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Biowest SAS fetal bovine serum fbs
Crizotinib-induced apoptosis in Ba/F3 cells transformed by NPM-ALK. Ba/F3 cells were infected with an empty virus (-) and retrovirus expressing NPM-ALK or TEL-JAK2. (A) Whole cell lysates were immunoblotted with an anti-ALK antibody, anti-Flag antibody, anti-JAK2 antibody, anti-phospho-STAT3 antibody, anti-STAT3 antibody, anti-phospho-STAT5 antibody, anti-STAT5 antibody or anti-β-actin antibody. (B) Transduced Ba/F3 cells were incubated with <t>RPMI</t> containing 10% <t>FBS</t> in the absence of IL-3 for 2 days. Viable cell numbers at 24 hr and 48 hr were counted by the Trypan blue exclusion method. Values are the mean ± SD of three independent experiments. (C-F) Transduced Ba/F3 cells were incubated with RPMI containing 10% FBS in the presence of crizotinib (0.25, 0.5, 1 μM) and MMC (0.1, 1, 10 μg/mL) for 24 hr. (C) Cell viability was measured by the WST assay. Values are the mean ± S.D. of four independent experiments. (D) Cells were fixed, treated with propidium iodide, and subjected to a flow cytometric analysis. The percentages of cells in the sub-G1 phase were graphed. Values are the mean ± S.D. of four independent experiments. (E) To evaluate the apoptotic cell death, the chromatin DNA was isolated from cells and subjected to agarose gel electrophoresis. (F) Whole cell lysates were prepared and the phosphorylation of STAT3 and STAT5 was analyzed by immunoblotting. The relative phosphorylation level of STAT3 and STAT5 is shown in the graphs. Values are given as the mean ± SD of three independent experiments. *** P
Fetal Bovine Serum Fbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Alpha-tocopherol attenuates the anti-tumor activity of crizotinib against cells transformed by NPM-ALK"

Article Title: Alpha-tocopherol attenuates the anti-tumor activity of crizotinib against cells transformed by NPM-ALK

Journal: PLoS ONE

doi: 10.1371/journal.pone.0183003

Crizotinib-induced apoptosis in Ba/F3 cells transformed by NPM-ALK. Ba/F3 cells were infected with an empty virus (-) and retrovirus expressing NPM-ALK or TEL-JAK2. (A) Whole cell lysates were immunoblotted with an anti-ALK antibody, anti-Flag antibody, anti-JAK2 antibody, anti-phospho-STAT3 antibody, anti-STAT3 antibody, anti-phospho-STAT5 antibody, anti-STAT5 antibody or anti-β-actin antibody. (B) Transduced Ba/F3 cells were incubated with RPMI containing 10% FBS in the absence of IL-3 for 2 days. Viable cell numbers at 24 hr and 48 hr were counted by the Trypan blue exclusion method. Values are the mean ± SD of three independent experiments. (C-F) Transduced Ba/F3 cells were incubated with RPMI containing 10% FBS in the presence of crizotinib (0.25, 0.5, 1 μM) and MMC (0.1, 1, 10 μg/mL) for 24 hr. (C) Cell viability was measured by the WST assay. Values are the mean ± S.D. of four independent experiments. (D) Cells were fixed, treated with propidium iodide, and subjected to a flow cytometric analysis. The percentages of cells in the sub-G1 phase were graphed. Values are the mean ± S.D. of four independent experiments. (E) To evaluate the apoptotic cell death, the chromatin DNA was isolated from cells and subjected to agarose gel electrophoresis. (F) Whole cell lysates were prepared and the phosphorylation of STAT3 and STAT5 was analyzed by immunoblotting. The relative phosphorylation level of STAT3 and STAT5 is shown in the graphs. Values are given as the mean ± SD of three independent experiments. *** P
Figure Legend Snippet: Crizotinib-induced apoptosis in Ba/F3 cells transformed by NPM-ALK. Ba/F3 cells were infected with an empty virus (-) and retrovirus expressing NPM-ALK or TEL-JAK2. (A) Whole cell lysates were immunoblotted with an anti-ALK antibody, anti-Flag antibody, anti-JAK2 antibody, anti-phospho-STAT3 antibody, anti-STAT3 antibody, anti-phospho-STAT5 antibody, anti-STAT5 antibody or anti-β-actin antibody. (B) Transduced Ba/F3 cells were incubated with RPMI containing 10% FBS in the absence of IL-3 for 2 days. Viable cell numbers at 24 hr and 48 hr were counted by the Trypan blue exclusion method. Values are the mean ± SD of three independent experiments. (C-F) Transduced Ba/F3 cells were incubated with RPMI containing 10% FBS in the presence of crizotinib (0.25, 0.5, 1 μM) and MMC (0.1, 1, 10 μg/mL) for 24 hr. (C) Cell viability was measured by the WST assay. Values are the mean ± S.D. of four independent experiments. (D) Cells were fixed, treated with propidium iodide, and subjected to a flow cytometric analysis. The percentages of cells in the sub-G1 phase were graphed. Values are the mean ± S.D. of four independent experiments. (E) To evaluate the apoptotic cell death, the chromatin DNA was isolated from cells and subjected to agarose gel electrophoresis. (F) Whole cell lysates were prepared and the phosphorylation of STAT3 and STAT5 was analyzed by immunoblotting. The relative phosphorylation level of STAT3 and STAT5 is shown in the graphs. Values are given as the mean ± SD of three independent experiments. *** P

Techniques Used: Transformation Assay, Infection, Expressing, Incubation, WST Assay, Flow Cytometry, Isolation, Agarose Gel Electrophoresis

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Article Title: Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies
Article Snippet: After 14 days, hepatic maturation was induced for additional 14 days by application of 1.7% DMSO (Sigma) to the culture medium. .. Human epithelial lung carcinoma cells (A549; ATCC, Manassas, VA, USA) were cultured using Dulbecco´s Modified Eagle Medium (DMEM) high glucose (Biowest) supplemented with 10% fetal bovine serum (FBS; c.c.pro), 2 mM l -Glutamine (Biowest) and 1% penicillin/streptomycin (P/S; Biowest). .. Human extracellular matrix (hECM) was isolated from a lung of a deceased patient.

Modification:

Article Title: Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies
Article Snippet: After 14 days, hepatic maturation was induced for additional 14 days by application of 1.7% DMSO (Sigma) to the culture medium. .. Human epithelial lung carcinoma cells (A549; ATCC, Manassas, VA, USA) were cultured using Dulbecco´s Modified Eagle Medium (DMEM) high glucose (Biowest) supplemented with 10% fetal bovine serum (FBS; c.c.pro), 2 mM l -Glutamine (Biowest) and 1% penicillin/streptomycin (P/S; Biowest). .. Human extracellular matrix (hECM) was isolated from a lung of a deceased patient.

Article Title: In Vitro Cytotoxicity Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells
Article Snippet: Given these GC-1 cell characteristics, they are a cell model with scientific relevance for male reproductive system studies as indicated by their use in relevant studies about the male reproductive system [ , ]. .. GC-1 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, bioWest, Riverside, MO, USA) supplemented with 10% (v /v ) of Fetal Bovine Serum (FBS; bioWest) and 1% (v /v ) penicillin-streptomycin solution (Pen Strep; bioWest). .. Cell culture was maintained at 37 °C and 5% CO2 atmosphere.

other:

Article Title: Isotopomer analysis of lipid biosynthesis by high resolution mass spectrometry and NMR
Article Snippet: RPMI 1640 medium and trypsin were purchased from Hyclone (Thermo Fisher Scientific, Waltham, MA), Biowest fetal bovine serum (FBS) from USA Scientific (Ocala, FL), and penicillin/streptomycin from Atlanta Biologicals (Lawrenceville, GA).

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    Biowest SAS dextran charcoal treated fbs
    Release of NO from JS-K . (A) Generation of NO from JS-K in <t>RPMI-1640</t> medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.
    Dextran Charcoal Treated Fbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biowest SAS fbs
    MSC cumulative population doublings calculated from P1 to P3 in culture conditions <t>DMEM</t> 10% <t>FBS</t> versus DMEM 5% PL. n = 12. Data presented as mean ± SD. * p
    Fbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fbs - by Bioz Stars, 2021-04
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    86
    Biowest SAS fetal bovine serum fbs
    Release of NO from JS-K . (A) Generation of NO from JS-K in <t>RPMI-1640</t> medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.
    Fetal Bovine Serum Fbs, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal bovine serum fbs/product/Biowest SAS
    Average 86 stars, based on 1 article reviews
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    fetal bovine serum fbs - by Bioz Stars, 2021-04
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    Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.

    Journal: BMC Cancer

    Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells

    doi: 10.1186/1471-2407-12-130

    Figure Lengend Snippet: Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.

    Article Snippet: LNCaP-SSR were routinely grown in RPMI-1640, 1% Penicillin/Streptomycin supplemented with 10% steroid free, dextran charcoal treated FBS (FBSdcc, BioWest, Nuaille, France) [ ].

    Techniques: Fluorescence, Microscopy, Staining

    MSC cumulative population doublings calculated from P1 to P3 in culture conditions DMEM 10% FBS versus DMEM 5% PL. n = 12. Data presented as mean ± SD. * p

    Journal: Stem Cell Research & Therapy

    Article Title: Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience

    doi: 10.1186/s13287-018-0863-8

    Figure Lengend Snippet: MSC cumulative population doublings calculated from P1 to P3 in culture conditions DMEM 10% FBS versus DMEM 5% PL. n = 12. Data presented as mean ± SD. * p

    Article Snippet: Isolation and in-vitro expansion of BM-MSCs BMNCs were harvested from the iliac crest of 12 donors (mean age 25) and equally split into two cellular culture conditions at a seeding density of 10,000 cells/cm2 [ ]: Dulbecco’s modified Eagle medium high glucose (DMEM) (Invitrogen, Thermofisher) containing 10% of FBS (BioWest); and DMEM containing 5% PL and heparin 40 IU/ml (EPSOCLAR 5000 U.I./5 ml).

    Techniques:

    Morphological changes induced by PL (representative case). a When observed by inverted microscope (10×), MCSs cultivated in presence of PL appear smaller, more refractive and less attached to plastic support than MCSs cultured with FBS. b Morphological changes confirmed by flow cytometry analysis of FSC and SSC, as cells cultured in PL showed reduction of FSC and SSC rate representative of less complexity and smaller dimensions. DMEM Dulbecco’s modified Eagle medium, FBS fetal bovine serum, FSC forward scatter, MSC mesenchymal stromal/stem cell, PL platelet lysate, SSC side scatter

    Journal: Stem Cell Research & Therapy

    Article Title: Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience

    doi: 10.1186/s13287-018-0863-8

    Figure Lengend Snippet: Morphological changes induced by PL (representative case). a When observed by inverted microscope (10×), MCSs cultivated in presence of PL appear smaller, more refractive and less attached to plastic support than MCSs cultured with FBS. b Morphological changes confirmed by flow cytometry analysis of FSC and SSC, as cells cultured in PL showed reduction of FSC and SSC rate representative of less complexity and smaller dimensions. DMEM Dulbecco’s modified Eagle medium, FBS fetal bovine serum, FSC forward scatter, MSC mesenchymal stromal/stem cell, PL platelet lysate, SSC side scatter

    Article Snippet: Isolation and in-vitro expansion of BM-MSCs BMNCs were harvested from the iliac crest of 12 donors (mean age 25) and equally split into two cellular culture conditions at a seeding density of 10,000 cells/cm2 [ ]: Dulbecco’s modified Eagle medium high glucose (DMEM) (Invitrogen, Thermofisher) containing 10% of FBS (BioWest); and DMEM containing 5% PL and heparin 40 IU/ml (EPSOCLAR 5000 U.I./5 ml).

    Techniques: Inverted Microscopy, Cell Culture, Flow Cytometry, Cytometry, Modification

    NEFAs directly reduce Mg 2+ concentrations. Linear regression analyses of Mg 2+ and NEFA concentration in ( a ) BSA dissolved in 1 mmol/l MgCl 2 ( y = −0.12 x + 0.83, r 2 = 0.97, p ≤ 0.05), ( b ) FBS ( y = −0.10 x + 1.36, r 2 = 0.90, p ≤ 0.05) and ( c ) MgCl 2 solution ( y = −0.08 x + 1.00, r 2 = 0.99, p ≤ 0.05). Results from one representative experiment are shown. The experiment was repeated three additional times with similar results

    Journal: Diabetologia

    Article Title: Increased NEFA levels reduce blood Mg2+ in hypertriacylglycerolaemic states via direct binding of NEFA to Mg2+

    doi: 10.1007/s00125-018-4771-3

    Figure Lengend Snippet: NEFAs directly reduce Mg 2+ concentrations. Linear regression analyses of Mg 2+ and NEFA concentration in ( a ) BSA dissolved in 1 mmol/l MgCl 2 ( y = −0.12 x + 0.83, r 2 = 0.97, p ≤ 0.05), ( b ) FBS ( y = −0.10 x + 1.36, r 2 = 0.90, p ≤ 0.05) and ( c ) MgCl 2 solution ( y = −0.08 x + 1.00, r 2 = 0.99, p ≤ 0.05). Results from one representative experiment are shown. The experiment was repeated three additional times with similar results

    Article Snippet: Extracted NEFAs were added to 250 μl FBS (Biowest, Nuaillé, France), 1 mmol/l MgCl2 , or 0.5 mmol/l BSA in the amounts of 0, 25, 50, 100, 200, 350, 500 and 700 μl.

    Techniques: Concentration Assay

    Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.

    Journal: BMC Cancer

    Article Title: JS-K, a glutathione/glutathione S-transferase-activated nitric oxide releasing prodrug inhibits androgen receptor and WNT-signaling in prostate cancer cells

    doi: 10.1186/1471-2407-12-130

    Figure Lengend Snippet: Release of NO from JS-K . (A) Generation of NO from JS-K in RPMI-1640 medium containing 10% fetal bovine serum was determined indirectly by photorimetric detection of nitrite according to Green et al. [ 31 ]. (B) Detection of nitrotyrosine by fluorescence microscopy in 22Rv1 cells: Generation of intracellular NO was detected indirectly by detection of nitrotyrosine (green). Cell nuclei were stained with DAPI. [A] untreated cells, [B] 2 μM JS-K neg , [C] 2 μM JS-K, [D] 200 μM DETA/NO.

    Article Snippet: 22Rv1, LNCaP, DU-145 and PC-3 were grown in RPMI-1640 (PAA Laboratories GmbH, Pasching, Austria) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin (BioWest, Nuaille, France).

    Techniques: Fluorescence, Microscopy, Staining