Journal: Biology of Sex Differences

Article Title: Sex differences in ferroptosis-related vulnerability to autism-like deficits in the adolescent medial prefrontal cortex following embryonic valproic acid exposure

doi: 10.1186/s13293-026-00982-x

Figure Lengend Snippet: E12.5 VPA exposed induces male-biased ferroptosis-associated alterations in the mPFC. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in mPFC. B-E Statistical graph of protein levels of GPX4, SLC7A11/xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 mPFC. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 mPFC measured. n = 3 per group. H The MDA level in the P24 mPFC. n = 3 per group. I The SOD concentration in the P24 mPFC. n = 3 per group. J Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11/xCT, n = 3 per group. K Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. L Representative immunofluorescence images of ROS (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Electron microscope of mitochondrial morphology in the P24 mPFC. The second row represents a partial enlargement of the first row, respectively. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: GPX4 (1:2000; Cat# A11243; Abclonal), FTH1 (1:5000; Cat# A19544; Abclonal), ACSL4 (1:1000; Cat#38493T; Cell Signaling Technology), xCT/SLC7A11 (1:1000; Cat# 28001T; Cell Signaling Technology), and β-actin (1:50000; Cat# 66009-1-Ig; Proteintech).

Techniques: Concentration Assay, Immunofluorescence, Fluorescence, Microscopy

Journal: Biology of Sex Differences

Article Title: Sex differences in ferroptosis-related vulnerability to autism-like deficits in the adolescent medial prefrontal cortex following embryonic valproic acid exposure

doi: 10.1186/s13293-026-00982-x

Figure Lengend Snippet: E12.5 VPA induces ferroptosis-related signatures in the hippocampus without sex differences. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in hippocampus. B-E Statistical graph of protein levels of GPX4, SLC7A11/ xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 hippocampus. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 hippocampus measured. n = 3 per group. H The MDA level in the P24 hippocampus. I The SOD concentration in the P24 hippocampus. J Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11, n = 3 per group. K Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. L Representative immunofluorescence images of ROS (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Electron microscope of mitochondrial morphology in the P24 hippocampus. The second row represents a partial enlargement of the first row, respectively. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: GPX4 (1:2000; Cat# A11243; Abclonal), FTH1 (1:5000; Cat# A19544; Abclonal), ACSL4 (1:1000; Cat#38493T; Cell Signaling Technology), xCT/SLC7A11 (1:1000; Cat# 28001T; Cell Signaling Technology), and β-actin (1:50000; Cat# 66009-1-Ig; Proteintech).

Techniques: Concentration Assay, Immunofluorescence, Fluorescence, Microscopy

Journal: Biology of Sex Differences

Article Title: Sex differences in ferroptosis-related vulnerability to autism-like deficits in the adolescent medial prefrontal cortex following embryonic valproic acid exposure

doi: 10.1186/s13293-026-00982-x

Figure Lengend Snippet: E12.5 VPA induces ferroptosis-related alterations in the striatum without sex differences. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in striatum. B-E Statistical graph of protein levels of GPX4, SLC7A11/xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 striatum. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 striatum measured. n = 3 per group. H The MDA level in the P24 striatum. I The SOD concentration in the P24 striatum. J Representative protein bands of GPX4, SLC7A11/xCT and FTH1 in mPFC, hippocampus and striatum. K-M Statistical graph of protein levels of GPX4, SLC7A11/xCT and FTH1. n = 5 per group. N Representative immunofluorescence images of SLC7A11/xCT (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11/xCT, n = 3 per group. O Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. P Representative immunofluorescence images of ROS (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of ROS, n = 3 per group. Q Electron microscope of mitochondrial morphology in the P24 striatum. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: GPX4 (1:2000; Cat# A11243; Abclonal), FTH1 (1:5000; Cat# A19544; Abclonal), ACSL4 (1:1000; Cat#38493T; Cell Signaling Technology), xCT/SLC7A11 (1:1000; Cat# 28001T; Cell Signaling Technology), and β-actin (1:50000; Cat# 66009-1-Ig; Proteintech).

Techniques: Concentration Assay, Immunofluorescence, Fluorescence, Microscopy

Journal: Biology of Sex Differences

Article Title: Sex differences in ferroptosis-related vulnerability to autism-like deficits in the adolescent medial prefrontal cortex following embryonic valproic acid exposure

doi: 10.1186/s13293-026-00982-x

Figure Lengend Snippet: Comparable Ferroptosis-Associated alteration in the mPFC Following E12.5 and PND14 VPA Exposure. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in mPFC. B-E Statistical graph of protein levels of GPX4, SLC7A11/ xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 mPFC. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 mPFC measured. n = 3 per group. H The MDA level in the P24 mPFC. n = 3 per group. I The SOD concentration in the P24 mPFC. n = 3 per group. J Observation of the morphology of brain nerve cells in the mPFC by HE staining. Scale bar = 100 μm. The second row shows Nissl staining of neuronal morphology in the mPFC by. Scale bar = 100 (raw)/20 (zoom) µm. The black arrow represents cytoplasmic vacuolization, nuclear pyknosis and poorly defined nucleoli. Statistical graph of nissl body in the mPFC. n = 3 per group. K Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11, n = 3 per group. L Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Representative immunofluorescence images of ROS (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ROS, n = 3 per group. N Electron microscope of mitochondrial morphology in the P24 mPFC. The second row represents a partial enlargement of the first row, respectively. Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: GPX4 (1:2000; Cat# A11243; Abclonal), FTH1 (1:5000; Cat# A19544; Abclonal), ACSL4 (1:1000; Cat#38493T; Cell Signaling Technology), xCT/SLC7A11 (1:1000; Cat# 28001T; Cell Signaling Technology), and β-actin (1:50000; Cat# 66009-1-Ig; Proteintech).

Techniques: Concentration Assay, H&E Stain, Staining, Immunofluorescence, Fluorescence, Microscopy

Journal: Molecular Biomedicine

Article Title: Activating Cannabinoid receptor 2 alleviates iron overload-induced liver damage via dual modulation of STAT3/hepcidin and Nrf2/FPN1 pathways

doi: 10.1186/s43556-026-00541-1

Figure Lengend Snippet: Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c Serum ferritin levels determined using ELISA ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots

Article Snippet: The mouse serum Ferritin elisa kit (41-FERMS-E01) was purchased from ALPCO (Beijing, China).

Techniques: Activation Assay, Staining, Ferrozine Assay, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Cell Reports Medicine

Article Title: Multi-omics profiling unveils biological and clinical insights into pulmonary sarcomatoid carcinoma

doi: 10.1016/j.xcrm.2026.102887

Figure Lengend Snippet: Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of SLC3A2 and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: Cells were lysed with 1% Triton X-100 buffer (Beyotime, ST1723) and then boiled in 5× loading buffer for 10 min. Each sample was electrophoresed on 10% PAGE gels, then transferred to PVDF membranes and immunoblotted with the antibodies targeting SLC3A2 (Abclonal, A24375), FTL (Abclonal, A11241), GPX4 (Abclonal, A1933) and β-Actin (Abclonal, AC004).

Techniques: Western Blot, Knockdown, CCK-8 Assay, Cell Culture, Staining, Software, Concentration Assay, Acid Assay, Negative Control