fbs  (Corning Life Sciences)

Journal: Human Gene Therapy Methods

Article Title: Sodium Chloride Enhances Recombinant Adeno-Associated Virus Production in a Serum-Free Suspension Manufacturing Platform Using the Herpes Simplex Virus System

doi: 10.1089/hgtb.2016.151

Figure Lengend Snippet: Increased rAAV9 production after sodium chloride supplementation for other rAAV serotypes, genes of interest, and/or cell types. Total transducing units (A) and total vector genomes (B) were evaluated for rAAV2-GFP, rAAV5-GFP, and rAAV9-Des-GAA produced by rHSV coinfection in shaker flasks (50-ml working volume) in the presence of increased sodium chloride concentration ( n = 2 for all). (C) Impact of increased sodium chloride on rAAV9-GFP production in BHK cells grown in DMEM with 5% FBS (BHK) or adapted to serum-free medium (BHK-SFX). (D) Total rAAV9-GFP vector genomes produced in BHK and BHK-SFX cells by rHSV coinfection in the absence or presence of optimal salt supplementation (30 and 60 m M , respectively). * p

Article Snippet: Cell lines Suspension BHK21C13-2P (BHK) cells were obtained from Sigma-Aldrich (St. Louis, MO) and maintained in Dulbecco's modified Eagle's medium (DMEM; HyClone/GE Healthcare Life Sciences, Logan, UT) supplemented with 5% FBS (Corning, Corning, NY) and 1% antibiotic/antimycotic (Gibco/Thermo Fisher Scientific, Waltham, MA).

Techniques: Plasmid Preparation, Produced, Concentration Assay

Journal: Human Gene Therapy Methods

Article Title: Sodium Chloride Enhances Recombinant Adeno-Associated Virus Production in a Serum-Free Suspension Manufacturing Platform Using the Herpes Simplex Virus System

doi: 10.1089/hgtb.2016.151

Figure Lengend Snippet: Characterization of rAAV vector made in Expi293F suspension system. (A and B) rAAV9-GFP was produced by rHSV infection in adherent HEK293 cells grown in 10-chamber CellSTACKs (CS10) under optimal conditions (approximately 3 × 10 9 cells) either in DMEM containing 5% FBS alone ( n = 4) or supplemented with 60 m M sodium chloride ( n = 6), or in a 3-liter suspension culture containing 3 × 10 9 Expi293F cells ( n = 5). Total vector genome (A) and transducing unit (B) yields are shown. Total purified vector genomes are represented relative to units produced per cell (C) and per liter of medium (D) . (E) The vector genome-to-transducing unit (VG/TU) ratio was calculated for each sample, and the averaged values for each group are shown. (F) Coomassie staining of rAAV9-GFP final stocks produced in HEK293 CS10 in the absence of salt supplementation (lane 1), or supplemented with sodium chloride (lane 2), or produced in Expi293F 3-liter cultures supplemented with sodium chloride (lanes 3–7). A total of 10 11 VG was loaded per well. * p

Article Snippet: Cell lines Suspension BHK21C13-2P (BHK) cells were obtained from Sigma-Aldrich (St. Louis, MO) and maintained in Dulbecco's modified Eagle's medium (DMEM; HyClone/GE Healthcare Life Sciences, Logan, UT) supplemented with 5% FBS (Corning, Corning, NY) and 1% antibiotic/antimycotic (Gibco/Thermo Fisher Scientific, Waltham, MA).

Techniques: Plasmid Preparation, Produced, Infection, Purification, Staining

Journal: Journal of Nanobiotechnology

Article Title: Polymer coated gold-ferric oxide superparamagnetic nanoparticles for theranostic applications

doi: 10.1186/s12951-018-0405-7

Figure Lengend Snippet: Surface zeta potential of a Au–Fe 3 O 4 , and b PLL–Au–Fe 3 O 4 NPs in water, PBS, RPMI 1640 cell culture medium and PBS containing 10% FBS. Three colored (red, green and blue) lines indicate three replicates from three independent experiments. Multiple peaks in Au–Fe 3 O 4 ( a ) indicate their aggregation behavior that disappears after PLL coating ( b )

Article Snippet: BT-474 and MDA-MB-231 breast cancer cells (ATCC) were cultured at 37 °C and 5% CO2 in Hybri-Care (ATCC) and RPMI 1640 (Life Technologies), respectively supplemented with 10% FBS (Corning) and 1% (100 units/ml) Penicillin–Streptomycin (Gibco).

Techniques: Cell Culture

Journal: bioRxiv

Article Title: Methionine synthase is essential for cancer cell proliferation in physiological folate environments

doi: 10.1101/2020.06.12.149005

Figure Lengend Snippet: Characterization of cells cultured in 5-methyl THF. (A) Histogram of MTR mRNA expression levels in cell lines as reported in The Cancer Genome Atlas 23 . (B) Proliferation rate of T.T cells in media with the indicated folate before (no pre-starvation) or after 3 days of culture with no folic acid (pre-starvation). (C) LC/MS measurement of folic acid and 5-methyl THF concentrations present in the dialyzed fetal bovine serum used to prepare culture medium for this study (batch 1; see Methods). (D) LC/MS measurement of intracellular methionine, lysine, and serine levels in A549 and T.T cells cultured for the indicated number of days in media lacking any added folates. LC/MS measurement of intracellular SAM and SAH levels (E) and nucleotide levels (F) in A549 and T.T cells cultured for the indicated number of days in media lacking any added folates. (G) Proliferation rate of A549 or T.T cells cultured in media lacking any added folates for the indicated number of days. Mean +/- SD is displayed for all panels. p values indicated on all panels are derived from two-tailed, unpaired Welch’s t tests. For all LC/MS measurements, data are normalized to cell number and to an internal standard.

Article Snippet: For experiments, cells were grown in RPMI-1640 without phenol red with 10% FBS that has been dialyzed to remove small molecules (Gibco, 26400044; batch 1: Lot #1841165, batch 2: Lot #2093857).

Techniques: Cell Culture, Expressing, Liquid Chromatography with Mass Spectroscopy, Derivative Assay, Two Tailed Test

Journal: Biomaterials

Article Title: Rapid Determination of Plasmonic Nanoparticle Agglomeration Status in Blood

doi: 10.1016/j.biomaterials.2015.01.072

Figure Lengend Snippet: The agglomeration behavior of citrate-capped AuNPs in four aqueous media: (A) H 2 O; (B) phosphate-buffered saline; (C) cell-culture medium; and (D) fetal bovine serum (black) with and (grey) without AuNPs. (Left) UV-Vis spectra; (Center) darkfield image;

Article Snippet: Phosphate buffered saline (PBS), fetal bovine serum (FBS), and Eagle medium were acquired from Corning Cellgro (Manassas, VA).

Techniques: Cell Culture