Structured Review

ScienCell fetal bovine serum fbs
Puerarin inhibited TGF-β1-induced HUVECs migration rate . HUVECs were preincubated with different concentrations of puerarin (10, 25, and 50 μ M) for 30 min and then treated with TGF- β 1 (10 ng/ml) for 48 h until a monolayer was formed. Scratches were made straightly across the dishes. After rinsing twice, the medium was replaced by <t>RPMI1640</t> with no <t>FBS.</t> Photographs were taken at indicated times (scale bars: 50 μ m).
Fetal Bovine Serum Fbs, supplied by ScienCell, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 12 article reviews
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fetal bovine serum fbs - by Bioz Stars, 2020-07
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Images

1) Product Images from "Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition"

Article Title: Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition

Journal: PPAR Research

doi: 10.1155/2017/2647129

Puerarin inhibited TGF-β1-induced HUVECs migration rate . HUVECs were preincubated with different concentrations of puerarin (10, 25, and 50 μ M) for 30 min and then treated with TGF- β 1 (10 ng/ml) for 48 h until a monolayer was formed. Scratches were made straightly across the dishes. After rinsing twice, the medium was replaced by RPMI1640 with no FBS. Photographs were taken at indicated times (scale bars: 50 μ m).
Figure Legend Snippet: Puerarin inhibited TGF-β1-induced HUVECs migration rate . HUVECs were preincubated with different concentrations of puerarin (10, 25, and 50 μ M) for 30 min and then treated with TGF- β 1 (10 ng/ml) for 48 h until a monolayer was formed. Scratches were made straightly across the dishes. After rinsing twice, the medium was replaced by RPMI1640 with no FBS. Photographs were taken at indicated times (scale bars: 50 μ m).

Techniques Used: Migration

Related Articles

Transfection:

Article Title: NFAT5 promotes arteriogenesis via MCP‐1‐dependent monocyte recruitment, et al. NFAT5 promotes arteriogenesis via MCP‐1‐dependent monocyte recruitment
Article Snippet: .. 2.5 Cell culture and transfection Human umbilical vein endothelial cells were purchased from ScienCell Research Laboratories, Inc and maintained in endothelial cell medium (ScienCell) supplemented with 5% foetal bovine serum (FBS), endothelial cell growth supplement and 1% penicillin/streptomycin (ScienCell). .. THP‐1 cells were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences and cultured in RPMI‐1640 medium (Gibco) supplemented with 10% FBS (ScienCell) and 1% penicillin/streptomycin solution.

Cell Culture:

Article Title: Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition
Article Snippet: .. Cell Culture Human umbilical vein endothelial cell (HUVEC) line 12 was purchased from YRGene (NC006) and cultured in Gibco RPMI1640 medium supplemented with 10% fetal bovine serum (FBS), endothelial cell growth supplement (ECGS, ScienCell, 1052, 10 μ l/ml), and heparin (0.1 mg/ml) in a humidified 5% CO2 incubator at 37°C. .. After 12 hours of starvation for synchronization, HUVECs were preincubated with different concentrations of puerarin (10 μ M, 25 μ M, and 50 μ M) or pioglitazone (20 μ M) in the presence or absence of GW9662 (10 μ M) for 30 minutes and then incubated with recombinant human TGF-β 1 (10 ng/ml) or phosphate buffer saline (PBS) for 48 hours.

Article Title: Gel spinning of silk tubes for tissue engineering
Article Snippet: .. Prior to seeding, they were cultured according to previously reported protocols where GFP-HUVECs were grown in optimized growth media EGM-2 (Lonza, Walkersville, MD) supplemented with 100 U/mL penicillin, 1000 U/mL streptomycin, and 0.2% fungizone antimycotic (GIBCO, Carlsbad, CA), and HCASMCs were cultured in smooth muscle cell medium (SMCM) with 2% fetal bovine serum (FBS), 1% smooth muscle cell growth supplement, and 1% penicillin/streptomycin solution (ScienCell Research Laboratories, Carlsbad, CA). .. Prior to cell seeding, HCASMCs were stained using a red CellTracker dye at a concentration of 10 μM according to company protocols (Invitrogen, Carlsbad, CA).

Article Title: Enhanced store-operated Ca2+ influx and ORAI1 expression in ventricular fibroblasts from human failing heart
Article Snippet: .. The ventricular tissue block was carefully cleaned to remove all non-myocardial portions in a 60 mm culture dish in the tissue culture hood and cut into 1 mm blocks, transferred to a 25 cm2 TPP tissue culture flask (MidSci, St Louis, MO), washed thrice with DPBS, twice with FM-b (ScienCell) containing penicillin/streptomycin, spread evenly into 20-30 blocks per flask and cultured in 5 ml FM-2 media (ScienCell) containing 5% fetal bovine serum (FBS) and penicillin/streptomycin. ..

Article Title: KRIT1 Protein Depletion Modifies Endothelial Cell Behavior via Increased Vascular Endothelial Growth Factor (VEGF) Signaling *
Article Snippet: .. HPAEC (Invitrogen) were cultured in 1:1 Dulbecco's modified Eagle's medium (DMEM):F-12 supplemented with 5% fetal bovine serum (FBS), 1% endothelial cell growth supplement (ECGS, ScienCell, Carlsbad, CA), 1% antimycotic/antibiotic solution (Invitrogen), and 50 μ m heparin (Calbiochem) at 37 °C with 5% CO2 . ..

Article Title: NFAT5 promotes arteriogenesis via MCP‐1‐dependent monocyte recruitment, et al. NFAT5 promotes arteriogenesis via MCP‐1‐dependent monocyte recruitment
Article Snippet: .. 2.5 Cell culture and transfection Human umbilical vein endothelial cells were purchased from ScienCell Research Laboratories, Inc and maintained in endothelial cell medium (ScienCell) supplemented with 5% foetal bovine serum (FBS), endothelial cell growth supplement and 1% penicillin/streptomycin (ScienCell). .. THP‐1 cells were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences and cultured in RPMI‐1640 medium (Gibco) supplemented with 10% FBS (ScienCell) and 1% penicillin/streptomycin solution.

Article Title: LCAT, ApoD, and ApoA1 Expression and Review of Cholesterol Deposition in the Cornea
Article Snippet: .. Western Blot Assay Human keratocytes (ScienCell #6520, Carlsbad, CA, USA) and human primary dermal fibroblasts (ATCC #PCS-201-012, Manassas, VA, USA) were cultured in fibroblast medium (ScienCell #2301) containing 2% fetal bovine serum (FBS), fibroblast growth supplement in an amount specified by the manufacturer (ScienCell #2352,), 50 units/mL of penicillin, and 50 μg/mL of streptomycin (ScienCell #0503). .. HepG2 cells (ATCC #HB-8065) were cultured in RPMI 1640 medium containing 10% FBS.

Blocking Assay:

Article Title: Enhanced store-operated Ca2+ influx and ORAI1 expression in ventricular fibroblasts from human failing heart
Article Snippet: .. The ventricular tissue block was carefully cleaned to remove all non-myocardial portions in a 60 mm culture dish in the tissue culture hood and cut into 1 mm blocks, transferred to a 25 cm2 TPP tissue culture flask (MidSci, St Louis, MO), washed thrice with DPBS, twice with FM-b (ScienCell) containing penicillin/streptomycin, spread evenly into 20-30 blocks per flask and cultured in 5 ml FM-2 media (ScienCell) containing 5% fetal bovine serum (FBS) and penicillin/streptomycin. ..

Modification:

Article Title: KRIT1 Protein Depletion Modifies Endothelial Cell Behavior via Increased Vascular Endothelial Growth Factor (VEGF) Signaling *
Article Snippet: .. HPAEC (Invitrogen) were cultured in 1:1 Dulbecco's modified Eagle's medium (DMEM):F-12 supplemented with 5% fetal bovine serum (FBS), 1% endothelial cell growth supplement (ECGS, ScienCell, Carlsbad, CA), 1% antimycotic/antibiotic solution (Invitrogen), and 50 μ m heparin (Calbiochem) at 37 °C with 5% CO2 . ..

Western Blot:

Article Title: LCAT, ApoD, and ApoA1 Expression and Review of Cholesterol Deposition in the Cornea
Article Snippet: .. Western Blot Assay Human keratocytes (ScienCell #6520, Carlsbad, CA, USA) and human primary dermal fibroblasts (ATCC #PCS-201-012, Manassas, VA, USA) were cultured in fibroblast medium (ScienCell #2301) containing 2% fetal bovine serum (FBS), fibroblast growth supplement in an amount specified by the manufacturer (ScienCell #2352,), 50 units/mL of penicillin, and 50 μg/mL of streptomycin (ScienCell #0503). .. HepG2 cells (ATCC #HB-8065) were cultured in RPMI 1640 medium containing 10% FBS.

Hood:

Article Title: Enhanced store-operated Ca2+ influx and ORAI1 expression in ventricular fibroblasts from human failing heart
Article Snippet: .. The ventricular tissue block was carefully cleaned to remove all non-myocardial portions in a 60 mm culture dish in the tissue culture hood and cut into 1 mm blocks, transferred to a 25 cm2 TPP tissue culture flask (MidSci, St Louis, MO), washed thrice with DPBS, twice with FM-b (ScienCell) containing penicillin/streptomycin, spread evenly into 20-30 blocks per flask and cultured in 5 ml FM-2 media (ScienCell) containing 5% fetal bovine serum (FBS) and penicillin/streptomycin. ..

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  • 92
    ScienCell pericyte medium
    Characterization of each cell type used for establishing BBB organoids. a) Fluorescence images showing the expression of CD31 (Abcam, dilution: 1:100) and VE-Cadherin (Cell Signaling Technology, dilution: 1:100) (endothelial cell markers) in primary human brain microvascular endothelial cells (HBMEC) (ScienCell). b) (Top) Bright field image showing the morphology of immortalized human cerebral microvascular endothelial cells (hCMEC/D3) (Cedarlane), and (middle) fluorescence image showing the expression of CD31. c) (Top) Bright field image of primary human brain vascular pericytes (ScienCell) and (middle) fluorescence image showing the expression of the <t>pericyte</t> marker NG2 (Millipore, dilution: 1:100). d) (Top) Bright field image of primary astrocytes (Lonza) and (middle) fluorescence image showing the expression of astrocyte marker GFAP (Sigma-Aldrich, dilution: 1:100). (b-d, bottom panels) Cells incubated with secondary IgG only (no primary IgG) were used as negative controls. Nuclei of cells were stained with the Hoechst dye (blue). Secondary antibody anti-rabbit Alexa Fluor 546 (red) or anti-mouse Alexa Fluor 488 (green) was used. Scale bar: 100 μm (Bright field: 20x objective; confocal: 40x objective).
    Pericyte Medium, supplied by ScienCell, used in various techniques. Bioz Stars score: 92/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pericyte medium - by Bioz Stars, 2020-07
    92/100 stars
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    93
    ScienCell fetal bovine serum fbs
    Puerarin inhibited TGF-β1-induced HUVECs migration rate . HUVECs were preincubated with different concentrations of puerarin (10, 25, and 50 μ M) for 30 min and then treated with TGF- β 1 (10 ng/ml) for 48 h until a monolayer was formed. Scratches were made straightly across the dishes. After rinsing twice, the medium was replaced by <t>RPMI1640</t> with no <t>FBS.</t> Photographs were taken at indicated times (scale bars: 50 μ m).
    Fetal Bovine Serum Fbs, supplied by ScienCell, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal bovine serum fbs/product/ScienCell
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum fbs - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    ScienCell human brain pericytes
    Imatinib treatment of human DLBCL tumors was associated with pericytic dropout and disruption of microvasculature. (A1-A4) Analysis of α-SMA + <t>pericytes</t> and CD31 + vessels in (A1,A3) Karpas422 and (A2,A4) OCI-Ly7 xenografts. (B1-B4) Analysis of
    Human Brain Pericytes, supplied by ScienCell, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 5 article reviews
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    human brain pericytes - by Bioz Stars, 2020-07
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    Image Search Results


    Characterization of each cell type used for establishing BBB organoids. a) Fluorescence images showing the expression of CD31 (Abcam, dilution: 1:100) and VE-Cadherin (Cell Signaling Technology, dilution: 1:100) (endothelial cell markers) in primary human brain microvascular endothelial cells (HBMEC) (ScienCell). b) (Top) Bright field image showing the morphology of immortalized human cerebral microvascular endothelial cells (hCMEC/D3) (Cedarlane), and (middle) fluorescence image showing the expression of CD31. c) (Top) Bright field image of primary human brain vascular pericytes (ScienCell) and (middle) fluorescence image showing the expression of the pericyte marker NG2 (Millipore, dilution: 1:100). d) (Top) Bright field image of primary astrocytes (Lonza) and (middle) fluorescence image showing the expression of astrocyte marker GFAP (Sigma-Aldrich, dilution: 1:100). (b-d, bottom panels) Cells incubated with secondary IgG only (no primary IgG) were used as negative controls. Nuclei of cells were stained with the Hoechst dye (blue). Secondary antibody anti-rabbit Alexa Fluor 546 (red) or anti-mouse Alexa Fluor 488 (green) was used. Scale bar: 100 μm (Bright field: 20x objective; confocal: 40x objective).

    Journal: Nature protocols

    Article Title: Blood-brain-barrier organoids for investigating the permeability of CNS therapeutics

    doi: 10.1038/s41596-018-0066-x

    Figure Lengend Snippet: Characterization of each cell type used for establishing BBB organoids. a) Fluorescence images showing the expression of CD31 (Abcam, dilution: 1:100) and VE-Cadherin (Cell Signaling Technology, dilution: 1:100) (endothelial cell markers) in primary human brain microvascular endothelial cells (HBMEC) (ScienCell). b) (Top) Bright field image showing the morphology of immortalized human cerebral microvascular endothelial cells (hCMEC/D3) (Cedarlane), and (middle) fluorescence image showing the expression of CD31. c) (Top) Bright field image of primary human brain vascular pericytes (ScienCell) and (middle) fluorescence image showing the expression of the pericyte marker NG2 (Millipore, dilution: 1:100). d) (Top) Bright field image of primary astrocytes (Lonza) and (middle) fluorescence image showing the expression of astrocyte marker GFAP (Sigma-Aldrich, dilution: 1:100). (b-d, bottom panels) Cells incubated with secondary IgG only (no primary IgG) were used as negative controls. Nuclei of cells were stained with the Hoechst dye (blue). Secondary antibody anti-rabbit Alexa Fluor 546 (red) or anti-mouse Alexa Fluor 488 (green) was used. Scale bar: 100 μm (Bright field: 20x objective; confocal: 40x objective).

    Article Snippet: Pericyte medium (includes 500 mL of pericyte basal medium, 10 mL of FBS, 5 mL of pericyte growth supplements (PGS) and 5 mL of penicillin/streptomycin solution) (ScienCell, cat. no. 1201).

    Techniques: Fluorescence, Expressing, Marker, Incubation, Staining

    Culture media modifies phagocytic capacity of human brain pericytes. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. Cells were incubated with Fluoresbrite ® YG carboxylate microspheres of 1–2 µm diameter for 2 h and the phagocytic capacity of pericytes determined by flow cytometry. One representative plot for 1 µm beads ( a ) and 2 µm beads ( b ) is shown. The mean fluorescent intensity (MFI) from three independent experiments was determined ( c ). Pericytes were treated as above, except beads were incubated with cells for 24 h, fixed and nuclei counterstained with DRAQ5. Fluorescent microscopy images of one representative case from three independent experiments are shown ( d ). Data are displayed as mean ± SEM of three independent experiments. NS = p > 0.05; **p

    Journal: BMC Neuroscience

    Article Title: Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

    doi: 10.1186/s12868-018-0405-4

    Figure Lengend Snippet: Culture media modifies phagocytic capacity of human brain pericytes. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. Cells were incubated with Fluoresbrite ® YG carboxylate microspheres of 1–2 µm diameter for 2 h and the phagocytic capacity of pericytes determined by flow cytometry. One representative plot for 1 µm beads ( a ) and 2 µm beads ( b ) is shown. The mean fluorescent intensity (MFI) from three independent experiments was determined ( c ). Pericytes were treated as above, except beads were incubated with cells for 24 h, fixed and nuclei counterstained with DRAQ5. Fluorescent microscopy images of one representative case from three independent experiments are shown ( d ). Data are displayed as mean ± SEM of three independent experiments. NS = p > 0.05; **p

    Article Snippet: DMEM containing 10–20% FBS and defined Pericyte Medium (ScienCell) have been used in the majority of recent in vitro pericyte-related studies.

    Techniques: Incubation, Flow Cytometry, Cytometry, Microscopy

    Culture media modifies pericyte migration following a scratch wound injury. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7–14 days. The resulting confluent pericyte monolayer was scratched down the centre of the well using a sterile 200 µL pipette tip and wells were washed twice with PBS to remove detached cells. An equal number of wells were left unscratched. Pericytes were incubated for a further 48 h to allow migration into the scratch wound to occur. Cells were fixed and stained with Coomassie blue and imaged by bright field microscopy. Consistency of scratches between wells can be observed ( a ). A magnified image of a representative well shows the difference in pericyte migration ( b ). The gap area, defined as the percentage of each site devoid of Coomassie staining, was determined by automated image analysis ( c ). Data are displayed as mean ± SEM of three independent experiments. *p

    Journal: BMC Neuroscience

    Article Title: Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

    doi: 10.1186/s12868-018-0405-4

    Figure Lengend Snippet: Culture media modifies pericyte migration following a scratch wound injury. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7–14 days. The resulting confluent pericyte monolayer was scratched down the centre of the well using a sterile 200 µL pipette tip and wells were washed twice with PBS to remove detached cells. An equal number of wells were left unscratched. Pericytes were incubated for a further 48 h to allow migration into the scratch wound to occur. Cells were fixed and stained with Coomassie blue and imaged by bright field microscopy. Consistency of scratches between wells can be observed ( a ). A magnified image of a representative well shows the difference in pericyte migration ( b ). The gap area, defined as the percentage of each site devoid of Coomassie staining, was determined by automated image analysis ( c ). Data are displayed as mean ± SEM of three independent experiments. *p

    Article Snippet: DMEM containing 10–20% FBS and defined Pericyte Medium (ScienCell) have been used in the majority of recent in vitro pericyte-related studies.

    Techniques: Migration, Transferring, Incubation, Staining, Microscopy

    Culture medium does not alter pericyte responsiveness to growth factors TGFβ 1 and PDGF-BB. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 2 h pericytes were incubated with 10 ng/mL TGFβ 1 or vehicle (1 µM citric acid, pH 3 with 0.0001% BSA). Cells were fixed and immunostained for SMAD2/3 ( a ) and the nuclear intensity was determined by automated imagining analysis ( b ). Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 30 min pericytes were treated with 10–100 ng/mL PDGF-BB or vehicle (4 µM hydrochloric acid with 0.0001% BSA). Cells were brought into suspension with Accutase and incubated with a PDGFRβ antibody or an isotype control. Cell surface PDGFRβ was determined by flow cytometry ( c ). Data are displayed as mean ± SEM of three independent experiments. ***p

    Journal: BMC Neuroscience

    Article Title: Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

    doi: 10.1186/s12868-018-0405-4

    Figure Lengend Snippet: Culture medium does not alter pericyte responsiveness to growth factors TGFβ 1 and PDGF-BB. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 2 h pericytes were incubated with 10 ng/mL TGFβ 1 or vehicle (1 µM citric acid, pH 3 with 0.0001% BSA). Cells were fixed and immunostained for SMAD2/3 ( a ) and the nuclear intensity was determined by automated imagining analysis ( b ). Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 30 min pericytes were treated with 10–100 ng/mL PDGF-BB or vehicle (4 µM hydrochloric acid with 0.0001% BSA). Cells were brought into suspension with Accutase and incubated with a PDGFRβ antibody or an isotype control. Cell surface PDGFRβ was determined by flow cytometry ( c ). Data are displayed as mean ± SEM of three independent experiments. ***p

    Article Snippet: DMEM containing 10–20% FBS and defined Pericyte Medium (ScienCell) have been used in the majority of recent in vitro pericyte-related studies.

    Techniques: Incubation, Flow Cytometry, Cytometry

    Culture media alters pericyte phenotype and proliferation. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium. Brightfield imaging of pericyte morphology was performed 3 and 7 days post plating ( a ). Pericytes were then fixed and the nuclei stained with Hoechst. The average nuclei area ( b ) and the total cell count ( c ) were determined by automated image analysis. Five days after plating, 10 µM EdU was added to select wells for an additional 48 h. Cells were fixed and nuclei counterstained with Hoechst ( d ). The percentage of EdU positive cells was determined by automated image analysis ( e ). Data are displayed as mean ± SEM of three independent experiments. ***p

    Journal: BMC Neuroscience

    Article Title: Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

    doi: 10.1186/s12868-018-0405-4

    Figure Lengend Snippet: Culture media alters pericyte phenotype and proliferation. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium. Brightfield imaging of pericyte morphology was performed 3 and 7 days post plating ( a ). Pericytes were then fixed and the nuclei stained with Hoechst. The average nuclei area ( b ) and the total cell count ( c ) were determined by automated image analysis. Five days after plating, 10 µM EdU was added to select wells for an additional 48 h. Cells were fixed and nuclei counterstained with Hoechst ( d ). The percentage of EdU positive cells was determined by automated image analysis ( e ). Data are displayed as mean ± SEM of three independent experiments. ***p

    Article Snippet: DMEM containing 10–20% FBS and defined Pericyte Medium (ScienCell) have been used in the majority of recent in vitro pericyte-related studies.

    Techniques: Imaging, Staining, Cell Counting

    Culture media modifies expression of typical pericyte markers. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. At completion, pericytes were fixed and immunostained for the pericyte-associated markers CD146, αSMA, P4H, desmin, fibronectin, NG2, PDGFRβ and COL-IV. Nuclei were counterstained with Hoechst ( a ). The integrated intensity/cell of pericyte marker expression was determined by automated image analysis ( b ). RNA was extracted from samples treated as above and qRT-PCR performed to determine pericyte marker gene expression ( c ). Data are displayed as mean ± SEM of three independent experiments. **p

    Journal: BMC Neuroscience

    Article Title: Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

    doi: 10.1186/s12868-018-0405-4

    Figure Lengend Snippet: Culture media modifies expression of typical pericyte markers. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. At completion, pericytes were fixed and immunostained for the pericyte-associated markers CD146, αSMA, P4H, desmin, fibronectin, NG2, PDGFRβ and COL-IV. Nuclei were counterstained with Hoechst ( a ). The integrated intensity/cell of pericyte marker expression was determined by automated image analysis ( b ). RNA was extracted from samples treated as above and qRT-PCR performed to determine pericyte marker gene expression ( c ). Data are displayed as mean ± SEM of three independent experiments. **p

    Article Snippet: DMEM containing 10–20% FBS and defined Pericyte Medium (ScienCell) have been used in the majority of recent in vitro pericyte-related studies.

    Techniques: Expressing, Marker, Quantitative RT-PCR

    Culture media modifies IL-1β induced inflammatory mediator expression in human brain pericytes. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 24 h 10 ng/mL IL-1β or vehicle (0.0001% BSA in PBS) was added. Pericytes were fixed and immunostained for the inflammatory mediators ICAM-1 or MCP-1 ( a ). Nuclei were counterstained with Hoechst. The integrated intensity of ICAM-1/cell was determined by automated image analysis ( b ), the secretion of soluble ICAM-1 (sICAM-1) was determined by a cytometric bead array ( c ) and the gene expression of ICAM1 was determined by qRT-PCR ( d ). Similarly, the integrated intensity of MCP-1/cell was determined by automated image analysis ( e ), the secretion of MCP-1 was determined by a cytometric bead array ( f ) and the gene expression of MCP1 determined by qRT-PCR ( g ). Data are displayed as mean ± SEM from three independent experiments except CBA data which depicts mean ± SEM of triplicate wells from one representative case of three independent experiments. NS = p > 0.05; **p

    Journal: BMC Neuroscience

    Article Title: Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

    doi: 10.1186/s12868-018-0405-4

    Figure Lengend Snippet: Culture media modifies IL-1β induced inflammatory mediator expression in human brain pericytes. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 24 h 10 ng/mL IL-1β or vehicle (0.0001% BSA in PBS) was added. Pericytes were fixed and immunostained for the inflammatory mediators ICAM-1 or MCP-1 ( a ). Nuclei were counterstained with Hoechst. The integrated intensity of ICAM-1/cell was determined by automated image analysis ( b ), the secretion of soluble ICAM-1 (sICAM-1) was determined by a cytometric bead array ( c ) and the gene expression of ICAM1 was determined by qRT-PCR ( d ). Similarly, the integrated intensity of MCP-1/cell was determined by automated image analysis ( e ), the secretion of MCP-1 was determined by a cytometric bead array ( f ) and the gene expression of MCP1 determined by qRT-PCR ( g ). Data are displayed as mean ± SEM from three independent experiments except CBA data which depicts mean ± SEM of triplicate wells from one representative case of three independent experiments. NS = p > 0.05; **p

    Article Snippet: DMEM containing 10–20% FBS and defined Pericyte Medium (ScienCell) have been used in the majority of recent in vitro pericyte-related studies.

    Techniques: Expressing, Quantitative RT-PCR, Crocin Bleaching Assay

    Culture media modifies IL-1β-induced transcription factor expression in human brain pericytes. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 1 h ( a ) or 24 h ( b ) 10 ng/mL IL-1β or vehicle was added. Pericytes were fixed and immunostained for the transcription factors NF-kB p65 ( a ) or C/EBPδ ( b ). Nuclei were counterstained with Hoechst. The percentage of cells displaying nuclear NF-kB p65 ( c ) or scored positive for C/EBPδ ( d ) was determined by automated image analysis. RNA was extracted from pericytes treated with 10 ng/mL IL-1β or vehicle (0.0001% BSA in PBS) for the final 24 h of a 7 day culture and the gene expression of CEBPD determined by qRT-PCR ( e ). Data are displayed as mean ± SEM of three independent experiments. NS = p > 0.05; **p

    Journal: BMC Neuroscience

    Article Title: Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

    doi: 10.1186/s12868-018-0405-4

    Figure Lengend Snippet: Culture media modifies IL-1β-induced transcription factor expression in human brain pericytes. Human brain pericytes were plated at a low density and allowed to proliferate in either DMEM/F12 with 10% FBS or Pericyte Medium for 7 days. For the final 1 h ( a ) or 24 h ( b ) 10 ng/mL IL-1β or vehicle was added. Pericytes were fixed and immunostained for the transcription factors NF-kB p65 ( a ) or C/EBPδ ( b ). Nuclei were counterstained with Hoechst. The percentage of cells displaying nuclear NF-kB p65 ( c ) or scored positive for C/EBPδ ( d ) was determined by automated image analysis. RNA was extracted from pericytes treated with 10 ng/mL IL-1β or vehicle (0.0001% BSA in PBS) for the final 24 h of a 7 day culture and the gene expression of CEBPD determined by qRT-PCR ( e ). Data are displayed as mean ± SEM of three independent experiments. NS = p > 0.05; **p

    Article Snippet: DMEM containing 10–20% FBS and defined Pericyte Medium (ScienCell) have been used in the majority of recent in vitro pericyte-related studies.

    Techniques: Expressing, Quantitative RT-PCR

    Puerarin inhibited TGF-β1-induced HUVECs migration rate . HUVECs were preincubated with different concentrations of puerarin (10, 25, and 50 μ M) for 30 min and then treated with TGF- β 1 (10 ng/ml) for 48 h until a monolayer was formed. Scratches were made straightly across the dishes. After rinsing twice, the medium was replaced by RPMI1640 with no FBS. Photographs were taken at indicated times (scale bars: 50 μ m).

    Journal: PPAR Research

    Article Title: Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition

    doi: 10.1155/2017/2647129

    Figure Lengend Snippet: Puerarin inhibited TGF-β1-induced HUVECs migration rate . HUVECs were preincubated with different concentrations of puerarin (10, 25, and 50 μ M) for 30 min and then treated with TGF- β 1 (10 ng/ml) for 48 h until a monolayer was formed. Scratches were made straightly across the dishes. After rinsing twice, the medium was replaced by RPMI1640 with no FBS. Photographs were taken at indicated times (scale bars: 50 μ m).

    Article Snippet: Cell Culture Human umbilical vein endothelial cell (HUVEC) line 12 was purchased from YRGene (NC006) and cultured in Gibco RPMI1640 medium supplemented with 10% fetal bovine serum (FBS), endothelial cell growth supplement (ECGS, ScienCell, 1052, 10 μ l/ml), and heparin (0.1 mg/ml) in a humidified 5% CO2 incubator at 37°C.

    Techniques: Migration

    Imatinib treatment of human DLBCL tumors was associated with pericytic dropout and disruption of microvasculature. (A1-A4) Analysis of α-SMA + pericytes and CD31 + vessels in (A1,A3) Karpas422 and (A2,A4) OCI-Ly7 xenografts. (B1-B4) Analysis of

    Journal: Blood

    Article Title: Imatinib disrupts lymphoma angiogenesis by targeting vascular pericytes

    doi: 10.1182/blood-2013-03-490763

    Figure Lengend Snippet: Imatinib treatment of human DLBCL tumors was associated with pericytic dropout and disruption of microvasculature. (A1-A4) Analysis of α-SMA + pericytes and CD31 + vessels in (A1,A3) Karpas422 and (A2,A4) OCI-Ly7 xenografts. (B1-B4) Analysis of

    Article Snippet: The primary murine VSMCs (see supplemental Materials and Methods on the Blood website) were grown in DMEM containing 10% FBS with P/S, whereas the primary human brain pericytes were purchased from ScienCell and grown in its proprietary pericyte culture medium.

    Techniques:

    Imatinib treatment of murine EL4 tumors impaired growth, decreased pericyte coverage, and reduced vascularization. (A) Tumor weight (g) at the time of tissue harvest, comparing imatinib (red) vs PBS (black). (B) Analysis of pericytes with (1-2) NG2, (3-4)

    Journal: Blood

    Article Title: Imatinib disrupts lymphoma angiogenesis by targeting vascular pericytes

    doi: 10.1182/blood-2013-03-490763

    Figure Lengend Snippet: Imatinib treatment of murine EL4 tumors impaired growth, decreased pericyte coverage, and reduced vascularization. (A) Tumor weight (g) at the time of tissue harvest, comparing imatinib (red) vs PBS (black). (B) Analysis of pericytes with (1-2) NG2, (3-4)

    Article Snippet: The primary murine VSMCs (see supplemental Materials and Methods on the Blood website) were grown in DMEM containing 10% FBS with P/S, whereas the primary human brain pericytes were purchased from ScienCell and grown in its proprietary pericyte culture medium.

    Techniques: