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Mediatech fetal bovine serum fbs
Effect of cocaine on cell cycle after 24 h. The glial cells with starting density of 1.3 × 10 6 per dish in complete <t>RPMI</t> 1640 medium containing 10% <t>FBS</t> were treated with 0, 3, 4 and 5 mM of cocaine for 24 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 5, * P
Fetal Bovine Serum Fbs, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Cocaine Induces Alterations in Mitochondrial Membrane Potential and Dual Cell Cycle Arrest in Rat C6 Astroglioma Cells"

Article Title: Cocaine Induces Alterations in Mitochondrial Membrane Potential and Dual Cell Cycle Arrest in Rat C6 Astroglioma Cells

Journal: Neurochemical research

doi: 10.1007/s11064-009-0053-2

Effect of cocaine on cell cycle after 24 h. The glial cells with starting density of 1.3 × 10 6 per dish in complete RPMI 1640 medium containing 10% FBS were treated with 0, 3, 4 and 5 mM of cocaine for 24 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 5, * P
Figure Legend Snippet: Effect of cocaine on cell cycle after 24 h. The glial cells with starting density of 1.3 × 10 6 per dish in complete RPMI 1640 medium containing 10% FBS were treated with 0, 3, 4 and 5 mM of cocaine for 24 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 5, * P

Techniques Used: Staining, Flow Cytometry, Cytometry

Population doubling period of rat glial cells. Cells with starting density of 5 × 10 3 per well in 96-well plates ( n = 4, and each value is the average absorbance of eight wells, so final n at each time point = 32) with complete RPMI 1640 medium containing 10% FBS were incubated for designated time points. The cells were stained with crystal violet and the absorbance at 540 nm was read in a plate reader. The absorbance values at any time point were so close to each other that error bars were not seen on the plot
Figure Legend Snippet: Population doubling period of rat glial cells. Cells with starting density of 5 × 10 3 per well in 96-well plates ( n = 4, and each value is the average absorbance of eight wells, so final n at each time point = 32) with complete RPMI 1640 medium containing 10% FBS were incubated for designated time points. The cells were stained with crystal violet and the absorbance at 540 nm was read in a plate reader. The absorbance values at any time point were so close to each other that error bars were not seen on the plot

Techniques Used: Incubation, Staining

Effect of cocaine on glial cell viability. The cells (2 × 10 4 per well) were seeded in 96 well plates with complete RPMI 1640 medium containing 10% FBS and were treated with various concentrations of cocaine for 24 or 48 h. Data were represented as mean ± SEM ( n = 36, * P
Figure Legend Snippet: Effect of cocaine on glial cell viability. The cells (2 × 10 4 per well) were seeded in 96 well plates with complete RPMI 1640 medium containing 10% FBS and were treated with various concentrations of cocaine for 24 or 48 h. Data were represented as mean ± SEM ( n = 36, * P

Techniques Used:

Effect of cocaine on glial cell cycle after 48 h. The glial cells with starting density of 0.7 × 10 6 per dish in complete RPMI 1640 medium containing 10% FBS were treated with 0, 3, 4 and 5 mM of cocaine for 48 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 9, * P
Figure Legend Snippet: Effect of cocaine on glial cell cycle after 48 h. The glial cells with starting density of 0.7 × 10 6 per dish in complete RPMI 1640 medium containing 10% FBS were treated with 0, 3, 4 and 5 mM of cocaine for 48 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 9, * P

Techniques Used: Staining, Flow Cytometry, Cytometry

Effect of cocaine on mitochondrial membrane potential. The glial cells (2 × 10 4 per well) were seeded in 96 well plates with complete RPMI 1640 media containing 10% FBS and were treated with various concentrations of cocaine (2–6 mM) for 24 h. Membrane potential was evaluated fluorometrically as a measure of rhodamine- 123 uptake. Data were represented as mean ± SEM ( n = 12, * P
Figure Legend Snippet: Effect of cocaine on mitochondrial membrane potential. The glial cells (2 × 10 4 per well) were seeded in 96 well plates with complete RPMI 1640 media containing 10% FBS and were treated with various concentrations of cocaine (2–6 mM) for 24 h. Membrane potential was evaluated fluorometrically as a measure of rhodamine- 123 uptake. Data were represented as mean ± SEM ( n = 12, * P

Techniques Used:

2) Product Images from "Deptor Knockdown Enhances mTOR Activity and Protein Synthesis in Myocytes and Ameliorates Disuse Muscle Atrophy"

Article Title: Deptor Knockdown Enhances mTOR Activity and Protein Synthesis in Myocytes and Ameliorates Disuse Muscle Atrophy

Journal: Molecular Medicine

doi: 10.2119/molmed.2011.00070

Effect of Deptor KD on cell cycle regulation. Control and Deptor KD myoblasts were grown in DMEM supplemented with 10% FBS overnight to 50–60% confluency. Myoblasts were then serum starved for 18–24 h in serum-free DMEM to arrest them
Figure Legend Snippet: Effect of Deptor KD on cell cycle regulation. Control and Deptor KD myoblasts were grown in DMEM supplemented with 10% FBS overnight to 50–60% confluency. Myoblasts were then serum starved for 18–24 h in serum-free DMEM to arrest them

Techniques Used:

Effect of Deptor KD on cell cycle in C2C12 myoblasts. Myoblasts were transfected with either control (scramble) shRNA or shRNA targeting Deptor. Myoblasts were grown in DMEM supplemented with 10% FBS for 18–24 h and stained with propidium iodide
Figure Legend Snippet: Effect of Deptor KD on cell cycle in C2C12 myoblasts. Myoblasts were transfected with either control (scramble) shRNA or shRNA targeting Deptor. Myoblasts were grown in DMEM supplemented with 10% FBS for 18–24 h and stained with propidium iodide

Techniques Used: Transfection, shRNA, Staining

3) Product Images from "Bufadienolides from Venenum Bufonis Inhibit mTOR-Mediated Cyclin D1 and Retinoblastoma Protein Leading to Arrest of Cell Cycle in Cancer Cells"

Article Title: Bufadienolides from Venenum Bufonis Inhibit mTOR-Mediated Cyclin D1 and Retinoblastoma Protein Leading to Arrest of Cell Cycle in Cancer Cells

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2018/3247402

Bufadienolides inhibit cancer cell proliferation. Bufadienolides inhibit cancer cell proliferation in a dose-dependent way. (a) MCF-7 and DU-145 cells, grown in 96-well plates with 10% FBS-DMEM medium, were treated with bufadienolides (0-20 μ g/ml) for 48 h. Cell proliferation was assessed using one solution reagent (Promega). Absorbance at 490 nm was determined by a Biotek Multilabel Counter. (b) The representative photograph was taken by ZEISS microscope (× 200).
Figure Legend Snippet: Bufadienolides inhibit cancer cell proliferation. Bufadienolides inhibit cancer cell proliferation in a dose-dependent way. (a) MCF-7 and DU-145 cells, grown in 96-well plates with 10% FBS-DMEM medium, were treated with bufadienolides (0-20 μ g/ml) for 48 h. Cell proliferation was assessed using one solution reagent (Promega). Absorbance at 490 nm was determined by a Biotek Multilabel Counter. (b) The representative photograph was taken by ZEISS microscope (× 200).

Techniques Used: Microscopy

4) Product Images from "Isolation and Differentiation of Mesenchymal Stem Cells From Broiler Chicken Compact Bones"

Article Title: Isolation and Differentiation of Mesenchymal Stem Cells From Broiler Chicken Compact Bones

Journal: Frontiers in Physiology

doi: 10.3389/fphys.2018.01892

Multilineage differentiation potential of cBMSCs. On confluency, cBMSCs were treated with osteogenic media (OM) containing DMEM with 10 −7 M dexamethasone (DXA) (Sigma Aldrich, MO, USA), 10 mM β-glycerophosphate (Sigma Aldrich, MO, USA), 50 μg/ml ascorbate (Sigma Aldrich, MO, USA), and 5% FBS for osteogenic induction. Cells cultured in DMEM basal media with 10% FBS were used as negative control. Both cells were stained with Alizarin red stain and Von Kossa stain at day 14. Alizarin Red stain in (A) control cells (B) OM treated cells. Von Kossa stain in (C) control cells and (D) OM treated cells. Alkaline Phosphate assay in (E) control cells and (F) OM treated cells.
Figure Legend Snippet: Multilineage differentiation potential of cBMSCs. On confluency, cBMSCs were treated with osteogenic media (OM) containing DMEM with 10 −7 M dexamethasone (DXA) (Sigma Aldrich, MO, USA), 10 mM β-glycerophosphate (Sigma Aldrich, MO, USA), 50 μg/ml ascorbate (Sigma Aldrich, MO, USA), and 5% FBS for osteogenic induction. Cells cultured in DMEM basal media with 10% FBS were used as negative control. Both cells were stained with Alizarin red stain and Von Kossa stain at day 14. Alizarin Red stain in (A) control cells (B) OM treated cells. Von Kossa stain in (C) control cells and (D) OM treated cells. Alkaline Phosphate assay in (E) control cells and (F) OM treated cells.

Techniques Used: Cell Culture, Negative Control, Staining

Comparative analysis of lineage differentiation specific gene expression in cBMSc. Relative gene expression of osteogenic, adipogenic, and myogenic markers were analyzed between induced cells and control cells (C) using qRT-PCR. (A) BSP, Collagen Type 1 Alpha 2 (Col 1A2), BMP2, and BGLAP mRNA expression were analyzed in cells treated with osteogenic media (OM) (containing DMEM with 10 −7 M dexamethasone (DXA), 10 mM β-glycerophosphate, 50 μg/ml ascorbate, and 5% FBS) and control media (C) for 72 h; (B) FABP2, PPARγ, c/EBPα, and c/EBPβ expression were analyzed in cells treated with adipogenic media (AM) (containing 500 nM dexamethasone, 0.5 μM 3-isobutyl-1-methylxanthine, and 20 mg/mL insulin and 300 μM OA) and control media (C) for 48 h (C) MyoD, Myogenin, Myf5, and Pax7 mRNA expression were analyzed in cells treated with myogenic media (MM) (containing DMEM, 5% horse serum, 50 μM hydrocortisone, and 0.1 μM dexamethasone) and control media (C) for 72 h. GAPDH was used as a housekeeping gene. Different letters (a,b) indicate significant difference at P
Figure Legend Snippet: Comparative analysis of lineage differentiation specific gene expression in cBMSc. Relative gene expression of osteogenic, adipogenic, and myogenic markers were analyzed between induced cells and control cells (C) using qRT-PCR. (A) BSP, Collagen Type 1 Alpha 2 (Col 1A2), BMP2, and BGLAP mRNA expression were analyzed in cells treated with osteogenic media (OM) (containing DMEM with 10 −7 M dexamethasone (DXA), 10 mM β-glycerophosphate, 50 μg/ml ascorbate, and 5% FBS) and control media (C) for 72 h; (B) FABP2, PPARγ, c/EBPα, and c/EBPβ expression were analyzed in cells treated with adipogenic media (AM) (containing 500 nM dexamethasone, 0.5 μM 3-isobutyl-1-methylxanthine, and 20 mg/mL insulin and 300 μM OA) and control media (C) for 48 h (C) MyoD, Myogenin, Myf5, and Pax7 mRNA expression were analyzed in cells treated with myogenic media (MM) (containing DMEM, 5% horse serum, 50 μM hydrocortisone, and 0.1 μM dexamethasone) and control media (C) for 72 h. GAPDH was used as a housekeeping gene. Different letters (a,b) indicate significant difference at P

Techniques Used: Expressing, Quantitative RT-PCR

Isolation procedure of MSCs from compact bones of chick (cBMSCs). (A) Chicks were soaked in alcohol for leg dissections (B) and legs dissected and soaked in DMEM with 10% FBS. (C) Muscles were separated to obtain femur and tibia. (D) Epiphysis was dissected, and bone marrow was flushed with PBS containing 2% FBS. (E) After washing, the bones appeared white in color. (F) Bones were chopped in 1–3 mm 3 and digested in digestion media.
Figure Legend Snippet: Isolation procedure of MSCs from compact bones of chick (cBMSCs). (A) Chicks were soaked in alcohol for leg dissections (B) and legs dissected and soaked in DMEM with 10% FBS. (C) Muscles were separated to obtain femur and tibia. (D) Epiphysis was dissected, and bone marrow was flushed with PBS containing 2% FBS. (E) After washing, the bones appeared white in color. (F) Bones were chopped in 1–3 mm 3 and digested in digestion media.

Techniques Used: Isolation

5) Product Images from "Bufadienolides from Venenum Bufonis Inhibit mTOR-Mediated Cyclin D1 and Retinoblastoma Protein Leading to Arrest of Cell Cycle in Cancer Cells"

Article Title: Bufadienolides from Venenum Bufonis Inhibit mTOR-Mediated Cyclin D1 and Retinoblastoma Protein Leading to Arrest of Cell Cycle in Cancer Cells

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2018/3247402

Bufadienolides inhibit cancer cell proliferation. Bufadienolides inhibit cancer cell proliferation in a dose-dependent way. (a) MCF-7 and DU-145 cells, grown in 96-well plates with 10% FBS-DMEM medium, were treated with bufadienolides (0-20 μ g/ml) for 48 h. Cell proliferation was assessed using one solution reagent (Promega). Absorbance at 490 nm was determined by a Biotek Multilabel Counter. (b) The representative photograph was taken by ZEISS microscope (× 200).
Figure Legend Snippet: Bufadienolides inhibit cancer cell proliferation. Bufadienolides inhibit cancer cell proliferation in a dose-dependent way. (a) MCF-7 and DU-145 cells, grown in 96-well plates with 10% FBS-DMEM medium, were treated with bufadienolides (0-20 μ g/ml) for 48 h. Cell proliferation was assessed using one solution reagent (Promega). Absorbance at 490 nm was determined by a Biotek Multilabel Counter. (b) The representative photograph was taken by ZEISS microscope (× 200).

Techniques Used: Microscopy

6) Product Images from "Attenuating effect of N-acetyl-L-cysteine against acute cocaine toxicity in rat C6 astroglial cells"

Article Title: Attenuating effect of N-acetyl-L-cysteine against acute cocaine toxicity in rat C6 astroglial cells

Journal: International Journal of Molecular Medicine

doi: 10.3892/ijmm.2013.1391

(A) Nitric oxide production in cocaine-treated astroglial cells. The cells were seeded in 96-well plates with complete RPMI-1640 media lacking phenol red, containing 10% FBS and treated with 2, 3 and 4 mM cocaine for 1 h. Nitric oxide (NO) was detected with Griess reagent. Data are presented as means ± SEM (n=12, * P > 0.05, insignificant in comparison to the control). (B) Standard curve of sodium nitrite (25–400 μM).
Figure Legend Snippet: (A) Nitric oxide production in cocaine-treated astroglial cells. The cells were seeded in 96-well plates with complete RPMI-1640 media lacking phenol red, containing 10% FBS and treated with 2, 3 and 4 mM cocaine for 1 h. Nitric oxide (NO) was detected with Griess reagent. Data are presented as means ± SEM (n=12, * P > 0.05, insignificant in comparison to the control). (B) Standard curve of sodium nitrite (25–400 μM).

Techniques Used:

7) Product Images from "High-level secretion of native recombinant human calreticulin in yeast"

Article Title: High-level secretion of native recombinant human calreticulin in yeast

Journal: Microbial Cell Factories

doi: 10.1186/s12934-015-0356-8

Cellular proliferation induced by native and yeast-derived CRTs. Native CRT isolated from human placenta, and recombinant human CRT purified from yeast P. pastoris and S. cerevisiae were incubated with human fibroblasts and the assay performed as described in “ Methods ”. Cellular proliferation was compared to the untreated control of cells incubated in 0.5 % FBS medium without CRT. Experiments were performed in triplicate (n = 6) and error bars represent standard error of the mean (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001; Dunnett’s test for statistical significance)
Figure Legend Snippet: Cellular proliferation induced by native and yeast-derived CRTs. Native CRT isolated from human placenta, and recombinant human CRT purified from yeast P. pastoris and S. cerevisiae were incubated with human fibroblasts and the assay performed as described in “ Methods ”. Cellular proliferation was compared to the untreated control of cells incubated in 0.5 % FBS medium without CRT. Experiments were performed in triplicate (n = 6) and error bars represent standard error of the mean (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001; Dunnett’s test for statistical significance)

Techniques Used: Derivative Assay, Isolation, Recombinant, Purification, Incubation

8) Product Images from "Transcriptome analysis of hen preadipocytes treated with an adipogenic cocktail (DMIOA) with or without 20(S)-hydroxylcholesterol"

Article Title: Transcriptome analysis of hen preadipocytes treated with an adipogenic cocktail (DMIOA) with or without 20(S)-hydroxylcholesterol

Journal: BMC Genomics

doi: 10.1186/s12864-015-1231-z

Representative images of non-treated preadipocytes (1A), preadipocytes treated with DMIOA (1B), showing remarkable lipid accumulation in preadipocytes treated with DMIOA and relative expressions of FABP4 and C/EBPB in control and DMIOA treated cells (1C). Preadipocytes were cultured in Dulbecco’s modified eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) for 96 hr. Images were taken using an EVOS® xl core cell culture microscope (Advanced Microscopy Group, Seattle, USA) at 20X magnification. Green arrows indicate lipid droplets stained with Oil red O stain whereas there is no lipid formation in non-treated cells. C: Fold change expression of FABP4 and C/EBPβ in preadipocytes treated with an adipogenic cocktail (DMIOA) compared with non-treated cells. Preadipocytes were cultured in Dulbecco’s modified eagle’s medium containing 10% Fetal Bovine Serum for 96 hr. Bars with different letters are significantly different (P
Figure Legend Snippet: Representative images of non-treated preadipocytes (1A), preadipocytes treated with DMIOA (1B), showing remarkable lipid accumulation in preadipocytes treated with DMIOA and relative expressions of FABP4 and C/EBPB in control and DMIOA treated cells (1C). Preadipocytes were cultured in Dulbecco’s modified eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) for 96 hr. Images were taken using an EVOS® xl core cell culture microscope (Advanced Microscopy Group, Seattle, USA) at 20X magnification. Green arrows indicate lipid droplets stained with Oil red O stain whereas there is no lipid formation in non-treated cells. C: Fold change expression of FABP4 and C/EBPβ in preadipocytes treated with an adipogenic cocktail (DMIOA) compared with non-treated cells. Preadipocytes were cultured in Dulbecco’s modified eagle’s medium containing 10% Fetal Bovine Serum for 96 hr. Bars with different letters are significantly different (P

Techniques Used: Cell Culture, Modification, Microscopy, Staining, Expressing

9) Product Images from "Isolation and Differentiation of Mesenchymal Stem Cells From Broiler Chicken Compact Bones"

Article Title: Isolation and Differentiation of Mesenchymal Stem Cells From Broiler Chicken Compact Bones

Journal: Frontiers in Physiology

doi: 10.3389/fphys.2018.01892

Multilineage differentiation potential of cBMSCs. On confluency, cBMSCs were treated with osteogenic media (OM) containing DMEM with 10 −7 M dexamethasone (DXA) (Sigma Aldrich, MO, USA), 10 mM β-glycerophosphate (Sigma Aldrich, MO, USA), 50 μg/ml ascorbate (Sigma Aldrich, MO, USA), and 5% FBS for osteogenic induction. Cells cultured in DMEM basal media with 10% FBS were used as negative control. Both cells were stained with Alizarin red stain and Von Kossa stain at day 14. Alizarin Red stain in (A) control cells (B) OM treated cells. Von Kossa stain in (C) control cells and (D) OM treated cells. Alkaline Phosphate assay in (E) control cells and (F) OM treated cells.
Figure Legend Snippet: Multilineage differentiation potential of cBMSCs. On confluency, cBMSCs were treated with osteogenic media (OM) containing DMEM with 10 −7 M dexamethasone (DXA) (Sigma Aldrich, MO, USA), 10 mM β-glycerophosphate (Sigma Aldrich, MO, USA), 50 μg/ml ascorbate (Sigma Aldrich, MO, USA), and 5% FBS for osteogenic induction. Cells cultured in DMEM basal media with 10% FBS were used as negative control. Both cells were stained with Alizarin red stain and Von Kossa stain at day 14. Alizarin Red stain in (A) control cells (B) OM treated cells. Von Kossa stain in (C) control cells and (D) OM treated cells. Alkaline Phosphate assay in (E) control cells and (F) OM treated cells.

Techniques Used: Cell Culture, Negative Control, Staining

Comparative analysis of lineage differentiation specific gene expression in cBMSc. Relative gene expression of osteogenic, adipogenic, and myogenic markers were analyzed between induced cells and control cells (C) using qRT-PCR. (A) BSP, Collagen Type 1 Alpha 2 (Col 1A2), BMP2, and BGLAP mRNA expression were analyzed in cells treated with osteogenic media (OM) (containing DMEM with 10 −7 M dexamethasone (DXA), 10 mM β-glycerophosphate, 50 μg/ml ascorbate, and 5% FBS) and control media (C) for 72 h; (B) FABP2, PPARγ, c/EBPα, and c/EBPβ expression were analyzed in cells treated with adipogenic media (AM) (containing 500 nM dexamethasone, 0.5 μM 3-isobutyl-1-methylxanthine, and 20 mg/mL insulin and 300 μM OA) and control media (C) for 48 h (C) MyoD, Myogenin, Myf5, and Pax7 mRNA expression were analyzed in cells treated with myogenic media (MM) (containing DMEM, 5% horse serum, 50 μM hydrocortisone, and 0.1 μM dexamethasone) and control media (C) for 72 h. GAPDH was used as a housekeeping gene. Different letters (a,b) indicate significant difference at P
Figure Legend Snippet: Comparative analysis of lineage differentiation specific gene expression in cBMSc. Relative gene expression of osteogenic, adipogenic, and myogenic markers were analyzed between induced cells and control cells (C) using qRT-PCR. (A) BSP, Collagen Type 1 Alpha 2 (Col 1A2), BMP2, and BGLAP mRNA expression were analyzed in cells treated with osteogenic media (OM) (containing DMEM with 10 −7 M dexamethasone (DXA), 10 mM β-glycerophosphate, 50 μg/ml ascorbate, and 5% FBS) and control media (C) for 72 h; (B) FABP2, PPARγ, c/EBPα, and c/EBPβ expression were analyzed in cells treated with adipogenic media (AM) (containing 500 nM dexamethasone, 0.5 μM 3-isobutyl-1-methylxanthine, and 20 mg/mL insulin and 300 μM OA) and control media (C) for 48 h (C) MyoD, Myogenin, Myf5, and Pax7 mRNA expression were analyzed in cells treated with myogenic media (MM) (containing DMEM, 5% horse serum, 50 μM hydrocortisone, and 0.1 μM dexamethasone) and control media (C) for 72 h. GAPDH was used as a housekeeping gene. Different letters (a,b) indicate significant difference at P

Techniques Used: Expressing, Quantitative RT-PCR

10) Product Images from "PRAS40 Regulates Protein Synthesis and Cell Cycle in C2C12 Myoblasts"

Article Title: PRAS40 Regulates Protein Synthesis and Cell Cycle in C2C12 Myoblasts

Journal: Molecular Medicine

doi: 10.2119/molmed.2009.00168

Effect of PRAS40 knockdown on C2C12 myoblast cell cycle. Myoblasts were transfected with either control (shScramble) shRNA or shRNA targeting PRAS40. Myoblasts were grown in DMEM supplemented with 10% FBS for 24 h and stained with propidium iodide stain
Figure Legend Snippet: Effect of PRAS40 knockdown on C2C12 myoblast cell cycle. Myoblasts were transfected with either control (shScramble) shRNA or shRNA targeting PRAS40. Myoblasts were grown in DMEM supplemented with 10% FBS for 24 h and stained with propidium iodide stain

Techniques Used: Transfection, shRNA, Staining

11) Product Images from "Biodistribution and Delivery Efficiency of Unmodified Tumor-Derived Exosomes"

Article Title: Biodistribution and Delivery Efficiency of Unmodified Tumor-Derived Exosomes

Journal: Journal of controlled release : official journal of the Controlled Release Society

doi: 10.1016/j.jconrel.2014.12.013

A) Doxorubicin release profile from 4T1 exosomes, PC:Chol liposomes, and SynExoLiposomes. Exosomes and liposomes were incubated in 50% (v/v) FBS/PBS at 37°C for 24 hours. B) Balb/c mice inoculated with 4T1 tumors were randomly divided into six groups. Each group of six mice received one of the following intratumoral injections: Control (PBS), PC:Chol liposomes loaded with 1 mg/kg doxorubicin, free doxorubicin 1 mg/kg, SynExoLiposomes loaded with 1 mg/kg doxorubicin, 4T1 exosomes loaded with 1 mg/kg doxorubicin, and free doxorubicin 5 mg/kg. Treatment was administered on days 7, 11, and 15 (arrows).
Figure Legend Snippet: A) Doxorubicin release profile from 4T1 exosomes, PC:Chol liposomes, and SynExoLiposomes. Exosomes and liposomes were incubated in 50% (v/v) FBS/PBS at 37°C for 24 hours. B) Balb/c mice inoculated with 4T1 tumors were randomly divided into six groups. Each group of six mice received one of the following intratumoral injections: Control (PBS), PC:Chol liposomes loaded with 1 mg/kg doxorubicin, free doxorubicin 1 mg/kg, SynExoLiposomes loaded with 1 mg/kg doxorubicin, 4T1 exosomes loaded with 1 mg/kg doxorubicin, and free doxorubicin 5 mg/kg. Treatment was administered on days 7, 11, and 15 (arrows).

Techniques Used: Incubation, Mouse Assay

Related Articles

Modification:

Article Title: Cocaine Induces Alterations in Mitochondrial Membrane Potential and Dual Cell Cycle Arrest in Rat C6 Astroglioma Cells
Article Snippet: .. RPMI 1640 (modified), fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and l -glutamine were purchased from Media Tech (Herndon, VA, US). .. Crystal violet, rhodamine- 123, l -glutaraldehyde, trypan blue, cocaine–HCl, and EDTA (ethylene diamine tetraacetic acid) were supplied by Sigma Chemical Company (St. Louis, MO, US).

Article Title: Bufadienolides from Venenum Bufonis Inhibit mTOR-Mediated Cyclin D1 and Retinoblastoma Protein Leading to Arrest of Cell Cycle in Cancer Cells
Article Snippet: .. Materials Dulbecco's Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased, respectively, from Mediatech (Herndon, VA, USA) and Gibco (Logan, UT, USA). ..

Article Title: Isolation and Differentiation of Mesenchymal Stem Cells From Broiler Chicken Compact Bones
Article Snippet: .. Dissected legs were kept in Dulbecco's Modified Eagle's medium (DMEM) (Mediatech Inc.,VA, USA) containing 10% Fetal Bovine Serum (FBS) (Mediatech Inc.,VA, USA), 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.292 mg/mL L-glutamine (Thermo Fisher Scientific, MA, USA) until connective tissues and muscles were completely removed. ..

Article Title: Bufadienolides from Venenum Bufonis Inhibit mTOR-Mediated Cyclin D1 and Retinoblastoma Protein Leading to Arrest of Cell Cycle in Cancer Cells
Article Snippet: .. Dulbecco's Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased, respectively, from Mediatech (Herndon, VA, USA) and Gibco (Logan, UT, USA). ..

Article Title: Deptor Knockdown Enhances mTOR Activity and Protein Synthesis in Myocytes and Ameliorates Disuse Muscle Atrophy
Article Snippet: .. C2C12 myoblasts (American Type Culture Collection, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), penicillin (100 IU/mL), streptomycin (100 μg/mL) (all from Mediatech, Herndon, VA, USA) under 5% CO2 at 37°C. .. To assess basal mTOR activity, experiments measuring protein synthesis and the phosphorylation of mTOR substrates were performed using 2% FBS without antibiotics- antimycotics for 8 h. 5-Aminoimidazole-4-carboxamide-1-β- d -ribonucleoside (AICAR; Toronto Research Chemicals, Toronto, Canada), when present, was added at a final concentration of 2 μmol/L.

other:

Article Title: Attenuating effect of N-acetyl-L-cysteine against acute cocaine toxicity in rat C6 astroglial cells
Article Snippet: Materials RPMI-1640, fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and L-glutamine were purchased from Mediatech (Herndon, VA, USA).

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    Mediatech fetal bovine serum fbs
    Effect of cocaine on cell cycle after 24 h. The glial cells with starting density of 1.3 × 10 6 per dish in complete <t>RPMI</t> 1640 medium containing 10% <t>FBS</t> were treated with 0, 3, 4 and 5 mM of cocaine for 24 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 5, * P
    Fetal Bovine Serum Fbs, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of cocaine on cell cycle after 24 h. The glial cells with starting density of 1.3 × 10 6 per dish in complete RPMI 1640 medium containing 10% FBS were treated with 0, 3, 4 and 5 mM of cocaine for 24 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 5, * P

    Journal: Neurochemical research

    Article Title: Cocaine Induces Alterations in Mitochondrial Membrane Potential and Dual Cell Cycle Arrest in Rat C6 Astroglioma Cells

    doi: 10.1007/s11064-009-0053-2

    Figure Lengend Snippet: Effect of cocaine on cell cycle after 24 h. The glial cells with starting density of 1.3 × 10 6 per dish in complete RPMI 1640 medium containing 10% FBS were treated with 0, 3, 4 and 5 mM of cocaine for 24 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 5, * P

    Article Snippet: RPMI 1640 (modified), fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and l -glutamine were purchased from Media Tech (Herndon, VA, US).

    Techniques: Staining, Flow Cytometry, Cytometry

    Population doubling period of rat glial cells. Cells with starting density of 5 × 10 3 per well in 96-well plates ( n = 4, and each value is the average absorbance of eight wells, so final n at each time point = 32) with complete RPMI 1640 medium containing 10% FBS were incubated for designated time points. The cells were stained with crystal violet and the absorbance at 540 nm was read in a plate reader. The absorbance values at any time point were so close to each other that error bars were not seen on the plot

    Journal: Neurochemical research

    Article Title: Cocaine Induces Alterations in Mitochondrial Membrane Potential and Dual Cell Cycle Arrest in Rat C6 Astroglioma Cells

    doi: 10.1007/s11064-009-0053-2

    Figure Lengend Snippet: Population doubling period of rat glial cells. Cells with starting density of 5 × 10 3 per well in 96-well plates ( n = 4, and each value is the average absorbance of eight wells, so final n at each time point = 32) with complete RPMI 1640 medium containing 10% FBS were incubated for designated time points. The cells were stained with crystal violet and the absorbance at 540 nm was read in a plate reader. The absorbance values at any time point were so close to each other that error bars were not seen on the plot

    Article Snippet: RPMI 1640 (modified), fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and l -glutamine were purchased from Media Tech (Herndon, VA, US).

    Techniques: Incubation, Staining

    Effect of cocaine on glial cell viability. The cells (2 × 10 4 per well) were seeded in 96 well plates with complete RPMI 1640 medium containing 10% FBS and were treated with various concentrations of cocaine for 24 or 48 h. Data were represented as mean ± SEM ( n = 36, * P

    Journal: Neurochemical research

    Article Title: Cocaine Induces Alterations in Mitochondrial Membrane Potential and Dual Cell Cycle Arrest in Rat C6 Astroglioma Cells

    doi: 10.1007/s11064-009-0053-2

    Figure Lengend Snippet: Effect of cocaine on glial cell viability. The cells (2 × 10 4 per well) were seeded in 96 well plates with complete RPMI 1640 medium containing 10% FBS and were treated with various concentrations of cocaine for 24 or 48 h. Data were represented as mean ± SEM ( n = 36, * P

    Article Snippet: RPMI 1640 (modified), fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and l -glutamine were purchased from Media Tech (Herndon, VA, US).

    Techniques:

    Effect of cocaine on glial cell cycle after 48 h. The glial cells with starting density of 0.7 × 10 6 per dish in complete RPMI 1640 medium containing 10% FBS were treated with 0, 3, 4 and 5 mM of cocaine for 48 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 9, * P

    Journal: Neurochemical research

    Article Title: Cocaine Induces Alterations in Mitochondrial Membrane Potential and Dual Cell Cycle Arrest in Rat C6 Astroglioma Cells

    doi: 10.1007/s11064-009-0053-2

    Figure Lengend Snippet: Effect of cocaine on glial cell cycle after 48 h. The glial cells with starting density of 0.7 × 10 6 per dish in complete RPMI 1640 medium containing 10% FBS were treated with 0, 3, 4 and 5 mM of cocaine for 48 h. Cells were harvested and stained by propidium iodide staining solution for 1 h in dark and analyzed by flow cytometry. Data were represented as mean ± SEM ( n = 9, * P

    Article Snippet: RPMI 1640 (modified), fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and l -glutamine were purchased from Media Tech (Herndon, VA, US).

    Techniques: Staining, Flow Cytometry, Cytometry

    Effect of cocaine on mitochondrial membrane potential. The glial cells (2 × 10 4 per well) were seeded in 96 well plates with complete RPMI 1640 media containing 10% FBS and were treated with various concentrations of cocaine (2–6 mM) for 24 h. Membrane potential was evaluated fluorometrically as a measure of rhodamine- 123 uptake. Data were represented as mean ± SEM ( n = 12, * P

    Journal: Neurochemical research

    Article Title: Cocaine Induces Alterations in Mitochondrial Membrane Potential and Dual Cell Cycle Arrest in Rat C6 Astroglioma Cells

    doi: 10.1007/s11064-009-0053-2

    Figure Lengend Snippet: Effect of cocaine on mitochondrial membrane potential. The glial cells (2 × 10 4 per well) were seeded in 96 well plates with complete RPMI 1640 media containing 10% FBS and were treated with various concentrations of cocaine (2–6 mM) for 24 h. Membrane potential was evaluated fluorometrically as a measure of rhodamine- 123 uptake. Data were represented as mean ± SEM ( n = 12, * P

    Article Snippet: RPMI 1640 (modified), fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and l -glutamine were purchased from Media Tech (Herndon, VA, US).

    Techniques:

    Effect of Deptor KD on cell cycle regulation. Control and Deptor KD myoblasts were grown in DMEM supplemented with 10% FBS overnight to 50–60% confluency. Myoblasts were then serum starved for 18–24 h in serum-free DMEM to arrest them

    Journal: Molecular Medicine

    Article Title: Deptor Knockdown Enhances mTOR Activity and Protein Synthesis in Myocytes and Ameliorates Disuse Muscle Atrophy

    doi: 10.2119/molmed.2011.00070

    Figure Lengend Snippet: Effect of Deptor KD on cell cycle regulation. Control and Deptor KD myoblasts were grown in DMEM supplemented with 10% FBS overnight to 50–60% confluency. Myoblasts were then serum starved for 18–24 h in serum-free DMEM to arrest them

    Article Snippet: C2C12 myoblasts (American Type Culture Collection, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), penicillin (100 IU/mL), streptomycin (100 μg/mL) (all from Mediatech, Herndon, VA, USA) under 5% CO2 at 37°C.

    Techniques:

    Effect of Deptor KD on cell cycle in C2C12 myoblasts. Myoblasts were transfected with either control (scramble) shRNA or shRNA targeting Deptor. Myoblasts were grown in DMEM supplemented with 10% FBS for 18–24 h and stained with propidium iodide

    Journal: Molecular Medicine

    Article Title: Deptor Knockdown Enhances mTOR Activity and Protein Synthesis in Myocytes and Ameliorates Disuse Muscle Atrophy

    doi: 10.2119/molmed.2011.00070

    Figure Lengend Snippet: Effect of Deptor KD on cell cycle in C2C12 myoblasts. Myoblasts were transfected with either control (scramble) shRNA or shRNA targeting Deptor. Myoblasts were grown in DMEM supplemented with 10% FBS for 18–24 h and stained with propidium iodide

    Article Snippet: C2C12 myoblasts (American Type Culture Collection, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), penicillin (100 IU/mL), streptomycin (100 μg/mL) (all from Mediatech, Herndon, VA, USA) under 5% CO2 at 37°C.

    Techniques: Transfection, shRNA, Staining

    Differentiation potential of prostate CSCs. a Expression levels of basal, luminal, and neuroendocrine markers in CSCs and parent cells measured by qRT-PCR ( n = 3). Values in the CSCs were normalized to the respective parent cells. b Morphology of PC3 CSCs cultured in sphere medium in non-adherent petri dish (top) and in DMEM plus 10% FBS in cell culture dish (bottom) for 2 weeks. Scale bar, 500 μm. c mRNA levels of basal, luminal, and neuroendocrine markers in CSCs cultured in regular cell culture medium (DMEM + 10% FBS) for 3 and 8 days ( n = 3). Heatmaps represent the relative mRNA level of each marker normalized to the value at day 1. d mRNA levels of basal, luminal, and neuroendocrine markers in CSCs and CSC-derived tumors (left), and in PC3 cells and PC3 cell-derived tumors (right) ( n = 3). Values in the tumor tissues were normalized to the originating cells. IHC of CK18 ( e ) and SYP ( f ) in tumors derived from PC3 cells or CSCs. Quantitation of signal intensity was obtained by Image J ( n = 3). Scale bar: left panels, 200 μm; right panels, 50 μm. ns not significant.

    Journal: Communications Biology

    Article Title: Chemosensitization of prostate cancer stem cells in mice by angiogenin and plexin-B2 inhibitors

    doi: 10.1038/s42003-020-0750-6

    Figure Lengend Snippet: Differentiation potential of prostate CSCs. a Expression levels of basal, luminal, and neuroendocrine markers in CSCs and parent cells measured by qRT-PCR ( n = 3). Values in the CSCs were normalized to the respective parent cells. b Morphology of PC3 CSCs cultured in sphere medium in non-adherent petri dish (top) and in DMEM plus 10% FBS in cell culture dish (bottom) for 2 weeks. Scale bar, 500 μm. c mRNA levels of basal, luminal, and neuroendocrine markers in CSCs cultured in regular cell culture medium (DMEM + 10% FBS) for 3 and 8 days ( n = 3). Heatmaps represent the relative mRNA level of each marker normalized to the value at day 1. d mRNA levels of basal, luminal, and neuroendocrine markers in CSCs and CSC-derived tumors (left), and in PC3 cells and PC3 cell-derived tumors (right) ( n = 3). Values in the tumor tissues were normalized to the originating cells. IHC of CK18 ( e ) and SYP ( f ) in tumors derived from PC3 cells or CSCs. Quantitation of signal intensity was obtained by Image J ( n = 3). Scale bar: left panels, 200 μm; right panels, 50 μm. ns not significant.

    Article Snippet: PC3, DU145, and P19 cells were cultured in DMEM (Sigma-Aldrich) + 10% FBS (Mediatech).

    Techniques: Expressing, Quantitative RT-PCR, Cell Culture, Marker, Derivative Assay, Immunohistochemistry, Quantitation Assay

    Bufadienolides inhibit cancer cell proliferation. Bufadienolides inhibit cancer cell proliferation in a dose-dependent way. (a) MCF-7 and DU-145 cells, grown in 96-well plates with 10% FBS-DMEM medium, were treated with bufadienolides (0-20 μ g/ml) for 48 h. Cell proliferation was assessed using one solution reagent (Promega). Absorbance at 490 nm was determined by a Biotek Multilabel Counter. (b) The representative photograph was taken by ZEISS microscope (× 200).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Bufadienolides from Venenum Bufonis Inhibit mTOR-Mediated Cyclin D1 and Retinoblastoma Protein Leading to Arrest of Cell Cycle in Cancer Cells

    doi: 10.1155/2018/3247402

    Figure Lengend Snippet: Bufadienolides inhibit cancer cell proliferation. Bufadienolides inhibit cancer cell proliferation in a dose-dependent way. (a) MCF-7 and DU-145 cells, grown in 96-well plates with 10% FBS-DMEM medium, were treated with bufadienolides (0-20 μ g/ml) for 48 h. Cell proliferation was assessed using one solution reagent (Promega). Absorbance at 490 nm was determined by a Biotek Multilabel Counter. (b) The representative photograph was taken by ZEISS microscope (× 200).

    Article Snippet: Materials Dulbecco's Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased, respectively, from Mediatech (Herndon, VA, USA) and Gibco (Logan, UT, USA).

    Techniques: Microscopy