fatty acid depleted bovine serum albumin bsa  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Bovine Serum Albumin Fraction V
    Description:
    Highest quality for fatty acid and lipid research BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates At pH 5 7 it contains 17 intra chain disulfide bridges and one sulfhydryl group
    Catalog Number:
    10775835001
    Price:
    None
    Applications:
    Bovine Serum Albumin, Fraction V, fatty acid free is used:. in lipid research to study lipid metabolism in biological systems. to identify transport mechanisms in living organisms. to study ligand binding. to investigate ionic binding. in the preparation of solutions during isolation of cardiomyocytes. as a blocking solution to incubate embryos for immunofluorescence. in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers
    Buy from Supplier


    Structured Review

    Millipore fatty acid depleted bovine serum albumin bsa
    Highest quality for fatty acid and lipid research BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates At pH 5 7 it contains 17 intra chain disulfide bridges and one sulfhydryl group
    https://www.bioz.com/result/fatty acid depleted bovine serum albumin bsa/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fatty acid depleted bovine serum albumin bsa - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Fasting‐induced liver GADD45β restrains hepatic fatty acid uptake and improves metabolic health
    Article Snippet: .. In addition, liver slices from the same mice were allowed to incubate in media described above without glucose but with (BSA‐NEFA) or without (fatty acid‐free BSA vehicle) for 18 h and glucose concentration of the media was measured (GAHK20, Sigma‐Aldrich, DEU) and subsequently glucose production rate was calculated. .. Plasmids, RNA interference and recombinant viruses AAV encoding control or specific miRNAs under the control of a hepatocyte‐specific promoter were established, purified and tittered as described previously (Graham et al , ; Rose et al , ).

    Mouse Assay:

    Article Title: Fasting‐induced liver GADD45β restrains hepatic fatty acid uptake and improves metabolic health
    Article Snippet: .. In addition, liver slices from the same mice were allowed to incubate in media described above without glucose but with (BSA‐NEFA) or without (fatty acid‐free BSA vehicle) for 18 h and glucose concentration of the media was measured (GAHK20, Sigma‐Aldrich, DEU) and subsequently glucose production rate was calculated. .. Plasmids, RNA interference and recombinant viruses AAV encoding control or specific miRNAs under the control of a hepatocyte‐specific promoter were established, purified and tittered as described previously (Graham et al , ; Rose et al , ).

    Immunocytochemistry:

    Article Title: Kdo2-Lipid A, a TLR4-specific Agonist, Induces de Novo Sphingolipid Biosynthesis in RAW264.7 Macrophages, Which Is Essential for Induction of Autophagy *
    Article Snippet: .. Materials The suppliers for the reagents were as follows: Kdo2 -lipid A and the internal standard mixture for SL analysis by LC ESI-MS/MS (ceramide/sphingoid internal standard mixture II, LM-6005) (Avanti Polar Lipids, Alabaster, AL); [U-13 C]palmitic acid (98%) (Cambridge Isotopes, Andover, MA); fatty acid-free BSA (Calbiochem) ; Hoechst 33342 (Invitrogen); ISP1 (Biomol, Plymouth Meeting, PA); mouse monoclonal antibody to SPT1 (anti-LCB1), anti-BiP/GRP78, and anti-GM130 (BD Biosciences); rabbit monoclonal anti-HA tag and rabbit polyclonal LC3B antibody (Cell Signaling Technology, Boston); mouse monoclonal anti-Cer antibody clone 15B4 (Alexis Biochemicals, San Diego) This antibody clone has been successfully used in immunocytochemistry applications with no indication of nonspecific reactions with other lipids ( ) The secondary Alexa Fluor-conjugated F(ab)2 fragment goat anti-mouse antibody and goat anti-rabbit antibody were from Molecular Probes, Inc. (Eugene, OR.). .. The sources for liquid chromatography (LC) columns, solvents, and other reagents used for mass spectrometry were the same as in the published method ( ).

    Cell Culture:

    Article Title: A GPX4-dependent cancer cell state underlies the clear-cell morphology and confers sensitivity to ferroptosis
    Article Snippet: .. Polyunsaturated acids (arachidonic acid, C20:4) (Cayman Chemicals) were conjugated with fatty-acid free BSA (Sigma-Aldrich) using previously described protocols , and were applied to cell culture media at 20 μM for 3 days. .. Gene-expression analysis by qRT-PCR Total RNA was extracted from cells using RNeasy Mini kit (Qiagen) following the manufacturer’s instructions. cDNA was synthesized using the ProtoScript First Strand cDNA Synthesis kit (New England Biolabs).

    Purification:

    Article Title: Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells *Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells * s⃞
    Article Snippet: .. Several sets of experiments were performed with the ligand present in the syringe (300 μl at 5 mM) or in the cell (1.6 ml at 12.5 μM) with either purified ACBD6 or fatty acid-free BSA (Sigma) in the cell at 25 μM or in the syringe at 125 μM, respectively. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore fatty acid free bsa
    Analysis of de novo sphingolipid biosynthesis in RAW264.7 cells by [ 13 C]palmitate labeling. RAW264.7 cells were incubated with 0.1 m m [U- 13 C]palmitic acid (as the <t>BSA</t> complex) with vehicle control (PBS), KLA (100 ng/ml), <t>ISP1</t> (1 μ m ), or KLA+ ISP1. For cells treated with KLA + ISP1, ISP1 was added 1 h prior to the addition of KLA for the times shown. The appearance of 13 C in newly synthesized sphingolipids was quantified by mass spectrometry as described under supplemental “Materials and Methods” . A , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer subspecies. Data represent the means ± S.E. ( n = 3). B , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer species in cells treated with control ( left ) or KLA ( right ), Data represent the means ( n = 3). Because of the difficulties in visualization, the quantities of ceramide subspecies in control treated cells at the 24-h time point are as follows: C16 (7.6 pmol/μg DNA); C18 (0.7 pmol/μg DNA); C20 (0.3 pmol/μg DNA); C22 (3.2 pmol/μg DNA); C24:1 (6 pmol/μg DNA); C24 (6.4 pmol/μg DNA); C26:1 (0.2 pmol/μg DNA); C26 (0.1 pmol/μg DNA). C , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer species ( left ) and 12 C (unlabeled) Cer species ( right ) in cells treated with KLA + ISP1. Data represent the means ( n = 3).
    Fatty Acid Free Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fatty acid free bsa/product/Millipore
    Average 99 stars, based on 509 article reviews
    Price from $9.99 to $1999.99
    fatty acid free bsa - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore ffa bsa
    Effects of OF and CM on the mRNA expressions of lipogenesis-related genes. Real-time RT-PCR analysis of sterol regulatory element-binding protein-1 ( SREBP-1c ) (a) and glycerol-3-phosphate acyltransferase ( GPAT ) (b) mRNA levels in 1 mM <t>FFA/BSA-treated</t> HepG2 cells. All data were normalized to GAPDH mRNA, and the fold changes in expression were calculated relative to control cells (treated with 1% BSA). Each experiment was independently performed three times. Data were presented as the mean ± SD. Values not sharing common superscripts are significantly different ( p
    Ffa Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ffa bsa/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ffa bsa - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of de novo sphingolipid biosynthesis in RAW264.7 cells by [ 13 C]palmitate labeling. RAW264.7 cells were incubated with 0.1 m m [U- 13 C]palmitic acid (as the BSA complex) with vehicle control (PBS), KLA (100 ng/ml), ISP1 (1 μ m ), or KLA+ ISP1. For cells treated with KLA + ISP1, ISP1 was added 1 h prior to the addition of KLA for the times shown. The appearance of 13 C in newly synthesized sphingolipids was quantified by mass spectrometry as described under supplemental “Materials and Methods” . A , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer subspecies. Data represent the means ± S.E. ( n = 3). B , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer species in cells treated with control ( left ) or KLA ( right ), Data represent the means ( n = 3). Because of the difficulties in visualization, the quantities of ceramide subspecies in control treated cells at the 24-h time point are as follows: C16 (7.6 pmol/μg DNA); C18 (0.7 pmol/μg DNA); C20 (0.3 pmol/μg DNA); C22 (3.2 pmol/μg DNA); C24:1 (6 pmol/μg DNA); C24 (6.4 pmol/μg DNA); C26:1 (0.2 pmol/μg DNA); C26 (0.1 pmol/μg DNA). C , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer species ( left ) and 12 C (unlabeled) Cer species ( right ) in cells treated with KLA + ISP1. Data represent the means ( n = 3).

    Journal: The Journal of Biological Chemistry

    Article Title: Kdo2-Lipid A, a TLR4-specific Agonist, Induces de Novo Sphingolipid Biosynthesis in RAW264.7 Macrophages, Which Is Essential for Induction of Autophagy *

    doi: 10.1074/jbc.M110.170621

    Figure Lengend Snippet: Analysis of de novo sphingolipid biosynthesis in RAW264.7 cells by [ 13 C]palmitate labeling. RAW264.7 cells were incubated with 0.1 m m [U- 13 C]palmitic acid (as the BSA complex) with vehicle control (PBS), KLA (100 ng/ml), ISP1 (1 μ m ), or KLA+ ISP1. For cells treated with KLA + ISP1, ISP1 was added 1 h prior to the addition of KLA for the times shown. The appearance of 13 C in newly synthesized sphingolipids was quantified by mass spectrometry as described under supplemental “Materials and Methods” . A , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer subspecies. Data represent the means ± S.E. ( n = 3). B , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer species in cells treated with control ( left ) or KLA ( right ), Data represent the means ( n = 3). Because of the difficulties in visualization, the quantities of ceramide subspecies in control treated cells at the 24-h time point are as follows: C16 (7.6 pmol/μg DNA); C18 (0.7 pmol/μg DNA); C20 (0.3 pmol/μg DNA); C22 (3.2 pmol/μg DNA); C24:1 (6 pmol/μg DNA); C24 (6.4 pmol/μg DNA); C26:1 (0.2 pmol/μg DNA); C26 (0.1 pmol/μg DNA). C , summation of base (backbone)-labeled and dual (backbone and fatty acid)-labeled Cer species ( left ) and 12 C (unlabeled) Cer species ( right ) in cells treated with KLA + ISP1. Data represent the means ( n = 3).

    Article Snippet: Materials The suppliers for the reagents were as follows: Kdo2 -lipid A and the internal standard mixture for SL analysis by LC ESI-MS/MS (ceramide/sphingoid internal standard mixture II, LM-6005) (Avanti Polar Lipids, Alabaster, AL); [U-13 C]palmitic acid (98%) (Cambridge Isotopes, Andover, MA); fatty acid-free BSA (Calbiochem) ; Hoechst 33342 (Invitrogen); ISP1 (Biomol, Plymouth Meeting, PA); mouse monoclonal antibody to SPT1 (anti-LCB1), anti-BiP/GRP78, and anti-GM130 (BD Biosciences); rabbit monoclonal anti-HA tag and rabbit polyclonal LC3B antibody (Cell Signaling Technology, Boston); mouse monoclonal anti-Cer antibody clone 15B4 (Alexis Biochemicals, San Diego) This antibody clone has been successfully used in immunocytochemistry applications with no indication of nonspecific reactions with other lipids ( ) The secondary Alexa Fluor-conjugated F(ab)2 fragment goat anti-mouse antibody and goat anti-rabbit antibody were from Molecular Probes, Inc. (Eugene, OR.).

    Techniques: Labeling, Incubation, Synthesized, Mass Spectrometry

    PA-induced lipotoxicity and DHA neuroprotective action on primary rat cortical cells (RCC). A : Cellular morphology of control and treated (PA/BSA, 2:1, 48 hr) RCC. B : Nuclear morphology of control and treated RCC. Arrows indicate fragmented nuclei. C : Viability of RCC treated for 48 hr with PA/BSA (2:1 ratio; PA) or in cotreatment with DHA (PA + DHA). Viability was determined using the WST-1 assay. Data represent mean ± SEM of three independent experiments. ★ P

    Journal: Journal of neuroscience research

    Article Title: Activation and Reversal of Lipotoxicity in PC12 and Rat Cortical Cells Following Exposure to Palmitic Acid

    doi: 10.1002/jnr.21918

    Figure Lengend Snippet: PA-induced lipotoxicity and DHA neuroprotective action on primary rat cortical cells (RCC). A : Cellular morphology of control and treated (PA/BSA, 2:1, 48 hr) RCC. B : Nuclear morphology of control and treated RCC. Arrows indicate fragmented nuclei. C : Viability of RCC treated for 48 hr with PA/BSA (2:1 ratio; PA) or in cotreatment with DHA (PA + DHA). Viability was determined using the WST-1 assay. Data represent mean ± SEM of three independent experiments. ★ P

    Article Snippet: PA and/or DHA (Sigma, St. Louis, MO) was first dissolved/diluted in 100% ethanol and further diluted in warm low-serum medium for NGFDPC12 cells, or serum-free medium for RCC, with fatty acid-free BSA (EMD Biosciences, La Jolla, CA).

    Techniques: WST-1 Assay

    Palmitic acid-induced lipotoxicity in NGFDPC12 cells. A : NGFDPC12 cells were exposed to PA/BSA (2:1 ratio), and viability was determined using WST-1 assay at the indicated times. B : NGFDPC12 cells were exposed to different PA/BSA ratios (0.25:1, 0.5:1, 1:1, and 2:1 with BSA at a concentration of 0.150 mM) for 24 hr. Cell viability was measured by the WST-1 assay. C : NGFDPC12 cells were exposed to 0.150 mM BSA without PA (control) or PA/BSA (2:1; PA) for 12 hr. Cells were stained with Hoechst (10 ng/liter), and nuclei were visualized under fluorescent microscopy. Arrows indicate nuclei showing chromatin condensation and fragmentation. D : Regulation of apoptosis-asociated genes in NGFDPC12 cultures exposed to PA/BSA (2:1). Quantitative RT-PCR experiments show the time-dependent mRNA expression of Bim, Bad, AIF, BNIP-3, 14.3.3, and FAS-R. GAPDH mRNA expression was used as the internal control. Data represent mean 6 SEM of three independent experiments. ★ P

    Journal: Journal of neuroscience research

    Article Title: Activation and Reversal of Lipotoxicity in PC12 and Rat Cortical Cells Following Exposure to Palmitic Acid

    doi: 10.1002/jnr.21918

    Figure Lengend Snippet: Palmitic acid-induced lipotoxicity in NGFDPC12 cells. A : NGFDPC12 cells were exposed to PA/BSA (2:1 ratio), and viability was determined using WST-1 assay at the indicated times. B : NGFDPC12 cells were exposed to different PA/BSA ratios (0.25:1, 0.5:1, 1:1, and 2:1 with BSA at a concentration of 0.150 mM) for 24 hr. Cell viability was measured by the WST-1 assay. C : NGFDPC12 cells were exposed to 0.150 mM BSA without PA (control) or PA/BSA (2:1; PA) for 12 hr. Cells were stained with Hoechst (10 ng/liter), and nuclei were visualized under fluorescent microscopy. Arrows indicate nuclei showing chromatin condensation and fragmentation. D : Regulation of apoptosis-asociated genes in NGFDPC12 cultures exposed to PA/BSA (2:1). Quantitative RT-PCR experiments show the time-dependent mRNA expression of Bim, Bad, AIF, BNIP-3, 14.3.3, and FAS-R. GAPDH mRNA expression was used as the internal control. Data represent mean 6 SEM of three independent experiments. ★ P

    Article Snippet: PA and/or DHA (Sigma, St. Louis, MO) was first dissolved/diluted in 100% ethanol and further diluted in warm low-serum medium for NGFDPC12 cells, or serum-free medium for RCC, with fatty acid-free BSA (EMD Biosciences, La Jolla, CA).

    Techniques: WST-1 Assay, Concentration Assay, Staining, Microscopy, Quantitative RT-PCR, Expressing

    DNA fragmentation and caspase-3 activity of NGFDPC12 cells exposed to PA. A : Representative experimental results of BRDU-FITC plots (apoptotic cells) vs. PI (total DNA marker) for controls and cells exposed to PA/BSA (2:1) for 12 hr. The R2 quadrant shows cells exhibiting increased BrdU-FITC fluorescence (green). Nonapoptotic cells are indicated in red. B : Representative distribution diagram of positive apoptotic NGFDPC12 cells. Cells were treated with BSA for negative control (green), PA/BSA (2:1) for 12 hr (red) or 24 hr (blue), or staurosporine for positive control (orange). C : Quantitative analysis of positive apoptotic NGFDPC12 cells as shown in B. The data represent the mean ± SEM of three independent experiments done in triplicate. D : Caspase-3 activity in cell extracts was determined in control or NGFDPC12 cells treated with PA/BSA for 12 hr. E : Early posttreatment with BSA protects NGFDPC12 cells from PA-induced lipotoxicity. Additional BSA (0.6 mM final concentration) was added to NGFDPC-12 cells at 2 or 6 hr after initial PA/BSA (2:1 ratio) treatment. Percentage of viable cells was measured by the WST-1 assay at the end of a 24-hr incubation period. Nuclei in bottom panel were visualized with Hoechst staining. Data represent mean ± SEM of three independent experiments. ★ P

    Journal: Journal of neuroscience research

    Article Title: Activation and Reversal of Lipotoxicity in PC12 and Rat Cortical Cells Following Exposure to Palmitic Acid

    doi: 10.1002/jnr.21918

    Figure Lengend Snippet: DNA fragmentation and caspase-3 activity of NGFDPC12 cells exposed to PA. A : Representative experimental results of BRDU-FITC plots (apoptotic cells) vs. PI (total DNA marker) for controls and cells exposed to PA/BSA (2:1) for 12 hr. The R2 quadrant shows cells exhibiting increased BrdU-FITC fluorescence (green). Nonapoptotic cells are indicated in red. B : Representative distribution diagram of positive apoptotic NGFDPC12 cells. Cells were treated with BSA for negative control (green), PA/BSA (2:1) for 12 hr (red) or 24 hr (blue), or staurosporine for positive control (orange). C : Quantitative analysis of positive apoptotic NGFDPC12 cells as shown in B. The data represent the mean ± SEM of three independent experiments done in triplicate. D : Caspase-3 activity in cell extracts was determined in control or NGFDPC12 cells treated with PA/BSA for 12 hr. E : Early posttreatment with BSA protects NGFDPC12 cells from PA-induced lipotoxicity. Additional BSA (0.6 mM final concentration) was added to NGFDPC-12 cells at 2 or 6 hr after initial PA/BSA (2:1 ratio) treatment. Percentage of viable cells was measured by the WST-1 assay at the end of a 24-hr incubation period. Nuclei in bottom panel were visualized with Hoechst staining. Data represent mean ± SEM of three independent experiments. ★ P

    Article Snippet: PA and/or DHA (Sigma, St. Louis, MO) was first dissolved/diluted in 100% ethanol and further diluted in warm low-serum medium for NGFDPC12 cells, or serum-free medium for RCC, with fatty acid-free BSA (EMD Biosciences, La Jolla, CA).

    Techniques: Activity Assay, Marker, Fluorescence, Negative Control, Positive Control, Concentration Assay, WST-1 Assay, Incubation, Staining

    ROS generation and changes in mitochondrial membrane permeability in NGFDPC12 cells undergoing PA-induced lipotoxicity. A : ROS generation in NGFDPC12 cells was detected by the 2′,7′ -dichlorofluorescein diacetate (DCF) method at 12 hr after the initial exposure to PA using flow cytometry. NGFDPC12 cells were treated with no DCF (light green), DCF (dark green), PA + DCF (blue), or hydrogen peroxide + DCF (red). B : Quantification of ROS production in NGFDPC12 cells at 3, 6, 9, and 12 hr after PA/BSA treatment. C : ROS modulation after addition of DHA, MCI-186, or BSA to NCGDPC12 cells treated with PA for 6 hr. D: Mitochondrial depolarization in NGFDPC12 cells, control or PA/BSA treated, detected by JC-1 flow cytometric analysis. JC-1 was detected in the FL1 or FL2 channel depending on its aggregation status (see Mqaterials and Methods). The R2 quadrant defines cells with leaky mitochondria showing reduced FL2 readings (green). Cells with intact mitochondria are indicated in R1 (red). Data represent mean ± SEM sent of at least three independent experiments. ★ P

    Journal: Journal of neuroscience research

    Article Title: Activation and Reversal of Lipotoxicity in PC12 and Rat Cortical Cells Following Exposure to Palmitic Acid

    doi: 10.1002/jnr.21918

    Figure Lengend Snippet: ROS generation and changes in mitochondrial membrane permeability in NGFDPC12 cells undergoing PA-induced lipotoxicity. A : ROS generation in NGFDPC12 cells was detected by the 2′,7′ -dichlorofluorescein diacetate (DCF) method at 12 hr after the initial exposure to PA using flow cytometry. NGFDPC12 cells were treated with no DCF (light green), DCF (dark green), PA + DCF (blue), or hydrogen peroxide + DCF (red). B : Quantification of ROS production in NGFDPC12 cells at 3, 6, 9, and 12 hr after PA/BSA treatment. C : ROS modulation after addition of DHA, MCI-186, or BSA to NCGDPC12 cells treated with PA for 6 hr. D: Mitochondrial depolarization in NGFDPC12 cells, control or PA/BSA treated, detected by JC-1 flow cytometric analysis. JC-1 was detected in the FL1 or FL2 channel depending on its aggregation status (see Mqaterials and Methods). The R2 quadrant defines cells with leaky mitochondria showing reduced FL2 readings (green). Cells with intact mitochondria are indicated in R1 (red). Data represent mean ± SEM sent of at least three independent experiments. ★ P

    Article Snippet: PA and/or DHA (Sigma, St. Louis, MO) was first dissolved/diluted in 100% ethanol and further diluted in warm low-serum medium for NGFDPC12 cells, or serum-free medium for RCC, with fatty acid-free BSA (EMD Biosciences, La Jolla, CA).

    Techniques: Permeability, Flow Cytometry, Cytometry

    Inhibition of fatty acid-induced lipotoxicity by DHA. A : DHA inhibits induction of cell death by PA in NGFDPC12 cells. Cell viability was determined after treatment for 24 hr as follows: 0.150 mM BSA alone (control), PA/BSA 2:1 ratio (PA), PA/BSA 2:1 cotreated with DHA followed by an increase in BSA final concentration to 0.6 mM at 6 hr (PA + DHA + BSA at 6 hr), and DHA pretreatment for 12 hr followed by treatment with PA/BSA 2:1 (DHA 12 hr pre + PA). B : DHA treatment inhibits apoptotic features of NGFDPC12 cells treated with PA/BSA. Morphology of NGFDPC12 cells treated with BSA alone (control), PA/BSA 2:1 cotreated with DHA, or PA/BSA 2:1 for 24 hr. C : Nuclear morphology of NGFDPC12 cells treated with BSA alone (control), PA/BSA 2:1 cotreated with DHA or PA/BSA 2:1 for 24 hr. Arrows indicate nuclei exhibiting fragmentation. D : DHA cotreatment produced a significant inhibitory effect of caspase-3 activity in cellular extracts of NGFDPC12 cells exposed to PA/BSA (2:1). E : Cleavage of PARP and lamin B in NGFDPC-12 cells exposed to PA/BSA (2:1), as assessed by Western blotting. PA treatment induced the appearance of the signature apoptotic fragments of PARP (85 kDa) or lamin B (46 kDa). DHA inhibited PARP and lamin B cleavage. Data in A and D represent mean ± SEM of three independent experiments. ★ P

    Journal: Journal of neuroscience research

    Article Title: Activation and Reversal of Lipotoxicity in PC12 and Rat Cortical Cells Following Exposure to Palmitic Acid

    doi: 10.1002/jnr.21918

    Figure Lengend Snippet: Inhibition of fatty acid-induced lipotoxicity by DHA. A : DHA inhibits induction of cell death by PA in NGFDPC12 cells. Cell viability was determined after treatment for 24 hr as follows: 0.150 mM BSA alone (control), PA/BSA 2:1 ratio (PA), PA/BSA 2:1 cotreated with DHA followed by an increase in BSA final concentration to 0.6 mM at 6 hr (PA + DHA + BSA at 6 hr), and DHA pretreatment for 12 hr followed by treatment with PA/BSA 2:1 (DHA 12 hr pre + PA). B : DHA treatment inhibits apoptotic features of NGFDPC12 cells treated with PA/BSA. Morphology of NGFDPC12 cells treated with BSA alone (control), PA/BSA 2:1 cotreated with DHA, or PA/BSA 2:1 for 24 hr. C : Nuclear morphology of NGFDPC12 cells treated with BSA alone (control), PA/BSA 2:1 cotreated with DHA or PA/BSA 2:1 for 24 hr. Arrows indicate nuclei exhibiting fragmentation. D : DHA cotreatment produced a significant inhibitory effect of caspase-3 activity in cellular extracts of NGFDPC12 cells exposed to PA/BSA (2:1). E : Cleavage of PARP and lamin B in NGFDPC-12 cells exposed to PA/BSA (2:1), as assessed by Western blotting. PA treatment induced the appearance of the signature apoptotic fragments of PARP (85 kDa) or lamin B (46 kDa). DHA inhibited PARP and lamin B cleavage. Data in A and D represent mean ± SEM of three independent experiments. ★ P

    Article Snippet: PA and/or DHA (Sigma, St. Louis, MO) was first dissolved/diluted in 100% ethanol and further diluted in warm low-serum medium for NGFDPC12 cells, or serum-free medium for RCC, with fatty acid-free BSA (EMD Biosciences, La Jolla, CA).

    Techniques: Inhibition, Concentration Assay, Produced, Activity Assay, Western Blot

    DHA reduces mitochondrial depolarization of NGFDPC12 cells undergoing PA-induced lipotoxicity. A : NGFDPC12 cells were treated with PA/BSA alone (PA) or in the presence DHA (PA + DHA) for 9 or 12 hr, and mitochondrial depolarization was determined by JC-1 flow cytometry assays. Representative flow cytometric plots are shown. B : Quantification of mitochondria depolarization (R2 in JC-1 flow cytometry) in NGFDPC12 cells undergoing PA-induced lipotoxicity. Data represent mean ± SEM of three independent experiments. ★ P

    Journal: Journal of neuroscience research

    Article Title: Activation and Reversal of Lipotoxicity in PC12 and Rat Cortical Cells Following Exposure to Palmitic Acid

    doi: 10.1002/jnr.21918

    Figure Lengend Snippet: DHA reduces mitochondrial depolarization of NGFDPC12 cells undergoing PA-induced lipotoxicity. A : NGFDPC12 cells were treated with PA/BSA alone (PA) or in the presence DHA (PA + DHA) for 9 or 12 hr, and mitochondrial depolarization was determined by JC-1 flow cytometry assays. Representative flow cytometric plots are shown. B : Quantification of mitochondria depolarization (R2 in JC-1 flow cytometry) in NGFDPC12 cells undergoing PA-induced lipotoxicity. Data represent mean ± SEM of three independent experiments. ★ P

    Article Snippet: PA and/or DHA (Sigma, St. Louis, MO) was first dissolved/diluted in 100% ethanol and further diluted in warm low-serum medium for NGFDPC12 cells, or serum-free medium for RCC, with fatty acid-free BSA (EMD Biosciences, La Jolla, CA).

    Techniques: Flow Cytometry, Cytometry

    Anti-glycan IgG in the sera of lambs vaccinated with Haemonchus contortus excretory/secretory (ES) products analyzed by ELISA. The amount of IgG against Galα1-3GalNAc-polyacrylamide (PAA), GalNAcβ1-4(Fucα1-3)GlcNAc-BSA (LDNF) and

    Journal: International journal for parasitology

    Article Title: Vaccination-induced IgG response to Gal?1-3GalNAc glycan epitopes in lambs protected against Haemonchus contortus challenge infection

    doi: 10.1016/j.ijpara.2009.07.009

    Figure Lengend Snippet: Anti-glycan IgG in the sera of lambs vaccinated with Haemonchus contortus excretory/secretory (ES) products analyzed by ELISA. The amount of IgG against Galα1-3GalNAc-polyacrylamide (PAA), GalNAcβ1-4(Fucα1-3)GlcNAc-BSA (LDNF) and

    Article Snippet: After blocking (60 min 37°C) with 1% ELISA-grade BSA (Fraction V, fatty acid free; Calbiochem, San Diego, USA) in TSM (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 2 mM CaCl2 , 2 mM MgCl2 ) and washing with TSM containing 0.1% Tween-20, glycan-specific antibodies or biotinylated GSI-B4 were added for 60 min at 37°C.

    Techniques: Enzyme-linked Immunosorbent Assay

    Effects of OF and CM on the mRNA expressions of lipogenesis-related genes. Real-time RT-PCR analysis of sterol regulatory element-binding protein-1 ( SREBP-1c ) (a) and glycerol-3-phosphate acyltransferase ( GPAT ) (b) mRNA levels in 1 mM FFA/BSA-treated HepG2 cells. All data were normalized to GAPDH mRNA, and the fold changes in expression were calculated relative to control cells (treated with 1% BSA). Each experiment was independently performed three times. Data were presented as the mean ± SD. Values not sharing common superscripts are significantly different ( p

    Journal: Mediators of Inflammation

    Article Title: Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells

    doi: 10.1155/2017/4856095

    Figure Lengend Snippet: Effects of OF and CM on the mRNA expressions of lipogenesis-related genes. Real-time RT-PCR analysis of sterol regulatory element-binding protein-1 ( SREBP-1c ) (a) and glycerol-3-phosphate acyltransferase ( GPAT ) (b) mRNA levels in 1 mM FFA/BSA-treated HepG2 cells. All data were normalized to GAPDH mRNA, and the fold changes in expression were calculated relative to control cells (treated with 1% BSA). Each experiment was independently performed three times. Data were presented as the mean ± SD. Values not sharing common superscripts are significantly different ( p

    Article Snippet: The FFA/BSA or oleic acid (OA)/BSA complex solution was sterile-filtered through 0.22 μ m sterile filters (Millipore S.A.S., Molsheim, France) and then stored at −20°C until use.

    Techniques: Quantitative RT-PCR, Binding Assay, Expressing

    Effect of OF and CM on FFA-induced ROS production. HepG2 cells were incubated with 1 mM FFAs/BSA for 24 h in the presence of OF or CM. Vehicle cells were incubated with 0.1% DMSO in the presence of FFAs/BSA. Control cells were incubated with 1% BSA. Intracellular ROS production was quantified using the fluorescent probe DCFDA. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ( p

    Journal: Mediators of Inflammation

    Article Title: Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells

    doi: 10.1155/2017/4856095

    Figure Lengend Snippet: Effect of OF and CM on FFA-induced ROS production. HepG2 cells were incubated with 1 mM FFAs/BSA for 24 h in the presence of OF or CM. Vehicle cells were incubated with 0.1% DMSO in the presence of FFAs/BSA. Control cells were incubated with 1% BSA. Intracellular ROS production was quantified using the fluorescent probe DCFDA. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ( p

    Article Snippet: The FFA/BSA or oleic acid (OA)/BSA complex solution was sterile-filtered through 0.22 μ m sterile filters (Millipore S.A.S., Molsheim, France) and then stored at −20°C until use.

    Techniques: Incubation

    Effects of OF and CM on lipid accumulation in free fatty acid-overloaded HepG2 cells. HepG2 cells were incubated with 1 mM FFAs/BSA and cotreated with various concentrations of OF and CM for 24 h. Vehicle cells were incubated with 0.1% DMSO in the presence of FFAs/BSA. Control cells were incubated with 1% BSA. Cell viability was measured by the MTT assay (a). Quantitative analysis of lipid deposition in cells by the OD 500 nm values using Oil Red O staining (b). Intracellular triglyceride (c) and cholesterol (d) contents were determined in cell lysates by an enzymatic colorimetric method using a commercially available kit. Total cholesterol and TG levels of the control cells were 25.3 ± 7.1 and 28.7 ± 2.7 μ g/mg of cellular protein, respectively. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ( p

    Journal: Mediators of Inflammation

    Article Title: Antilipotoxicity Activity of Osmanthus fragrans and Chrysanthemum morifolium Flower Extracts in Hepatocytes and Renal Glomerular Mesangial Cells

    doi: 10.1155/2017/4856095

    Figure Lengend Snippet: Effects of OF and CM on lipid accumulation in free fatty acid-overloaded HepG2 cells. HepG2 cells were incubated with 1 mM FFAs/BSA and cotreated with various concentrations of OF and CM for 24 h. Vehicle cells were incubated with 0.1% DMSO in the presence of FFAs/BSA. Control cells were incubated with 1% BSA. Cell viability was measured by the MTT assay (a). Quantitative analysis of lipid deposition in cells by the OD 500 nm values using Oil Red O staining (b). Intracellular triglyceride (c) and cholesterol (d) contents were determined in cell lysates by an enzymatic colorimetric method using a commercially available kit. Total cholesterol and TG levels of the control cells were 25.3 ± 7.1 and 28.7 ± 2.7 μ g/mg of cellular protein, respectively. Data were presented as mean ± SD of three independent experiments. Values not sharing common superscripts are significantly different ( p

    Article Snippet: The FFA/BSA or oleic acid (OA)/BSA complex solution was sterile-filtered through 0.22 μ m sterile filters (Millipore S.A.S., Molsheim, France) and then stored at −20°C until use.

    Techniques: Incubation, MTT Assay, Staining