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PAA Laboratories fetal bovine serum fbs
Dose-dependent effects of celastrol on cellular viability in MCF-7, HepG2, and THP-1. Cells were seeded overnight in 96-well plates at a density of 2 × 10 5 /ml in phenol red-free <t>DMEM</t> supplemented with 5% charcoal absorbed <t>FBS,</t>
Fetal Bovine Serum Fbs, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 233 article reviews
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fetal bovine serum fbs - by Bioz Stars, 2020-07
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1) Product Images from "Celastrol regulates multiple nuclear transcription factors belonging to HSP90's clients in a dose- and cell type-dependent way"

Article Title: Celastrol regulates multiple nuclear transcription factors belonging to HSP90's clients in a dose- and cell type-dependent way

Journal: Cell Stress & Chaperones

doi: 10.1007/s12192-010-0202-1

Dose-dependent effects of celastrol on cellular viability in MCF-7, HepG2, and THP-1. Cells were seeded overnight in 96-well plates at a density of 2 × 10 5 /ml in phenol red-free DMEM supplemented with 5% charcoal absorbed FBS,
Figure Legend Snippet: Dose-dependent effects of celastrol on cellular viability in MCF-7, HepG2, and THP-1. Cells were seeded overnight in 96-well plates at a density of 2 × 10 5 /ml in phenol red-free DMEM supplemented with 5% charcoal absorbed FBS,

Techniques Used:

2) Product Images from "Corexit 9500 Inactivates Two Enveloped Viruses of Aquatic Animals but Enhances the Infectivity of a Nonenveloped Fish Virus"

Article Title: Corexit 9500 Inactivates Two Enveloped Viruses of Aquatic Animals but Enhances the Infectivity of a Nonenveloped Fish Virus

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.03569-13

Cytotoxic effect of Corexit 9500 on EPC and CHSE-214. EPC (a) or CHSE-214 (b) cells were exposed to 10-fold serial TCID 50 dilutions, up to 10 −6 , of Corexit 9500 (10%, initial concentration) in L15 with 2% FBS before cell viability was determined
Figure Legend Snippet: Cytotoxic effect of Corexit 9500 on EPC and CHSE-214. EPC (a) or CHSE-214 (b) cells were exposed to 10-fold serial TCID 50 dilutions, up to 10 −6 , of Corexit 9500 (10%, initial concentration) in L15 with 2% FBS before cell viability was determined

Techniques Used: Concentration Assay

3) Product Images from "Efficient myoblast expansion for regenerative medicine use"

Article Title: Efficient myoblast expansion for regenerative medicine use

Journal: International Journal of Molecular Medicine

doi: 10.3892/ijmm.2014.1763

Fusion potential of cultured myoblasts in different media. After passage 3, cells cultured in 2 different media were seeded in duplicate into 96-well plates, and when 80% confluence was obtained, the culture medium was switched to fusion medium [Dulbecco’s modified Eagle’s medium (DMEM), 2% fetal bovine serum (FBS), 25 μ mol/l insulin] and changed every 3 days. After 11 days, cells were fixed and stained with Wright’s eosin methylene blue solution. The number of nuclei in the 6 largest myotubes was counted in each well. Additionally, cells cultured in DFEFH medium were subjected to higher passages and their fusogenic potential was assessed in a similar manner. (A) Comparison of the fusogenic potential of myoblasts cultured in skeletal muscle cell growth medium-2 (SKGM-2) and DFEFH medium at passage 3. Representative results of 2 independent experiments are shown. (B) The average number of nuclei in the largest myotubes created from myoblasts expanded in SKGM-2 and DFEFH medium at passage 3, n=2 (2 different donors); * p
Figure Legend Snippet: Fusion potential of cultured myoblasts in different media. After passage 3, cells cultured in 2 different media were seeded in duplicate into 96-well plates, and when 80% confluence was obtained, the culture medium was switched to fusion medium [Dulbecco’s modified Eagle’s medium (DMEM), 2% fetal bovine serum (FBS), 25 μ mol/l insulin] and changed every 3 days. After 11 days, cells were fixed and stained with Wright’s eosin methylene blue solution. The number of nuclei in the 6 largest myotubes was counted in each well. Additionally, cells cultured in DFEFH medium were subjected to higher passages and their fusogenic potential was assessed in a similar manner. (A) Comparison of the fusogenic potential of myoblasts cultured in skeletal muscle cell growth medium-2 (SKGM-2) and DFEFH medium at passage 3. Representative results of 2 independent experiments are shown. (B) The average number of nuclei in the largest myotubes created from myoblasts expanded in SKGM-2 and DFEFH medium at passage 3, n=2 (2 different donors); * p

Techniques Used: Cell Culture, Modification, Staining

4) Product Images from "miR-506 inhibits cell proliferation and invasion by targeting TET family in colorectal cancer"

Article Title: miR-506 inhibits cell proliferation and invasion by targeting TET family in colorectal cancer

Journal: Iranian Journal of Basic Medical Sciences

doi:

Knockdown of TET genes inhibits CRC cell growth and invasion in vitro . (A) SW620 cells were transfected with sh-ctr, sh-TET1, shTET2 or shTET3. Then they were seeded in 12-well plates at the desired cell concentration and maintained in medium containing 10% fetal bovine serum. The cells were counted at the indicated time points, and their growth rates were recorded. Experiments were performed in triplicate. (B) Invasion analyses were performed in SW620 cells that were infected with TET1/2/3 shRNA or sh-ctr RNA. Expression images displaying TET1/2/3 are shown. Scale bars: 50 µm
Figure Legend Snippet: Knockdown of TET genes inhibits CRC cell growth and invasion in vitro . (A) SW620 cells were transfected with sh-ctr, sh-TET1, shTET2 or shTET3. Then they were seeded in 12-well plates at the desired cell concentration and maintained in medium containing 10% fetal bovine serum. The cells were counted at the indicated time points, and their growth rates were recorded. Experiments were performed in triplicate. (B) Invasion analyses were performed in SW620 cells that were infected with TET1/2/3 shRNA or sh-ctr RNA. Expression images displaying TET1/2/3 are shown. Scale bars: 50 µm

Techniques Used: In Vitro, Transfection, Concentration Assay, Infection, shRNA, RNA Expression

Related Articles

Modification:

Article Title: Celastrol regulates multiple nuclear transcription factors belonging to HSP90's clients in a dose- and cell type-dependent way
Article Snippet: .. RPMI 1640 medium, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and streptomycin/penicillin for cell culture use were obtained from PAA Laboratories (Linz, Austria). .. DMEM with no phenol red was obtained from Gibco (Invitrogen Co, Carlsbad, CA).

Article Title: miR-506 inhibits cell proliferation and invasion by targeting TET family in colorectal cancer
Article Snippet: .. SW480, HCT15, HCT116, KM12, NCM460, DLD1 and Ls174t cells were cultured in RPMI-1640 medium (Gibco, USA) containing 10% fetal bovine serum (FBS) (PAA Laboratories, Austria), and SW620 and HT29 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum. ..

other:

Article Title: Corexit 9500 Inactivates Two Enveloped Viruses of Aquatic Animals but Enhances the Infectivity of a Nonenveloped Fish Virus
Article Snippet: Both cell lines were grown in 75-cm2 flasks (BD Biosciences, Fisher Scientific) using Leibovitz's L15 medium (HyClone; Fisher Scientific, Mississauga, ON, Canada) supplemented with 10% fetal bovine serum (FBS) (PAA Laboratories, VWR International, Mississauga, ON, Canada) and 1% penicillin-streptomycin (PS) (HyClone; Fisher Scientific, Mississauga, ON, Canada).

Article Title: Cholesterol Oxidase Binds TLR2 and Modulates Functional Responses of Human Macrophages
Article Snippet: Human type AB serum (off-the-clot) and fetal bovine serum (FBS) were purchased from PAA Laboratories (Pasching, Austria).

Article Title: MicroRNA Controlled Adenovirus Mediates Anti-Cancer Efficacy without Affecting Endogenous MicroRNA Activity
Article Snippet: Human hepatocellular carcinoma HepG2 and Hep3b cell lines and A549 lung carcinoma cells were obtained from the European Collection of Cell Cultures (Porton Down, UK), and maintained in DMEM with 10% foetal bovine serum (FBS) (PAA Laboratories, Yeovil, UK) including penicillin (25 U/ml) and streptomycin (10 mg/ml).

Cell Culture:

Article Title: Celastrol regulates multiple nuclear transcription factors belonging to HSP90's clients in a dose- and cell type-dependent way
Article Snippet: .. RPMI 1640 medium, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and streptomycin/penicillin for cell culture use were obtained from PAA Laboratories (Linz, Austria). .. DMEM with no phenol red was obtained from Gibco (Invitrogen Co, Carlsbad, CA).

Article Title: miR-506 inhibits cell proliferation and invasion by targeting TET family in colorectal cancer
Article Snippet: .. SW480, HCT15, HCT116, KM12, NCM460, DLD1 and Ls174t cells were cultured in RPMI-1640 medium (Gibco, USA) containing 10% fetal bovine serum (FBS) (PAA Laboratories, Austria), and SW620 and HT29 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco) supplemented with 10% fetal bovine serum. ..

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  • 92
    PAA Laboratories charcoal stripped fbs
    AB215 induced IDs cooperatively inhibit E2 activating P-ERK1/2. MCF7 cells were reverse transfected with siRNA in serum free/phenol red free <t>RPMI1640</t> and plated in phenol red free RPMI1640 supplemented with 10% heat inactivated charcoal-stripped <t>FBS.</t> Control was transfected with non-sense siRNA. After 24 hours of transfection, cells were treated with or without 10nM E2 along with 500 ng/ml BMP2 or AB215. After 48 hours of exposure, RNA was extracted for qRT-PCR analysis or harvested for western blot analysis. A) KD efficiency of each IDs using siRNA. mRNA level of each IDs were measured using qRT-PCR to confirm the effectiveness of mRNA knocked down. Data are shown as means + SD in quadruplicates, n = 3. B) KD samples are analyzed for western blot to detect phosphorylation of ERK1/2. The figure shown is done in two separate blots (Control, ID1, ID2-KDs in one blot and ID3, ID4-KDs in another blot). ( * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001 ).
    Charcoal Stripped Fbs, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/charcoal stripped fbs/product/PAA Laboratories
    Average 92 stars, based on 4 article reviews
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    90
    PAA Laboratories dextran charcoal stripped fbs
    The PI3K regulatory subunit genes PIK3R1 and PIK3R3 are direct targets of the AR (A) <t>LNCaP</t> cells were cultured in medium supplemented with 10% dextran charcoal stripped <t>FBS</t> to produce a steroid deplete medium (SD). Following culture for 72 hours, cells were treated with 10 nM synthetic androgen analogue methyltrienolone (R1881) for 24 hours (A+). Relative expression of PIK3R1 , PIK3R3 and PIK3CA was detected by real-time PCR (B) Expression of PIK3R1 , PIK3R3 and PIK3CA mRNA in cells grown in steroid deplete (SD) or androgen (A+) treated conditions over a 24 hours period. The response to androgens was confirmed using PSA (KLK3) expression (not shown). (C) Repression of PIK3R1 and PIK3R3 is also evident in LNCaP cells treated with 0.1 to 100 nM of R1881. (D) The reduction in PIK3R1 and PIK3R3 mRNA expression in response to androgens is still seen in the presence of 1 μg/ml cycloheximide (CHX) as confirmed by real-time PCR.
    Dextran Charcoal Stripped Fbs, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dextran charcoal stripped fbs/product/PAA Laboratories
    Average 90 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
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    94
    PAA Laboratories fbs
    In vitro adherent cells from decidual tissues. (a) Representative photomicrograph of spindle-shaped adherent cells isolated by <t>α</t> MEM and 10% <t>FBS.</t> (b) Cells isolated in vitro by serum-deprived medium (Quantum 333) at early passages. (c) Another population of cells was in vitro isolated contextually with EDT-MSC. These elements share similarities with endothelial cells forming a monolayer of polygonal, sometimes binucleated cells. Original magnification 100x.
    Fbs, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 533 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs/product/PAA Laboratories
    Average 94 stars, based on 533 article reviews
    Price from $9.99 to $1999.99
    fbs - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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    AB215 induced IDs cooperatively inhibit E2 activating P-ERK1/2. MCF7 cells were reverse transfected with siRNA in serum free/phenol red free RPMI1640 and plated in phenol red free RPMI1640 supplemented with 10% heat inactivated charcoal-stripped FBS. Control was transfected with non-sense siRNA. After 24 hours of transfection, cells were treated with or without 10nM E2 along with 500 ng/ml BMP2 or AB215. After 48 hours of exposure, RNA was extracted for qRT-PCR analysis or harvested for western blot analysis. A) KD efficiency of each IDs using siRNA. mRNA level of each IDs were measured using qRT-PCR to confirm the effectiveness of mRNA knocked down. Data are shown as means + SD in quadruplicates, n = 3. B) KD samples are analyzed for western blot to detect phosphorylation of ERK1/2. The figure shown is done in two separate blots (Control, ID1, ID2-KDs in one blot and ID3, ID4-KDs in another blot). ( * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001 ).

    Journal: BMC Cancer

    Article Title: An Activin A/BMP2 chimera, AB215, blocks estrogen signaling via induction of ID proteins in breast cancer cells

    doi: 10.1186/1471-2407-14-549

    Figure Lengend Snippet: AB215 induced IDs cooperatively inhibit E2 activating P-ERK1/2. MCF7 cells were reverse transfected with siRNA in serum free/phenol red free RPMI1640 and plated in phenol red free RPMI1640 supplemented with 10% heat inactivated charcoal-stripped FBS. Control was transfected with non-sense siRNA. After 24 hours of transfection, cells were treated with or without 10nM E2 along with 500 ng/ml BMP2 or AB215. After 48 hours of exposure, RNA was extracted for qRT-PCR analysis or harvested for western blot analysis. A) KD efficiency of each IDs using siRNA. mRNA level of each IDs were measured using qRT-PCR to confirm the effectiveness of mRNA knocked down. Data are shown as means + SD in quadruplicates, n = 3. B) KD samples are analyzed for western blot to detect phosphorylation of ERK1/2. The figure shown is done in two separate blots (Control, ID1, ID2-KDs in one blot and ID3, ID4-KDs in another blot). ( * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001 ).

    Article Snippet: 1×105 cells in RPMI1640 supplemented with10% heat-inactivated and charcoal-stripped FBS were added to the mixture in each well in a 12 well plate.

    Techniques: Transfection, Quantitative RT-PCR, Western Blot

    Anti-proliferative property of AB215 on ERα + breast cancer cells. A, B) ERα + : MCF7, C, D) T47D and E, F) ERα − : SK-BR-3, cells were grown in phenol red free RPMI1640 supplemented with 2% heat inactivated charcoal-stripped FBS treated with or without 10nM E2 along with 500 ng/ml BMP2 or AB215 in quintuplicate. Cell proliferation was analyzed by MTT assay (Abs590-700 nm) on 0, 1, 3 and 5 days after the treatment (n = 3). E2 and AB215 did not affect the proliferation of ERα − cells significantly. The results are presented as means ± SD and their significance has been analyzed by one-way ANOVA. G) MTT assay of MCF7 and T47D cells at increasing concentration of AB215 in the presence of 10nM E2. Cells were plated and treated as explained in Figure 2a-f. Cells were analyzed 4 days after the treatment. Data are shown in means + SD. H) Western blot analysis of E2 induced phosphorylation of ERK1/2. Cells were plated in phenol red free RPMI1640 supplemented with 5% heat inactivated charcoal-stripped FBS treated with or without 10nM E2 along with 500 ng/ml BMP2 or AB215. Cells were harvested and lysed after 48 hours of exposure for western blot analysis. ( * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001 ).

    Journal: BMC Cancer

    Article Title: An Activin A/BMP2 chimera, AB215, blocks estrogen signaling via induction of ID proteins in breast cancer cells

    doi: 10.1186/1471-2407-14-549

    Figure Lengend Snippet: Anti-proliferative property of AB215 on ERα + breast cancer cells. A, B) ERα + : MCF7, C, D) T47D and E, F) ERα − : SK-BR-3, cells were grown in phenol red free RPMI1640 supplemented with 2% heat inactivated charcoal-stripped FBS treated with or without 10nM E2 along with 500 ng/ml BMP2 or AB215 in quintuplicate. Cell proliferation was analyzed by MTT assay (Abs590-700 nm) on 0, 1, 3 and 5 days after the treatment (n = 3). E2 and AB215 did not affect the proliferation of ERα − cells significantly. The results are presented as means ± SD and their significance has been analyzed by one-way ANOVA. G) MTT assay of MCF7 and T47D cells at increasing concentration of AB215 in the presence of 10nM E2. Cells were plated and treated as explained in Figure 2a-f. Cells were analyzed 4 days after the treatment. Data are shown in means + SD. H) Western blot analysis of E2 induced phosphorylation of ERK1/2. Cells were plated in phenol red free RPMI1640 supplemented with 5% heat inactivated charcoal-stripped FBS treated with or without 10nM E2 along with 500 ng/ml BMP2 or AB215. Cells were harvested and lysed after 48 hours of exposure for western blot analysis. ( * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001 ).

    Article Snippet: 1×105 cells in RPMI1640 supplemented with10% heat-inactivated and charcoal-stripped FBS were added to the mixture in each well in a 12 well plate.

    Techniques: MTT Assay, Concentration Assay, Western Blot

    The PI3K regulatory subunit genes PIK3R1 and PIK3R3 are direct targets of the AR (A) LNCaP cells were cultured in medium supplemented with 10% dextran charcoal stripped FBS to produce a steroid deplete medium (SD). Following culture for 72 hours, cells were treated with 10 nM synthetic androgen analogue methyltrienolone (R1881) for 24 hours (A+). Relative expression of PIK3R1 , PIK3R3 and PIK3CA was detected by real-time PCR (B) Expression of PIK3R1 , PIK3R3 and PIK3CA mRNA in cells grown in steroid deplete (SD) or androgen (A+) treated conditions over a 24 hours period. The response to androgens was confirmed using PSA (KLK3) expression (not shown). (C) Repression of PIK3R1 and PIK3R3 is also evident in LNCaP cells treated with 0.1 to 100 nM of R1881. (D) The reduction in PIK3R1 and PIK3R3 mRNA expression in response to androgens is still seen in the presence of 1 μg/ml cycloheximide (CHX) as confirmed by real-time PCR.

    Journal: Oncoscience

    Article Title: The PI3K regulatory subunit gene PIK3R1 is under direct control of androgens and repressed in prostate cancer cells

    doi:

    Figure Lengend Snippet: The PI3K regulatory subunit genes PIK3R1 and PIK3R3 are direct targets of the AR (A) LNCaP cells were cultured in medium supplemented with 10% dextran charcoal stripped FBS to produce a steroid deplete medium (SD). Following culture for 72 hours, cells were treated with 10 nM synthetic androgen analogue methyltrienolone (R1881) for 24 hours (A+). Relative expression of PIK3R1 , PIK3R3 and PIK3CA was detected by real-time PCR (B) Expression of PIK3R1 , PIK3R3 and PIK3CA mRNA in cells grown in steroid deplete (SD) or androgen (A+) treated conditions over a 24 hours period. The response to androgens was confirmed using PSA (KLK3) expression (not shown). (C) Repression of PIK3R1 and PIK3R3 is also evident in LNCaP cells treated with 0.1 to 100 nM of R1881. (D) The reduction in PIK3R1 and PIK3R3 mRNA expression in response to androgens is still seen in the presence of 1 μg/ml cycloheximide (CHX) as confirmed by real-time PCR.

    Article Snippet: For androgen treatment of LNCaP cells, medium was supplemented with 10% dextran charcoal stripped FBS (PAA Laboratories, A15-119) to produce a steroid-deplete medium.

    Techniques: Cell Culture, Expressing, Real-time Polymerase Chain Reaction

    In vitro adherent cells from decidual tissues. (a) Representative photomicrograph of spindle-shaped adherent cells isolated by α MEM and 10% FBS. (b) Cells isolated in vitro by serum-deprived medium (Quantum 333) at early passages. (c) Another population of cells was in vitro isolated contextually with EDT-MSC. These elements share similarities with endothelial cells forming a monolayer of polygonal, sometimes binucleated cells. Original magnification 100x.

    Journal: BioMed Research International

    Article Title: Isolation, Characterization, and Transduction of Endometrial Decidual Tissue Multipotent Mesenchymal Stromal/Stem Cells from Menstrual Blood

    doi: 10.1155/2013/901821

    Figure Lengend Snippet: In vitro adherent cells from decidual tissues. (a) Representative photomicrograph of spindle-shaped adherent cells isolated by α MEM and 10% FBS. (b) Cells isolated in vitro by serum-deprived medium (Quantum 333) at early passages. (c) Another population of cells was in vitro isolated contextually with EDT-MSC. These elements share similarities with endothelial cells forming a monolayer of polygonal, sometimes binucleated cells. Original magnification 100x.

    Article Snippet: Clonogenic Assay Adherent cells, out of passage 1 (P1) or passage 2 (P2), were seeded at clonal density of 100 cells/cm2 in α MEM (Gibco), 1% L-glutamine (Lonza), 1% penicillin/streptomycin (PAA Laboratories), and 10% FBS (PAA Laboratories).

    Techniques: In Vitro, Isolation

    Dose-dependent effects of celastrol on cellular viability in MCF-7, HepG2, and THP-1. Cells were seeded overnight in 96-well plates at a density of 2 × 10 5 /ml in phenol red-free DMEM supplemented with 5% charcoal absorbed FBS,

    Journal: Cell Stress & Chaperones

    Article Title: Celastrol regulates multiple nuclear transcription factors belonging to HSP90's clients in a dose- and cell type-dependent way

    doi: 10.1007/s12192-010-0202-1

    Figure Lengend Snippet: Dose-dependent effects of celastrol on cellular viability in MCF-7, HepG2, and THP-1. Cells were seeded overnight in 96-well plates at a density of 2 × 10 5 /ml in phenol red-free DMEM supplemented with 5% charcoal absorbed FBS,

    Article Snippet: RPMI 1640 medium, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and streptomycin/penicillin for cell culture use were obtained from PAA Laboratories (Linz, Austria).

    Techniques: