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EuroClone fetal bovine serum fbs
Stability of natural and modified siRNAs in 100% fetal bovine serum at <t>37°C.</t> After incubation, siRNAs were analyzed by PAGE and ethidium bromide staining. Gel images were captured by ChemiDoc XRS (Bio-Rad) and RNA bands were quantified by Image Lab software (Bio-Rad). Signal intensity values at t 0 were set at 1.
Fetal Bovine Serum Fbs, supplied by EuroClone, used in various techniques. Bioz Stars score: 94/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fetal bovine serum fbs/product/EuroClone
Average 94 stars, based on 110 article reviews
Price from $9.99 to $1999.99
fetal bovine serum fbs - by Bioz Stars, 2020-07
94/100 stars

Images

1) Product Images from "Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages"

Article Title: Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages

Journal: BioMed Research International

doi: 10.1155/2014/901617

Stability of natural and modified siRNAs in 100% fetal bovine serum at 37°C. After incubation, siRNAs were analyzed by PAGE and ethidium bromide staining. Gel images were captured by ChemiDoc XRS (Bio-Rad) and RNA bands were quantified by Image Lab software (Bio-Rad). Signal intensity values at t 0 were set at 1.
Figure Legend Snippet: Stability of natural and modified siRNAs in 100% fetal bovine serum at 37°C. After incubation, siRNAs were analyzed by PAGE and ethidium bromide staining. Gel images were captured by ChemiDoc XRS (Bio-Rad) and RNA bands were quantified by Image Lab software (Bio-Rad). Signal intensity values at t 0 were set at 1.

Techniques Used: Modification, Incubation, Polyacrylamide Gel Electrophoresis, Staining, Software

2) Product Images from "NOS2 expression in glioma cell lines and glioma primary cell cultures: correlation with neurosphere generation and SOX-2 expression"

Article Title: NOS2 expression in glioma cell lines and glioma primary cell cultures: correlation with neurosphere generation and SOX-2 expression

Journal: Oncotarget

doi: 10.18632/oncotarget.16106

NOS2 expression and activity in primary cell cultures NOS2 mRNA expression was detected by RT-PCR in glioma primary cultures obtained from human glioma specimens at several grade of malignancy (VI, III and Low Grade (LG)), cultured in standard medium DMEM 10% FBS (St-M) and in specific medium for neurosphere generation (GSC-M). (A) GSC+: histograms showing the densitometric analysis of NOS2 expression in primary cultures kept in St-M (white bars) and in GSC-M (black bars). (B) GSC-: histograms showing the densitometric analysis of NOS2 expression in primary cultures maintained in St-M (grey bars) unable to generate neurosphere. Data are presented as mean values ± SEM of three independent experiments in duplicate. (C) RT-PCR of NOS2 from 5 GSC+ cultures. (D) RT-PCR of NOS2 from 5 GSC- cultures. Images from one representative out of three independent experiments of NOS2 expression in glioma primary cultures maintained in St-M and in GSC-M are shown. Standard = DNA ladder (100 bp), C + = Positive control (human non-small cell lung A549 cells treated with inflammatory cytokines and LPS). β-actin was used as the internal control to normalize the expression level of NOS2. (E) Nitrite concentration (μM) in primary culture #1 kept in St-M and GSC-M with or without NOS2 inhibitor, 1400W (100 μM), for 24 hrs. C+ = Positive control (human non-small cell lung cancer A549 cells treated with inflammatory cytokines and LPS for 24 hrs). Data are from one representative experiment performed in duplicate ± SD.
Figure Legend Snippet: NOS2 expression and activity in primary cell cultures NOS2 mRNA expression was detected by RT-PCR in glioma primary cultures obtained from human glioma specimens at several grade of malignancy (VI, III and Low Grade (LG)), cultured in standard medium DMEM 10% FBS (St-M) and in specific medium for neurosphere generation (GSC-M). (A) GSC+: histograms showing the densitometric analysis of NOS2 expression in primary cultures kept in St-M (white bars) and in GSC-M (black bars). (B) GSC-: histograms showing the densitometric analysis of NOS2 expression in primary cultures maintained in St-M (grey bars) unable to generate neurosphere. Data are presented as mean values ± SEM of three independent experiments in duplicate. (C) RT-PCR of NOS2 from 5 GSC+ cultures. (D) RT-PCR of NOS2 from 5 GSC- cultures. Images from one representative out of three independent experiments of NOS2 expression in glioma primary cultures maintained in St-M and in GSC-M are shown. Standard = DNA ladder (100 bp), C + = Positive control (human non-small cell lung A549 cells treated with inflammatory cytokines and LPS). β-actin was used as the internal control to normalize the expression level of NOS2. (E) Nitrite concentration (μM) in primary culture #1 kept in St-M and GSC-M with or without NOS2 inhibitor, 1400W (100 μM), for 24 hrs. C+ = Positive control (human non-small cell lung cancer A549 cells treated with inflammatory cytokines and LPS for 24 hrs). Data are from one representative experiment performed in duplicate ± SD.

Techniques Used: Expressing, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Positive Control, Concentration Assay

3) Product Images from "Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages"

Article Title: Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages

Journal: BioMed Research International

doi: 10.1155/2014/901617

Stability of natural and modified siRNAs in 100% fetal bovine serum at 37°C. After incubation, siRNAs were analyzed by PAGE and ethidium bromide staining. Gel images were captured by ChemiDoc XRS (Bio-Rad) and RNA bands were quantified by Image Lab software (Bio-Rad). Signal intensity values at t 0 were set at 1.
Figure Legend Snippet: Stability of natural and modified siRNAs in 100% fetal bovine serum at 37°C. After incubation, siRNAs were analyzed by PAGE and ethidium bromide staining. Gel images were captured by ChemiDoc XRS (Bio-Rad) and RNA bands were quantified by Image Lab software (Bio-Rad). Signal intensity values at t 0 were set at 1.

Techniques Used: Modification, Incubation, Polyacrylamide Gel Electrophoresis, Staining, Software

4) Product Images from "Alternatively Activated (M2) Macrophage Phenotype Is Inducible by Endothelin-1 in Cultured Human Macrophages"

Article Title: Alternatively Activated (M2) Macrophage Phenotype Is Inducible by Endothelin-1 in Cultured Human Macrophages

Journal: PLoS ONE

doi: 10.1371/journal.pone.0166433

Evaluation of CD68, CD204 and CD206 expression in cultured THP-1-derived macrophages. Immunocytochemistry of CD68 (marker of macrophage activation), CD204 and CD206 protein expression in cultured THP-1-derived macrophages (M0 macrophages) treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). Immunocytochemistry was performed on four independent in vitro experiments. The detection of protein expression was performed evaluating the same number of cells by light microscopy (magnification 40X).
Figure Legend Snippet: Evaluation of CD68, CD204 and CD206 expression in cultured THP-1-derived macrophages. Immunocytochemistry of CD68 (marker of macrophage activation), CD204 and CD206 protein expression in cultured THP-1-derived macrophages (M0 macrophages) treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). Immunocytochemistry was performed on four independent in vitro experiments. The detection of protein expression was performed evaluating the same number of cells by light microscopy (magnification 40X).

Techniques Used: Expressing, Cell Culture, Derivative Assay, Immunocytochemistry, Marker, Activation Assay, In Vitro, Light Microscopy

Evaluation of gene expression of M2 macrophage phenotype markers and TGFbeta1 in cultured human monocyte-derived macrophages. Quantitative real time polymerase chain reaction (qRT-PCR) of CD204, CD206, CD163, IL-10, CCL-22 and TGFbeta1 gene expression in cultured human monocyte-derived macrophages treated for 6 days with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured human monocyte-derived macrophages maintained for 6 days in RPMI at 10% of FBS were used as untreated cells. The qRT-PCR was performed on six independent in vitro experiments and the data of CD204, CD206, CD163, IL-10, CCL-22 and TGFbeta1 gene expression are shown as mean±SD and indicated as increase in gene expression.
Figure Legend Snippet: Evaluation of gene expression of M2 macrophage phenotype markers and TGFbeta1 in cultured human monocyte-derived macrophages. Quantitative real time polymerase chain reaction (qRT-PCR) of CD204, CD206, CD163, IL-10, CCL-22 and TGFbeta1 gene expression in cultured human monocyte-derived macrophages treated for 6 days with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured human monocyte-derived macrophages maintained for 6 days in RPMI at 10% of FBS were used as untreated cells. The qRT-PCR was performed on six independent in vitro experiments and the data of CD204, CD206, CD163, IL-10, CCL-22 and TGFbeta1 gene expression are shown as mean±SD and indicated as increase in gene expression.

Techniques Used: Expressing, Cell Culture, Derivative Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, In Vitro

Evaluation of pro-fibrotic MMP-9 production in cultured macrophages. Evaluation by gel zymography of MMP-9 production and related densitometric analysis in cultured M0-macrophages and human monocyte-derived macrophages. (A) Cultured M0 macrophages were treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before the stimulation with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). (B) Cultured human monocyte-derived macrophages treated for 6 days with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (10 -5 M) for 1 hour before the stimulation with ET-1. Cultured human monocyte-derived macrophages maintained for 6 days in RPMI at 10% of FBS were used as untreated cells. Gel zymography was performed on six independent in vitro experiments and the data of MMP-9 production are shown as mean±SD.
Figure Legend Snippet: Evaluation of pro-fibrotic MMP-9 production in cultured macrophages. Evaluation by gel zymography of MMP-9 production and related densitometric analysis in cultured M0-macrophages and human monocyte-derived macrophages. (A) Cultured M0 macrophages were treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before the stimulation with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). (B) Cultured human monocyte-derived macrophages treated for 6 days with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (10 -5 M) for 1 hour before the stimulation with ET-1. Cultured human monocyte-derived macrophages maintained for 6 days in RPMI at 10% of FBS were used as untreated cells. Gel zymography was performed on six independent in vitro experiments and the data of MMP-9 production are shown as mean±SD.

Techniques Used: Cell Culture, Zymography, Derivative Assay, In Vitro

Evaluation of protein synthesis of M2 phenotype markers and TGFbeta1 in cultured human monocyte-derived macrophages. Western blotting and related densitometric analysis of CD204, CD206, CD163 and TGFbeta1 protein synthesis in cultured human monocyte-derived macrophages treated for 6 days with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured human monocyte-derived macrophages maintained for 6 days in RPMI at 10% of FBS were used as untreated cells. Western blotting was performed on six independent in vitro experiments. The data of CD204, CD206, CD163 and TGFbeta1 protein synthesis are shown as mean±SD and indicated as increase in protein synthesis.
Figure Legend Snippet: Evaluation of protein synthesis of M2 phenotype markers and TGFbeta1 in cultured human monocyte-derived macrophages. Western blotting and related densitometric analysis of CD204, CD206, CD163 and TGFbeta1 protein synthesis in cultured human monocyte-derived macrophages treated for 6 days with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured human monocyte-derived macrophages maintained for 6 days in RPMI at 10% of FBS were used as untreated cells. Western blotting was performed on six independent in vitro experiments. The data of CD204, CD206, CD163 and TGFbeta1 protein synthesis are shown as mean±SD and indicated as increase in protein synthesis.

Techniques Used: Cell Culture, Derivative Assay, Western Blot, In Vitro

Evaluation of CD68, CD204 and CD206 expression in cultured human monocyte-derived macrophages. Immunocytochemistry of CD68 (marker of macrophage activation), CD204 and CD206 protein expression in cultured human monocyte-derived macrophages treated for 6 days with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured human monocyte-derived macrophages maintained for 6 days in RPMI at 10% of FBS were used as untreated cells. Immunocytochemistry was performed on four independent in vitro experiments. The detection of protein expression was performed evaluating the same number of cells by light microscopy (magnification 40X).
Figure Legend Snippet: Evaluation of CD68, CD204 and CD206 expression in cultured human monocyte-derived macrophages. Immunocytochemistry of CD68 (marker of macrophage activation), CD204 and CD206 protein expression in cultured human monocyte-derived macrophages treated for 6 days with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured human monocyte-derived macrophages maintained for 6 days in RPMI at 10% of FBS were used as untreated cells. Immunocytochemistry was performed on four independent in vitro experiments. The detection of protein expression was performed evaluating the same number of cells by light microscopy (magnification 40X).

Techniques Used: Expressing, Cell Culture, Derivative Assay, Immunocytochemistry, Marker, Activation Assay, In Vitro, Light Microscopy

Evaluation of protein synthesis of M2 phenotype markers in cultured THP-1-derived macrophages. Western blotting and related densitometric analysis of CD204, CD206 and CD163 protein synthesis in cultured THP-1-derived macrophages (M0 macrophages) treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). Western blotting was performed on six independent in vitro experiments. The data of CD204, CD206 and CD163 protein synthesis are shown as mean±SD and indicated as increase in protein synthesis.
Figure Legend Snippet: Evaluation of protein synthesis of M2 phenotype markers in cultured THP-1-derived macrophages. Western blotting and related densitometric analysis of CD204, CD206 and CD163 protein synthesis in cultured THP-1-derived macrophages (M0 macrophages) treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). Western blotting was performed on six independent in vitro experiments. The data of CD204, CD206 and CD163 protein synthesis are shown as mean±SD and indicated as increase in protein synthesis.

Techniques Used: Cell Culture, Derivative Assay, Western Blot, In Vitro

Evaluation of gene expression of M2 macrophage phenotype markers in cultured THP-1-derived macrophages. Quantitative real time polymerase chain reaction (qRT-PCR) of CD204, CD206, CD163, IL-10 and CCL-22 gene expression in cultured THP-1-derived macrophages (M0 macrophages) treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). The qRT-PCR was performed on six independent in vitro experiments and the data of CD204, CD206, CD163, IL-10 and CCL-22 gene expression are shown as mean±SD and indicated as increase in gene expression.
Figure Legend Snippet: Evaluation of gene expression of M2 macrophage phenotype markers in cultured THP-1-derived macrophages. Quantitative real time polymerase chain reaction (qRT-PCR) of CD204, CD206, CD163, IL-10 and CCL-22 gene expression in cultured THP-1-derived macrophages (M0 macrophages) treated for 72 hours with ET-1 (100nM) and IL-4 (10ng/mL) alone, or pre-treated with ET A/B RA (bosentan, 10 -5 M) for 1 hour before being stimulated with ET-1. Cultured M0 macrophages maintained for 72 hours in RPMI at 5% of FBS were used as controls (M0-controls). The qRT-PCR was performed on six independent in vitro experiments and the data of CD204, CD206, CD163, IL-10 and CCL-22 gene expression are shown as mean±SD and indicated as increase in gene expression.

Techniques Used: Expressing, Cell Culture, Derivative Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, In Vitro

5) Product Images from "Effects of Iron on Extracellular and Intracellular Growth of Penicillium marneffei"

Article Title: Effects of Iron on Extracellular and Intracellular Growth of Penicillium marneffei

Journal: Infection and Immunity

doi:

Effect of different concentrations of FBS (a) and iron (FeCl 3 ) (b) on P. marneffei growth in axenic medium. The assays were performed with MEM containing 20 mM HEPES buffer, in microtiter wells, in triplicate, and with 5 × 10 3 conidia/well for 48 h at 37°C. Fungal growth was assessed by MTT reduction. Data are expressed as the mean ± standard deviation of a representative experiment ( n = 4). OD, optical density.
Figure Legend Snippet: Effect of different concentrations of FBS (a) and iron (FeCl 3 ) (b) on P. marneffei growth in axenic medium. The assays were performed with MEM containing 20 mM HEPES buffer, in microtiter wells, in triplicate, and with 5 × 10 3 conidia/well for 48 h at 37°C. Fungal growth was assessed by MTT reduction. Data are expressed as the mean ± standard deviation of a representative experiment ( n = 4). OD, optical density.

Techniques Used: MTT Assay, Standard Deviation

Related Articles

Transfection:

Article Title: Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages
Article Snippet: .. Cell Cultures, Transfections, and Luciferase Assay HeLa cells were grown at 37°C, 5% CO2 in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS) (EuroClone), 100 units mL−1 penicillin, and 100 mg mL−1 streptomycin (EuroClone). ..

Luciferase:

Article Title: Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages
Article Snippet: .. Cell Cultures, Transfections, and Luciferase Assay HeLa cells were grown at 37°C, 5% CO2 in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS) (EuroClone), 100 units mL−1 penicillin, and 100 mg mL−1 streptomycin (EuroClone). ..

Multiple Displacement Amplification:

Article Title: Active Fraction from Embryo Fish Extracts Induces Reversion of the Malignant Invasive Phenotype in Breast Cancer through Down-Regulation of TCTP and Modulation of E-cadherin/β-catenin Pathway
Article Snippet: .. MCF-7 and MDA-MB-231 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics (penicillin 100 IU/mL, streptomycin 100 µg/mL, gentamycin 200 µg/mL; all from Euroclone Ltd., Cramlington, UK), MCF-10A were grown in Dulbecco’s modified Eagle’s medium/ nutrient mixture F12 Ham (Sigma-Aldrich, Merck, Darmstadt, Germany) supplemented with 10% horse serum (Euroclone Ltd., Cramlington, UK) and EGF 500 µ/5 mL (Santa Cruz Biotechnologies, Dallas, TX, USA), Hydrocortisone (50 µM), cholera toxin (0.5 mg/mL), insulin (10 mg/mL) (all from Sigma Chemical Co) and antibiotics (penicillin 100 IU/mL, streptomycin 100 µg/mL, gentamycin 200 µg/mL; all from Euroclone Ltd., Cramlington, UK). ..

Incubation:

Article Title: Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages
Article Snippet: .. Therefore, the nuclease resistance of 2–19 was investigated through incubation in 100% fetal bovine serum (FBS) at 37°C using unmodified siRNA 1 as control ( ). .. At various incubation times, aliquots of each siRNA were analyzed by electrophoresis on 15% polyacrylamide gels to detect any degradation products.

other:

Article Title: Carbonic anhydrase IX is a marker of hypoxia and correlates with higher Gleason scores and ISUP grading in prostate cancer
Article Snippet: Cells were grown in RPMI-1640 (EuroClone) (LNCaP) and DMEM (PC-3), supplemented with 10 % (vol/vol) fetal bovine serum (FBS) (EuroClone), L-glutamine (EuroClone) and 1 % (vol/vol) antibiotic/antimycotic solution (Gibco, Invitrogen S.r.l.).

Modification:

Article Title: Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages
Article Snippet: .. Cell Cultures, Transfections, and Luciferase Assay HeLa cells were grown at 37°C, 5% CO2 in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS) (EuroClone), 100 units mL−1 penicillin, and 100 mg mL−1 streptomycin (EuroClone). ..

Article Title: Active Fraction from Embryo Fish Extracts Induces Reversion of the Malignant Invasive Phenotype in Breast Cancer through Down-Regulation of TCTP and Modulation of E-cadherin/β-catenin Pathway
Article Snippet: .. MCF-7 and MDA-MB-231 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics (penicillin 100 IU/mL, streptomycin 100 µg/mL, gentamycin 200 µg/mL; all from Euroclone Ltd., Cramlington, UK), MCF-10A were grown in Dulbecco’s modified Eagle’s medium/ nutrient mixture F12 Ham (Sigma-Aldrich, Merck, Darmstadt, Germany) supplemented with 10% horse serum (Euroclone Ltd., Cramlington, UK) and EGF 500 µ/5 mL (Santa Cruz Biotechnologies, Dallas, TX, USA), Hydrocortisone (50 µM), cholera toxin (0.5 mg/mL), insulin (10 mg/mL) (all from Sigma Chemical Co) and antibiotics (penicillin 100 IU/mL, streptomycin 100 µg/mL, gentamycin 200 µg/mL; all from Euroclone Ltd., Cramlington, UK). ..

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    EuroClone fbs
    Clinical grade DCs display different biological features compared to standard S-DCs . (A) Morphological differences of DCs grown in <t>RPMI</t> + <t>10%FBS</t> (S-DCs) and in X-VIVO 15 (X-DCs) (a,b, respectively) visualized by phase contrast inverted microscope (ZEISS West Germany IM35, 3,2X). (B) Flow cytometry analysis of immature and mature DCs (iDCs and mDCs, respectively) grown in FBS-RPMI or X-VIVO 15 after 6 day culture in the presence of GM-CSF, IL-4. iDCs were matured with IL-6, IL-1β, PGE-2, and TNF-α on day 5. IgG 1 PE and FITC were used as isotype controls and employed to evaluate fluorescence signal background and set the gate. Results were shown as value of Mean Fluorescence Intensity (MFI) of each phenotypic marker subtracted of the corresponded negative control MFI value and depicted as scatter plot (black circle for S-DCs; black triangles for X-DCs). Statistically significant differences between S-DCs vs X-DCs were indicated (* p
    Fbs, supplied by EuroClone, used in various techniques. Bioz Stars score: 94/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs/product/EuroClone
    Average 94 stars, based on 145 article reviews
    Price from $9.99 to $1999.99
    fbs - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    EuroClone heat inactivated fbs hi fbs
    Phase-contrast inverted microscope images of silenced cells (shRNA 4E) grown in <t>DMEM/F12</t> supplemented with 10% <t>HI-FBS</t> or NH OsteoDiff medium shRNA 4E cells grown in osteogenic medium exhibited multilayer growth with cells retracting from some areas and grouping into multicellular aggregates or nodules with abundant dense deposits. The images (acquired with Hund Wetzlar, Wilovert, magnification: 200×) are representative of two independent experiments. Bar =100 µm.
    Heat Inactivated Fbs Hi Fbs, supplied by EuroClone, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat inactivated fbs hi fbs/product/EuroClone
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    heat inactivated fbs hi fbs - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Clinical grade DCs display different biological features compared to standard S-DCs . (A) Morphological differences of DCs grown in RPMI + 10%FBS (S-DCs) and in X-VIVO 15 (X-DCs) (a,b, respectively) visualized by phase contrast inverted microscope (ZEISS West Germany IM35, 3,2X). (B) Flow cytometry analysis of immature and mature DCs (iDCs and mDCs, respectively) grown in FBS-RPMI or X-VIVO 15 after 6 day culture in the presence of GM-CSF, IL-4. iDCs were matured with IL-6, IL-1β, PGE-2, and TNF-α on day 5. IgG 1 PE and FITC were used as isotype controls and employed to evaluate fluorescence signal background and set the gate. Results were shown as value of Mean Fluorescence Intensity (MFI) of each phenotypic marker subtracted of the corresponded negative control MFI value and depicted as scatter plot (black circle for S-DCs; black triangles for X-DCs). Statistically significant differences between S-DCs vs X-DCs were indicated (* p

    Journal: Frontiers in Immunology

    Article Title: Tumor-Derived Microvesicles Enhance Cross-Processing Ability of Clinical Grade Dendritic Cells

    doi: 10.3389/fimmu.2018.02481

    Figure Lengend Snippet: Clinical grade DCs display different biological features compared to standard S-DCs . (A) Morphological differences of DCs grown in RPMI + 10%FBS (S-DCs) and in X-VIVO 15 (X-DCs) (a,b, respectively) visualized by phase contrast inverted microscope (ZEISS West Germany IM35, 3,2X). (B) Flow cytometry analysis of immature and mature DCs (iDCs and mDCs, respectively) grown in FBS-RPMI or X-VIVO 15 after 6 day culture in the presence of GM-CSF, IL-4. iDCs were matured with IL-6, IL-1β, PGE-2, and TNF-α on day 5. IgG 1 PE and FITC were used as isotype controls and employed to evaluate fluorescence signal background and set the gate. Results were shown as value of Mean Fluorescence Intensity (MFI) of each phenotypic marker subtracted of the corresponded negative control MFI value and depicted as scatter plot (black circle for S-DCs; black triangles for X-DCs). Statistically significant differences between S-DCs vs X-DCs were indicated (* p

    Article Snippet: To generate MVs, cells were cultured 3.5 × 105 cells/mL in RPMI 1640 (Sigma-Aldrich) complemented with 2% FBS (Euroclone) for 48 h. Supernatant (70 mL/tube) underwent to serial centrifugation steps at 4°C (250 × g for 10 min, 550 × g for 30 min, 1,500 × g for 30 min) (Allegra™ 6R Centrifuges, Beckman Coulter, USA).

    Techniques: Inverted Microscopy, Flow Cytometry, Cytometry, Fluorescence, Marker, Negative Control

    Stability of natural and modified siRNAs in 100% fetal bovine serum at 37°C. After incubation, siRNAs were analyzed by PAGE and ethidium bromide staining. Gel images were captured by ChemiDoc XRS (Bio-Rad) and RNA bands were quantified by Image Lab software (Bio-Rad). Signal intensity values at t 0 were set at 1.

    Journal: BioMed Research International

    Article Title: Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages

    doi: 10.1155/2014/901617

    Figure Lengend Snippet: Stability of natural and modified siRNAs in 100% fetal bovine serum at 37°C. After incubation, siRNAs were analyzed by PAGE and ethidium bromide staining. Gel images were captured by ChemiDoc XRS (Bio-Rad) and RNA bands were quantified by Image Lab software (Bio-Rad). Signal intensity values at t 0 were set at 1.

    Article Snippet: Therefore, the nuclease resistance of 2–19 was investigated through incubation in 100% fetal bovine serum (FBS) at 37°C using unmodified siRNA 1 as control ( ).

    Techniques: Modification, Incubation, Polyacrylamide Gel Electrophoresis, Staining, Software

    MSC growth in the presence of fetal bovine serum (FBS) or PL/PI-PL lots. Growth of three MSC populations (MSC9, MSC180, MSC082) at passage1,2,3 (P1,P2,P3) in the presence of PL1, PI-PL11, PI-PL16, PI-PL43/44 and FBS, expressed as the absolute number of cells obtained in cultures in D-MEM medium. Cell number, expressed by a logarithmic scale, shows in any case an irrelevant effect of FBS on MSC growth when used as a supplement of a basic culture medium.

    Journal: Journal of Translational Medicine

    Article Title: Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells

    doi: 10.1186/1479-5876-12-28

    Figure Lengend Snippet: MSC growth in the presence of fetal bovine serum (FBS) or PL/PI-PL lots. Growth of three MSC populations (MSC9, MSC180, MSC082) at passage1,2,3 (P1,P2,P3) in the presence of PL1, PI-PL11, PI-PL16, PI-PL43/44 and FBS, expressed as the absolute number of cells obtained in cultures in D-MEM medium. Cell number, expressed by a logarithmic scale, shows in any case an irrelevant effect of FBS on MSC growth when used as a supplement of a basic culture medium.

    Article Snippet: Moreover, in some experiments BM-MNC were cultured in D-MEM additioned with 10% FBS (EuroClone, Milan, Italy).

    Techniques:

    Phase-contrast inverted microscope images of silenced cells (shRNA 4E) grown in DMEM/F12 supplemented with 10% HI-FBS or NH OsteoDiff medium shRNA 4E cells grown in osteogenic medium exhibited multilayer growth with cells retracting from some areas and grouping into multicellular aggregates or nodules with abundant dense deposits. The images (acquired with Hund Wetzlar, Wilovert, magnification: 200×) are representative of two independent experiments. Bar =100 µm.

    Journal: Bioscience Reports

    Article Title: Caspase-independent programmed cell death triggers Ca2PO4 deposition in an in vitro model of nephrocalcinosis

    doi: 10.1042/BSR20171228

    Figure Lengend Snippet: Phase-contrast inverted microscope images of silenced cells (shRNA 4E) grown in DMEM/F12 supplemented with 10% HI-FBS or NH OsteoDiff medium shRNA 4E cells grown in osteogenic medium exhibited multilayer growth with cells retracting from some areas and grouping into multicellular aggregates or nodules with abundant dense deposits. The images (acquired with Hund Wetzlar, Wilovert, magnification: 200×) are representative of two independent experiments. Bar =100 µm.

    Article Snippet: HK-2 cells were maintained in a mixture of Ham’s F12 and Dulbecco’s modified Eagle’s growth medium (DMEM/F12; EuroClone, CelBio) supplemented with 10% heat-inactivated FBS (HI-FBS), 2 mM l -glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (EuroClone, CelBio).

    Techniques: Inverted Microscopy, shRNA

    Calcium detection in HK-2 cells cultured in either DMEM/F12 supplemented with 10% HI-FBS or NH OsteoDiff medium ( A ) Light microscope images (Diaplan light microscope (Leitz), magnification: 100–200×) of von Kossa staining revealed calcium deposits in some cells and nodules in the Negative Control and silenced (shRNA 4E) cells grown in osteogenic medium for 15 days. No calcium deposition was seen in Negative Control or silenced cells cultured under standard conditions (DMEM/F12 supplemented with 10% HI-FBS). Low magnification images showed much more abundant dark deposits in the silenced than in the Negative Control cells. The images are representative of two independent experiments. Bars =100 µm (left) and 50 µm (right). ( B ) Quantitative analysis of von Kossa staining with morphometric analyses on days 5 and 15. Statistically significant differences emerged between the shRNA 4E cells and the WT and Negative Control cells at 5 (* P

    Journal: Bioscience Reports

    Article Title: Caspase-independent programmed cell death triggers Ca2PO4 deposition in an in vitro model of nephrocalcinosis

    doi: 10.1042/BSR20171228

    Figure Lengend Snippet: Calcium detection in HK-2 cells cultured in either DMEM/F12 supplemented with 10% HI-FBS or NH OsteoDiff medium ( A ) Light microscope images (Diaplan light microscope (Leitz), magnification: 100–200×) of von Kossa staining revealed calcium deposits in some cells and nodules in the Negative Control and silenced (shRNA 4E) cells grown in osteogenic medium for 15 days. No calcium deposition was seen in Negative Control or silenced cells cultured under standard conditions (DMEM/F12 supplemented with 10% HI-FBS). Low magnification images showed much more abundant dark deposits in the silenced than in the Negative Control cells. The images are representative of two independent experiments. Bars =100 µm (left) and 50 µm (right). ( B ) Quantitative analysis of von Kossa staining with morphometric analyses on days 5 and 15. Statistically significant differences emerged between the shRNA 4E cells and the WT and Negative Control cells at 5 (* P

    Article Snippet: HK-2 cells were maintained in a mixture of Ham’s F12 and Dulbecco’s modified Eagle’s growth medium (DMEM/F12; EuroClone, CelBio) supplemented with 10% heat-inactivated FBS (HI-FBS), 2 mM l -glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (EuroClone, CelBio).

    Techniques: Cell Culture, Light Microscopy, Staining, Negative Control, shRNA

    ESEM analysis of HK-2 cells cultured in DMEM/F12 supplemented with 10% HI-FBS or NH OsteoDiff medium ( A ) In silenced cells, ESEM images and spectra confirmed the presence of calcium (Ca) and phosphate (P) in the nodules on day 15 (green arrow). No calcium-phosphate (Ca 2 PO 4 ) deposits were seen in silenced cells cultured under standard conditions. The images are representative of two independent experiments. Bars =100 µm (left), 50 µm (right). ( B ) Quantitative analysis of calcium and phosphate levels in WT, Negative Control, and shRNA 4E cells grown in NH OsteoDiff medium for 15 days. Bar =500 µm. Statistically significant differences emerged using a non-parametric test (Mann–Whitney U test), and Primer software (McGraw-Hill) (** P

    Journal: Bioscience Reports

    Article Title: Caspase-independent programmed cell death triggers Ca2PO4 deposition in an in vitro model of nephrocalcinosis

    doi: 10.1042/BSR20171228

    Figure Lengend Snippet: ESEM analysis of HK-2 cells cultured in DMEM/F12 supplemented with 10% HI-FBS or NH OsteoDiff medium ( A ) In silenced cells, ESEM images and spectra confirmed the presence of calcium (Ca) and phosphate (P) in the nodules on day 15 (green arrow). No calcium-phosphate (Ca 2 PO 4 ) deposits were seen in silenced cells cultured under standard conditions. The images are representative of two independent experiments. Bars =100 µm (left), 50 µm (right). ( B ) Quantitative analysis of calcium and phosphate levels in WT, Negative Control, and shRNA 4E cells grown in NH OsteoDiff medium for 15 days. Bar =500 µm. Statistically significant differences emerged using a non-parametric test (Mann–Whitney U test), and Primer software (McGraw-Hill) (** P

    Article Snippet: HK-2 cells were maintained in a mixture of Ham’s F12 and Dulbecco’s modified Eagle’s growth medium (DMEM/F12; EuroClone, CelBio) supplemented with 10% heat-inactivated FBS (HI-FBS), 2 mM l -glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (EuroClone, CelBio).

    Techniques: Cell Culture, Negative Control, shRNA, MANN-WHITNEY, Software

    Cell proliferation and viability Results of Methyl Blue assay (based on optical density) of WT, Negative Control, and silenced (shRNA 4E) cell growth in standard (DMEM/F12 supplemented with 10% HI-FBS) and osteogenic (NH OsteoDiff) conditions. Data are presented as the mean ± S.D. of three independent experiments. Statistically significant differences emerged between the shRNA 4E and the WT and Negative Control cells (** P

    Journal: Bioscience Reports

    Article Title: Caspase-independent programmed cell death triggers Ca2PO4 deposition in an in vitro model of nephrocalcinosis

    doi: 10.1042/BSR20171228

    Figure Lengend Snippet: Cell proliferation and viability Results of Methyl Blue assay (based on optical density) of WT, Negative Control, and silenced (shRNA 4E) cell growth in standard (DMEM/F12 supplemented with 10% HI-FBS) and osteogenic (NH OsteoDiff) conditions. Data are presented as the mean ± S.D. of three independent experiments. Statistically significant differences emerged between the shRNA 4E and the WT and Negative Control cells (** P

    Article Snippet: HK-2 cells were maintained in a mixture of Ham’s F12 and Dulbecco’s modified Eagle’s growth medium (DMEM/F12; EuroClone, CelBio) supplemented with 10% heat-inactivated FBS (HI-FBS), 2 mM l -glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (EuroClone, CelBio).

    Techniques: Negative Control, shRNA