fatty acid free bovine serum albumin bsa  (Abcam)

 
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  • 99
    Name:
    Bovine Serum Albumin nitrated
    Description:

    Catalog Number:
    ab131379
    Price:
    None
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    Structured Review

    Abcam fatty acid free bovine serum albumin bsa

    https://www.bioz.com/result/fatty acid free bovine serum albumin bsa/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fatty acid free bovine serum albumin bsa - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Development of vaccine for dyslipidemia targeted to a proprotein convertase subtilisin/kexin type 9 (PCSK9) epitope in mice
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) PCSK9-specific antibody responses were measured by coating ELISA plates with bovine serum albumin-PCSK9 conjugate or recombinant mouse PCSK9 (Abcam plc, Cambridge, UK, ab-167759). .. Candidate PCSK9 peptides were conjugated with bovine serum albumin at its N-terminus via suberic acid bis (PEPTIDE INSTITUTE Inc., Osaka, Japan).

    Immunostaining:

    Article Title: Sphingosine Kinase 1 Is Required for Mesothelioma Cell Proliferation: Role of Histone Acetylation
    Article Snippet: .. Non specific binding was blocked with BSA and then subjected to immunostaining using anti-SphK1 antibody (rabbit polyclonal; Abcam, Cambridge, UK), followed by goat anti- anti-rabbit IgG conjugated to a horseradish peroxidase–labeled polymer (Envision+ System, DAKO, Carpinteria, CA). .. The visualization was carried out using diaminobenzidine chromogen (DAKO), and then counterstained with hematoxylin.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Development of vaccine for dyslipidemia targeted to a proprotein convertase subtilisin/kexin type 9 (PCSK9) epitope in mice
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) PCSK9-specific antibody responses were measured by coating ELISA plates with bovine serum albumin-PCSK9 conjugate or recombinant mouse PCSK9 (Abcam plc, Cambridge, UK, ab-167759). .. Candidate PCSK9 peptides were conjugated with bovine serum albumin at its N-terminus via suberic acid bis (PEPTIDE INSTITUTE Inc., Osaka, Japan).

    Article Title: Extracellular matrix of secondary lymphoid organs impacts on B-cell fate and survival
    Article Snippet: .. Ninety-six-well ELISA plates were coated overnight at 4 °C with BSA, agrin, laminin 411, laminin 511, or fibronectin (3 µg/mL) and blocked with 2% (wt/vol) BSA, and BAFF (R & D 500 ng/mL) was added at room temperature for 2 h. Rat anti-BAFF (Abcam, 1 µg/mL) and HRP-conjugated goat anti-rat were used to detect bound BAFF. .. Mice were immunized by i.v. or i.p. injections of 50 μg NP-Ficoll in a volume of 200 μL.

    Incubation:

    Article Title: Combined effects of physiologically relevant disturbed wall shear stress and glycated albumin on endothelial cell functions associated with inflammation, thrombosis and cytoskeletal dynamics
    Article Snippet: .. Cells were washed with PBS‐0.1% BSA and incubated with 0.6 μg/mL anti‐human connexin‐43 (Abcam) and 2 μg/mL anti‐human caveolin‐1 (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C. .. The cells were again washed and incubated for 60 min with appropriate secondary antibodies (5 μg/mL; Invitrogen).

    Article Title: TLR9 and RIG-I Signaling in Human Endocervical Epithelial Cells Modulates Inflammatory Responses of Macrophages and Dendritic Cells In Vitro
    Article Snippet: .. The fixed cells were then blocked in 2% BSA in PBS for 1 h at room temperature followed by incubation overnight at 4°C with mouse monoclonal anti-TLR9 or rabbit polyclonal anti-RIG-I antibody (1∶100) or rabbit polyclonal p65 NF-kB (Ser536) antibody (1∶100 dilution) (Abcam). .. After washing with PBS, slides were incubated for 1 h at RT in FITC-labeled secondary antibody (1∶500 dilutions).

    Article Title: Apolipoprotein E binds to and reduces serum levels of DNA-mimicking, pyrrolated proteins
    Article Snippet: .. The cells were then sequentially incubated in PBS solutions containing 1% BSA (for 1 h) and anti-apoE mAb E6D7 (Abcam) containing 0.1% BSA (overnight, 4 °C). .. The cells were treated with Alexa Fluor® 488–conjugated anti-mouse IgG (Invitrogen) for 1 h, rinsed with PBS, and covered with anti-fade solution (Invitrogen).

    Blocking Assay:

    Article Title: The endoplasmic reticulum–resident collagen chaperone Hsp47 interacts with and promotes the secretion of decorin, fibromodulin, and lumican
    Article Snippet: .. Immunoblots for Hsp47 were performed by blocking the membrane in 5% (w/v) BSA in TBS for 1 h and then incubating with anti-Hsp47 (Abcam 109117) diluted 1:1,000 in 5% (w/v) BSA in TBST, washing three times for 5 min each with TBST, incubating with HRP-conjugated Protein A (Abcam) diluted 1:5,000 in 5% (w/v) BSA in TBST, washing three times for 5 min each in TBST, and developing the membrane using Luminata Forte (Millipore). .. Recombinant Hsp47 or BSA was covalently coupled to magnetic beads using Dynabeads according to the manufacturer's instructions (Thermo Fisher Scientific) and blocked with 1% (w/v) BSA.

    Article Title: Alendronate-induced disruption of actin cytoskeleton and inhibition of migration/invasion are associated with cofilin downregulation in PC-3 prostate cancer cells
    Article Snippet: .. Immunocytochemical cofilin staining was performed in 3% PFA-fixed cells by blocking with 0.1% BSA in PBS for 1 hour and then incubating with anti-cofilin antibody (Abcam, USA) for an additional hour. .. After PBS washings, cells were stained with chicken anti-rabbit IgG (H+L) Alexa Fluor® 488 (Invitrogen, USA) and TRITC-phalloidin (0.2 μg/ml) (Sigma, USA) for 1 hour.

    Western Blot:

    Article Title: The endoplasmic reticulum–resident collagen chaperone Hsp47 interacts with and promotes the secretion of decorin, fibromodulin, and lumican
    Article Snippet: .. Immunoblots for Hsp47 were performed by blocking the membrane in 5% (w/v) BSA in TBS for 1 h and then incubating with anti-Hsp47 (Abcam 109117) diluted 1:1,000 in 5% (w/v) BSA in TBST, washing three times for 5 min each with TBST, incubating with HRP-conjugated Protein A (Abcam) diluted 1:5,000 in 5% (w/v) BSA in TBST, washing three times for 5 min each in TBST, and developing the membrane using Luminata Forte (Millipore). .. Recombinant Hsp47 or BSA was covalently coupled to magnetic beads using Dynabeads according to the manufacturer's instructions (Thermo Fisher Scientific) and blocked with 1% (w/v) BSA.

    Staining:

    Article Title: Alendronate-induced disruption of actin cytoskeleton and inhibition of migration/invasion are associated with cofilin downregulation in PC-3 prostate cancer cells
    Article Snippet: .. Immunocytochemical cofilin staining was performed in 3% PFA-fixed cells by blocking with 0.1% BSA in PBS for 1 hour and then incubating with anti-cofilin antibody (Abcam, USA) for an additional hour. .. After PBS washings, cells were stained with chicken anti-rabbit IgG (H+L) Alexa Fluor® 488 (Invitrogen, USA) and TRITC-phalloidin (0.2 μg/ml) (Sigma, USA) for 1 hour.

    Binding Assay:

    Article Title: Sphingosine Kinase 1 Is Required for Mesothelioma Cell Proliferation: Role of Histone Acetylation
    Article Snippet: .. Non specific binding was blocked with BSA and then subjected to immunostaining using anti-SphK1 antibody (rabbit polyclonal; Abcam, Cambridge, UK), followed by goat anti- anti-rabbit IgG conjugated to a horseradish peroxidase–labeled polymer (Envision+ System, DAKO, Carpinteria, CA). .. The visualization was carried out using diaminobenzidine chromogen (DAKO), and then counterstained with hematoxylin.

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