fcγriii  (Sino Biological)


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  • 93
    Name:
    MSP3 merozoite surface protein 3 cDNA ORF Clone in Cloning Vector P falciparum
    Description:
    Full length Clone DNA of Plasmodium falciparum strain 3D7 merozoite surface protein 3
    Catalog Number:
    VG40302-G
    Price:
    95.0
    Category:
    cDNA Clone
    Size:
    1Unit
    Molecule Name:
    PF3D7_1354
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    Structured Review

    Sino Biological fcγriii
    Full length Clone DNA of Plasmodium falciparum strain 3D7 merozoite surface protein 3
    https://www.bioz.com/result/fcγriii/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fcγriii - by Bioz Stars, 2021-05
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Sero-diagnostic potential of Plasmodium falciparum recombinant merozoite surface protein (MSP)-3 expressed in silkworm.
    Article Snippet: Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries. .. Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries. .. Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries.

    Article Title: Glycan variants of a respiratory syncytial virus antibody with enhanced effector function and in vivo efficacy
    Article Snippet: Fast flow rates and controls, including a flow cell with no gF and immobilized gF with the flow of buffer only, were used to ensure against acquiring mass transfer-limited data. .. Recombinant human Fcγ receptors (Sino Biological) were captured on the surface of NTA chips (GE Healthcare), with a target capture level of 1000 RUs. ..

    Article Title: Antibody production using a ciliate generates unusual antibody glycoforms displaying enhanced cell-killing activity
    Article Snippet: Purification of anti-CD20 antibody expressed by T. thermophilaCell-free supernatants harvested from fermentation processes were applied to a Protein A agarose column (mABSelectSuRe, GE Healthcare) equilibrated with 10 mM phosphate buffer containing 140 mM sodium chloride (pH 7.2). .. Afterwards, recombinant histidine-tagged human ectodomains of FcγRIIIA variants (Sino Biological) were immobilized onto the surface using a previously published protocol. .. In antibody-containing fractions, a buffer exchange into stabilizing buffer (9 mg/L NaCl, 7.35 mg/L sodium citrate dihydrate, 0.7 mg/L polysorbate 80, pH 6.8) was performed using Vivaspin 20 columns (Sartorius).

    Article Title: Enhanced potency of a fucose-free monoclonal antibody being developed as an Ebola virus immunoprotectant
    Article Snippet: .. Recombinant human FcγRI and FcγRIII (Sino Biological, China) were immobilized onto the surface of CM5 chips (GE Healthcare) using an amine-coupling kit with a target capture level of 1,000 RUs. .. Each mAb (diluted in HBS-EP+ buffer; GE Healthcare) was then flowed over the chip at five different concentrations (with the highest concentration having an R max between 30 and 80 RUs), and kinetic analyses using BIAEvaluation software were performed (1:1 fit).

    Polymerase Chain Reaction:

    Article Title: Sero-diagnostic potential of Plasmodium falciparum recombinant merozoite surface protein (MSP)-3 expressed in silkworm.
    Article Snippet: Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries. .. Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries. .. Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries.

    Amplification:

    Article Title: Sero-diagnostic potential of Plasmodium falciparum recombinant merozoite surface protein (MSP)-3 expressed in silkworm.
    Article Snippet: Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries. .. Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries. .. Plasmodium falciparum is a blood protozoan parasite, transmitted by Anopheles mosquitoes vectors, that can cause morbidity and even leads to mortality in tropical countries.

    Binding Assay:

    Article Title: Anti-Hemagglutinin Antibody Derived Lead Peptides for Inhibitors of Influenza Virus Binding
    Article Snippet: .. As it is not possible to calculate reliable binding constants from the multivalent binding, the inverse experiment using surface immobilized HA from Aichi H3N2 was performed. .. From steady-state affinity calculation for PeB a binding constant K D of 56.8 μM (R max = 35.6 RU; chi 2 = 0.088), was obtained ( ).

    Construct:

    Article Title: Decoding the Human Immunoglobulin G-Glycan Repertoire Reveals a Spectrum of Fc-Receptor- and Complement-Mediated-Effector Activities
    Article Snippet: .. Human FcγR ConstructsHuman FcγR constructs [FcγRIa (HIS tag), FcγRIIa (131His, Biotinylated, and 131Arg, Biotinylated), FcγRIIb (Biotinylated), FcγRIIIa (158Phe, Biotinylated, and 158Val, Biotinylated) and FcγRIIIb (NA2, HIS tag)] for surface plasmon resonance (SPR) analysis were obtained from Sino biological (Beijing, China). .. To further include all human FcγRs, a fusion Fc–FcγR construct composed of the extracellular domain of the FcγRIIIb in both allotypes followed by a Fc domain was created.

    Article Title: FcγR Binding and ADCC Activity of Human IgG Allotypes
    Article Snippet: A binary gradient of water and 95% acetonitrile (LC-MS grade Biosolve, Valkenswaard, Netherlands) with 0.1% formic acid each consisted of the following steps: 0–5 min 1% B, linear gradient to 27% B 5–20 min, washing at 70% B 21–23 min, and re-equilibration at 1% B 24–42 min. LC-MS data was automatically (pre-)processed with LaCyTools version 1.1.0 alpha build 190207a as previously described, albeit with an extraction window of 65 mTh and without the need to align or calibrate ( , ). .. Human FcγR Constructs and Control AntibodiesHuman FcγR constructs FcγRIa (his tag, 10256-H08H-100), FcγRIIa (131His, biotinylated, 10374-H27H1-B-50 and 131Arg, biotinylated, 10374-H27H-B-50), FcγRIIb (biotinylated, 10259-H27H-B-50), and FcγRIIIa (158Phe, biotinylated, 10389-H27H-B-50, and 158Val, biotinylated, 10389-H27H1-B-50) for surface plasmon resonance (SPR) analysis were obtained from Sino Biological (Beijing, China). .. We used Fc-FcγRIIIB fusion proteins to determine binding affinities to two polymorphic variants of FcγRIIIB (NA1 and NA2), as described previously ( ).

    SPR Assay:

    Article Title: Decoding the Human Immunoglobulin G-Glycan Repertoire Reveals a Spectrum of Fc-Receptor- and Complement-Mediated-Effector Activities
    Article Snippet: .. Human FcγR ConstructsHuman FcγR constructs [FcγRIa (HIS tag), FcγRIIa (131His, Biotinylated, and 131Arg, Biotinylated), FcγRIIb (Biotinylated), FcγRIIIa (158Phe, Biotinylated, and 158Val, Biotinylated) and FcγRIIIb (NA2, HIS tag)] for surface plasmon resonance (SPR) analysis were obtained from Sino biological (Beijing, China). .. To further include all human FcγRs, a fusion Fc–FcγR construct composed of the extracellular domain of the FcγRIIIb in both allotypes followed by a Fc domain was created.

    Article Title: FcγR Binding and ADCC Activity of Human IgG Allotypes
    Article Snippet: A binary gradient of water and 95% acetonitrile (LC-MS grade Biosolve, Valkenswaard, Netherlands) with 0.1% formic acid each consisted of the following steps: 0–5 min 1% B, linear gradient to 27% B 5–20 min, washing at 70% B 21–23 min, and re-equilibration at 1% B 24–42 min. LC-MS data was automatically (pre-)processed with LaCyTools version 1.1.0 alpha build 190207a as previously described, albeit with an extraction window of 65 mTh and without the need to align or calibrate ( , ). .. Human FcγR Constructs and Control AntibodiesHuman FcγR constructs FcγRIa (his tag, 10256-H08H-100), FcγRIIa (131His, biotinylated, 10374-H27H1-B-50 and 131Arg, biotinylated, 10374-H27H-B-50), FcγRIIb (biotinylated, 10259-H27H-B-50), and FcγRIIIa (158Phe, biotinylated, 10389-H27H-B-50, and 158Val, biotinylated, 10389-H27H1-B-50) for surface plasmon resonance (SPR) analysis were obtained from Sino Biological (Beijing, China). .. We used Fc-FcγRIIIB fusion proteins to determine binding affinities to two polymorphic variants of FcγRIIIB (NA1 and NA2), as described previously ( ).

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  • 92
    Sino Biological preparation human fcγriii protein
    (A) FPOP kinetic plots of representative residues of <t>FcγRIII.</t> The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.
    Preparation Human Fcγriii Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparation human fcγriii protein/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    preparation human fcγriii protein - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    92
    Sino Biological fcγriii
    Characterization of the isolated FcγRIV-specific VHH. (A) The predicted amino acid residue sequence of FcγRIV-VHH-7m, isolated after panning on recombinant mouse FcγRIV protein of a VHH library derived from a llama immunized with immature mouse dendritic cells. Above the sequences the Kabat numbering is indicated. CDR1, −2, and −3 are boxed. (B) Flow cytometric analysis showing binding of FcgRIV-VHH-7m and M2e-VHH-23m to HEK 293T cells transiently transfected with expression vectors coding for mouse FcγRI, FγRIIb, <t>FcγRIII,</t> and FcγRIV along with the common γ-chain for the activating FcγRs and a GFP expression plasmid. The lower graph depicts binding to Mf4/4 cells. The cells were incubated with fourfold serial dilution of Alexa Fluor™ 647 labeled FcγRIV-VHH-7m or M2e-VHH-23m, starting at a concentration of 0.2 μM, binding of the VHHs to the GFP positive cells was analyzed. Data points represent averages of triplicates and error bars standard deviations.
    Fcγriii, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fcγriii/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fcγriii - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    (A) FPOP kinetic plots of representative residues of FcγRIII. The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.

    Journal: Biochemistry

    Article Title: Recognition of Human IgG1 by Fcγ Receptors: Structural Insights from Hydrogen-Deuterium Exchange and Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry

    doi: 10.1021/acs.biochem.8b01048

    Figure Lengend Snippet: (A) FPOP kinetic plots of representative residues of FcγRIII. The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.

    Article Snippet: Materials and sample preparation Human FcγRIII protein was purchased from Sino Biological Inc. (Beijing, China).

    Techniques: Modification

    (A) HDX kinetic plots of representative peptides of FcγRIII in the unbound (black) and bound (red) states. The peptide, its charge state, and its amino acid sequence are labeled on each panel. (B) Differential deuterium uptake percentage of FcγRIII mapped onto the crystal structure. The color scale representing the difference between bound and unbound is shown in the bottom of the panel. Mirrored views are provided for clarity.

    Journal: Biochemistry

    Article Title: Recognition of Human IgG1 by Fcγ Receptors: Structural Insights from Hydrogen-Deuterium Exchange and Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry

    doi: 10.1021/acs.biochem.8b01048

    Figure Lengend Snippet: (A) HDX kinetic plots of representative peptides of FcγRIII in the unbound (black) and bound (red) states. The peptide, its charge state, and its amino acid sequence are labeled on each panel. (B) Differential deuterium uptake percentage of FcγRIII mapped onto the crystal structure. The color scale representing the difference between bound and unbound is shown in the bottom of the panel. Mirrored views are provided for clarity.

    Article Snippet: Materials and sample preparation Human FcγRIII protein was purchased from Sino Biological Inc. (Beijing, China).

    Techniques: Sequencing, Labeling

    Characterization of the isolated FcγRIV-specific VHH. (A) The predicted amino acid residue sequence of FcγRIV-VHH-7m, isolated after panning on recombinant mouse FcγRIV protein of a VHH library derived from a llama immunized with immature mouse dendritic cells. Above the sequences the Kabat numbering is indicated. CDR1, −2, and −3 are boxed. (B) Flow cytometric analysis showing binding of FcgRIV-VHH-7m and M2e-VHH-23m to HEK 293T cells transiently transfected with expression vectors coding for mouse FcγRI, FγRIIb, FcγRIII, and FcγRIV along with the common γ-chain for the activating FcγRs and a GFP expression plasmid. The lower graph depicts binding to Mf4/4 cells. The cells were incubated with fourfold serial dilution of Alexa Fluor™ 647 labeled FcγRIV-VHH-7m or M2e-VHH-23m, starting at a concentration of 0.2 μM, binding of the VHHs to the GFP positive cells was analyzed. Data points represent averages of triplicates and error bars standard deviations.

    Journal: Frontiers in Immunology

    Article Title: Selective Engagement of FcγRIV by a M2e-Specific Single Domain Antibody Construct Protects Against Influenza A Virus Infection

    doi: 10.3389/fimmu.2019.02920

    Figure Lengend Snippet: Characterization of the isolated FcγRIV-specific VHH. (A) The predicted amino acid residue sequence of FcγRIV-VHH-7m, isolated after panning on recombinant mouse FcγRIV protein of a VHH library derived from a llama immunized with immature mouse dendritic cells. Above the sequences the Kabat numbering is indicated. CDR1, −2, and −3 are boxed. (B) Flow cytometric analysis showing binding of FcgRIV-VHH-7m and M2e-VHH-23m to HEK 293T cells transiently transfected with expression vectors coding for mouse FcγRI, FγRIIb, FcγRIII, and FcγRIV along with the common γ-chain for the activating FcγRs and a GFP expression plasmid. The lower graph depicts binding to Mf4/4 cells. The cells were incubated with fourfold serial dilution of Alexa Fluor™ 647 labeled FcγRIV-VHH-7m or M2e-VHH-23m, starting at a concentration of 0.2 μM, binding of the VHHs to the GFP positive cells was analyzed. Data points represent averages of triplicates and error bars standard deviations.

    Article Snippet: VHH Binding to FcγRs Expressing Cells Human Embryonic Kidney (HEK) 293T cells were transiently transfected with full length mouse FcγRI (MG50086-CF), FcγRIIb (MG50030-CY, SinoBiological Inc.), FcγRIII (MG50326, SinoBiological Inc.) or FcγRIV (MG50036-CF, SinoBiological Inc.) expression constructs along with the common γ-chain for the activating FcγRs (MG50935-CF) by polyethylenimine (PEI)-based transfection.

    Techniques: Isolation, Sequencing, Recombinant, Derivative Assay, Flow Cytometry, Binding Assay, Transfection, Expressing, Plasmid Preparation, Incubation, Serial Dilution, Labeling, Concentration Assay