fbs  (GE Healthcare)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    Penicillin Streptomycin solution
    Description:

    Catalog Number:
    sv30010
    Price:
    18.36 USD
    Size:
    100 mL
    Buy from Supplier


    Structured Review

    GE Healthcare fbs

    https://www.bioz.com/result/fbs/product/GE Healthcare
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fbs - by Bioz Stars, 2021-03
    97/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: VDAC3 regulates centriole assembly by targeting Mps1 to centrosomes
    Article Snippet: .. HEK293, HeLa S3, U2OS and NIH3T3 cells were cultured in DMEM and hTERT-RPE1 cells in DME/F-12 (1:1) (Hyclone) supplemented with 10% FBS (Atlanta Biologicals), 100 U/ml penicillin G and 100 μg/ml streptomycin (Hyclone) in the presence of 5% CO2. .. The expression of GFP-Mps1∆12/13 in HeLa GFP-Mps1∆12/13 cells that were maintained of 500 μg/ml G418 (Sigma) was induced by doxycycline (dox; Sigma) at 1 μg/ml.

    Article Title: Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway
    Article Snippet: .. Cell Culture The human hepatocellular carcinoma cell line HepG2 cells, human lung adenocarcinoma cell line A549 cells, human gastric adenocarcinoma cell line SGC7901 cells, and human breast carcinoma cell line MCF-7 cells were bought from the cell bank of Chinese Academy of Sciences and cultured in Dulbecco’s modified Eagle medium (DMEM) (Hyclone, South Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin solution (Hyclone, South Logan, UT, USA). ..

    Article Title: lncRNA PTAR promotes NSCLC cell proliferation, migration and invasion by sponging microRNA-101
    Article Snippet: .. Cell culture and transfection All cells were cultured in Dulbecco's Modified Eagle's medium (Hyclone; GE Healthcare) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare) and 100 U/ml penicillin/streptomycin (Hyclone; GE Healthcare). .. Phosphate-buffered saline (PBS; Hyclone; GE Healthcare) solution was used for washing cells before dissociation and diluting cells for counting.

    Article Title: C18H17NO6 Inhibits Invasion and Migration of Human MNNG Osteosarcoma Cells via the PI3K/AKT Signaling Pathway
    Article Snippet: .. MNNG cells were cultured at 37°C in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin-streptomycin (HyClone, USA) in a cell incubator containing 5% CO2 . .. When the cells grew to 80–90% confluence, digestion was terminated with 0.25% trypsin (HyClone, USA) after 2 min. After centrifugation at 600 rpm for 5 min, the cells were collected and subcultured according to experimental protocols.

    Modification:

    Article Title: Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway
    Article Snippet: .. Cell Culture The human hepatocellular carcinoma cell line HepG2 cells, human lung adenocarcinoma cell line A549 cells, human gastric adenocarcinoma cell line SGC7901 cells, and human breast carcinoma cell line MCF-7 cells were bought from the cell bank of Chinese Academy of Sciences and cultured in Dulbecco’s modified Eagle medium (DMEM) (Hyclone, South Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin solution (Hyclone, South Logan, UT, USA). ..

    Article Title: lncRNA PTAR promotes NSCLC cell proliferation, migration and invasion by sponging microRNA-101
    Article Snippet: .. Cell culture and transfection All cells were cultured in Dulbecco's Modified Eagle's medium (Hyclone; GE Healthcare) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare) and 100 U/ml penicillin/streptomycin (Hyclone; GE Healthcare). .. Phosphate-buffered saline (PBS; Hyclone; GE Healthcare) solution was used for washing cells before dissociation and diluting cells for counting.

    Article Title: C18H17NO6 Inhibits Invasion and Migration of Human MNNG Osteosarcoma Cells via the PI3K/AKT Signaling Pathway
    Article Snippet: .. MNNG cells were cultured at 37°C in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin-streptomycin (HyClone, USA) in a cell incubator containing 5% CO2 . .. When the cells grew to 80–90% confluence, digestion was terminated with 0.25% trypsin (HyClone, USA) after 2 min. After centrifugation at 600 rpm for 5 min, the cells were collected and subcultured according to experimental protocols.

    other:

    Article Title: Endogenous n-3 Polyunsaturated Fatty Acids Are Beneficial to Dampen CD8+ T Cell-Mediated Inflammatory Response upon the Viral Infection in Mice
    Article Snippet: Reagents and Antibodies For splenocytes culture, RPMI medium supplemented with penicillin/streptomycin (Wellgene, Seoul, Korea) and 10% fetal bovine serum (Hyclone, Pittsburgh, PA, USA) were used.

    Transfection:

    Article Title: lncRNA PTAR promotes NSCLC cell proliferation, migration and invasion by sponging microRNA-101
    Article Snippet: .. Cell culture and transfection All cells were cultured in Dulbecco's Modified Eagle's medium (Hyclone; GE Healthcare) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare) and 100 U/ml penicillin/streptomycin (Hyclone; GE Healthcare). .. Phosphate-buffered saline (PBS; Hyclone; GE Healthcare) solution was used for washing cells before dissociation and diluting cells for counting.

    Size-exclusion Chromatography:

    Article Title: Molecular and structural analysis of mosaic variants of penicillin-binding protein 2 conferring decreased susceptibility to expanded-spectrum cephalosporins in Neisseria gonorrhoeae: role of epistatic mutations †
    Article Snippet: The reaction of β-lactam antibiotics with PBP 2 is denoted by the following equation: E + S ↔ K s E • S → k 2 E − S ′ → k 3 E + P , where E•S is the non-covalent enzyme-antibiotic complex, E-S’ is the acyl-enzyme complex, and P the hydrolyzed antibiotic. k2 /Ks constants, which are a direct measure of the ability of an antibiotic to inhibit a PBP , were calculated from first order rates of acylation of purified, soluble PBP 2 variants by [14 C]penicillin G (Moravek, Brea, CA) as previously described ( , ). .. Graphs of PBP 2-[14 C]penicillin G complex formation versus time were obtained by incubating 27 μg of protein with 1.0 μM [14 C]penicillin G, and aliquots of ~4 μg were removed at 15 sec intervals, precipitated with 5% trichloroacetic acid, filtered over Whatman GC-A filters, and the filters were submitted to scintillation counting. .. The concentration of [14 C]penicillin G was increased to 25 and 50 μM for determination of k2 /KS values with PBP 235/02 and PBP 235/02 -A501V, respectively.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    GE Healthcare hi fbs
    Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI <t>FBS</t> CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show <t>RT-PCR</t> products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p
    Hi Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hi fbs/product/GE Healthcare
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hi fbs - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    86
    GE Healthcare fbs
    Knockdown of Sp1 Decreases the Myogenic Differentiation of Hypoxic-EBs (A) Generation of Sp1-knockdown stable cells. C57 ESCs were transfected with 1 μg shMock or shSp1 for 24 h, and further selected by puromycin treatment. MyoD protein was remarkably decreased in all four Sp1-knockdown hypoxic-ES clones compared to that in all four control clones transfected with shMock. (B) Stable knockdown cells were formed as EBs by the hanging drop method, cultured under normoxic or hypoxic conditions, and plated onto a gelatin-coated plate in <t>DMEM/10%</t> <t>FBS</t> for 1 day. The culture medium was replaced with SkIM and further incubated for up to 20 days. (C) Muscle regulatory factors (MyoD, myogenin) were increased in Hyp-shMock cells compared to those in Nor-shMock cells, and were significantly decreased to a greater extent in Hyp-shSp1 cells compared to expression in Hyp-shMock cells (n = 6); *p
    Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

    Journal: Biological Research

    Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

    doi: 10.1186/s40659-017-0148-1

    Figure Lengend Snippet: Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

    Article Snippet: Expression of RGS2 and RGS4 was examined by RT-PCR in hMSCs cultured in 8 different media treatments, including Hyclone CM, HI-FBS CM, Hyclone CM based DEX media (Hyclone 1.0 µM DEX), HI-FBS CM based DEX media (HI-FBS 1.0 µM DEX), Hyclone CM based AIM media with 1.0 µM DEX (Hyclone 1.0 µM DEX AIM), FBS CM based AIM media with 1 µM DEX (FBS 1.0 µM DEX AIM), HI-FBS CM based AIM media with 1.0 µM DEX (HI-FBS 1.0 µM DEX AIM), and Hyclone CM based OIM media with 1.0 µM DEX (Hyclone 1.0 µM DEX OIM).

    Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction

    Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

    Journal: Biological Research

    Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

    doi: 10.1186/s40659-017-0148-1

    Figure Lengend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

    Article Snippet: Expression of RGS2 and RGS4 was examined by RT-PCR in hMSCs cultured in 8 different media treatments, including Hyclone CM, HI-FBS CM, Hyclone CM based DEX media (Hyclone 1.0 µM DEX), HI-FBS CM based DEX media (HI-FBS 1.0 µM DEX), Hyclone CM based AIM media with 1.0 µM DEX (Hyclone 1.0 µM DEX AIM), FBS CM based AIM media with 1 µM DEX (FBS 1.0 µM DEX AIM), HI-FBS CM based AIM media with 1.0 µM DEX (HI-FBS 1.0 µM DEX AIM), and Hyclone CM based OIM media with 1.0 µM DEX (Hyclone 1.0 µM DEX OIM).

    Techniques: Transfection, Staining, Cell Counting

    Knockdown of Sp1 Decreases the Myogenic Differentiation of Hypoxic-EBs (A) Generation of Sp1-knockdown stable cells. C57 ESCs were transfected with 1 μg shMock or shSp1 for 24 h, and further selected by puromycin treatment. MyoD protein was remarkably decreased in all four Sp1-knockdown hypoxic-ES clones compared to that in all four control clones transfected with shMock. (B) Stable knockdown cells were formed as EBs by the hanging drop method, cultured under normoxic or hypoxic conditions, and plated onto a gelatin-coated plate in DMEM/10% FBS for 1 day. The culture medium was replaced with SkIM and further incubated for up to 20 days. (C) Muscle regulatory factors (MyoD, myogenin) were increased in Hyp-shMock cells compared to those in Nor-shMock cells, and were significantly decreased to a greater extent in Hyp-shSp1 cells compared to expression in Hyp-shMock cells (n = 6); *p

    Journal: Molecular Therapy

    Article Title: The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells

    doi: 10.1016/j.ymthe.2019.08.014

    Figure Lengend Snippet: Knockdown of Sp1 Decreases the Myogenic Differentiation of Hypoxic-EBs (A) Generation of Sp1-knockdown stable cells. C57 ESCs were transfected with 1 μg shMock or shSp1 for 24 h, and further selected by puromycin treatment. MyoD protein was remarkably decreased in all four Sp1-knockdown hypoxic-ES clones compared to that in all four control clones transfected with shMock. (B) Stable knockdown cells were formed as EBs by the hanging drop method, cultured under normoxic or hypoxic conditions, and plated onto a gelatin-coated plate in DMEM/10% FBS for 1 day. The culture medium was replaced with SkIM and further incubated for up to 20 days. (C) Muscle regulatory factors (MyoD, myogenin) were increased in Hyp-shMock cells compared to those in Nor-shMock cells, and were significantly decreased to a greater extent in Hyp-shSp1 cells compared to expression in Hyp-shMock cells (n = 6); *p

    Article Snippet: For in vitro myogenic-lineage differentiation, EBs were plated on 0.3% gelatin-coated culture dishes in DMEM/10% FBS for 1 day and further incubated, which was followed by replacement with specific media with some modification, specifically SkIM, high-glucose DMEM (GIBCO, Grand Island, NY), 10% FBS (HyClone, Fisher Scientific, Pittsburgh, PA, USA), 5% horse serum (Sigma, St. Louis, MO, USA), 1% penicillin/streptomycin (GIBCO, Grand Island, NY), 1% non-essential amino acids (GIBCO, Grand Island, NY), 0.1 mM BME (Sigma, St. Louis, MO, USA), and recombinant mouse vascular endothelial growth factor (VEGF) (100 ng/mL: R & D Systems, Minneapolis, MN, USA).

    Techniques: Transfection, Clone Assay, Cell Culture, Incubation, Expressing

    Hypoxic Preconditioning Stimulates the Differentiation of mESC-Derived EBs to the Myogenic Lineage (A) EBs were formed from C57 mESCs by the hanging drop method for 3 days, cultured under normoxic or hypoxic conditions for 16 h, and allowed to attach to a 0.3% gelatin-coated plate in DMEM/10% FBS for 1 day; the medium was then changed to fresh media and cells were further differentiated for up to 10 days. (B) The pluripotency marker Oct4 was significantly downregulated compared to expression under conditions of normoxia, and the myogenic marker MyoD was significantly upregulated in hypoxic cells compared to expression in normoxic cells based on real-time PCR analysis. Graphs show the relative percent change (n = 4); *p

    Journal: Molecular Therapy

    Article Title: The MicroRNA-92a/Sp1/MyoD Axis Regulates Hypoxic Stimulation of Myogenic Lineage Differentiation in Mouse Embryonic Stem Cells

    doi: 10.1016/j.ymthe.2019.08.014

    Figure Lengend Snippet: Hypoxic Preconditioning Stimulates the Differentiation of mESC-Derived EBs to the Myogenic Lineage (A) EBs were formed from C57 mESCs by the hanging drop method for 3 days, cultured under normoxic or hypoxic conditions for 16 h, and allowed to attach to a 0.3% gelatin-coated plate in DMEM/10% FBS for 1 day; the medium was then changed to fresh media and cells were further differentiated for up to 10 days. (B) The pluripotency marker Oct4 was significantly downregulated compared to expression under conditions of normoxia, and the myogenic marker MyoD was significantly upregulated in hypoxic cells compared to expression in normoxic cells based on real-time PCR analysis. Graphs show the relative percent change (n = 4); *p

    Article Snippet: For in vitro myogenic-lineage differentiation, EBs were plated on 0.3% gelatin-coated culture dishes in DMEM/10% FBS for 1 day and further incubated, which was followed by replacement with specific media with some modification, specifically SkIM, high-glucose DMEM (GIBCO, Grand Island, NY), 10% FBS (HyClone, Fisher Scientific, Pittsburgh, PA, USA), 5% horse serum (Sigma, St. Louis, MO, USA), 1% penicillin/streptomycin (GIBCO, Grand Island, NY), 1% non-essential amino acids (GIBCO, Grand Island, NY), 0.1 mM BME (Sigma, St. Louis, MO, USA), and recombinant mouse vascular endothelial growth factor (VEGF) (100 ng/mL: R & D Systems, Minneapolis, MN, USA).

    Techniques: Derivative Assay, Cell Culture, Marker, Expressing, Real-time Polymerase Chain Reaction

    The anti-HCC effects by SS in a mouse xenograft tumor model. (A) HepG2-Luc cells carrying luciferase reporter gene (HepG2-Luc, obtained from the Guangzhou Land Biological Technology Co., Guangzhou, China) were resuspended in 0.2 mL of phenol red-free RIPM 1640 with 2% FBS in a number of 2.0 × 10 6 . Then, the resuspended cells were injected into the upper hind limb of the nude mice. Xenografts were expected to grow for 1 week when starting the first measurements. Mice were randomly divided into three groups: the control, low-dose group (SS, 5 mg/kg), and high-dose group (SS, 20 mg/kg), and were injected with reference substance or SS once a day via intraperitoneal injection for up to 15 days ( n = 9 per group). The xenografts were assessed by in vivo bioluminescence imaging at the first and end of the experiments (on day 2 and 15). The tumor growth was monitored by injecting luciferin in the mice followed by measuring bioluminescence using IVIS Imaging System. Imaging and quantification of signals were controlled by the acquisition and analysis software living image as described in the Materials and Methods section. Representative images are shown. (B,C) The xenografts were harvested on day 15, and the weight (B) and volume (C) of tumors were measured. (D) The photographs of the vehicle- and drugs-treated xenografts derived from nude mice are shown. (E–G) At the end of the experiments, xenograft tumors were isolated from individual animals, and the corresponding lysates were processed and detected miR-375-3p and CCAT1 levels, SP1 and IRF5 protein expressions by qRT-PCR and Western blot, respectively. GAPDH was used as a loading control. The figures are representative cropped gels/blots that have been run under the same experimental conditions. The bar graphs represented the tumor weight and volume of mice results of as mean ± SD. * Indicates the significant difference from the untreated control ( p

    Journal: Frontiers in Oncology

    Article Title: The Reciprocal Interaction Between LncRNA CCAT1 and miR-375-3p Contribute to the Downregulation of IRF5 Gene Expression by Solasonine in HepG2 Human Hepatocellular Carcinoma Cells

    doi: 10.3389/fonc.2019.01081

    Figure Lengend Snippet: The anti-HCC effects by SS in a mouse xenograft tumor model. (A) HepG2-Luc cells carrying luciferase reporter gene (HepG2-Luc, obtained from the Guangzhou Land Biological Technology Co., Guangzhou, China) were resuspended in 0.2 mL of phenol red-free RIPM 1640 with 2% FBS in a number of 2.0 × 10 6 . Then, the resuspended cells were injected into the upper hind limb of the nude mice. Xenografts were expected to grow for 1 week when starting the first measurements. Mice were randomly divided into three groups: the control, low-dose group (SS, 5 mg/kg), and high-dose group (SS, 20 mg/kg), and were injected with reference substance or SS once a day via intraperitoneal injection for up to 15 days ( n = 9 per group). The xenografts were assessed by in vivo bioluminescence imaging at the first and end of the experiments (on day 2 and 15). The tumor growth was monitored by injecting luciferin in the mice followed by measuring bioluminescence using IVIS Imaging System. Imaging and quantification of signals were controlled by the acquisition and analysis software living image as described in the Materials and Methods section. Representative images are shown. (B,C) The xenografts were harvested on day 15, and the weight (B) and volume (C) of tumors were measured. (D) The photographs of the vehicle- and drugs-treated xenografts derived from nude mice are shown. (E–G) At the end of the experiments, xenograft tumors were isolated from individual animals, and the corresponding lysates were processed and detected miR-375-3p and CCAT1 levels, SP1 and IRF5 protein expressions by qRT-PCR and Western blot, respectively. GAPDH was used as a loading control. The figures are representative cropped gels/blots that have been run under the same experimental conditions. The bar graphs represented the tumor weight and volume of mice results of as mean ± SD. * Indicates the significant difference from the untreated control ( p

    Article Snippet: Cells were cultured at 37°C in 5% CO2 in RPMI-1640 medium (GIBCO, Life Technologies, Grand Island, NY, USA) supplemented with 10% FBS (HyClone, Invitrogen, Camarillo, CA, USA), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA).

    Techniques: Luciferase, Injection, Mouse Assay, In Vivo, Imaging, Software, Derivative Assay, Isolation, Quantitative RT-PCR, Western Blot