fatty acid free bovine serum albumin  (Thermo Fisher)


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    Name:
    Bovine Serum Albumin
    Description:
    Bovine Serum Albumin is suitable for use as an enzyme stabilizer during purification or for dilution of restriction endonucleases and nucleic acid modifying enzymes BSA is also commonly used in DNA and protein labeling experiments as a blocking agent to minimize background This BSA is acetylated to inactivate contaminating nucleases and proteases Performance and quality testingPurity is determined by SDS PAGE No detectable contaminating activity is observed in 3 and 5 exonuclease DNA nicking and restriction endonuclease inhibition assays
    Catalog Number:
    15561020
    Price:
    None
    Applications:
    ChIP-on-Chip|Cloning|RNAi, Epigenetics & Non-Coding RNA Research|Chromatin Biology|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher fatty acid free bovine serum albumin
    Bovine Serum Albumin is suitable for use as an enzyme stabilizer during purification or for dilution of restriction endonucleases and nucleic acid modifying enzymes BSA is also commonly used in DNA and protein labeling experiments as a blocking agent to minimize background This BSA is acetylated to inactivate contaminating nucleases and proteases Performance and quality testingPurity is determined by SDS PAGE No detectable contaminating activity is observed in 3 and 5 exonuclease DNA nicking and restriction endonuclease inhibition assays
    https://www.bioz.com/result/fatty acid free bovine serum albumin/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    fatty acid free bovine serum albumin - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Endo-Lysosomal Dysfunction in Human Proximal Tubular Epithelial Cells Deficient for Lysosomal Cystine Transporter Cystinosin
    Article Snippet: .. Briefly, control and cystinosis ciPTEC grown in glass-bottom cell culture chambers (LabTek) were incubated with 5 μg/ml DQ-BSA and 5 μg/ml Alexa-Fluor-555-BSA (Life Technologies) at 37°C for 2 h, washed and analyzed under a confocal microscope. ..

    Immunocytochemistry:

    Article Title: CO-LOCALIZATION OF THE VANILLOID CAPSAICIN RECEPTOR AND SUBSTANCE P IN SENSORY NERVE FIBERS INNERVATING COCHLEAR AND VERTEBRO-BASILAR ARTERIES
    Article Snippet: .. The tissues were washed in fresh 0.02 M PBS (pH 7.4), permeabilized in 0.5% Triton-X 100 (Sigma, St. Louis, MO, USA) in 0.02 M PBS (pH 7.3) for 30 min, and immunoblocked in 10% goat serum in 1% bovine serum albumin (BSA) in 0.02 M PBS (1% BSA-PBS) for 60 min. For immunocytochemistry, tissues were incubated with rabbit anti-TRPV1 antiserum (diluted 1:1000 in 1% BSA-PBS; gift from Dr. Michael J Caterina, John Hopkins University) for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated in Alexa-488-conjugated goat anti-rabbit IgG for 3 h (Molecular Probes; 1:100 in BSA-PBS). .. Alternatively, tissues were incubated in rat anti-SP antibody (diluted in 1:100 in 1% BSA-PBS; Sigma) for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated in Alexa-568-conjugated goat anti-rat IgG for 3 h (Molecular Probes; 1:100 in BSA-PBS).

    Ligation:

    Article Title: Glycoproteomics enabled by tagging sialic acid- or galactose-terminated glycans
    Article Snippet: .. For the oxime ligation step, cells were suspended to 1 × 107 cells/mL in PBS/5% BSA, pH 6.7 containing 250 μM aminooxy-biotin, aminooxy-AF488 (Invitrogen Corporation) or aminooxy-FLAG peptide and 10 mM aniline (Sigma–Aldrich) for 90 min at 4°C with end-over-end mixing. ..

    Cell Culture:

    Article Title: Endo-Lysosomal Dysfunction in Human Proximal Tubular Epithelial Cells Deficient for Lysosomal Cystine Transporter Cystinosin
    Article Snippet: .. Briefly, control and cystinosis ciPTEC grown in glass-bottom cell culture chambers (LabTek) were incubated with 5 μg/ml DQ-BSA and 5 μg/ml Alexa-Fluor-555-BSA (Life Technologies) at 37°C for 2 h, washed and analyzed under a confocal microscope. ..

    Incubation:

    Article Title: Anopheles gambiae Lacking AgTRIO Inefficiently Transmits Plasmodium berghei to Mice
    Article Snippet: .. To determine whether AgTRIO was bound to the sporozoites, the sporozoites were incubated with 1 μg/ml of BSA or AgTRIO on ice for 1 h. The sporozoites were then fixed as mentioned above, followed by incubation with a fluorescein isothiocyanate-labeled anti-His tag monoclonal antibody (3D5; Thermo Fisher Scientific) on ice for 1 h. Sporozoites were viewed using the EVOS FL Auto cell imaging system. ..

    Article Title: Endo-Lysosomal Dysfunction in Human Proximal Tubular Epithelial Cells Deficient for Lysosomal Cystine Transporter Cystinosin
    Article Snippet: .. Briefly, control and cystinosis ciPTEC grown in glass-bottom cell culture chambers (LabTek) were incubated with 5 μg/ml DQ-BSA and 5 μg/ml Alexa-Fluor-555-BSA (Life Technologies) at 37°C for 2 h, washed and analyzed under a confocal microscope. ..

    Article Title: CO-LOCALIZATION OF THE VANILLOID CAPSAICIN RECEPTOR AND SUBSTANCE P IN SENSORY NERVE FIBERS INNERVATING COCHLEAR AND VERTEBRO-BASILAR ARTERIES
    Article Snippet: .. Alternatively, tissues were incubated in rat anti-SP antibody (diluted in 1:100 in 1% BSA-PBS; Sigma) for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated in Alexa-568-conjugated goat anti-rat IgG for 3 h (Molecular Probes; 1:100 in BSA-PBS). .. For double-labeling imunocytochemistry, tissues were incubated in 1% BSA-PBS with 1:1000 rabbit anti-TRPV1 antibody and 1:100 rat anti-SP antibody (Harlan-Sera Labs, Limited, Loughborough, UK) for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated in Alexa-488-conjugated goat anti-rabbit IgG and Alexa-568-conjugated goat anti-rat IgG for 3 h (both diluted 1:100 in BSA-PBS).

    Article Title: CO-LOCALIZATION OF THE VANILLOID CAPSAICIN RECEPTOR AND SUBSTANCE P IN SENSORY NERVE FIBERS INNERVATING COCHLEAR AND VERTEBRO-BASILAR ARTERIES
    Article Snippet: .. The tissues were washed in fresh 0.02 M PBS (pH 7.4), permeabilized in 0.5% Triton-X 100 (Sigma, St. Louis, MO, USA) in 0.02 M PBS (pH 7.3) for 30 min, and immunoblocked in 10% goat serum in 1% bovine serum albumin (BSA) in 0.02 M PBS (1% BSA-PBS) for 60 min. For immunocytochemistry, tissues were incubated with rabbit anti-TRPV1 antiserum (diluted 1:1000 in 1% BSA-PBS; gift from Dr. Michael J Caterina, John Hopkins University) for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated in Alexa-488-conjugated goat anti-rabbit IgG for 3 h (Molecular Probes; 1:100 in BSA-PBS). .. Alternatively, tissues were incubated in rat anti-SP antibody (diluted in 1:100 in 1% BSA-PBS; Sigma) for 48 h. After washing in 1% BSA-PBS, tissues were subsequently incubated in Alexa-568-conjugated goat anti-rat IgG for 3 h (Molecular Probes; 1:100 in BSA-PBS).

    Imaging:

    Article Title: Anopheles gambiae Lacking AgTRIO Inefficiently Transmits Plasmodium berghei to Mice
    Article Snippet: .. To determine whether AgTRIO was bound to the sporozoites, the sporozoites were incubated with 1 μg/ml of BSA or AgTRIO on ice for 1 h. The sporozoites were then fixed as mentioned above, followed by incubation with a fluorescein isothiocyanate-labeled anti-His tag monoclonal antibody (3D5; Thermo Fisher Scientific) on ice for 1 h. Sporozoites were viewed using the EVOS FL Auto cell imaging system. ..

    Staining:

    Article Title: Dapper homolog 1 alpha suppresses metastasis ability of gastric cancer through inhibiting planar cell polarity pathway
    Article Snippet: .. The cover slips with spreading cells were fixed with 3% paraformaldehyde (in PBS) at room temperature for 15 min. Then the cells were washed three times with 1 × TBS and permeabilized with 0.1% Triton X-100 at room temperature for 5 min. Then cells were washed three times with 1 × TBS and blocked with 1% BSA at room temperature for 30 min. For F-actin staining, cells were stained with Rhodamine phalloidin (1:40 dilution in 0.2% BSA) (Invitrogen) at 37°C for 20 min in dark. .. Finally, were washed and mounted with Mounting Medium containing DAPI (Vector Laboratories).

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  • 99
    Thermo Fisher faf bsa
    Competition affinity experiments with <t>FAF-BSA.</t> Global curve fitting of the three affinity experiments used to determine the equilibrium dissociation constants for the individual LPA species (A–E) or S1P (F) binding the antibody or FAF-BSA in solution.
    Faf Bsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/faf bsa/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    faf bsa - by Bioz Stars, 2020-09
    99/100 stars
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    97
    Thermo Fisher bsa fraction v igg free fatty acid poor custom product
    Competition affinity experiments with <t>FAF-BSA.</t> Global curve fitting of the three affinity experiments used to determine the equilibrium dissociation constants for the individual LPA species (A–E) or S1P (F) binding the antibody or FAF-BSA in solution.
    Bsa Fraction V Igg Free Fatty Acid Poor Custom Product, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa fraction v igg free fatty acid poor custom product/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsa fraction v igg free fatty acid poor custom product - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    93
    Thermo Fisher fatty acid free bsa
    Competition affinity experiments with <t>FAF-BSA.</t> Global curve fitting of the three affinity experiments used to determine the equilibrium dissociation constants for the individual LPA species (A–E) or S1P (F) binding the antibody or FAF-BSA in solution.
    Fatty Acid Free Bsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fatty acid free bsa/product/Thermo Fisher
    Average 93 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    fatty acid free bsa - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Competition affinity experiments with FAF-BSA. Global curve fitting of the three affinity experiments used to determine the equilibrium dissociation constants for the individual LPA species (A–E) or S1P (F) binding the antibody or FAF-BSA in solution.

    Journal: Journal of Lipid Research

    Article Title: A novel approach for measuring sphingosine-1-phosphate and lysophosphatidic acid binding to carrier proteins using monoclonal antibodies and the Kinetic Exclusion Assay [S]

    doi: 10.1194/jlr.D068866

    Figure Lengend Snippet: Competition affinity experiments with FAF-BSA. Global curve fitting of the three affinity experiments used to determine the equilibrium dissociation constants for the individual LPA species (A–E) or S1P (F) binding the antibody or FAF-BSA in solution.

    Article Snippet: For S1P experiments, samples containing either 1 or 10 nM LT1009 and 1 or 500 μM FAF-BSA (dependent on experiment) in S1P running buffer were prepared in silanized glass tubes (Thermo Scientific).

    Techniques: Binding Assay

    Error curves for FAF-BSA experiments. Competition n-curve error curves for the experiments in . The K d values for LPA (A–E) and S1P (F) binding either the antibody ( K d 1 , red dotted curve) or FAF-BSA ( K d 2 , black dotted curve) correspond to

    Journal: Journal of Lipid Research

    Article Title: A novel approach for measuring sphingosine-1-phosphate and lysophosphatidic acid binding to carrier proteins using monoclonal antibodies and the Kinetic Exclusion Assay [S]

    doi: 10.1194/jlr.D068866

    Figure Lengend Snippet: Error curves for FAF-BSA experiments. Competition n-curve error curves for the experiments in . The K d values for LPA (A–E) and S1P (F) binding either the antibody ( K d 1 , red dotted curve) or FAF-BSA ( K d 2 , black dotted curve) correspond to

    Article Snippet: For S1P experiments, samples containing either 1 or 10 nM LT1009 and 1 or 500 μM FAF-BSA (dependent on experiment) in S1P running buffer were prepared in silanized glass tubes (Thermo Scientific).

    Techniques: Binding Assay