fc oxyburst immune complex  (Thermo Fisher)


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    Thermo Fisher fc oxyburst immune complex
    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc <t>oxyburst</t> assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
    Fc Oxyburst Immune Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fc oxyburst immune complex/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fc oxyburst immune complex - by Bioz Stars, 2020-07
    92/100 stars

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    Images

    1) Product Images from "Genetic Susceptibility to Experimental Autoimmune Glomerulonephritis in the Wistar Kyoto Rat"

    Article Title: Genetic Susceptibility to Experimental Autoimmune Glomerulonephritis in the Wistar Kyoto Rat

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2012.01.029

    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc oxyburst assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
    Figure Legend Snippet: Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc oxyburst assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P

    Techniques Used: Activation Assay, Phagocytosis Assay, Expressing

    Related Articles

    Purification:

    Article Title: The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fc?IIA receptor-induced phagocytosis
    Article Snippet: .. NADPH oxidase activity was also measured using zymosan opsonized with Fc OxyBURST Green (Invitrogen), where dichlorodihydrofluorescein (H2 DCF) is covalently linked to BSA and then complexed with purified rabbit polyclonal anti-BSA antibodies ( ). ..

    Modification:

    Article Title: A Fluorescently Tagged C-Terminal Fragment of p47phox Detects NADPH Oxidase Dynamics during Phagocytosis
    Article Snippet: .. Intracellular O2 − production in cells stimulated with Fc OxyBURST Green (Molecular Probes) was measured in a luminol-based chemiluminescence assay modified from a synchronized phagocytosis assay ( ; ). .. Briefly, 5 × 105 neutrophil-differentiated PLB-985-mCherry-p40PX cells in 200 μl PBSG were added to wells of a 96-well plate, and incubated for 5 min on ice, and then 25 μl cold Fc OxyBURST Green particles was added to the cells.

    other:

    Article Title: A Fluorescently Tagged C-Terminal Fragment of p47phox Detects NADPH Oxidase Dynamics during Phagocytosis
    Article Snippet: Fc OxyBURST Green (F2902) was purchased from Invitrogen.

    Chemiluminescence Immunoassay:

    Article Title: A Fluorescently Tagged C-Terminal Fragment of p47phox Detects NADPH Oxidase Dynamics during Phagocytosis
    Article Snippet: .. Intracellular O2 − production in cells stimulated with Fc OxyBURST Green (Molecular Probes) was measured in a luminol-based chemiluminescence assay modified from a synchronized phagocytosis assay ( ; ). .. Briefly, 5 × 105 neutrophil-differentiated PLB-985-mCherry-p40PX cells in 200 μl PBSG were added to wells of a 96-well plate, and incubated for 5 min on ice, and then 25 μl cold Fc OxyBURST Green particles was added to the cells.

    Phagocytosis Assay:

    Article Title: A Fluorescently Tagged C-Terminal Fragment of p47phox Detects NADPH Oxidase Dynamics during Phagocytosis
    Article Snippet: .. Intracellular O2 − production in cells stimulated with Fc OxyBURST Green (Molecular Probes) was measured in a luminol-based chemiluminescence assay modified from a synchronized phagocytosis assay ( ; ). .. Briefly, 5 × 105 neutrophil-differentiated PLB-985-mCherry-p40PX cells in 200 μl PBSG were added to wells of a 96-well plate, and incubated for 5 min on ice, and then 25 μl cold Fc OxyBURST Green particles was added to the cells.

    Activity Assay:

    Article Title: The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fc?IIA receptor-induced phagocytosis
    Article Snippet: .. NADPH oxidase activity was also measured using zymosan opsonized with Fc OxyBURST Green (Invitrogen), where dichlorodihydrofluorescein (H2 DCF) is covalently linked to BSA and then complexed with purified rabbit polyclonal anti-BSA antibodies ( ). ..

    Article Title: HIF-1? expression regulates the bactericidal capacity of phagocytes
    Article Snippet: .. Oxidative burst activity was measured by using the Fc OxyBURST Green assay reagent (Invitrogen Corp.) according to the manufacturer’s instructions. ..

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    Thermo Fisher fc oxyburst assay reagents
    Fc Oxyburst Assay Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fc oxyburst assay reagents/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fc oxyburst assay reagents - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Thermo Fisher fc oxyburst green reagent
    Inhibition of glucose catabolism reduces phagocytic and bactericidal activities in liver phagocytes from pioglitazone-treated mice. (a) Intracellular killing assay for liver MNCs in control and pioglitazone-treated elderly mice. Liver MNCs were pre-incubated for 2 h with DMSO, 2DG, or oligomycin A, and cultured with viable E. coli (1 × 10 7 bacteria per 5 × 10 5 cells) at 37°C for 45 min, before eliminating free bacteria by centrifugation and re-suspension in gentamicin solution (100 µg/ml). (b, c) Phagocytic activity of liver recruited Mφ in which glycolysis or OXPHOS were metabolically inhibited. (b) Liver MNCs from control and pioglitazone-treated elderly mice were preincubated for 2 h with 2DG, oligomycin A, or DMSO. They were either chilled on ice or incubated at 37°C with FITC- E. coli for 1 h, and positive recruited Mφ were determined by flow cytometry. The results were determined by subtracting the percentages of positive populations for on ice from those for incubation at 37°C. (c) Liver MNCs from control and pioglitazone-treated elderly mice were incubated with pHrodo® E. coli for 1 h after metabolic inhibition, and then positive recruited Mφ were determined. (d) Effect of metabolic inhibition on intracellular CD206 expression in liver recruited Mφ from control and pioglitazone-treated elderly mice. After incubating liver MNCs from the mice for 2 h with 2DG, oligomycin A, or DMSO, CD206 expression in recruited Mφ was analyzed. (e) Effect of metabolic inhibition on ROS produced by liver recruited Mφ in control and pioglitazone-treated elderly mice. Liver MNCs from the mice were preincubated for 2 h with 2DG, oligomycin A, or DMSO. They were cultured with or without heat-killed E. coli for 1 h, and then incubated with Fc <t>OxyBURST®</t> reagent, followed by cell surface staining. Liver recruited Mφ were analyzed for Fc OxyBURST® staining by flow cytometry. Data are means ± SEM from four samples in each group. * P
    Fc Oxyburst Green Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fc oxyburst green reagent/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fc oxyburst green reagent - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of glucose catabolism reduces phagocytic and bactericidal activities in liver phagocytes from pioglitazone-treated mice. (a) Intracellular killing assay for liver MNCs in control and pioglitazone-treated elderly mice. Liver MNCs were pre-incubated for 2 h with DMSO, 2DG, or oligomycin A, and cultured with viable E. coli (1 × 10 7 bacteria per 5 × 10 5 cells) at 37°C for 45 min, before eliminating free bacteria by centrifugation and re-suspension in gentamicin solution (100 µg/ml). (b, c) Phagocytic activity of liver recruited Mφ in which glycolysis or OXPHOS were metabolically inhibited. (b) Liver MNCs from control and pioglitazone-treated elderly mice were preincubated for 2 h with 2DG, oligomycin A, or DMSO. They were either chilled on ice or incubated at 37°C with FITC- E. coli for 1 h, and positive recruited Mφ were determined by flow cytometry. The results were determined by subtracting the percentages of positive populations for on ice from those for incubation at 37°C. (c) Liver MNCs from control and pioglitazone-treated elderly mice were incubated with pHrodo® E. coli for 1 h after metabolic inhibition, and then positive recruited Mφ were determined. (d) Effect of metabolic inhibition on intracellular CD206 expression in liver recruited Mφ from control and pioglitazone-treated elderly mice. After incubating liver MNCs from the mice for 2 h with 2DG, oligomycin A, or DMSO, CD206 expression in recruited Mφ was analyzed. (e) Effect of metabolic inhibition on ROS produced by liver recruited Mφ in control and pioglitazone-treated elderly mice. Liver MNCs from the mice were preincubated for 2 h with 2DG, oligomycin A, or DMSO. They were cultured with or without heat-killed E. coli for 1 h, and then incubated with Fc OxyBURST® reagent, followed by cell surface staining. Liver recruited Mφ were analyzed for Fc OxyBURST® staining by flow cytometry. Data are means ± SEM from four samples in each group. * P

    Journal: Innate Immunity

    Article Title: Pioglitazone improves phagocytic activity of liver recruited macrophages in elderly mice possibly by promoting glucose catabolism

    doi: 10.1177/1753425919849620

    Figure Lengend Snippet: Inhibition of glucose catabolism reduces phagocytic and bactericidal activities in liver phagocytes from pioglitazone-treated mice. (a) Intracellular killing assay for liver MNCs in control and pioglitazone-treated elderly mice. Liver MNCs were pre-incubated for 2 h with DMSO, 2DG, or oligomycin A, and cultured with viable E. coli (1 × 10 7 bacteria per 5 × 10 5 cells) at 37°C for 45 min, before eliminating free bacteria by centrifugation and re-suspension in gentamicin solution (100 µg/ml). (b, c) Phagocytic activity of liver recruited Mφ in which glycolysis or OXPHOS were metabolically inhibited. (b) Liver MNCs from control and pioglitazone-treated elderly mice were preincubated for 2 h with 2DG, oligomycin A, or DMSO. They were either chilled on ice or incubated at 37°C with FITC- E. coli for 1 h, and positive recruited Mφ were determined by flow cytometry. The results were determined by subtracting the percentages of positive populations for on ice from those for incubation at 37°C. (c) Liver MNCs from control and pioglitazone-treated elderly mice were incubated with pHrodo® E. coli for 1 h after metabolic inhibition, and then positive recruited Mφ were determined. (d) Effect of metabolic inhibition on intracellular CD206 expression in liver recruited Mφ from control and pioglitazone-treated elderly mice. After incubating liver MNCs from the mice for 2 h with 2DG, oligomycin A, or DMSO, CD206 expression in recruited Mφ was analyzed. (e) Effect of metabolic inhibition on ROS produced by liver recruited Mφ in control and pioglitazone-treated elderly mice. Liver MNCs from the mice were preincubated for 2 h with 2DG, oligomycin A, or DMSO. They were cultured with or without heat-killed E. coli for 1 h, and then incubated with Fc OxyBURST® reagent, followed by cell surface staining. Liver recruited Mφ were analyzed for Fc OxyBURST® staining by flow cytometry. Data are means ± SEM from four samples in each group. * P

    Article Snippet: Determination of ROS production We used Fc OXYBURST® green reagent (Thermo Fisher Scientific K.K.) in the determination of ROS production from liver MNCs before staining their surface Ags.

    Techniques: Inhibition, Mouse Assay, Incubation, Cell Culture, Centrifugation, Activity Assay, Metabolic Labelling, Flow Cytometry, Expressing, Produced, Staining

    Pioglitazone increases phagocytic activity of liver recruited Mφ. (a) Phagocytic activity of liver recruited Mφ and neutrophils in elderly mice for control, pioglitazone-treatment, and GW9662 plus pioglitazone treatment. Liver MNCs were obtained from the control mice, those with pioglitazone treatment and those receiving GW9662 plus pioglitazone treatment, and then cultured with pHrodo® E. coli for 1 h. We determined pHrodo® E. coli -positive recruited Mφ and neutrophils by flow cytometry. (b) Engulfment of FITC- E. coli by liver recruited Mφ and neutrophils in control and pioglitazone-treated elderly mice. The results were determined by subtracting the percentages of positive populations for on ice from those for incubation at 37°C (Supplemental Figure 5). (c) CD206 expression after pioglitazone treatment. Liver MNCs were obtained from elderly mice 3 h after pioglitazone or vehicle treatment, and then incubated at 37°C for 2 h to determine intracellular CD206 expression in the recruited Mφ and neutrophils. Data are shown as MFI, and isotype controls are shown on the left. (d and e) ROS production by liver recruited Mφ and neutrophils. Liver MNCs were harvested from elderly mice 3 h after administering pioglitazone. Non-stimulated (left panels), PMA-stimulated (middle panels), or heat-killed E. coli -stimulated (right panels) MNCs were incubated with Fc OxyBURST® reagent, followed by cell surface staining. Recruited Mφ and neutrophils were analyzed for Fc OxyBURST® staining by flow cytometry. (d) Representative histograms for Fc OxyBURST® staining in recruited Mφ (upper panels) and neutrophils (lower panels). The dotted curves are for control mice, and the solid curves for pioglitazone-treated mice. (e) MFI for Fc OxyBURST® staining in recruited Mφ (upper graph) and neutrophils (lower graph) cultured with heat-killed E. coli . Data are means ± SEM from 4 mice in each group. * P

    Journal: Innate Immunity

    Article Title: Pioglitazone improves phagocytic activity of liver recruited macrophages in elderly mice possibly by promoting glucose catabolism

    doi: 10.1177/1753425919849620

    Figure Lengend Snippet: Pioglitazone increases phagocytic activity of liver recruited Mφ. (a) Phagocytic activity of liver recruited Mφ and neutrophils in elderly mice for control, pioglitazone-treatment, and GW9662 plus pioglitazone treatment. Liver MNCs were obtained from the control mice, those with pioglitazone treatment and those receiving GW9662 plus pioglitazone treatment, and then cultured with pHrodo® E. coli for 1 h. We determined pHrodo® E. coli -positive recruited Mφ and neutrophils by flow cytometry. (b) Engulfment of FITC- E. coli by liver recruited Mφ and neutrophils in control and pioglitazone-treated elderly mice. The results were determined by subtracting the percentages of positive populations for on ice from those for incubation at 37°C (Supplemental Figure 5). (c) CD206 expression after pioglitazone treatment. Liver MNCs were obtained from elderly mice 3 h after pioglitazone or vehicle treatment, and then incubated at 37°C for 2 h to determine intracellular CD206 expression in the recruited Mφ and neutrophils. Data are shown as MFI, and isotype controls are shown on the left. (d and e) ROS production by liver recruited Mφ and neutrophils. Liver MNCs were harvested from elderly mice 3 h after administering pioglitazone. Non-stimulated (left panels), PMA-stimulated (middle panels), or heat-killed E. coli -stimulated (right panels) MNCs were incubated with Fc OxyBURST® reagent, followed by cell surface staining. Recruited Mφ and neutrophils were analyzed for Fc OxyBURST® staining by flow cytometry. (d) Representative histograms for Fc OxyBURST® staining in recruited Mφ (upper panels) and neutrophils (lower panels). The dotted curves are for control mice, and the solid curves for pioglitazone-treated mice. (e) MFI for Fc OxyBURST® staining in recruited Mφ (upper graph) and neutrophils (lower graph) cultured with heat-killed E. coli . Data are means ± SEM from 4 mice in each group. * P

    Article Snippet: Determination of ROS production We used Fc OXYBURST® green reagent (Thermo Fisher Scientific K.K.) in the determination of ROS production from liver MNCs before staining their surface Ags.

    Techniques: Activity Assay, Mouse Assay, Cell Culture, Flow Cytometry, Incubation, Expressing, Staining