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Eurobio fetal bovine serum fbs
Fetal Bovine Serum Fbs, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fetal bovine serum fbs/product/Eurobio
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fetal bovine serum fbs - by Bioz Stars, 2020-07
92/100 stars

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Modification:

Article Title: Novel role for polycystin-1 in modulating cell proliferation through calcium oscillations in kidney cells
Article Snippet: .. Reagents Dulbeccos's modified Eagle's medium/F12 and minimum essential medium media, G418 antibiotic, bovine serum albumin (BSA), 1-(beta-[3-(4-methoxyphenyl) propoxy]-4-methoxyphenethyl)-1H-imidazole hydrochloride (SKF96365), cyclosporin A, gadolinium and anti-FLAG M2 mouse monoclonal antibody were obtained from Sigma-Aldrich (Milano, Italy), foetal bovine serum (FBS) was obtained from Eurobio (Celbio, Milan, Italy) and selective cell-permeable inhibitors of protein kinase C (PKC)-α and PKC-β1 (Ro-320432 and hispidin, respectively), were purchased from Calbiochem (La Jolla, CA, USA) and protease inhibitors were from Roche Diagnostics (Monza, Italy). .. Rabbit polyclonal antitubulin and antirabbit rhodamine- and FITC-conjugated antibody were obtained from Santa Cruz (DBA Italia Srl, Segrate, Italy), while rabbit polyclonal antip27 antibody was obtained from Cell Signalling Technology (Celbio SRL, Italy).

Article Title: STING-dependent paracriny shapes apoptotic priming of breast tumors in response to anti-mitotic treatment
Article Snippet: .. Cell lines and reagents Cells lines were purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Saint Aubin, France) supplemented with 2 mM glutamine and 1% penicillin/streptomycin (Gibco) and 5% Fetal Bovine Serum (FBS) (Eurobio, Court aboeuf, France). .. Lamin B2 (LMNB2 ) overexpressing cell lines were established by viral infection with retroviruses containing vector coding for LMNB2 (pQCXIB-mCherry-LMNB2) kindly offered by Lewis C. Cantley’s laboratory .

Multiple Displacement Amplification:

Article Title: Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines
Article Snippet: .. Cells MDA-MB-231 cells were grown in RPMI 1640 medium with 10% fetal bovine serum (FBS) (Eurobio), 2 mM L-glutamine. ..

Cell Culture:

Article Title: Cytotoxicity and oxidative stress induced by different metallic nanoparticles on human kidney cells
Article Snippet: .. I. Dubus (University of Rouen, France), were cultured in RPMI 1640 medium containing penicillin (100 U/ml), streptomycin (100 μg/ml) and amphotericin B (0.25 μg/ml), 2 mM L-glutamine, sodium pyruvate, non-essential aminoacids and 10 mM Hepes supplemented with 10% inactivated fetal bovine serum (FBS) (Eurobio, Les Ullis, France). .. Human Kidney (HK-2) epithelial cells were purchased from the American Type Culture Collection (ATCC, CRL-2190).

Article Title: STING-dependent paracriny shapes apoptotic priming of breast tumors in response to anti-mitotic treatment
Article Snippet: .. Cell lines and reagents Cells lines were purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Saint Aubin, France) supplemented with 2 mM glutamine and 1% penicillin/streptomycin (Gibco) and 5% Fetal Bovine Serum (FBS) (Eurobio, Court aboeuf, France). .. Lamin B2 (LMNB2 ) overexpressing cell lines were established by viral infection with retroviruses containing vector coding for LMNB2 (pQCXIB-mCherry-LMNB2) kindly offered by Lewis C. Cantley’s laboratory .

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    Eurobio 22rv1 fetal bovine serum
    ( A ). Effects of CaSR inhibition on the expression of NE markers in PC3 and <t>22RV1.</t> Cells were transfected with a siRNA control (siCTRL) or directed against CaSR (siCaSR) for 48 h (qPCR analysis were performed 48 h post-transfection). Results are normalized to control condition and are expressed as mean ± S.E.M. The statistical differences are indicated: *** p
    22rv1 Fetal Bovine Serum, supplied by Eurobio, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/22rv1 fetal bovine serum/product/Eurobio
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    22rv1 fetal bovine serum - by Bioz Stars, 2020-07
    84/100 stars
      Buy from Supplier

    92
    Eurobio foetal bovine serum fbs
    Exogenous expression of PC1 reduces Ca 2+ oscillation frequency in HEK293 cells . (a) Representative Ca 2+ oscillation patterns from individual HEK293 pSsiPKD1 (red) and HEK293 pSUPER (blue) cells before (upper traces) and after (lower traces) transient transfection with the mouse PC1 expressing plasmid (mPC1). Cells were grown on coverslips, transfected with the plasmid DNA as described in the Materials and Methods section, loaded with Fura-2-AM after 48 h and stimulated with 1% <t>FBS,</t> as described in Fig. 2 . Inset: the expression of the mouse PC1 was confirmed by Western blotting and immunofluorescence analysis, as shown in HEK293 pSUPER (pS) and HEK293 pSsiPKD1 (pSsiPKD1) transfected cells, the latter showing that mPC1 is not silenced by human PKD1 siRNA. Cells were lysed and total extracts were analysed by immunoblotting with the <t>anti-FLAG</t> M2 mouse monoclonal antibody recognizing the FLAG-tagged mouse PC1, as described in the Materials and Methods section. The antibody identified a band of about 400 kDa in only mPKD1 cDNA-transfected HEK293 pSUPER and HEK293 pSsiPKD1 cells. The ~200 kDa band was deemed to be aspecific as present in all samples. For immunofluorescence analysis, cells were fixed and treated with M2 antibody, as described in the Materials and Methods section. Upper and lower panels: contrast phase and fluorescence images. Staining of plasma membranes was indicated by arrows, mainly at cell–cell interactions. (b) Average percent reduction in Ca 2+ oscillation frequency in a 15-min period obtained after transient transfection with the mouse PC1 expressing plasmid of HEK293 pSsiPKD1 clone b 3 (red bars; n = 122 cells, five experiments) and HEK293 pSUPER cells (blue bars, n = 60, three experiments). (c) Reduction in cell proliferation in HEK293 cells stably expressing the full length mouse PC1. pCDNA3 stably transfected control cells (white bar) and cells stably transfected with the full length mouse PKD1 cDNA plasmid (grey bar) were grown for 3 days in presence of 1% FBS. Data are expressed as the average values (± SD; * P
    Foetal Bovine Serum Fbs, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/foetal bovine serum fbs/product/Eurobio
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    foetal bovine serum fbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    fbs  (Eurobio)
    92
    Eurobio fbs
    Exogenous expression of PC1 reduces Ca 2+ oscillation frequency in HEK293 cells . (a) Representative Ca 2+ oscillation patterns from individual HEK293 pSsiPKD1 (red) and HEK293 pSUPER (blue) cells before (upper traces) and after (lower traces) transient transfection with the mouse PC1 expressing plasmid (mPC1). Cells were grown on coverslips, transfected with the plasmid DNA as described in the Materials and Methods section, loaded with Fura-2-AM after 48 h and stimulated with 1% <t>FBS,</t> as described in Fig. 2 . Inset: the expression of the mouse PC1 was confirmed by Western blotting and immunofluorescence analysis, as shown in HEK293 pSUPER (pS) and HEK293 pSsiPKD1 (pSsiPKD1) transfected cells, the latter showing that mPC1 is not silenced by human PKD1 siRNA. Cells were lysed and total extracts were analysed by immunoblotting with the <t>anti-FLAG</t> M2 mouse monoclonal antibody recognizing the FLAG-tagged mouse PC1, as described in the Materials and Methods section. The antibody identified a band of about 400 kDa in only mPKD1 cDNA-transfected HEK293 pSUPER and HEK293 pSsiPKD1 cells. The ~200 kDa band was deemed to be aspecific as present in all samples. For immunofluorescence analysis, cells were fixed and treated with M2 antibody, as described in the Materials and Methods section. Upper and lower panels: contrast phase and fluorescence images. Staining of plasma membranes was indicated by arrows, mainly at cell–cell interactions. (b) Average percent reduction in Ca 2+ oscillation frequency in a 15-min period obtained after transient transfection with the mouse PC1 expressing plasmid of HEK293 pSsiPKD1 clone b 3 (red bars; n = 122 cells, five experiments) and HEK293 pSUPER cells (blue bars, n = 60, three experiments). (c) Reduction in cell proliferation in HEK293 cells stably expressing the full length mouse PC1. pCDNA3 stably transfected control cells (white bar) and cells stably transfected with the full length mouse PKD1 cDNA plasmid (grey bar) were grown for 3 days in presence of 1% FBS. Data are expressed as the average values (± SD; * P
    Fbs, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs/product/Eurobio
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    fbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    ( A ). Effects of CaSR inhibition on the expression of NE markers in PC3 and 22RV1. Cells were transfected with a siRNA control (siCTRL) or directed against CaSR (siCaSR) for 48 h (qPCR analysis were performed 48 h post-transfection). Results are normalized to control condition and are expressed as mean ± S.E.M. The statistical differences are indicated: *** p

    Journal: Cancers

    Article Title: The Calcium-Sensing Receptor is A Marker and Potential Driver of Neuroendocrine Differentiation in Prostate Cancer

    doi: 10.3390/cancers12040860

    Figure Lengend Snippet: ( A ). Effects of CaSR inhibition on the expression of NE markers in PC3 and 22RV1. Cells were transfected with a siRNA control (siCTRL) or directed against CaSR (siCaSR) for 48 h (qPCR analysis were performed 48 h post-transfection). Results are normalized to control condition and are expressed as mean ± S.E.M. The statistical differences are indicated: *** p

    Article Snippet: PC3 and 22RV1 were cultured in Roswell Park Memorial Institute medium (RPMI, BE12-702F, Lonza, Levallois-Perret, France) supplemented with 5% (PC3) or 10% (22RV1) fetal bovine serum (FBS) (CVF5VF00-01, Eurobio, Les Ulis, France) and 1% penicillin-streptomycin (PS).

    Techniques: Inhibition, Expressing, Transfection, Real-time Polymerase Chain Reaction

    Neuroendocrine markers ( A – D ) in NCI-H660, PC3 and 22RV1 cell lines and Expression of CaSR ( E ). qPCR results (mean ± SEM) are expressed in 2 -ΔΔCt and normalized to NCI-H660 cell line.

    Journal: Cancers

    Article Title: The Calcium-Sensing Receptor is A Marker and Potential Driver of Neuroendocrine Differentiation in Prostate Cancer

    doi: 10.3390/cancers12040860

    Figure Lengend Snippet: Neuroendocrine markers ( A – D ) in NCI-H660, PC3 and 22RV1 cell lines and Expression of CaSR ( E ). qPCR results (mean ± SEM) are expressed in 2 -ΔΔCt and normalized to NCI-H660 cell line.

    Article Snippet: PC3 and 22RV1 were cultured in Roswell Park Memorial Institute medium (RPMI, BE12-702F, Lonza, Levallois-Perret, France) supplemented with 5% (PC3) or 10% (22RV1) fetal bovine serum (FBS) (CVF5VF00-01, Eurobio, Les Ulis, France) and 1% penicillin-streptomycin (PS).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    ( A , B ): Effects of CaSR inhibitor on the expression of NE markers in NCI-H660 and 22RV1. Cells were treated for 72 h (NCI-H660) or 24 h (22RV1) with a CaSR inhibitor, NPS-2143 at 1 µM (NCI-H660) or 300 nM (22RV1). ( C ): Effects of CaSR activator on the expression of NE markers in PC3. Cells were treated for 24 h with a CaSR activator, R-568 (10 µM) in presence of calcium (1.2 mM). qPCR results are normalized to control condition and are expressed as mean ± S.E.M. The statistical differences are indicated: * p

    Journal: Cancers

    Article Title: The Calcium-Sensing Receptor is A Marker and Potential Driver of Neuroendocrine Differentiation in Prostate Cancer

    doi: 10.3390/cancers12040860

    Figure Lengend Snippet: ( A , B ): Effects of CaSR inhibitor on the expression of NE markers in NCI-H660 and 22RV1. Cells were treated for 72 h (NCI-H660) or 24 h (22RV1) with a CaSR inhibitor, NPS-2143 at 1 µM (NCI-H660) or 300 nM (22RV1). ( C ): Effects of CaSR activator on the expression of NE markers in PC3. Cells were treated for 24 h with a CaSR activator, R-568 (10 µM) in presence of calcium (1.2 mM). qPCR results are normalized to control condition and are expressed as mean ± S.E.M. The statistical differences are indicated: * p

    Article Snippet: PC3 and 22RV1 were cultured in Roswell Park Memorial Institute medium (RPMI, BE12-702F, Lonza, Levallois-Perret, France) supplemented with 5% (PC3) or 10% (22RV1) fetal bovine serum (FBS) (CVF5VF00-01, Eurobio, Les Ulis, France) and 1% penicillin-streptomycin (PS).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Expression of CaSR and neuroendocrine markers in NCI-H660, PC3 and 22RV1 cell lines. Immunohistochemical staining on cell pellets: all markers are expressed in NCI-H660 and 22RV1 cell lines, with nevertheless a weak expression of chromogranin in 22RV1. In PC3 cells, while both CaSR and NSE are expressed, no staining was observed for chromogranin and synaptophysin.

    Journal: Cancers

    Article Title: The Calcium-Sensing Receptor is A Marker and Potential Driver of Neuroendocrine Differentiation in Prostate Cancer

    doi: 10.3390/cancers12040860

    Figure Lengend Snippet: Expression of CaSR and neuroendocrine markers in NCI-H660, PC3 and 22RV1 cell lines. Immunohistochemical staining on cell pellets: all markers are expressed in NCI-H660 and 22RV1 cell lines, with nevertheless a weak expression of chromogranin in 22RV1. In PC3 cells, while both CaSR and NSE are expressed, no staining was observed for chromogranin and synaptophysin.

    Article Snippet: PC3 and 22RV1 were cultured in Roswell Park Memorial Institute medium (RPMI, BE12-702F, Lonza, Levallois-Perret, France) supplemented with 5% (PC3) or 10% (22RV1) fetal bovine serum (FBS) (CVF5VF00-01, Eurobio, Les Ulis, France) and 1% penicillin-streptomycin (PS).

    Techniques: Expressing, Immunohistochemistry, Staining

    Exogenous expression of PC1 reduces Ca 2+ oscillation frequency in HEK293 cells . (a) Representative Ca 2+ oscillation patterns from individual HEK293 pSsiPKD1 (red) and HEK293 pSUPER (blue) cells before (upper traces) and after (lower traces) transient transfection with the mouse PC1 expressing plasmid (mPC1). Cells were grown on coverslips, transfected with the plasmid DNA as described in the Materials and Methods section, loaded with Fura-2-AM after 48 h and stimulated with 1% FBS, as described in Fig. 2 . Inset: the expression of the mouse PC1 was confirmed by Western blotting and immunofluorescence analysis, as shown in HEK293 pSUPER (pS) and HEK293 pSsiPKD1 (pSsiPKD1) transfected cells, the latter showing that mPC1 is not silenced by human PKD1 siRNA. Cells were lysed and total extracts were analysed by immunoblotting with the anti-FLAG M2 mouse monoclonal antibody recognizing the FLAG-tagged mouse PC1, as described in the Materials and Methods section. The antibody identified a band of about 400 kDa in only mPKD1 cDNA-transfected HEK293 pSUPER and HEK293 pSsiPKD1 cells. The ~200 kDa band was deemed to be aspecific as present in all samples. For immunofluorescence analysis, cells were fixed and treated with M2 antibody, as described in the Materials and Methods section. Upper and lower panels: contrast phase and fluorescence images. Staining of plasma membranes was indicated by arrows, mainly at cell–cell interactions. (b) Average percent reduction in Ca 2+ oscillation frequency in a 15-min period obtained after transient transfection with the mouse PC1 expressing plasmid of HEK293 pSsiPKD1 clone b 3 (red bars; n = 122 cells, five experiments) and HEK293 pSUPER cells (blue bars, n = 60, three experiments). (c) Reduction in cell proliferation in HEK293 cells stably expressing the full length mouse PC1. pCDNA3 stably transfected control cells (white bar) and cells stably transfected with the full length mouse PKD1 cDNA plasmid (grey bar) were grown for 3 days in presence of 1% FBS. Data are expressed as the average values (± SD; * P

    Journal: Cell Proliferation

    Article Title: Novel role for polycystin-1 in modulating cell proliferation through calcium oscillations in kidney cells

    doi: 10.1111/j.1365-2184.2008.00529.x

    Figure Lengend Snippet: Exogenous expression of PC1 reduces Ca 2+ oscillation frequency in HEK293 cells . (a) Representative Ca 2+ oscillation patterns from individual HEK293 pSsiPKD1 (red) and HEK293 pSUPER (blue) cells before (upper traces) and after (lower traces) transient transfection with the mouse PC1 expressing plasmid (mPC1). Cells were grown on coverslips, transfected with the plasmid DNA as described in the Materials and Methods section, loaded with Fura-2-AM after 48 h and stimulated with 1% FBS, as described in Fig. 2 . Inset: the expression of the mouse PC1 was confirmed by Western blotting and immunofluorescence analysis, as shown in HEK293 pSUPER (pS) and HEK293 pSsiPKD1 (pSsiPKD1) transfected cells, the latter showing that mPC1 is not silenced by human PKD1 siRNA. Cells were lysed and total extracts were analysed by immunoblotting with the anti-FLAG M2 mouse monoclonal antibody recognizing the FLAG-tagged mouse PC1, as described in the Materials and Methods section. The antibody identified a band of about 400 kDa in only mPKD1 cDNA-transfected HEK293 pSUPER and HEK293 pSsiPKD1 cells. The ~200 kDa band was deemed to be aspecific as present in all samples. For immunofluorescence analysis, cells were fixed and treated with M2 antibody, as described in the Materials and Methods section. Upper and lower panels: contrast phase and fluorescence images. Staining of plasma membranes was indicated by arrows, mainly at cell–cell interactions. (b) Average percent reduction in Ca 2+ oscillation frequency in a 15-min period obtained after transient transfection with the mouse PC1 expressing plasmid of HEK293 pSsiPKD1 clone b 3 (red bars; n = 122 cells, five experiments) and HEK293 pSUPER cells (blue bars, n = 60, three experiments). (c) Reduction in cell proliferation in HEK293 cells stably expressing the full length mouse PC1. pCDNA3 stably transfected control cells (white bar) and cells stably transfected with the full length mouse PKD1 cDNA plasmid (grey bar) were grown for 3 days in presence of 1% FBS. Data are expressed as the average values (± SD; * P

    Article Snippet: Reagents Dulbeccos's modified Eagle's medium/F12 and minimum essential medium media, G418 antibiotic, bovine serum albumin (BSA), 1-(beta-[3-(4-methoxyphenyl) propoxy]-4-methoxyphenethyl)-1H-imidazole hydrochloride (SKF96365), cyclosporin A, gadolinium and anti-FLAG M2 mouse monoclonal antibody were obtained from Sigma-Aldrich (Milano, Italy), foetal bovine serum (FBS) was obtained from Eurobio (Celbio, Milan, Italy) and selective cell-permeable inhibitors of protein kinase C (PKC)-α and PKC-β1 (Ro-320432 and hispidin, respectively), were purchased from Calbiochem (La Jolla, CA, USA) and protease inhibitors were from Roche Diagnostics (Monza, Italy).

    Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot, Immunofluorescence, Fluorescence, Staining, Stable Transfection