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Millipore fetal bovine serum fbs
Fetal Bovine Serum Fbs, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fetal bovine serum fbs/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fetal bovine serum fbs - by Bioz Stars, 2021-03
86/100 stars

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Related Articles

Cell Adhesion Assay:

Article Title: Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain
Article Snippet: The DMEM serum-free medium was removed, and the Caco-2 cells were incubated with the bacterial inoculum for 1 h in 5% (vol/vol) CO2 at 37°C. .. For the adhesion assay, after infection, the Caco-2 cells were washed four times with PBS before disruption with a 1-ml volume of PBS containing 1% (vol/vol) Triton X-100 (Sigma-Aldrich). ..

Infection:

Article Title: Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain
Article Snippet: The DMEM serum-free medium was removed, and the Caco-2 cells were incubated with the bacterial inoculum for 1 h in 5% (vol/vol) CO2 at 37°C. .. For the adhesion assay, after infection, the Caco-2 cells were washed four times with PBS before disruption with a 1-ml volume of PBS containing 1% (vol/vol) Triton X-100 (Sigma-Aldrich). ..

Cell Culture:

Article Title: TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells
Article Snippet: .. To determine the impact of specific supplements on cardiomyogenic differentiation, cells were cultured in BM alone and BM supplemented with insulin (10 µg/ ml [ ]; Sigma), oxytocin (0.1 µM [ ]; Becham California), bFGF (20 ng/ml [ , ]; R & D Research), or TGF-β1 (2.5 ng/ml [ ]; R & D Research). ..

Mouse Assay:

Article Title: Inhibition of astrocyte FAK-JNK signaling promotes subventricular zone neurogenesis through CNTF
Article Snippet: A 0.5 mm wide strip of the periventricular region, which includes the SVZ and part of the medial striatum, from the genu of the corpus callosum to the anterior commissure decussation was dissected out, flash frozen in liquid nitrogen, and stored at −80 °C for mRNA and protein analysis. .. In order to label proliferating cells in the SVZ in the FAK14 experiments, mice received daily BrdU (i.p., 50 mg/kg, Sigma-Aldrich, B5002) or 5-ethynyl-2’-deoxyuridine (EdU, i.p., 50 mg/kg, Molecular Probes, ) injections 4 h following the FAK14 injections and perfused with ice cold PBS and 4% PFA 2 h after last BrdU or EdU injection. .. For the BrdU pulse-chase study to identify neural stem cell proliferation, mice received daily PBS or FAK14 followed by BrdU 4 h later, for 3 days.

Injection:

Article Title: Inhibition of astrocyte FAK-JNK signaling promotes subventricular zone neurogenesis through CNTF
Article Snippet: A 0.5 mm wide strip of the periventricular region, which includes the SVZ and part of the medial striatum, from the genu of the corpus callosum to the anterior commissure decussation was dissected out, flash frozen in liquid nitrogen, and stored at −80 °C for mRNA and protein analysis. .. In order to label proliferating cells in the SVZ in the FAK14 experiments, mice received daily BrdU (i.p., 50 mg/kg, Sigma-Aldrich, B5002) or 5-ethynyl-2’-deoxyuridine (EdU, i.p., 50 mg/kg, Molecular Probes, ) injections 4 h following the FAK14 injections and perfused with ice cold PBS and 4% PFA 2 h after last BrdU or EdU injection. .. For the BrdU pulse-chase study to identify neural stem cell proliferation, mice received daily PBS or FAK14 followed by BrdU 4 h later, for 3 days.

Expressing:

Article Title: Physiological CRAC channel activation and pore properties require STIM1 binding to all six Orai1 subunits
Article Snippet: FRET microscopy Prior to imaging, cells were trypsinized into suspension and plated for 30–60 min at 37°C. .. Cells expressing full-length STIM1 were store-depleted with 1 µM thapsigargin (EMD Millipore) in 0 mM Ca2+ Ringer’s for 10 min before imaging, whereas cells expressing soluble CAD or SOAR constructs were imaged in 2 mM Ca2+ Ringer’s. .. Experiments were performed on a Zeiss Axiovert 200M microscope (Zeiss) controlled by µManager software, using a Polychrome II xenon light source for illumination (TILL Photonics) and 40× Fluar oil-immersion objective (NA 1.3).

Imaging:

Article Title: Physiological CRAC channel activation and pore properties require STIM1 binding to all six Orai1 subunits
Article Snippet: FRET microscopy Prior to imaging, cells were trypsinized into suspension and plated for 30–60 min at 37°C. .. Cells expressing full-length STIM1 were store-depleted with 1 µM thapsigargin (EMD Millipore) in 0 mM Ca2+ Ringer’s for 10 min before imaging, whereas cells expressing soluble CAD or SOAR constructs were imaged in 2 mM Ca2+ Ringer’s. .. Experiments were performed on a Zeiss Axiovert 200M microscope (Zeiss) controlled by µManager software, using a Polychrome II xenon light source for illumination (TILL Photonics) and 40× Fluar oil-immersion objective (NA 1.3).

Construct:

Article Title: Physiological CRAC channel activation and pore properties require STIM1 binding to all six Orai1 subunits
Article Snippet: FRET microscopy Prior to imaging, cells were trypsinized into suspension and plated for 30–60 min at 37°C. .. Cells expressing full-length STIM1 were store-depleted with 1 µM thapsigargin (EMD Millipore) in 0 mM Ca2+ Ringer’s for 10 min before imaging, whereas cells expressing soluble CAD or SOAR constructs were imaged in 2 mM Ca2+ Ringer’s. .. Experiments were performed on a Zeiss Axiovert 200M microscope (Zeiss) controlled by µManager software, using a Polychrome II xenon light source for illumination (TILL Photonics) and 40× Fluar oil-immersion objective (NA 1.3).

other:

Article Title: Derivation of Neural Stem Cells from Mesenchymal Stem Cells: Evidence for a Bipotential Stem Cell Population
Article Snippet: Human fetal brain-derived astrocytes were plated onto 24-well plates, precoated with 0.1% gelatin (Sigma) at 4°C overnight, at a density of 10,000 cells per well in the coculture medium.

Article Title: Cortical and trabecular bone are equally affected in rats with renal failure and secondary hyperparathyroidism
Article Snippet: Our study investigated the negative effects of renal failure and its relationship with phosphorus, FGF23, PTH, sclerostin, and bone.

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    Millipore hct116 fetal bovine serum
    Lipid peroxidation of the <t>HCT116</t> cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using one-way ANOVA with Bonferroni's post-hoc test: *p
    Hct116 Fetal Bovine Serum, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hct116 fetal bovine serum/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hct116 fetal bovine serum - by Bioz Stars, 2021-03
    98/100 stars
      Buy from Supplier

    Image Search Results


    Lipid peroxidation of the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using one-way ANOVA with Bonferroni's post-hoc test: *p

    Journal: Journal of Cancer

    Article Title: Iron Chelators and Exogenic Photosensitizers. Synergy through Oxidative Stress Gene Expression

    doi: 10.7150/jca.17959

    Figure Lengend Snippet: Lipid peroxidation of the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using one-way ANOVA with Bonferroni's post-hoc test: *p

    Article Snippet: The cells were grown as monolayer cultures in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 12% (HCT116) Fetal Bovine Serum (Sigma) and standard antibiotics in 75 cm2 flasks (Nunc).

    Techniques: Incubation

    mRNA expression of MnSOD and CAT in the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using the Student's t-test: *p

    Journal: Journal of Cancer

    Article Title: Iron Chelators and Exogenic Photosensitizers. Synergy through Oxidative Stress Gene Expression

    doi: 10.7150/jca.17959

    Figure Lengend Snippet: mRNA expression of MnSOD and CAT in the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using the Student's t-test: *p

    Article Snippet: The cells were grown as monolayer cultures in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 12% (HCT116) Fetal Bovine Serum (Sigma) and standard antibiotics in 75 cm2 flasks (Nunc).

    Techniques: Expressing, Incubation

    Cellular localization of TSCs; MS168 (a), Dp44mT (b), 3-AP (c) and PSs; chlorin c (d), Foscan (e) in the HCT116 cell line. First panel - fluorescence of the investigated compounds alone, middle panel - colocalization with mitochondria marker (MitoTracker® Orange for TSC's and Green for PS's), last panel - merge. Scale bar represents 50 μm.

    Journal: Journal of Cancer

    Article Title: Iron Chelators and Exogenic Photosensitizers. Synergy through Oxidative Stress Gene Expression

    doi: 10.7150/jca.17959

    Figure Lengend Snippet: Cellular localization of TSCs; MS168 (a), Dp44mT (b), 3-AP (c) and PSs; chlorin c (d), Foscan (e) in the HCT116 cell line. First panel - fluorescence of the investigated compounds alone, middle panel - colocalization with mitochondria marker (MitoTracker® Orange for TSC's and Green for PS's), last panel - merge. Scale bar represents 50 μm.

    Article Snippet: The cells were grown as monolayer cultures in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 12% (HCT116) Fetal Bovine Serum (Sigma) and standard antibiotics in 75 cm2 flasks (Nunc).

    Techniques: Fluorescence, Marker

    Expression of pancreatic endocrine genes in islet-derived cells cultured with different media. Analysis of gene expression (mRNA) using qRT-PCR was performed on cells isolated from different pigs and representative results are presented. (a) Islet-derived cells were cultured in CMRL media containing 10% FBS (CF) or 20% PS (CP) and in DMEM media containing 10% FBS (DF) or 20% PS (DP) and analyzed for the expression of insulin mRNA. (b–d) Islet-derived cells were cultured in DMEM media containing 10% FBS and 1 g/L (L) or 4.5 g/L (H) glucose and analyzed for the expression of insulin (b), glucagon (c), and somatostatin (d) mRNA. The results are the average fold increase for each indicated mRNA compared with the level of this mRNA in adipose-derived stem cells (ADSC) and normalized for the expression of GAPDH.

    Journal: Journal of Diabetes Research

    Article Title: In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications

    doi: 10.1155/2016/5807876

    Figure Lengend Snippet: Expression of pancreatic endocrine genes in islet-derived cells cultured with different media. Analysis of gene expression (mRNA) using qRT-PCR was performed on cells isolated from different pigs and representative results are presented. (a) Islet-derived cells were cultured in CMRL media containing 10% FBS (CF) or 20% PS (CP) and in DMEM media containing 10% FBS (DF) or 20% PS (DP) and analyzed for the expression of insulin mRNA. (b–d) Islet-derived cells were cultured in DMEM media containing 10% FBS and 1 g/L (L) or 4.5 g/L (H) glucose and analyzed for the expression of insulin (b), glucagon (c), and somatostatin (d) mRNA. The results are the average fold increase for each indicated mRNA compared with the level of this mRNA in adipose-derived stem cells (ADSC) and normalized for the expression of GAPDH.

    Article Snippet: In this study, two types of basal media were applied, namely, Connaught Medical Research Laboratories Medium (CMRL-1066) (Sigma) and Dulbecco's Modified Eagle Medium (DMEM) (HyClone Laboratories), supplied with 10% fetal bovine serum (FBS) (Sigma) or 20% porcine serum (PS) (Sigma), 1% penicillin-streptomycin (HyClone Laboratories), and 4.0 μ M L-glutamine (as shown in ).

    Techniques: Expressing, Derivative Assay, Cell Culture, Quantitative RT-PCR, Isolation

    Islet-derived cell proliferation in culture. The doubling time (hours) of islet-derived cells was measured in CMRL and DMEM media supplemented with 10% fetal bovine serum (FBS) and 20% porcine serum (PS) at the indicated passages.

    Journal: Journal of Diabetes Research

    Article Title: In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications

    doi: 10.1155/2016/5807876

    Figure Lengend Snippet: Islet-derived cell proliferation in culture. The doubling time (hours) of islet-derived cells was measured in CMRL and DMEM media supplemented with 10% fetal bovine serum (FBS) and 20% porcine serum (PS) at the indicated passages.

    Article Snippet: In this study, two types of basal media were applied, namely, Connaught Medical Research Laboratories Medium (CMRL-1066) (Sigma) and Dulbecco's Modified Eagle Medium (DMEM) (HyClone Laboratories), supplied with 10% fetal bovine serum (FBS) (Sigma) or 20% porcine serum (PS) (Sigma), 1% penicillin-streptomycin (HyClone Laboratories), and 4.0 μ M L-glutamine (as shown in ).

    Techniques: Derivative Assay

    Morphological evaluation of DD29 cells. (a) Confocal imaging of ZO-1 and P-cadherin in the DD29 cells and PN day10-mouse RPE. The polarized expression pattern of ZO-1 on the apical side of the cells is shown (black arrows). Nuclei were counter-stained with DAPI. (b) Electron microscopy image of the DD29 cells. Magnified views of the areas of the yellow dotted squares show the presence of microvilli (c), intercellular attachment (d), and extracellular matrix (e). (f) Immunocytochemistry of ZO-1 of DD29 cells maintained by MEM / N1 / FBS medium and serum-free RPE medium (SFRM). Most cells maintained by MEM / N1 / FBS medium were in a cuboidal shape and formed a monolayer structure. ZO-1-positive cell clumps (arrows) in a different plane of Z-scan imaging with increased cell sizes was observed when cells were maintained with SFRM. Scale bars: 30um (f), 20 μm (a), 10 μm (b), 2 μm (c) and 0.5 μm (d and e).

    Journal: PLoS ONE

    Article Title: Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool

    doi: 10.1371/journal.pone.0158282

    Figure Lengend Snippet: Morphological evaluation of DD29 cells. (a) Confocal imaging of ZO-1 and P-cadherin in the DD29 cells and PN day10-mouse RPE. The polarized expression pattern of ZO-1 on the apical side of the cells is shown (black arrows). Nuclei were counter-stained with DAPI. (b) Electron microscopy image of the DD29 cells. Magnified views of the areas of the yellow dotted squares show the presence of microvilli (c), intercellular attachment (d), and extracellular matrix (e). (f) Immunocytochemistry of ZO-1 of DD29 cells maintained by MEM / N1 / FBS medium and serum-free RPE medium (SFRM). Most cells maintained by MEM / N1 / FBS medium were in a cuboidal shape and formed a monolayer structure. ZO-1-positive cell clumps (arrows) in a different plane of Z-scan imaging with increased cell sizes was observed when cells were maintained with SFRM. Scale bars: 30um (f), 20 μm (a), 10 μm (b), 2 μm (c) and 0.5 μm (d and e).

    Article Snippet: At DD16, medium was changed to MEM / N1 / FBS medium (MEM-alpha (M-4526, Sigma) / 1% FBS / 1% N1 supplement (N-6530, Sigma) / 2 mM L-Glutamine (G7513, Sigma) / 0.1 mM NEAA / 250 mg/L L-taurine (T8691, Sigma) / 20 μg/L hydrocortisone (H-0396, Sigma) / 0.013 μg/L triiodo-thyronine (T-5516, Sigma)) which was used for human fetal RPE culture [ ].

    Techniques: Imaging, Expressing, Staining, Electron Microscopy, Immunocytochemistry

    Two-dimensional interactive document mapping (IDMAP) plot for the capacitance spectra for uncoated electrodes and 3.5 CHI/HA-multilayer functionalized electrodes exposed to different tumor cell concentrations (50–1500 cells/µL) and non-specific analytes (glucose (100 mg/dL), ascorbic acid (4.8 mg/mL), and fetal bovine serum (FBS)).

    Journal: Cells

    Article Title: Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions

    doi: 10.3390/cells9061563

    Figure Lengend Snippet: Two-dimensional interactive document mapping (IDMAP) plot for the capacitance spectra for uncoated electrodes and 3.5 CHI/HA-multilayer functionalized electrodes exposed to different tumor cell concentrations (50–1500 cells/µL) and non-specific analytes (glucose (100 mg/dL), ascorbic acid (4.8 mg/mL), and fetal bovine serum (FBS)).

    Article Snippet: Materials Hyaluronic acid (HA, ~1500–1800 kDa) extracted from Streptococcus equi , low molecular weight chitosan (CHI) (deacetylation degree > 85%), polyethyleneimine (PEI, MW ~7.5 KDa), fetal bovine serum (FBS), Dulbecco’s phosphate-buffered saline (DPBS), and phosphate-buffered saline (PBS) were purchased from Sigma Aldrich, Saint Louis, USA.

    Techniques: