Structured Review

Fisher Scientific fetal bovine serum fbs
Fetal Bovine Serum Fbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fetal bovine serum fbs/product/Fisher Scientific
Average 93 stars, based on 13 article reviews
Price from $9.99 to $1999.99
fetal bovine serum fbs - by Bioz Stars, 2020-07
93/100 stars

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Selection:

Article Title: High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse model
Article Snippet: .. CD8+ T cells were isolated from healthy donor peripheral blood by negative selection (Stemcell Technologies) and cultured in X-VIVO 15 medium (Lonza) with 5% fetal bovine serum (FBS) in the presence of anti-CD3/CD28 beads (Dynabeads; Fisher Scientific). .. In Vitro AA Treatment.

Isolation:

Article Title: High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse model
Article Snippet: .. CD8+ T cells were isolated from healthy donor peripheral blood by negative selection (Stemcell Technologies) and cultured in X-VIVO 15 medium (Lonza) with 5% fetal bovine serum (FBS) in the presence of anti-CD3/CD28 beads (Dynabeads; Fisher Scientific). .. In Vitro AA Treatment.

Cell Culture:

Article Title: Coagulation factor VII is regulated by androgen receptor in breast cancer
Article Snippet: .. T-47D and MFM-223 cell lines were cultured in DMEM/F12 and DMEM media, respectively supplemented with 10% fetal bovine serum (FBS), (Fisher Scientific, Waltham, MA, USA). .. Cell culture treatment with 5α-Androstan-17β-ol-3-one (Dihydrotestosterone (DHT)), (Fisher Scientific) was carried out at 100 nM concentration to obtain an optimal AR response as described before [ , , – ].

Article Title: High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse model
Article Snippet: .. CD8+ T cells were isolated from healthy donor peripheral blood by negative selection (Stemcell Technologies) and cultured in X-VIVO 15 medium (Lonza) with 5% fetal bovine serum (FBS) in the presence of anti-CD3/CD28 beads (Dynabeads; Fisher Scientific). .. In Vitro AA Treatment.

other:

Article Title: Occurrence and Comparative Toxicity of Haloacetaldehyde Disinfection Byproducts in Drinking Water
Article Snippet: Media and fetal bovine serum (FBS) were purchased from Fisher Scientific (Itasca, IL).

Labeling:

Article Title: Reversal of drug resistance by planetary ball milled (PBM) nanoparticle loaded with resveratrol and docetaxel in prostate cancer
Article Snippet: .. The docetaxel resistant cells were labeled as PC3-R and were maintained in RPMI-1640 media supplemented with 10% fetal bovine serum (FBS), L-glutamine, nonessential amino acid, HEPES, 2mM L-glutamine and penicillin/streptomycin antibiotic solution (Fisher Scientific, Pittsburgh, PA). .. Resveratrol (RES) was purchased from Fisher Scientific (Pittsburgh, PA) and docetaxel (DTX) from Sigma (St. Louis, MO).

Modification:

Article Title: 4-Hydroxy-7-oxo-5-heptenoic Acid (HOHA) Lactone Induces Angiogenesis through Several Different Molecular Pathways
Article Snippet: .. Dulbecco’s modified Eagle’s medium (DMEM)/F12, Dulbecco’s phosphate buffered saline (DPBS), Hank’s balanced salt solution (HBSS), fetal bovine serum (FBS) and 2′,7′-dichlorodihydrofluorescein diacetate (DCFHDA) were purchased from Fisher Scientific (Pittsburgh, PA). .. A human VEGF-A ELISA kit was purchased from Piercenet (ThermoFisher Scientific, Rockford, IL).

Article Title: 6-Bromoindirubin-3′oxime (BIO) decreases proliferation and migration of canine melanoma cell lines
Article Snippet: .. Canine melanoma cell lines UCDK9M2, UCDK9M3 (gifts from Dr. M. Kent of the University of California, Davis, USA), and CML-10C2 (gift from Dr. L. Wolfe of Auburn University, USA) were maintained in modified Eagle’s medium-alpha (MEM-α) and supplemented with 10% fetal bovine serum (FBS), sodium pyruvate, L-glutamine, MEM vitamins, non-essential amino acids (all products from Fisher Scientific) at 37 °C in a humidified incubator with 5% CO2 . .. UCDK9M2 was generated from lymph node metastasis of a canine oral malignant melanoma; UCDK9M3 was generated from a canine primary oral malignant melanoma, and CML-10C2 from a canine cutaneous malignant melanoma. (2′Z,3′E)-6-Bromoindirubin-3′-oxime (BIO, Sigma-Aldrich) was dissolved in dimethyl sulfoxide (DMSO, Fisher Scientific) to a concentration of 10 mM and stored at −20 °C.

Article Title: Tet38 of Staphylococcus aureus Binds to Host Cell Receptor Complex CD36–Toll-Like Receptor 2 and Protects from Teichoic Acid Synthesis Inhibitors Tunicamycin and Congo Red
Article Snippet: .. Human lung adenocarcinoma A549 cells were purchased from the ATCC (CCL-185) and were cultivated in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) and 4 mM l -glutamine (Fisher Scientific, Waltham, MA). ..

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  • 92
    Fisher Scientific cryopreservation medium
    Immunophenotypes of P3 equine adipose-derived multipotent stromal cells (ASCs) and bone marrow-derived multipotent stromal cells (BMSCs) after culture expansion <t>post-cryopreservation.</t> The black lines represent labeled cells and the green lines represent autofluorescence
    Cryopreservation Medium, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cryopreservation medium/product/Fisher Scientific
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryopreservation medium - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Fisher Scientific msc qualified fetal bovine serum
    Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing <t>CS-FBS</t> for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering <t>MSC</t> membrane cholesterol level without compromising cell viability. * p
    Msc Qualified Fetal Bovine Serum, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msc qualified fetal bovine serum/product/Fisher Scientific
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    msc qualified fetal bovine serum - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Fisher Scientific dialyzed fbs
    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s <t>F12</t> containing dialyzed <t>FBS</t> with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting
    Dialyzed Fbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dialyzed fbs/product/Fisher Scientific
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    dialyzed fbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Immunophenotypes of P3 equine adipose-derived multipotent stromal cells (ASCs) and bone marrow-derived multipotent stromal cells (BMSCs) after culture expansion post-cryopreservation. The black lines represent labeled cells and the green lines represent autofluorescence

    Journal: Stem Cell Research & Therapy

    Article Title: Polymer-mineral scaffold augments in vivo equine multipotent stromal cell osteogenesis

    doi: 10.1186/s13287-018-0790-8

    Figure Lengend Snippet: Immunophenotypes of P3 equine adipose-derived multipotent stromal cells (ASCs) and bone marrow-derived multipotent stromal cells (BMSCs) after culture expansion post-cryopreservation. The black lines represent labeled cells and the green lines represent autofluorescence

    Article Snippet: At 70% confluence, cells were detached with 0.05% trypsin (Hyclone), suspended in cryopreservation medium (80% FBS, 10% DMEM/F-12, 10% dimethyl sulfoxide (DMSO; Fisher Scientific, Fair Lawn, NJ, USA)) at 1–1.5 × 106 cells/ml and aliquoted into 1.5-ml cryovials (Fisher Scientific).

    Techniques: Derivative Assay, Labeling

    Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p

    Journal: Stem Cell Research & Therapy

    Article Title: Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells

    doi: 10.1186/s13287-018-0830-4

    Figure Lengend Snippet: Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p

    Article Snippet: Cells flushed from the bone marrow were pelleted and resuspended in isolation medium (ISM) (α-minimum essential medium (MEM) + 1× antibiotic-antimycotic + 10% MSC qualified fetal bovine serum (FBS; all Gibco/Thermo Fisher Scientific, Waltham, MA, USA) + 1 ng/ml fibroblast growth factor (FGF)2 (R & D Systems, Minneapolis, MN)), seeded into T150 flasks (Corning Inc., Corning, NY, USA) and incubated for 3–4 days at 37 °C under 5% CO2 , then washed twice with phosphate-buffered saline (PBS; pH 7.4) and incubated in fresh isolated medium.

    Techniques: Incubation, MTS Assay

    GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s F12 containing dialyzed FBS with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting

    Journal:

    Article Title: Smallpox inhibitor of complement enzymes (SPICE): Regulation of complement activation on cells and mechanism of its cellular attachment

    doi:

    Figure Lengend Snippet: GAG sulfation is critical for SPICE binding to CHO cells. CHO cells were incubated in sulfate-free Ham’s F12 containing dialyzed FBS with 25 mM chlorate, 25 mM chlorate plus 25 mM sulfate, or normal Ham’s F12 (media control). After harvesting

    Article Snippet: CHO cells were cultured in sulfate-free Ham’s F12 medium (Washington University Tissue Culture Support Center) supplemented with 10% dialyzed FBS (Fisher Scientific) containing different concentrations (1–25 mM) of sodium chlorate.

    Techniques: Binding Assay, Incubation

    Notch1 deficiency reduces the CTGF-induced collagen gel stiffness in SMCs. (A-H) Photographs depict contractility of collagen gel embedded with VSMCs isolated from Apoe -/- and Notch1 +/- ;Apoe -/- mice. VSMCs cultured in a 6-well plate and treated with or without CTGF (10 ng/ml) for 72 hours and resuspended in a collagen solution at a concentration of 6 x 10 5 cells/ml for 48 hours. After imaging, the gels were gently released from attachment and the media was replaced with 10% FBS DMEM to induce contraction and incubated for 2 hours. The gel was imaged to determine the amount of gel contraction. Each group is represented by 6 replicates in each experiment, and repeated in 3 independent experiments. The images were analyzed using ImageJ software to measure the gel area at each time-point, from which the percentage decrease of gel area was determined. The yellow dotted lines represent the area of the collagen gel. Bar graphs represent percent decrease of gel area in different treatment groups. (J-Q) Trichrome staining of the aortic rings from Apoe -/- mice transfected with NICD, siRNA for Notch1 or Notch3 in the absence (J-M) or presence of CTGF (N-Q) . The aortic rings were transfected with NICD, non-specific siRNA (4390846, Invitrogen), siRNA Notch1 or siRNA Notch3 for 24 hours. The aortic rings were then treated using recombinant human CTGF protein for additional 48 hours. The rings were then collected and processed for histology. The red dotted lines represent the adventitial layer enriched with collagen contents. Scale bars = 50 μm . ***P

    Journal: PLoS ONE

    Article Title: Smooth muscle cell-specific Notch1 haploinsufficiency restricts the progression of abdominal aortic aneurysm by modulating CTGF expression

    doi: 10.1371/journal.pone.0178538

    Figure Lengend Snippet: Notch1 deficiency reduces the CTGF-induced collagen gel stiffness in SMCs. (A-H) Photographs depict contractility of collagen gel embedded with VSMCs isolated from Apoe -/- and Notch1 +/- ;Apoe -/- mice. VSMCs cultured in a 6-well plate and treated with or without CTGF (10 ng/ml) for 72 hours and resuspended in a collagen solution at a concentration of 6 x 10 5 cells/ml for 48 hours. After imaging, the gels were gently released from attachment and the media was replaced with 10% FBS DMEM to induce contraction and incubated for 2 hours. The gel was imaged to determine the amount of gel contraction. Each group is represented by 6 replicates in each experiment, and repeated in 3 independent experiments. The images were analyzed using ImageJ software to measure the gel area at each time-point, from which the percentage decrease of gel area was determined. The yellow dotted lines represent the area of the collagen gel. Bar graphs represent percent decrease of gel area in different treatment groups. (J-Q) Trichrome staining of the aortic rings from Apoe -/- mice transfected with NICD, siRNA for Notch1 or Notch3 in the absence (J-M) or presence of CTGF (N-Q) . The aortic rings were transfected with NICD, non-specific siRNA (4390846, Invitrogen), siRNA Notch1 or siRNA Notch3 for 24 hours. The aortic rings were then treated using recombinant human CTGF protein for additional 48 hours. The rings were then collected and processed for histology. The red dotted lines represent the adventitial layer enriched with collagen contents. Scale bars = 50 μm . ***P

    Article Snippet: The rings were plated onto chamber slides in DMEM High Glucose media (Hyclone) with 10% FBS (Fisher Scientific).

    Techniques: Isolation, Mouse Assay, Cell Culture, Concentration Assay, Imaging, Incubation, Software, Staining, Transfection, Recombinant