Structured Review

Avantor fbs
Potassium uptake was decreased by SCZ astrocytes A, The Na + /K + -ATPase pump, NKCC1 Na + /K + /2Cl − cotransporter, and inwardly rectifying K + channels are all involved in the regulation of potassium uptake by astrocytes. B, qPCR confirmed that several K + channel-associated genes were down-regulated in SCZ <t>CD44</t> + astrocyte-biased GPCs relative to CTR cells. C, SCZ and CTR CD44 + GPCs were cultured in <t>FBS</t> with BMP4 to produce mature GFAP + astrocytes, which were then assessed for K + uptake; the results were normalized to both total protein and cell number. K + uptake by SCZ astrocytes was significantly reduced (4 SCZ lines, 5 repeats/each line), compared to K + uptake by CTR astrocytes (4 CTR lines, 5 repeats/each line). D-E, Astrocytes were treated with ouabain, bumetanide, and tertiapin to assess which potassium transporter classes were functionally impaired in SCZ astrocytes relative to control (4 lines of each, 4 repeats/line). Both ouabain and bumetanide efficiently decreased K + uptake by CTR astrocytes ( D , gray bars), whereas neither affected K + uptake by SCZ astrocytes ( E , purple bars). *P
Fbs, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 72 article reviews
Price from $9.99 to $1999.99
fbs - by Bioz Stars, 2020-08
92/100 stars

Images

1) Product Images from "Dysregulated glial differentiation in schizophrenia may be relieved by suppression of SMAD4 and REST-dependent signaling"

Article Title: Dysregulated glial differentiation in schizophrenia may be relieved by suppression of SMAD4 and REST-dependent signaling

Journal: Cell reports

doi: 10.1016/j.celrep.2019.05.088

Potassium uptake was decreased by SCZ astrocytes A, The Na + /K + -ATPase pump, NKCC1 Na + /K + /2Cl − cotransporter, and inwardly rectifying K + channels are all involved in the regulation of potassium uptake by astrocytes. B, qPCR confirmed that several K + channel-associated genes were down-regulated in SCZ CD44 + astrocyte-biased GPCs relative to CTR cells. C, SCZ and CTR CD44 + GPCs were cultured in FBS with BMP4 to produce mature GFAP + astrocytes, which were then assessed for K + uptake; the results were normalized to both total protein and cell number. K + uptake by SCZ astrocytes was significantly reduced (4 SCZ lines, 5 repeats/each line), compared to K + uptake by CTR astrocytes (4 CTR lines, 5 repeats/each line). D-E, Astrocytes were treated with ouabain, bumetanide, and tertiapin to assess which potassium transporter classes were functionally impaired in SCZ astrocytes relative to control (4 lines of each, 4 repeats/line). Both ouabain and bumetanide efficiently decreased K + uptake by CTR astrocytes ( D , gray bars), whereas neither affected K + uptake by SCZ astrocytes ( E , purple bars). *P
Figure Legend Snippet: Potassium uptake was decreased by SCZ astrocytes A, The Na + /K + -ATPase pump, NKCC1 Na + /K + /2Cl − cotransporter, and inwardly rectifying K + channels are all involved in the regulation of potassium uptake by astrocytes. B, qPCR confirmed that several K + channel-associated genes were down-regulated in SCZ CD44 + astrocyte-biased GPCs relative to CTR cells. C, SCZ and CTR CD44 + GPCs were cultured in FBS with BMP4 to produce mature GFAP + astrocytes, which were then assessed for K + uptake; the results were normalized to both total protein and cell number. K + uptake by SCZ astrocytes was significantly reduced (4 SCZ lines, 5 repeats/each line), compared to K + uptake by CTR astrocytes (4 CTR lines, 5 repeats/each line). D-E, Astrocytes were treated with ouabain, bumetanide, and tertiapin to assess which potassium transporter classes were functionally impaired in SCZ astrocytes relative to control (4 lines of each, 4 repeats/line). Both ouabain and bumetanide efficiently decreased K + uptake by CTR astrocytes ( D , gray bars), whereas neither affected K + uptake by SCZ astrocytes ( E , purple bars). *P

Techniques Used: Real-time Polymerase Chain Reaction, Cell Culture

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Incubation:

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Cell Culture:

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Co-Culture Assay:

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    Fbs, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fbs/product/Avantor
    Average 92 stars, based on 115 article reviews
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