa cells . (A) Growth curve of B. melitensis 16 M grown overnight in tubes with loose lids and shaking in F12K cell culture medium supplemented with 10% (v/v) HI-FBS. Results are the average +/- SD of 3 independent experiments. Mid-log, late-log and stationary growth phases are marked with *. (B) HeLa cell infections were performed at MOI 1,000:1 for 30 min. The intracellular number of late-log growth phase cultures of B. melitensis was significantly different from those grown to mid-log (* = P

Article Snippet: Individual 50 ml conical tubes were filled with 10 ml of cell culture medium [F12K medium (ATCC® ) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (ATCC® )], inoculated with 0.1 ml (1:100 for mid-log cultures), 0.25 ml (1:40 for late-log phase cultures) and 1 ml (1:10 for stationary phase cultures) of a saturated culture of B. melitensis 16 M and incubated overnight at 37°C with 5% CO2 , loose lids and shaking (200 rpm).

Techniques: Cell Culture

Journal: Oncotarget

Article Title: FOXM1 is a downstream target of LPA and YAP oncogenic signaling pathways in high grade serous ovarian cancer

doi:

Figure Lengend Snippet: LPA-induced FOXM1 in other EOC cell lines and FOXM1C was the dominant form in CAOV3 cells A. LPA (10 μM and 6 hr) induced FOXM1 up-regulation in CAOV3 and OVCAR5 cells. CAOV3 and OVCAR5 cells expressed the FOXM1B with apparent MW of 80 KDa (Sigma, Cat. Log # AV39518). B. OVCA433 cells did not express FOXM1C, while CAOV3 cells expressed the FOXM1C with apparent MW of 100 KDa (Cell Signaling, Cat. Log # D12D5). C. CAOV3-FOXM1-KD cell line was established using shRNA against FOXM1 (detailed in Methods). The parental or control-shRNA transfected CAOV3 cells responded to LPA (10 μM, 6 hr) for FOXM1 up-regulation, which was blocked by KD-FOXM1. D. The dominant splicing form of FOXM1 in CAOV3 was FOXM1C. E. Comparison of the relative mRNA expression levels FOXM1 isoform in three EOC cell lines. F. KD-YAP and PTX inhibited LPA-induced FOXM1 up-regulation in CAOV3 cells. G. KD-FOXM1 inhibited LPA-induced cell migration and invasion. H. KD-FOXM1 inhibited FBS (2%)-induced cell proliferation. * P

Article Snippet: CAOV3 were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), and 100 μg/mL P/S.

Techniques: shRNA, Transfection, Expressing, Migration