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Atlanta Biologicals fbs
Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fbs/product/Atlanta Biologicals
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fbs - by Bioz Stars, 2021-03
86/100 stars

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Cell Culture:

Article Title: Essential Role of NADPH Oxidase-Dependent Reactive Oxygen Species Generation in Regulating MicroRNA-21 Expression and Function in Prostate Cancer
Article Snippet: Antibodies against p47phox , maspin, and t-Akt were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA); while those against β-actin, PDCD4, and p-Akt were from Sigma Chemical Co., Epitomics, Inc. (Burlingame, CA) and Cell Signaling Technology, Inc. (Danvers, MA), respectively. .. Cell culture media RPMI 1640 and penicillin/streptomycin were obtained from Invitrogen (Carlsbad, CA), while fetal bovine serum (FBS) was from Atlanta Biologicals, Inc. (Lawrenceville, GA). .. Human prostate cancer TissueScan qPCR panels with the clinical information, including the disease stages, were obtained from Origene Technologies (Rockville, MD), and FFPE sections for human prostate cancer were purchased from US Biomax, Inc. (Rockville, MD).

Article Title: Trastuzumab Sensitizes Ovarian Cancer Cells to EGFR-targeted Therapeutics
Article Snippet: Anti-β-tubulin antibody was purchased from Cell Signaling Technology. .. Cell culture media and all culture supplements were purchased from Mediatech, except for fetal bovine serum (FBS), which was purchased from Atlanta Biologicals, and G418, which was purchased from GibcoBRL. .. Cetuximab was obtained from Bristol Myers Squibb, trastuzumab was obtained from Genentech, and erlotinib, gefitinib, and lapatinib were obtained from Chemitek.

other:

Article Title: Saturated fatty acids activate TLR-mediated proinflammatory signaling pathways [S]
Article Snippet: FBSs were purchased as follows: premium select FBS (Catalog #: S11595 and S11550, Lot #: K0109) and Optima FBS (Catalog #: S12495, Lot #: L0019) from Atlanta Biologicals (Lawrenceville, GA), Hyclone FBS (Catalog #: SH30071, Lot #: ATD31956) from HyClone (Logan, UT), Gibco FBS (Catalog #: 10082, Lot #: 544122) from Invitrogen (Carlsbad, CA), and Atlas FBS (Catalog #: F-0500-A, Lot #: 80814) from Atlas Biologicals (Fort Collins, CO).

Incubation:

Article Title: Synthesis and Evaluation of an Anti-MLC1 x Anti-CD90 Bispecific Antibody for Targeting and Retaining Bone-Marrow Derived Multipotent Stromal Cells in Infarcted Myocardium
Article Snippet: Chondro-, osteo-, and adipogenesis differentiation assays were performed as previously described., Briefly, monolayer cultures for osteogenesis and adipogenesis assays were initiated by seeding 2 × 103 cells in 6-well tissue culture plates in αMEM. .. At 70% confluence, adipogenesis was induced by incubation with αMEM containing 10 mg/mL insulin, 10% FBS (v/v), 1 mM dexamethasone, 0.5 mM methyl-isobutylxanthine, and 100 mM indomethacine for 3 d, followed by 3 d in adipogenic maintenance medium (αMEM containing 10 mg/mL insulin and 10% (v/v) FBS). .. Oil Red staining of neutral lipids was used to denote adipocytes.

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  • 86
    Atlanta Biologicals exosome depleted fbs
    Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either <t>exosome-depleted</t> <t>FBS</t> (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).
    Exosome Depleted Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosome depleted fbs/product/Atlanta Biologicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exosome depleted fbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Atlanta Biologicals fetal bovine serum fbs
    MQ-induced changes in cellular ATP and cytosolic lactate. HT29 cells (1 × 10 4 ) were seeded in 96-well plates and grown overnight at 37°C. The next day, the culture media were replaced by fresh phenol-red and fetal bovine serum-free <t>Dulbecco's</t> modified essential medium containing 1.2, 4.6, or 18.3 m M glucose, and the cells were exposed to 50 or 200 μ M MQ. (A) In time course experiments, cells were treated with MQ for 0–60 min. ATP was measured with a luciferin/luciferase bioluminescence Promega kit according to manufacturer's protocol. Each experiment was conducted in quadruplicates and ATP contents are expressed as percent relative to untreated controls (100%). Results are mean ± SEM for six cell preparations. (B) The contribution of mitochondria-derived ( upper panel ) and glycolysis-derived ( bottom panel ) ATP to total ATP levels was determined in cells treated with antimycin A (2 μ M ) or 2-deoxyglucose (2DG), respectively. Concentrations of 2DG, namely, 3, 11.5, and 45.8 m M were 2.5 × higher than the respective glucose concentrations. Results are mean ± SEM for n = 6 for 18.3 m M glucose, and n = 4 each for 4.6 and 1.2 m M glucose. * p
    Fetal Bovine Serum Fbs, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal bovine serum fbs/product/Atlanta Biologicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum fbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either exosome-depleted FBS (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).

    Journal: PLoS ONE

    Article Title: Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone

    doi: 10.1371/journal.pone.0165326

    Figure Lengend Snippet: Trabecular meshwork (TM) exosomes bind fibronectin on the external surface. Serum-free medium was conditioned for 2.5 hours with primary TM cell cultures. Conditioned media was split in half and either exosome-depleted FBS (10% final v:v) or purified human plasma fibronectin (Fn, 2.5μg/ml [final]) was added to one portion. Exosomes were prepared from both portions (and from unconditioned media + FBS or purified Fn) and Fn content was assessed by Western blot. The blots shown represent the results from a minimum 3 biological replicates (cell strains isolated from different human donors).

    Article Snippet: In some experiments, purified human plasma fibronectin (#F0895, Sigma-Aldrich) or exosome-depleted FBS (#S11550, Atlanta Biologicals, Lawrenceville, GA; FBS spun overnight at 100,000 g ) was added to cleared supernatant for a final concentration of 2.5 μg/ml or 10% v:v, respectively.

    Techniques: Purification, Western Blot, Isolation

    MQ-induced changes in cellular ATP and cytosolic lactate. HT29 cells (1 × 10 4 ) were seeded in 96-well plates and grown overnight at 37°C. The next day, the culture media were replaced by fresh phenol-red and fetal bovine serum-free Dulbecco's modified essential medium containing 1.2, 4.6, or 18.3 m M glucose, and the cells were exposed to 50 or 200 μ M MQ. (A) In time course experiments, cells were treated with MQ for 0–60 min. ATP was measured with a luciferin/luciferase bioluminescence Promega kit according to manufacturer's protocol. Each experiment was conducted in quadruplicates and ATP contents are expressed as percent relative to untreated controls (100%). Results are mean ± SEM for six cell preparations. (B) The contribution of mitochondria-derived ( upper panel ) and glycolysis-derived ( bottom panel ) ATP to total ATP levels was determined in cells treated with antimycin A (2 μ M ) or 2-deoxyglucose (2DG), respectively. Concentrations of 2DG, namely, 3, 11.5, and 45.8 m M were 2.5 × higher than the respective glucose concentrations. Results are mean ± SEM for n = 6 for 18.3 m M glucose, and n = 4 each for 4.6 and 1.2 m M glucose. * p

    Journal: Antioxidants & Redox Signaling

    Article Title: Disruption of Pyridine Nucleotide Redox Status During Oxidative Challenge at Normal and Low-Glucose States: Implications for Cellular Adenosine Triphosphate, Mitochondrial Respiratory Activity, and Reducing Capacity in Colon Epithelial Cells

    doi: 10.1089/ars.2010.3489

    Figure Lengend Snippet: MQ-induced changes in cellular ATP and cytosolic lactate. HT29 cells (1 × 10 4 ) were seeded in 96-well plates and grown overnight at 37°C. The next day, the culture media were replaced by fresh phenol-red and fetal bovine serum-free Dulbecco's modified essential medium containing 1.2, 4.6, or 18.3 m M glucose, and the cells were exposed to 50 or 200 μ M MQ. (A) In time course experiments, cells were treated with MQ for 0–60 min. ATP was measured with a luciferin/luciferase bioluminescence Promega kit according to manufacturer's protocol. Each experiment was conducted in quadruplicates and ATP contents are expressed as percent relative to untreated controls (100%). Results are mean ± SEM for six cell preparations. (B) The contribution of mitochondria-derived ( upper panel ) and glycolysis-derived ( bottom panel ) ATP to total ATP levels was determined in cells treated with antimycin A (2 μ M ) or 2-deoxyglucose (2DG), respectively. Concentrations of 2DG, namely, 3, 11.5, and 45.8 m M were 2.5 × higher than the respective glucose concentrations. Results are mean ± SEM for n = 6 for 18.3 m M glucose, and n = 4 each for 4.6 and 1.2 m M glucose. * p

    Article Snippet: Antibiotic/antimycotic, trypsin, L-glutamine, McCoys' medium, and Dulbecco's modified essential medium (DMEM) were from Gibco Corporation, and fetal bovine serum (FBS) was from Atlanta Biologicals.

    Techniques: Modification, Luciferase, Derivative Assay

    Melanoma cells display invasive properties in vitro. Melanoma cells, 2.5 X 10 4 cells, were resuspended in RPMI (500µl) containing 1% FBS and allowed to invade for 18 hours. All of the primary melanoma cells (MEL-2, MEL-V, 3MM, KFM, and GLM-2) displayed differential invasive properties as did the established melanoma cell (SK-Mel-28, SK-Mel-37, and SK-Mel-103). The data is represented as invasion index, which is calculated as the percent of cells invading through the growth factor reduced matrigel invasion chambers relative to the cells migrating through control membrane. Each experiment was repeated three times with similar results observed and the standard deviation shown above each sample represents three replicates in one experiment.

    Journal: Journal of Cancer

    Article Title: Ex Vivo Derived Primary Melanoma Cells: Implications for Immunotherapeutic Vaccines

    doi: 10.7150/jca.6625

    Figure Lengend Snippet: Melanoma cells display invasive properties in vitro. Melanoma cells, 2.5 X 10 4 cells, were resuspended in RPMI (500µl) containing 1% FBS and allowed to invade for 18 hours. All of the primary melanoma cells (MEL-2, MEL-V, 3MM, KFM, and GLM-2) displayed differential invasive properties as did the established melanoma cell (SK-Mel-28, SK-Mel-37, and SK-Mel-103). The data is represented as invasion index, which is calculated as the percent of cells invading through the growth factor reduced matrigel invasion chambers relative to the cells migrating through control membrane. Each experiment was repeated three times with similar results observed and the standard deviation shown above each sample represents three replicates in one experiment.

    Article Snippet: SK-Mel-28, SK-Mel-37, and SK-Mel-103 are established human melanoma cell line, which were generously gifted to us by Jedd D. Wolchok, MD, PhD (Memorial Sloan-Kettering Cancer Center, New york, NY) and were cultured in RPMI supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals, Lawrenceville, GA), 2mM L-glutamine (Mediatech), penicillin 10,000IU/ml, streptomycin 10,000ug/ml, and Normocin™ 100μg/ml (Invivogen).

    Techniques: In Vitro, Standard Deviation

    Comparison of cDNA-derived MR766 and FSS13025 virus growth. Vero cells (2.5 × 10 5 /well) in 12-well plates were co-transfected with 1 µg pTight-ZIKV/MR766 or pTight-ZIKV/FSS13025 DNA and 1 µg pTet-On vector, followed by the addition of 1 µg/mL Doxycycline to cell culture media. Infectious virus titers in the supernatants collected at day 3 (MR766) or day 6 (FSS13025) were quantified by a plaque assay. ( A ) Plaque formation by cDNA-derived ZIKV/MR766 and ZIKV/FSS13025 viruses. Vero cells in 12-well cell culture plate were infected with 30 PFU of either MR766 or FSS13025. Plaques were stained and visualized after 4 days (for MR766) or 6 days (for FSS13025) p.i. ( B ) Growth curves of cDNA-derived MR766 and FSS13025 viruses. To compare the growth ability between MR766 and FSS13025, 4 × 10 5 Vero cells seeded in 6-well plates were infected with MR766 or FSS13025 virus at 0.01 MOI at 37 °C for 1 h. Upon washing with phosphate-buffered saline (PBS) three times, infected Vero cells were incubated with 2 mL DMEM containing 10% FBS. At day 1, 2, and 3 post-infection, virus in the supernatant was collected and stored at −80 °C. Virus yields at different time points were determined by a plaque assay. Data points represent the mean titer of triplicates. Statistical significance was analyzed by Student’s t -test: ** p

    Journal: Viruses

    Article Title: Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains

    doi: 10.3390/v10120700

    Figure Lengend Snippet: Comparison of cDNA-derived MR766 and FSS13025 virus growth. Vero cells (2.5 × 10 5 /well) in 12-well plates were co-transfected with 1 µg pTight-ZIKV/MR766 or pTight-ZIKV/FSS13025 DNA and 1 µg pTet-On vector, followed by the addition of 1 µg/mL Doxycycline to cell culture media. Infectious virus titers in the supernatants collected at day 3 (MR766) or day 6 (FSS13025) were quantified by a plaque assay. ( A ) Plaque formation by cDNA-derived ZIKV/MR766 and ZIKV/FSS13025 viruses. Vero cells in 12-well cell culture plate were infected with 30 PFU of either MR766 or FSS13025. Plaques were stained and visualized after 4 days (for MR766) or 6 days (for FSS13025) p.i. ( B ) Growth curves of cDNA-derived MR766 and FSS13025 viruses. To compare the growth ability between MR766 and FSS13025, 4 × 10 5 Vero cells seeded in 6-well plates were infected with MR766 or FSS13025 virus at 0.01 MOI at 37 °C for 1 h. Upon washing with phosphate-buffered saline (PBS) three times, infected Vero cells were incubated with 2 mL DMEM containing 10% FBS. At day 1, 2, and 3 post-infection, virus in the supernatant was collected and stored at −80 °C. Virus yields at different time points were determined by a plaque assay. Data points represent the mean titer of triplicates. Statistical significance was analyzed by Student’s t -test: ** p

    Article Snippet: Vero and C6/36 (CRL-1660) cell lines were obtained from America type culture collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals, Atlanta, GA, USA), 0.1 mM nonessential amino acids, penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO, USA and HyClone, Logan, UT, USA) at 37 °C in a 5% CO2 incubator.

    Techniques: Derivative Assay, Transfection, Plasmid Preparation, Cell Culture, Plaque Assay, Infection, Staining, Incubation