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Atlanta Biologicals fetal bovine serum fbs
Ellman Assay of <t>FBS</t> and lysate of <t>A549</t> cells treated with ITC or ISC. Shown is the percentage of free SH compared to FBS control (without drugs). (A) Ellman assay of FBS treated with 400 μM ITC or ISC over 6 h (n=3). (B) Ellman assay of A549 cell
Fetal Bovine Serum Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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1) Product Images from "Phenylalkyl isoselenocyanates vs phenylalkyl isothiocyanates: thiol reactivity and its implications"

Article Title: Phenylalkyl isoselenocyanates vs phenylalkyl isothiocyanates: thiol reactivity and its implications

Journal: Chemico-biological interactions

doi: 10.1016/j.cbi.2012.08.022

Ellman Assay of FBS and lysate of A549 cells treated with ITC or ISC. Shown is the percentage of free SH compared to FBS control (without drugs). (A) Ellman assay of FBS treated with 400 μM ITC or ISC over 6 h (n=3). (B) Ellman assay of A549 cell
Figure Legend Snippet: Ellman Assay of FBS and lysate of A549 cells treated with ITC or ISC. Shown is the percentage of free SH compared to FBS control (without drugs). (A) Ellman assay of FBS treated with 400 μM ITC or ISC over 6 h (n=3). (B) Ellman assay of A549 cell

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Article Title: Dynamic compaction of human mesenchymal stem/precursor cells (MSC) into spheres self-activates caspase-dependent IL1 signaling to enhance secretion of modulators of inflammation and immunity (PGE2, TSG6 and STC1)
Article Snippet: MSC were suspended in complete culture medium (CCM) consisting of α-Minimum Essential Medium (MEM, Gibco), 17% fetal bovine serum (FBS, Atlanta Biologicals), 100 units/ml penicillin (Gibco), 100 μg/ml streptomycin (Gibco), and 2 mM L-glutamine (Gibco) and plated in a 152 cm2 culture dish (Corning).

Article Title: Combination of Nanogel Polyethylene glycol-Polyethylenimine and 6 (hydroxymethyl)-1,4-anthracenedione as an Anticancer Nanomedicine
Article Snippet: Cell culturePan 02 cells were maintained in medium containing RPMI 1640 (Invitrogen), 10% fetal bovine serum (FBS, Atlanta Biologicals), and 1x pen/strep (Invitrogen) at 37°C in a humidified atmosphere containing 5% carbon dioxide.

Article Title: The epigenetic reader Brd4 is required for osteoblast differentiation.
Article Snippet: Transcription networks and epigenetic mechanisms including DNA methylation, histone modifications, and noncoding RNAs control lineage commitment of multipotent mesenchymal progenitor cells.

Article Title: Stiffening of Human Mesenchymal Stem Cell Spheroid Microenvironments Induced by Incorporation of Gelatin Microparticles
Article Snippet: Briefly, hMSCs were maintained on tissue culture polystyrene at sub-confluence in complete culture medium (CCM, Minimimal Essential Medium alpha (Invitrogen/GIBCO) supplemented with 16% fetal bovine serum (FBS, Atlanta Biologicals), 2 mM L-glutamine (Invitrogen/GIBCO), 100U/mL penicillin and 100 μg/mL streptomycin (Invitrogen/GIBCO).

Article Title: Neuron-to-Astrocyte Transition: Phenotypic Fluidity and the Formation of Hybrid “Asterons” in Differentiating Neurospheres
Article Snippet: Cells were then plated into standard T25 tissue culture flasks in growth medium consisting of DMEM/F12 containing N2 supplements, 5% fetal bovine serum (FBS; Atlanta Biologicals), 20ng/mL epidermal growth factor (EGF; Sigma, St. Louis), and 10ng/mL basic fibroblast growth factor (bFGF; Sigma).

Article Title: Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies
Article Snippet: The SCM was prepared using RPMI-1640 and all the aforementioned supplements along with 10% (v/v) fetal bovine serum (FBS, Atlanta Biologicals).

Article Title: Methoxychlor reduces estradiol levels by altering steroidogenesis and metabolism in mouse antral follicles in vitro
Article Snippet: Fetal bovine serum (5% FBS) was obtained from Atlanta Biologicals (Lawrenceville, GA).

Modification:

Article Title: Immunotherapeutic Potential of Anti-Human Endogenous Retrovirus-K Envelope Protein Antibodies in Targeting Breast Tumors
Article Snippet: The IDC51N, IDC51T, and IDCm73T cells were cultured from breast cancer patient tissues. .. The breast cancer cells and HEK293T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Invitrogen, Carlsbad, CA) supplemented with penicillin (100 U/mL) (Invitrogen), streptomycin (100 μg/mL) (Invitrogen), and fetal bovine serum (FBS; 10%) (Atlanta Biologicals, Lawrenceville, GA), which is the complete DMEM. ..

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    Atlanta Biologicals fbs
    Effect of chemokine concentration on cell entrance and exit from microchannels. A range of <t>FBS</t> concentrations (0%, 1%, 5% and 10%) were tested for the ability to induce cells to enter and migrate successfully through the full channel length (200 µm), and exit the microchannels. The rate of HOS cells that migrated into (a) and exited from (b) microchannels is expressed as percentage of the total number of cells seeded within a distance of 100 µm from the channel entrances. 10% FBS was used to examine the migration rate of MDA-MB-231 and <t>MCF-10A</t> that entered (c) and exited (d) the 200 µm long microchannels in order to compare their respective invasive potential.
    Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs/product/Atlanta Biologicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fbs - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Atlanta Biologicals tet tested fetal bovine serum
    Experimental outline and validation of <t>tet-inducible</t> expression system. (A) Representative <t>NB2a/d1</t> cells at day 3 and 12 after initiation of dbcAMP-induced differentiation, transiently transfected with GFP-H on day 2 after initiation of differentiation. Insets show higher-magnification views of regions of axonal neurites indicated by arrows. Note association of GFP with filamentous structures along the length of axonal neurites along with saturation of the soma. (B) Timeline of induction and cessation of GFP-H expression under control of the tet-inducible promoter by addition of doxycycline (dox) for 12 h after 24 h or 72 h of differentiation (‘Early-On’ and ‘Late-On’, respectively). Additional cultures did not receive dox (‘Leak’) or received dox for 1 week (‘Always-On’). (C) Quantification of GFP under the conditions described in panel B at 24 and 72 h after transfection ( n =total of > 1000 cells from three separate experiments; > 65 cells/condition/time point). * P
    Tet Tested Fetal Bovine Serum, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tet tested fetal bovine serum/product/Atlanta Biologicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tet tested fetal bovine serum - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Effect of chemokine concentration on cell entrance and exit from microchannels. A range of FBS concentrations (0%, 1%, 5% and 10%) were tested for the ability to induce cells to enter and migrate successfully through the full channel length (200 µm), and exit the microchannels. The rate of HOS cells that migrated into (a) and exited from (b) microchannels is expressed as percentage of the total number of cells seeded within a distance of 100 µm from the channel entrances. 10% FBS was used to examine the migration rate of MDA-MB-231 and MCF-10A that entered (c) and exited (d) the 200 µm long microchannels in order to compare their respective invasive potential.

    Journal: PLoS ONE

    Article Title: Chemotaxis of Cell Populations through Confined Spaces at Single-Cell Resolution

    doi: 10.1371/journal.pone.0029211

    Figure Lengend Snippet: Effect of chemokine concentration on cell entrance and exit from microchannels. A range of FBS concentrations (0%, 1%, 5% and 10%) were tested for the ability to induce cells to enter and migrate successfully through the full channel length (200 µm), and exit the microchannels. The rate of HOS cells that migrated into (a) and exited from (b) microchannels is expressed as percentage of the total number of cells seeded within a distance of 100 µm from the channel entrances. 10% FBS was used to examine the migration rate of MDA-MB-231 and MCF-10A that entered (c) and exited (d) the 200 µm long microchannels in order to compare their respective invasive potential.

    Article Snippet: MCF-7 and MDA-MB-231 cells were cultured in DMEM supplemented with 10% FBS (Atlanta Biologicals) and 1% Penicillin-Streptomycin.

    Techniques: Concentration Assay, Migration, Multiple Displacement Amplification

    (A) Oxidative stress induced Sumo1 conjugation in hLECs, and these cells displayed higher levels of ROS and reduced viability. Cultured hLECs were treated with different concentrations of H 2 O 2 for 30 min. Complete medium (DMEM supplemented with 15% FBS) was replaced with DMEM containing 0.2% BSA prior to the H 2 O 2 treatment. (A, a). Total cell lysates were prepared and immunoblotted with anti-Sumo1 antibody to measure free Sumo1 and Sumo1 conjugates. (A, b). Cells were cultured and subjected to oxidative stress. ROS levels were monitored. (A, c). MTS assay was conducted to monitor cell viability against oxidative stress. Data represent means ± SD of three independent experiments. * p

    Journal: The FEBS journal

    Article Title: Aberrant Sumoylation Signaling Evoked by Reactive Oxygen Species Impairs Protective Function of Prdx6 by Destabilization and Repression of its Transcription

    doi: 10.1111/febs.12866

    Figure Lengend Snippet: (A) Oxidative stress induced Sumo1 conjugation in hLECs, and these cells displayed higher levels of ROS and reduced viability. Cultured hLECs were treated with different concentrations of H 2 O 2 for 30 min. Complete medium (DMEM supplemented with 15% FBS) was replaced with DMEM containing 0.2% BSA prior to the H 2 O 2 treatment. (A, a). Total cell lysates were prepared and immunoblotted with anti-Sumo1 antibody to measure free Sumo1 and Sumo1 conjugates. (A, b). Cells were cultured and subjected to oxidative stress. ROS levels were monitored. (A, c). MTS assay was conducted to monitor cell viability against oxidative stress. Data represent means ± SD of three independent experiments. * p

    Article Snippet: LECs isolated from Prdx6 -targeted mutants ( Prdx6−/− ) and wild-type ( Prdx6+/+ ) mice were generated and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA) with 10% FBS (Atlanta Biologicals, Inc., Flowery Branch, GA, USA) as described earlier [ ].

    Techniques: Conjugation Assay, Cell Culture, MTS Assay

    Expression of a Rho-independent recombinant p120 in p120-depleted cells rescues TEER. HDMECs stably infected with lentivirus containing shNTS or shp120 were seeded at confluence. Seventy-two hours later, monolayers were infected with adenovirus containing GFP, p120 1A-GFP, or p120 4AK→A-GFP. A : changes in electrical resistance were measured from 2 h before infection to 48 h postinfection on an ECIS in a 8W10E plate. Values are means ± SE ( n = 6; 3 experiments in duplicate). B : 24 h after infection, cells were serum starved for 24 h (0.3% FBS in EBM-2 medium) before treatment with PBS containing calcium and magnesium (PBS, control) or thrombin (thr; 5 U/ml). Changes in electrical resistance were measured for 2 h after treatment on an ECIS in a 8W10E plate. Values are means ± SE ( n = 6; 3 experiments in duplicate).

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: p120 regulates endothelial permeability independently of its NH2 terminus and Rho binding

    doi: 10.1152/ajpheart.00812.2010

    Figure Lengend Snippet: Expression of a Rho-independent recombinant p120 in p120-depleted cells rescues TEER. HDMECs stably infected with lentivirus containing shNTS or shp120 were seeded at confluence. Seventy-two hours later, monolayers were infected with adenovirus containing GFP, p120 1A-GFP, or p120 4AK→A-GFP. A : changes in electrical resistance were measured from 2 h before infection to 48 h postinfection on an ECIS in a 8W10E plate. Values are means ± SE ( n = 6; 3 experiments in duplicate). B : 24 h after infection, cells were serum starved for 24 h (0.3% FBS in EBM-2 medium) before treatment with PBS containing calcium and magnesium (PBS, control) or thrombin (thr; 5 U/ml). Changes in electrical resistance were measured for 2 h after treatment on an ECIS in a 8W10E plate. Values are means ± SE ( n = 6; 3 experiments in duplicate).

    Article Snippet: For thrombin treatments, cells were serum starved for 24 h in 0.3% FBS (Atlanta Biologicals, Lawrenceville, GA) in EBM-2 medium before treatment with vehicle control [PBS containing calcium and magnesium (PBS+)] or 5 U/ml thrombin (Sigma, St. Louis, MO).

    Techniques: Expressing, Recombinant, Stable Transfection, Infection, Electric Cell-substrate Impedance Sensing

    Experimental outline and validation of tet-inducible expression system. (A) Representative NB2a/d1 cells at day 3 and 12 after initiation of dbcAMP-induced differentiation, transiently transfected with GFP-H on day 2 after initiation of differentiation. Insets show higher-magnification views of regions of axonal neurites indicated by arrows. Note association of GFP with filamentous structures along the length of axonal neurites along with saturation of the soma. (B) Timeline of induction and cessation of GFP-H expression under control of the tet-inducible promoter by addition of doxycycline (dox) for 12 h after 24 h or 72 h of differentiation (‘Early-On’ and ‘Late-On’, respectively). Additional cultures did not receive dox (‘Leak’) or received dox for 1 week (‘Always-On’). (C) Quantification of GFP under the conditions described in panel B at 24 and 72 h after transfection ( n =total of > 1000 cells from three separate experiments; > 65 cells/condition/time point). * P

    Journal: Biology Open

    Article Title: Assembly and turnover of neurofilaments in growing axonal neurites

    doi: 10.1242/bio.028795

    Figure Lengend Snippet: Experimental outline and validation of tet-inducible expression system. (A) Representative NB2a/d1 cells at day 3 and 12 after initiation of dbcAMP-induced differentiation, transiently transfected with GFP-H on day 2 after initiation of differentiation. Insets show higher-magnification views of regions of axonal neurites indicated by arrows. Note association of GFP with filamentous structures along the length of axonal neurites along with saturation of the soma. (B) Timeline of induction and cessation of GFP-H expression under control of the tet-inducible promoter by addition of doxycycline (dox) for 12 h after 24 h or 72 h of differentiation (‘Early-On’ and ‘Late-On’, respectively). Additional cultures did not receive dox (‘Leak’) or received dox for 1 week (‘Always-On’). (C) Quantification of GFP under the conditions described in panel B at 24 and 72 h after transfection ( n =total of > 1000 cells from three separate experiments; > 65 cells/condition/time point). * P

    Article Snippet: Cell culture and transfection Mouse NB2a/d1 neuroblastoma cells were maintained in high-glucose DMEM supplemented with 10% tet-tested fetal bovine serum (Atlanta Biologicals, Flowery Branch, GA, USA), 2 mM L-glutamine, and antibiotic-antimycotic solution (‘complete DMEM’) and differentiated for 20 h with 1 mM dibutyrl-cyclic-AMP ( ).

    Techniques: Expressing, Transfection