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Gemini Bio fetal bovine serum fbs
Fetal Bovine Serum Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fetal bovine serum fbs/product/Gemini Bio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fetal bovine serum fbs - by Bioz Stars, 2021-04
86/100 stars

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Article Title: Hepatocyte growth factor regulates cyclooxygenase-2 expression via ?-catenin, Akt, and p42/p44 MAPK in human bronchial epithelial cells
Article Snippet: Fetal bovine serum (FBS 100–106) was from Gemini Bio-Products (Woodland, CA).

Article Title: Multiplexed detection of serological cancer markers with plasmon-enhanced Raman spectro-immunoassay
Article Snippet: Phosphate buffered saline (10×PBS) solution was purchased from OmniPur (Billerica, MA) and fetal bovine serum (FBS, BenchMarkTM ) was acquired from Gemini Bio-Products (West Sacramento, CA).

Chloramphenicol Acetyltransferase Assay:

Article Title: Development and validation of a standardized ELISA for the detection of soluble Fc-epsilon-RI in human serum
Article Snippet: The goal of this study, therefore, was to develop and validate an ELISA for standardized quantification of sFcεRI in human serum. .. Goat anti-mouse IgG Fc specific (CatM3534-1mL) from Sigma-Aldrich (St. Louis, MO); Anti-human Fc epsilon Receptor I alpha monoclonal antibody clone AER-37 (CRA1, Cat#16-5899-82) from eBioscience (San Diego, CA); Chimeric IgE (cIgE) contains the immunoglobulin heavy chain of human IgE and recognizes the haptens 4-hydroxy-3-nitrophenylacetic acid (NP) and 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) with its murine variable regions. cIgE was derived from Jw 8/5/13 cells as described ( ; ); Goat anti-human IgE horseradish peroxidase (HRP) conjugated antibody (Cat# ) from Invitrogen (Camarillo, CA); Coating buffer (Cat#00-0044-59) from eBioscience, 10 mM phosphate buffer saline (PBS), fetal bovine serum (FBS, Cat100–106) from Gemini Bio-Products (West-Sacramento, CA), Tween-20 (Cat#P7949-500ML) from Sigma-Aldrich, (3, 3′, 5, 5′)-tetramethylenbenzidine (TMB, Cat#T0440-1L) from Sigma-Aldrich, 2N Sulfuric acid (Cat#A300SI-212) from Fisher Scientific (Pittsburgh, PA) .. Immuno 96 MicroWell Solid Plates MaxiSorp (Cat#442404) from Thermo Scientific (Rochester, NY); BIO-TEK ELISA Microplate washer (Cat#8070-01) from TriContinent Scientific (Grass Valley, CA); Spectramax 250 Microplate reader (Cat#BC-MDSMX250) from Molecular Devices (Sunnyvale, CA).

Derivative Assay:

Article Title: Development and validation of a standardized ELISA for the detection of soluble Fc-epsilon-RI in human serum
Article Snippet: The goal of this study, therefore, was to develop and validate an ELISA for standardized quantification of sFcεRI in human serum. .. Goat anti-mouse IgG Fc specific (CatM3534-1mL) from Sigma-Aldrich (St. Louis, MO); Anti-human Fc epsilon Receptor I alpha monoclonal antibody clone AER-37 (CRA1, Cat#16-5899-82) from eBioscience (San Diego, CA); Chimeric IgE (cIgE) contains the immunoglobulin heavy chain of human IgE and recognizes the haptens 4-hydroxy-3-nitrophenylacetic acid (NP) and 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) with its murine variable regions. cIgE was derived from Jw 8/5/13 cells as described ( ; ); Goat anti-human IgE horseradish peroxidase (HRP) conjugated antibody (Cat# ) from Invitrogen (Camarillo, CA); Coating buffer (Cat#00-0044-59) from eBioscience, 10 mM phosphate buffer saline (PBS), fetal bovine serum (FBS, Cat100–106) from Gemini Bio-Products (West-Sacramento, CA), Tween-20 (Cat#P7949-500ML) from Sigma-Aldrich, (3, 3′, 5, 5′)-tetramethylenbenzidine (TMB, Cat#T0440-1L) from Sigma-Aldrich, 2N Sulfuric acid (Cat#A300SI-212) from Fisher Scientific (Pittsburgh, PA) .. Immuno 96 MicroWell Solid Plates MaxiSorp (Cat#442404) from Thermo Scientific (Rochester, NY); BIO-TEK ELISA Microplate washer (Cat#8070-01) from TriContinent Scientific (Grass Valley, CA); Spectramax 250 Microplate reader (Cat#BC-MDSMX250) from Molecular Devices (Sunnyvale, CA).

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  • 86
    Gemini Bio insulin free fbs
    Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% <t>FBS</t> with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 <t>mmol/L.</t> The [ 3 H]-5HT uptake rate
    Insulin Free Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/insulin free fbs/product/Gemini Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    insulin free fbs - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Gemini Bio fetal bovine serum fbs
    <t>Sirt1-cortactin</t> association is regulated by FGFR1/FGF8. A, Subcellular localization of Sirt1 and cortactin in GN11 cells. Cells were assessed at the basal state (10% <t>FBS)</t> in the presence of either FGF8 (+FGF8) or vehicle (−FGF8) at different time points (0, 24, and 48 h). Immunostaining was conducted for Sirt1 (red) and cortactin (green), and 4′,6′-diamino-2-phenylindole (DAPI; (blue) was used as nuclear marker. Colocalized pixels between Sirt1 and cortactin were automatically highlighted as yellow dots by an imaging analysis software (ImageJ; National Institutes of Health). B, Quantitation of cytoplasmic Sirt1 immunoreactivity in GN11 cells. C, Acetylated-cortactin in GN11 cells treated for 24 hours with either FGF8 or vehicle. D, Cortactin acetylation fold enrichment in GN11 cells. Data are shown as mean ± SEM for n = 3 separate experiments. ***, P
    Fetal Bovine Serum Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal bovine serum fbs/product/Gemini Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum fbs - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Gemini Bio freshly made cryopreservation media
    <t>Cryopreservation</t> and storage of PBMC samples. A description of the requirements for long-term storage of the samples from time of collection is provided.
    Freshly Made Cryopreservation Media, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/freshly made cryopreservation media/product/Gemini Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    freshly made cryopreservation media - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

    Journal:

    Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

    doi: 10.1111/j.1471-4159.2007.04469.x

    Figure Lengend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

    Article Snippet: Also, these data demonstrate that there is a 60% increase in overall DNA synthesis of JAR cells if they enter the cell cycle in media supplemented with 25 mmol/L D-glucose and insulin-free FBS ( ).

    Techniques: Cell Culture

    Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

    Journal:

    Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

    doi: 10.1111/j.1471-4159.2007.04469.x

    Figure Lengend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

    Article Snippet: Also, these data demonstrate that there is a 60% increase in overall DNA synthesis of JAR cells if they enter the cell cycle in media supplemented with 25 mmol/L D-glucose and insulin-free FBS ( ).

    Techniques: Concentration Assay, Cell Culture

    Sirt1-cortactin association is regulated by FGFR1/FGF8. A, Subcellular localization of Sirt1 and cortactin in GN11 cells. Cells were assessed at the basal state (10% FBS) in the presence of either FGF8 (+FGF8) or vehicle (−FGF8) at different time points (0, 24, and 48 h). Immunostaining was conducted for Sirt1 (red) and cortactin (green), and 4′,6′-diamino-2-phenylindole (DAPI; (blue) was used as nuclear marker. Colocalized pixels between Sirt1 and cortactin were automatically highlighted as yellow dots by an imaging analysis software (ImageJ; National Institutes of Health). B, Quantitation of cytoplasmic Sirt1 immunoreactivity in GN11 cells. C, Acetylated-cortactin in GN11 cells treated for 24 hours with either FGF8 or vehicle. D, Cortactin acetylation fold enrichment in GN11 cells. Data are shown as mean ± SEM for n = 3 separate experiments. ***, P

    Journal: Molecular Endocrinology

    Article Title: Sirt1-Deficient Mice Have Hypogonadotropic Hypogonadism due to Defective GnRH Neuronal Migration

    doi: 10.1210/me.2014-1228

    Figure Lengend Snippet: Sirt1-cortactin association is regulated by FGFR1/FGF8. A, Subcellular localization of Sirt1 and cortactin in GN11 cells. Cells were assessed at the basal state (10% FBS) in the presence of either FGF8 (+FGF8) or vehicle (−FGF8) at different time points (0, 24, and 48 h). Immunostaining was conducted for Sirt1 (red) and cortactin (green), and 4′,6′-diamino-2-phenylindole (DAPI; (blue) was used as nuclear marker. Colocalized pixels between Sirt1 and cortactin were automatically highlighted as yellow dots by an imaging analysis software (ImageJ; National Institutes of Health). B, Quantitation of cytoplasmic Sirt1 immunoreactivity in GN11 cells. C, Acetylated-cortactin in GN11 cells treated for 24 hours with either FGF8 or vehicle. D, Cortactin acetylation fold enrichment in GN11 cells. Data are shown as mean ± SEM for n = 3 separate experiments. ***, P

    Article Snippet: GN11, human embryonic kidney (HEK) 293T, and immortalized mouse embryonic fibroblasts (3T3) Sirt1 +/+ and Sirt1 −/− cells ( ) were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) (Gemini Bio-Products), 100 U/mL penicillin, and 100 μg/mL streptomycin in a humidified atmosphere containing 5% CO2 at 37°C ( ).

    Techniques: Immunostaining, Marker, Imaging, Software, Quantitation Assay

    Cryopreservation and storage of PBMC samples. A description of the requirements for long-term storage of the samples from time of collection is provided.

    Journal: Journal of immunological methods

    Article Title: Leukopak PBMC Sample Processing for Preparing Quality Control Material to Support Proficiency Testing Programs

    doi: 10.1016/j.jim.2014.05.019

    Figure Lengend Snippet: Cryopreservation and storage of PBMC samples. A description of the requirements for long-term storage of the samples from time of collection is provided.

    Article Snippet: The PMBCs were then suspended in freshly-made cryopreservation media (90% fetal bovine serum [FBS; Gemini Bio-Products, West Sacramento, CA]+ 10% dimethylsulfoxide [DMSO; Sigma-Aldrich, St. Louis, MO]) to a cell concentration of 20×106 /mL.

    Techniques: