fetal bovine serum fbs  (ATCC)


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    Name:
    Fetal Bovine Serum FBS
    Description:
    Triple filtered through 0 1 μm filters Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States
    Catalog Number:
    30-2021
    Price:
    None
    Applications:
    Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.
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    Structured Review

    ATCC fetal bovine serum fbs
    High glucose (HG) and diabetes increase TNF-α production and PKC activity in BM-derived macrophages (A–C). A: Rat BM-derived macrophages were cultured under control glucose (CG, 5.6 mM) or HG (25 mM) for 72 h. TNF-α production was measured in conditioned medium (n=17). B: PKC activity in rat BM-derived macrophages stimulated with HG for 72 h or PMA (100 nM) for 30 min (n=3). C: PKC activity in BM-derived macrophages isolated from control and diabetic mice at 12 weeks of diabetes (n=4, also indicates the number of mice studied individually). Diabetes was induced in 6-week-old male C57Bl6/J mice fasted for 12 h, with intraperitoneal injections of STZ in citrate buffer (90 mg/kg) for 2 consecutive days. D: β2AR agonists decreased LPS-induced TNF-α production in rat BM-derived macrophages. Cells were preincubated with metaproterenol or terbutaline hemisulfate for 1 h and then stimulated with LPS (50 ng/mL) for 6 or 48 h (n=3). E: β2AR agonists decreased diabetes-induced TNF-α production in rat PBMCs isolated from control and diabetic rats at 4 weeks of diabetes. Cells were incubated in <t>RPMI</t> medium with 10% <t>FBS</t> for 1 h, and then treated with 500 nM metaproterenol or terbutaline hemisulfate for an additional 16 h with or without 1 h pretreatment with ICI118551 (100 nM), a selective β2AR blocker (n=9~11, also indicates the number of rats studied individually). * p
    Triple filtered through 0 1 μm filters Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States
    https://www.bioz.com/result/fetal bovine serum fbs/product/ATCC
    Average 94 stars, based on 274 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum fbs - by Bioz Stars, 2020-07
    94/100 stars

    Images

    1) Product Images from "Beta 2 adrenergic receptor agonists are novel regulators of macrophage activation in diabetic renal and cardiovascular complications"

    Article Title: Beta 2 adrenergic receptor agonists are novel regulators of macrophage activation in diabetic renal and cardiovascular complications

    Journal: Kidney international

    doi: 10.1016/j.kint.2017.02.013

    High glucose (HG) and diabetes increase TNF-α production and PKC activity in BM-derived macrophages (A–C). A: Rat BM-derived macrophages were cultured under control glucose (CG, 5.6 mM) or HG (25 mM) for 72 h. TNF-α production was measured in conditioned medium (n=17). B: PKC activity in rat BM-derived macrophages stimulated with HG for 72 h or PMA (100 nM) for 30 min (n=3). C: PKC activity in BM-derived macrophages isolated from control and diabetic mice at 12 weeks of diabetes (n=4, also indicates the number of mice studied individually). Diabetes was induced in 6-week-old male C57Bl6/J mice fasted for 12 h, with intraperitoneal injections of STZ in citrate buffer (90 mg/kg) for 2 consecutive days. D: β2AR agonists decreased LPS-induced TNF-α production in rat BM-derived macrophages. Cells were preincubated with metaproterenol or terbutaline hemisulfate for 1 h and then stimulated with LPS (50 ng/mL) for 6 or 48 h (n=3). E: β2AR agonists decreased diabetes-induced TNF-α production in rat PBMCs isolated from control and diabetic rats at 4 weeks of diabetes. Cells were incubated in RPMI medium with 10% FBS for 1 h, and then treated with 500 nM metaproterenol or terbutaline hemisulfate for an additional 16 h with or without 1 h pretreatment with ICI118551 (100 nM), a selective β2AR blocker (n=9~11, also indicates the number of rats studied individually). * p
    Figure Legend Snippet: High glucose (HG) and diabetes increase TNF-α production and PKC activity in BM-derived macrophages (A–C). A: Rat BM-derived macrophages were cultured under control glucose (CG, 5.6 mM) or HG (25 mM) for 72 h. TNF-α production was measured in conditioned medium (n=17). B: PKC activity in rat BM-derived macrophages stimulated with HG for 72 h or PMA (100 nM) for 30 min (n=3). C: PKC activity in BM-derived macrophages isolated from control and diabetic mice at 12 weeks of diabetes (n=4, also indicates the number of mice studied individually). Diabetes was induced in 6-week-old male C57Bl6/J mice fasted for 12 h, with intraperitoneal injections of STZ in citrate buffer (90 mg/kg) for 2 consecutive days. D: β2AR agonists decreased LPS-induced TNF-α production in rat BM-derived macrophages. Cells were preincubated with metaproterenol or terbutaline hemisulfate for 1 h and then stimulated with LPS (50 ng/mL) for 6 or 48 h (n=3). E: β2AR agonists decreased diabetes-induced TNF-α production in rat PBMCs isolated from control and diabetic rats at 4 weeks of diabetes. Cells were incubated in RPMI medium with 10% FBS for 1 h, and then treated with 500 nM metaproterenol or terbutaline hemisulfate for an additional 16 h with or without 1 h pretreatment with ICI118551 (100 nM), a selective β2AR blocker (n=9~11, also indicates the number of rats studied individually). * p

    Techniques Used: Activity Assay, Derivative Assay, Cell Culture, Isolation, Mouse Assay, Incubation

    2) Product Images from "A New Method for Delivering a Hydrophobic Drug for Photodynamic Therapy Using Pure Nanocrystal Form of the Drug"

    Article Title: A New Method for Delivering a Hydrophobic Drug for Photodynamic Therapy Using Pure Nanocrystal Form of the Drug

    Journal: Molecular pharmaceutics

    doi: 10.1021/mp060117f

    (A) Fluorescence signal from HPPH nanocrystals (4 μM) in pure cell culture medium (MEMα) and MEMα with added FBS or BSA after 6 hrs of incubation at room temperature; (B) Time-dependent fluorescence signal recovery from HPPH nanocrystals
    Figure Legend Snippet: (A) Fluorescence signal from HPPH nanocrystals (4 μM) in pure cell culture medium (MEMα) and MEMα with added FBS or BSA after 6 hrs of incubation at room temperature; (B) Time-dependent fluorescence signal recovery from HPPH nanocrystals

    Techniques Used: Fluorescence, Cell Culture, Incubation

    3) Product Images from "Macrolactone Nuiapolide, Isolated from a Hawaiian Marine Cyanobacterium, Exhibits Anti-Chemotactic Activity"

    Article Title: Macrolactone Nuiapolide, Isolated from a Hawaiian Marine Cyanobacterium, Exhibits Anti-Chemotactic Activity

    Journal: Marine Drugs

    doi: 10.3390/md13106274

    Evaluation of Jurkat cultures in the Boyden chamber assay. Images of microscope fields of the bottom well after 24 h of incubation. ( a ) Bottom well received no FBS and no test substance; ( b ) Bottom well received 10% FBS and no test substance; ( c ) Bottom well received 10% FBS and FTY720 (5 µg/mL); ( d ) Bottom well received 10% FBS and 13-F10 extract (25 µg/mL). Shrinkage of cells in ( c ) is indicative of cell death.
    Figure Legend Snippet: Evaluation of Jurkat cultures in the Boyden chamber assay. Images of microscope fields of the bottom well after 24 h of incubation. ( a ) Bottom well received no FBS and no test substance; ( b ) Bottom well received 10% FBS and no test substance; ( c ) Bottom well received 10% FBS and FTY720 (5 µg/mL); ( d ) Bottom well received 10% FBS and 13-F10 extract (25 µg/mL). Shrinkage of cells in ( c ) is indicative of cell death.

    Techniques Used: Boyden Chamber Assay, Microscopy, Incubation

    4) Product Images from "Lack of intracellular replication of M. tuberculosis and M. bovis BCG caused by delivering bacilli to lysosomes in murine brain microvascular endothelial cells"

    Article Title: Lack of intracellular replication of M. tuberculosis and M. bovis BCG caused by delivering bacilli to lysosomes in murine brain microvascular endothelial cells

    Journal: Oncotarget

    doi:

    Mtb and BCG bacilli cannot spread between BMECs in a monolayer Cells were infected with fluorescent Mtb or BCG bacilli (MOI = 0.1) for 24 h, then washed three times with PBS to remove unbound bacteria. The cells were overlaid with 1% agarose/DMEM with 1% FBS, with or without amikacin (50 μg/ml) and cultured for up to 7 days. Extracellular bacterial replication was observed in amikacin-free infections with Mtb and BCG. However, plaque formation, indicating bacterial spread and cytotoxicity, was only found in Mtb -infected cells in the absence of amikacin. Infections were performed in triplicate and experiments repeated two times.
    Figure Legend Snippet: Mtb and BCG bacilli cannot spread between BMECs in a monolayer Cells were infected with fluorescent Mtb or BCG bacilli (MOI = 0.1) for 24 h, then washed three times with PBS to remove unbound bacteria. The cells were overlaid with 1% agarose/DMEM with 1% FBS, with or without amikacin (50 μg/ml) and cultured for up to 7 days. Extracellular bacterial replication was observed in amikacin-free infections with Mtb and BCG. However, plaque formation, indicating bacterial spread and cytotoxicity, was only found in Mtb -infected cells in the absence of amikacin. Infections were performed in triplicate and experiments repeated two times.

    Techniques Used: Infection, Cell Culture

    5) Product Images from "Multidimensional hydrogel models reveal endothelial network angiocrine signals increase glioblastoma cell number, invasion, and temozolomide resistance"

    Article Title: Multidimensional hydrogel models reveal endothelial network angiocrine signals increase glioblastoma cell number, invasion, and temozolomide resistance

    Journal: bioRxiv

    doi: 10.1101/2020.01.18.911396

    Migration was assessed in response to the presence of each protein loaded into the test channel of the microfluidic assay. The negative control was 1:1 DMEM + 2% FBS:EBM2 + 2% FBS alone. Proteins tested included those whose genes are upregulated in glioblastoma in addition to IL8, which has been shown to induce the migration of primary GBM cells. TIMP1 induced increases in the number of cells (A) and the average invasion distance (B) of cells migrating into the central gel channel. Data is normalized to the Negative group. * p
    Figure Legend Snippet: Migration was assessed in response to the presence of each protein loaded into the test channel of the microfluidic assay. The negative control was 1:1 DMEM + 2% FBS:EBM2 + 2% FBS alone. Proteins tested included those whose genes are upregulated in glioblastoma in addition to IL8, which has been shown to induce the migration of primary GBM cells. TIMP1 induced increases in the number of cells (A) and the average invasion distance (B) of cells migrating into the central gel channel. Data is normalized to the Negative group. * p

    Techniques Used: Migration, Negative Control

    6) Product Images from "The First Scale-Up Production of Theranostic Nanoemulsions"

    Article Title: The First Scale-Up Production of Theranostic Nanoemulsions

    Journal: BioResearch Open Access

    doi: 10.1089/biores.2014.0030

    Nanoemulsion stability in biological media measured by dynamic light scattering (DLS) for small-scale nanoemulsions with/without a drug (A, B) , medium scale (C, D) , and large scale (E, F) . Nanoemulsions were diluted at 1/40 (v/v) in deionized water, serum-free Dulbecco's modified Eagle's medium (DMEM) cell culture media, 10% fetal bovine serum (FBS), and 20% FBS DMEM and incubated for up to 72 h at body temperature (37°C). Samples were tested by DLS for size and polydispersity at times 0, 24, 48, and 72 h. Data represent average droplet size (Z-average) with standard deviation as half of polydispersity width. Incubation samples were measured by DLS, undiluted.
    Figure Legend Snippet: Nanoemulsion stability in biological media measured by dynamic light scattering (DLS) for small-scale nanoemulsions with/without a drug (A, B) , medium scale (C, D) , and large scale (E, F) . Nanoemulsions were diluted at 1/40 (v/v) in deionized water, serum-free Dulbecco's modified Eagle's medium (DMEM) cell culture media, 10% fetal bovine serum (FBS), and 20% FBS DMEM and incubated for up to 72 h at body temperature (37°C). Samples were tested by DLS for size and polydispersity at times 0, 24, 48, and 72 h. Data represent average droplet size (Z-average) with standard deviation as half of polydispersity width. Incubation samples were measured by DLS, undiluted.

    Techniques Used: Modification, Cell Culture, Incubation, Standard Deviation

    Related Articles

    Incubation:

    Article Title: Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions
    Article Snippet: .. Individual 50 ml conical tubes were filled with 10 ml of cell culture medium [F12K medium (ATCC® ) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (ATCC® )], inoculated with 0.1 ml (1:100 for mid-log cultures), 0.25 ml (1:40 for late-log phase cultures) and 1 ml (1:10 for stationary phase cultures) of a saturated culture of B. melitensis 16 M and incubated overnight at 37°C with 5% CO2 , loose lids and shaking (200 rpm). ..

    Cell Culture:

    Article Title: Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions
    Article Snippet: .. Individual 50 ml conical tubes were filled with 10 ml of cell culture medium [F12K medium (ATCC® ) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (ATCC® )], inoculated with 0.1 ml (1:100 for mid-log cultures), 0.25 ml (1:40 for late-log phase cultures) and 1 ml (1:10 for stationary phase cultures) of a saturated culture of B. melitensis 16 M and incubated overnight at 37°C with 5% CO2 , loose lids and shaking (200 rpm). ..

    Article Title: Beta 2 adrenergic receptor agonists are novel regulators of macrophage activation in diabetic renal and cardiovascular complications
    Article Snippet: .. Cells were cultured in RPMI containing 10% fetal bovine serum (FBS) and 20% L929 cell (American Type Culture Collection [ATCC], Manassas, VA) conditioned media as a source of macrophage colony stimulating factor . .. After 7 days, at least 97% of the cells were double positive for CD68 (Serotec, Oxford, UK) and CD11b/c (BD Biosciences, San Diego, CA).

    Article Title: FOXM1 is a downstream target of LPA and YAP oncogenic signaling pathways in high grade serous ovarian cancer
    Article Snippet: .. CAOV3 were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), and 100 μg/mL P/S. .. OVCAR5 cells were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), 0.1 mM MEM Non-Essential Amino Acids (NEAA), 2 mM L-glutamine and 100 μg/mL P/S.

    Article Title: FOXM1 is a downstream target of LPA and YAP oncogenic signaling pathways in high grade serous ovarian cancer
    Article Snippet: .. OVCAR5 cells were cultured in DMEM with glutamine, 10% FBS (ATCC, Manassas, VA), 0.1 mM MEM Non-Essential Amino Acids (NEAA), 2 mM L-glutamine and 100 μg/mL P/S. .. For serum starvation, cells were incubated in the basal medium without FBS or antibiotics.

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  • tf 1a  (ATCC)
    93
    ATCC tf 1a
    <t>TF-1a</t> cells stained with DIPP Quick Stains. TF-1a cells were treated with PMA for 48 hours, after which the cells were collected and slides were prepared following the method described in Section 2 . The staining method followed manufacture's instruction (×1000).
    Tf 1a, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 3 article reviews
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    91
    ATCC fibroblasts fbs
    Fibroblast gene expression is impacted due to co-culture with GDNF-overexpressing transgenic <t>SCs.</t> Fibroblasts <t>(FBs)</t> were cultured with transgenic SCs, with or without GDNF expression. ECM FB gene expression was increased when FBs were cultured with GDNF-overexpressing transgenic SCs expressing GDNF. Data represented as means with standard deviation error bars (n=4) and letters indicate which groups were not statistically different from one another (p > 0.05; each group has the same letter) or statistically different from one another (p
    Fibroblasts Fbs, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    fibroblasts fbs - by Bioz Stars, 2020-07
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    85
    ATCC wm 266 4 melanoma cells
    Chemically induced hypoxia increases secretion of hUcn 2-ir from endogenously expressing <t>WM-266-4</t> melanoma cells. A, Conditioned medium was collected from untreated or DMOG-treated cells after 24 and 48 hours. Media were analyzed for total hUcn 2-ir by
    Wm 266 4 Melanoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    wm 266 4 melanoma cells - by Bioz Stars, 2020-07
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    Image Search Results


    TF-1a cells stained with DIPP Quick Stains. TF-1a cells were treated with PMA for 48 hours, after which the cells were collected and slides were prepared following the method described in Section 2 . The staining method followed manufacture's instruction (×1000).

    Journal: International Journal of Cell Biology

    Article Title: A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips

    doi: 10.1155/2015/813216

    Figure Lengend Snippet: TF-1a cells stained with DIPP Quick Stains. TF-1a cells were treated with PMA for 48 hours, after which the cells were collected and slides were prepared following the method described in Section 2 . The staining method followed manufacture's instruction (×1000).

    Article Snippet: Preparation of Slides and Glass Spreader Human myeloid leukemia cells lines, TF-1a, TF-1, and MV4-11, were purchased from ATCC (Rockville, MD) and maintained in RPMI 1640 (TF-1a and TF-1) or IMDM (MV4-11) supplemented with 10% FBS without (TF-1a) or with GM-CSF (5 ng/mL, TF-1, MV4-11) at 37°C in humidified air containing 5% CO2 .

    Techniques: Staining

    PMA induces macrophage-like differentiation. TF-1a cells were treated with PMA for 72 hours, after which the cells were collected and slides were prepared and then stained following the protocol described in Section 2 . (a) Giemsa staining; (b) (×1000): methylene blue/eosin staining (×1000).

    Journal: International Journal of Cell Biology

    Article Title: A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips

    doi: 10.1155/2015/813216

    Figure Lengend Snippet: PMA induces macrophage-like differentiation. TF-1a cells were treated with PMA for 72 hours, after which the cells were collected and slides were prepared and then stained following the protocol described in Section 2 . (a) Giemsa staining; (b) (×1000): methylene blue/eosin staining (×1000).

    Article Snippet: Preparation of Slides and Glass Spreader Human myeloid leukemia cells lines, TF-1a, TF-1, and MV4-11, were purchased from ATCC (Rockville, MD) and maintained in RPMI 1640 (TF-1a and TF-1) or IMDM (MV4-11) supplemented with 10% FBS without (TF-1a) or with GM-CSF (5 ng/mL, TF-1, MV4-11) at 37°C in humidified air containing 5% CO2 .

    Techniques: Staining

    Fibroblast gene expression is impacted due to co-culture with GDNF-overexpressing transgenic SCs. Fibroblasts (FBs) were cultured with transgenic SCs, with or without GDNF expression. ECM FB gene expression was increased when FBs were cultured with GDNF-overexpressing transgenic SCs expressing GDNF. Data represented as means with standard deviation error bars (n=4) and letters indicate which groups were not statistically different from one another (p > 0.05; each group has the same letter) or statistically different from one another (p

    Journal: Biotechnology and bioengineering

    Article Title: Transgenic SCs expressing GDNF-IRES-DsRed impair nerve regeneration within cellular nerve allografts

    doi: 10.1002/bit.26335

    Figure Lengend Snippet: Fibroblast gene expression is impacted due to co-culture with GDNF-overexpressing transgenic SCs. Fibroblasts (FBs) were cultured with transgenic SCs, with or without GDNF expression. ECM FB gene expression was increased when FBs were cultured with GDNF-overexpressing transgenic SCs expressing GDNF. Data represented as means with standard deviation error bars (n=4) and letters indicate which groups were not statistically different from one another (p > 0.05; each group has the same letter) or statistically different from one another (p

    Article Snippet: Co-culture model systems of SC and fibroblasts (FBs) were generated using SCs, derived as previously described, with a rat FB cell line (CRL-1764, ATCC, Manassas, VA).

    Techniques: Expressing, Co-Culture Assay, Transgenic Assay, Cell Culture, Standard Deviation

    Chemically induced hypoxia increases secretion of hUcn 2-ir from endogenously expressing WM-266-4 melanoma cells. A, Conditioned medium was collected from untreated or DMOG-treated cells after 24 and 48 hours. Media were analyzed for total hUcn 2-ir by

    Journal: Endocrinology

    Article Title: Posttranslational Processing of Human and Mouse Urocortin 2: Characterization and Bioactivity of Gene Products

    doi: 10.1210/en.2012-2011

    Figure Lengend Snippet: Chemically induced hypoxia increases secretion of hUcn 2-ir from endogenously expressing WM-266-4 melanoma cells. A, Conditioned medium was collected from untreated or DMOG-treated cells after 24 and 48 hours. Media were analyzed for total hUcn 2-ir by

    Article Snippet: WM-266–4 melanoma cells (ATCC CRL-1676 were grown in MEM supplemented with 10% FBS.

    Techniques: Expressing

    A–C, Final reverse-phase HPLC of secretion medium from CHO-S cells transduced with mUcn 2 (A) and RINm cells transduced with hUcn 2 (B) and conditioned medium from WM-266-4 cells endogenously expressing hUcn 2 (C) shows a discrete single zone

    Journal: Endocrinology

    Article Title: Posttranslational Processing of Human and Mouse Urocortin 2: Characterization and Bioactivity of Gene Products

    doi: 10.1210/en.2012-2011

    Figure Lengend Snippet: A–C, Final reverse-phase HPLC of secretion medium from CHO-S cells transduced with mUcn 2 (A) and RINm cells transduced with hUcn 2 (B) and conditioned medium from WM-266-4 cells endogenously expressing hUcn 2 (C) shows a discrete single zone

    Article Snippet: WM-266–4 melanoma cells (ATCC CRL-1676 were grown in MEM supplemented with 10% FBS.

    Techniques: High Performance Liquid Chromatography, Transduction, Expressing

    FPLC gel permeation chromatography of secretion medium from A2058 human skin cells transduced with mUcn 2 or hUcn 2 lentivirus (A) and of conditioned medium from WM-266-4 human skin melanoma cells and of mouse skin extract (B) reveals Ucn 2 proteins.

    Journal: Endocrinology

    Article Title: Posttranslational Processing of Human and Mouse Urocortin 2: Characterization and Bioactivity of Gene Products

    doi: 10.1210/en.2012-2011

    Figure Lengend Snippet: FPLC gel permeation chromatography of secretion medium from A2058 human skin cells transduced with mUcn 2 or hUcn 2 lentivirus (A) and of conditioned medium from WM-266-4 human skin melanoma cells and of mouse skin extract (B) reveals Ucn 2 proteins.

    Article Snippet: WM-266–4 melanoma cells (ATCC CRL-1676 were grown in MEM supplemented with 10% FBS.

    Techniques: Fast Protein Liquid Chromatography, GPC Assay, Transduction