fastdigest mspi  (Thermo Fisher)


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    Name:
    FastDigest MspI
    Description:
    5 C ↓C G G 3 3 G G C ↑C 5 Thermo Scientific FastDigest MspI restriction enzyme recognizes C CGG site and cuts best at 37°C in 5 15 minutes using universal FastDigest Buffer Isoschizomers BsiSI HapII Thermo Scientific FastDigest MspI is one of an advanced line of fast restriction enzymes that are all 100 active in the universal FastDigest and FastDigest Green reaction buffers The universal buffer allows rapid single double or multiple DNA digestion within 5 15 minutes eliminating any need for buffer change or subsequent DNA clean up steps See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity heat inactivation and incubation times for this and other FastDigest restriction enzymes DNA modifying enzymes such as Klenow Fragment T4 DNA Ligase alkaline phosphatases and T4 DNA Polymerase all have 100 activity in FastDigest Buffer Therefore enzymes for downstream applications can be directly added to the FastDigest reaction mix For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects Features• 100 activity of all FastDigest enzymes in the universal buffer• 100 buffer compatibility with downstream applications• Complete digestion in 5 15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism RFLP • SNP analysisNote The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer For applications that require product analysis by fluorescence excitation e g concentration measurements in UV light the colorless FastDigest Buffer is recommended For methylation sensitivity refer to product specifications
    Catalog Number:
    fd0544
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher fastdigest mspi
    5 C ↓C G G 3 3 G G C ↑C 5 Thermo Scientific FastDigest MspI restriction enzyme recognizes C CGG site and cuts best at 37°C in 5 15 minutes using universal FastDigest Buffer Isoschizomers BsiSI HapII Thermo Scientific FastDigest MspI is one of an advanced line of fast restriction enzymes that are all 100 active in the universal FastDigest and FastDigest Green reaction buffers The universal buffer allows rapid single double or multiple DNA digestion within 5 15 minutes eliminating any need for buffer change or subsequent DNA clean up steps See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity heat inactivation and incubation times for this and other FastDigest restriction enzymes DNA modifying enzymes such as Klenow Fragment T4 DNA Ligase alkaline phosphatases and T4 DNA Polymerase all have 100 activity in FastDigest Buffer Therefore enzymes for downstream applications can be directly added to the FastDigest reaction mix For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects Features• 100 activity of all FastDigest enzymes in the universal buffer• 100 buffer compatibility with downstream applications• Complete digestion in 5 15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism RFLP • SNP analysisNote The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer For applications that require product analysis by fluorescence excitation e g concentration measurements in UV light the colorless FastDigest Buffer is recommended For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/fastdigest mspi/product/Thermo Fisher
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    fastdigest mspi - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Loci under selection and markers associated with host plant and host-related strains shape the genetic structure of Brazilian populations of Spodoptera frugiperda (Lepidoptera, Noctuidae)
    Article Snippet: .. The PCR program included an initial incubation at 94 °C (2 min), followed by 38 cycles of 94 °C (45 s), 56 °C (45 s), 72 °C (1 min), and a final incubation of 72 °C for 5 min. After amplification, 1.0 μL of FastDigest MspI (Thermo Scientific) was added to 10 μL of each reaction, incubated at 37 °C for 10 min, and the complete volume was loaded in a 2% agarose gel in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0). .. Libraries construction and sequencing Genotyping-by-sequencing libraries were constructed using standard protocols [ ], with minor modifications, at the Plateforme d’Analyses Génomiques of the Institut de Biologie Intégrative et des Systèmes (IBIS, Université Laval, Québec city, Canada).

    Article Title: Transcript expression plasticity as a response to alternative larval host plants in the speciation process of corn and rice strains of Spodoptera frugiperda
    Article Snippet: .. After amplification, 1.0 μL of FastDigest MspI (Thermo Scientific, Waltham, MA, USA) was added to 10 μL of each reaction, incubated at 37 °C for 10 min, and the complete volume was loaded in 2% agarose gel in TAE buffer. .. A repeated DNA sequence known as FR [ ] was also amplified for each male and female to confirm their strains, using primers FR-c and FR-2, and conditions described elsewhere [ ], using the same PCR procedure as above.

    Agarose Gel Electrophoresis:

    Article Title: Loci under selection and markers associated with host plant and host-related strains shape the genetic structure of Brazilian populations of Spodoptera frugiperda (Lepidoptera, Noctuidae)
    Article Snippet: .. The PCR program included an initial incubation at 94 °C (2 min), followed by 38 cycles of 94 °C (45 s), 56 °C (45 s), 72 °C (1 min), and a final incubation of 72 °C for 5 min. After amplification, 1.0 μL of FastDigest MspI (Thermo Scientific) was added to 10 μL of each reaction, incubated at 37 °C for 10 min, and the complete volume was loaded in a 2% agarose gel in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0). .. Libraries construction and sequencing Genotyping-by-sequencing libraries were constructed using standard protocols [ ], with minor modifications, at the Plateforme d’Analyses Génomiques of the Institut de Biologie Intégrative et des Systèmes (IBIS, Université Laval, Québec city, Canada).

    Article Title: Transcript expression plasticity as a response to alternative larval host plants in the speciation process of corn and rice strains of Spodoptera frugiperda
    Article Snippet: .. After amplification, 1.0 μL of FastDigest MspI (Thermo Scientific, Waltham, MA, USA) was added to 10 μL of each reaction, incubated at 37 °C for 10 min, and the complete volume was loaded in 2% agarose gel in TAE buffer. .. A repeated DNA sequence known as FR [ ] was also amplified for each male and female to confirm their strains, using primers FR-c and FR-2, and conditions described elsewhere [ ], using the same PCR procedure as above.

    Methylation:

    Article Title: Impact of aerobic exercise and fatty acid supplementation on global and gene-specific DNA methylation
    Article Snippet: .. Briefly, two reactions containing 200 ng of gDNA were set up per sample, one with the methylation-sensitive enzyme FastDigest HpaII and one FastDigest MspI (Thermo Scientific, USA) and incubated for 20 min at 37 °C. .. Following incubation, 13 µL of each reaction were mixed with annealing buffer and added to a separate well of a Pyromark Q24 plate and analyzed using a PyroMark Q24 MDx system (Qiagen, Germany) with the following dispensation order: ACTCGA.

    Purification:

    Article Title: Mapping of γ/δ T cells reveals Vδ2+ T cells resistance to senescence
    Article Snippet: .. In brief, 50 ng of purified DNA was digested with Msp I (Fast digest MspI, Thermo Scientific FD0544, USA) for 30 min at 37 °C followed by heat inactivation at 65 °C for 5 mins. .. Library preparation was performed using NEBNext Ultra DNA library prep kit for Illumina (New England BioLabs, E7370L, USA) and ligated with methylated adapters for Illumina at a dilution of 1:10 (New England BioLabs, E7535L, USA).

    Article Title: Differential incorporation of one-carbon substrates among microbial populations identified by stable isotope probing from the estuary to South China Sea
    Article Snippet: .. Purified PCR products were digested with FastDigest MspI and RsaІ (Thermo Fisher Scientific, Inc.) at 55 °C for 4‒12 h. Digested products were purified by precipitation with ethyl alcohol and then resuspended in 20 μL of sterile deionised water. .. Purified products were then mixed with 0.5 μL of an internal standard and detected on an Applied Biosystems Automated 3730 DNA analyser (Applied Biosystems, USA).

    Article Title: One millimetre makes the difference: high-resolution analysis of methane-oxidizing bacteria and their specific activity at the oxic-anoxic interface in a flooded paddy soil
    Article Snippet: .. The purified PCR product (100 ng) was digested with FastDigest MspI enzyme (Fermentas, St Leon-Rot, Germany) at 37 °C for 6 min. Digested samples were purified with Post-Reaction Clean-Up Spin Columns (Sigma-Aldrich) according to the manufacturer's instructions. .. A total of 2 μl of each purified sample was mixed with 11 μl Hi-Di Formamide (Applied Biosystems, Foster City, CA, USA) and 0.2 μl of an internal DNA fragment length standard (MapMarker 1000, 50–1000 bp, x-rhodamine, Eurogentec, Ougree, Belgium) and denatured for 2 min at 94 °C.

    Incubation:

    Article Title: Loci under selection and markers associated with host plant and host-related strains shape the genetic structure of Brazilian populations of Spodoptera frugiperda (Lepidoptera, Noctuidae)
    Article Snippet: .. The PCR program included an initial incubation at 94 °C (2 min), followed by 38 cycles of 94 °C (45 s), 56 °C (45 s), 72 °C (1 min), and a final incubation of 72 °C for 5 min. After amplification, 1.0 μL of FastDigest MspI (Thermo Scientific) was added to 10 μL of each reaction, incubated at 37 °C for 10 min, and the complete volume was loaded in a 2% agarose gel in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0). .. Libraries construction and sequencing Genotyping-by-sequencing libraries were constructed using standard protocols [ ], with minor modifications, at the Plateforme d’Analyses Génomiques of the Institut de Biologie Intégrative et des Systèmes (IBIS, Université Laval, Québec city, Canada).

    Article Title: Transcript expression plasticity as a response to alternative larval host plants in the speciation process of corn and rice strains of Spodoptera frugiperda
    Article Snippet: .. After amplification, 1.0 μL of FastDigest MspI (Thermo Scientific, Waltham, MA, USA) was added to 10 μL of each reaction, incubated at 37 °C for 10 min, and the complete volume was loaded in 2% agarose gel in TAE buffer. .. A repeated DNA sequence known as FR [ ] was also amplified for each male and female to confirm their strains, using primers FR-c and FR-2, and conditions described elsewhere [ ], using the same PCR procedure as above.

    Article Title: Impact of aerobic exercise and fatty acid supplementation on global and gene-specific DNA methylation
    Article Snippet: .. Briefly, two reactions containing 200 ng of gDNA were set up per sample, one with the methylation-sensitive enzyme FastDigest HpaII and one FastDigest MspI (Thermo Scientific, USA) and incubated for 20 min at 37 °C. .. Following incubation, 13 µL of each reaction were mixed with annealing buffer and added to a separate well of a Pyromark Q24 plate and analyzed using a PyroMark Q24 MDx system (Qiagen, Germany) with the following dispensation order: ACTCGA.

    Polymerase Chain Reaction:

    Article Title: Loci under selection and markers associated with host plant and host-related strains shape the genetic structure of Brazilian populations of Spodoptera frugiperda (Lepidoptera, Noctuidae)
    Article Snippet: .. The PCR program included an initial incubation at 94 °C (2 min), followed by 38 cycles of 94 °C (45 s), 56 °C (45 s), 72 °C (1 min), and a final incubation of 72 °C for 5 min. After amplification, 1.0 μL of FastDigest MspI (Thermo Scientific) was added to 10 μL of each reaction, incubated at 37 °C for 10 min, and the complete volume was loaded in a 2% agarose gel in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0). .. Libraries construction and sequencing Genotyping-by-sequencing libraries were constructed using standard protocols [ ], with minor modifications, at the Plateforme d’Analyses Génomiques of the Institut de Biologie Intégrative et des Systèmes (IBIS, Université Laval, Québec city, Canada).

    Article Title: Differential incorporation of one-carbon substrates among microbial populations identified by stable isotope probing from the estuary to South China Sea
    Article Snippet: .. Purified PCR products were digested with FastDigest MspI and RsaІ (Thermo Fisher Scientific, Inc.) at 55 °C for 4‒12 h. Digested products were purified by precipitation with ethyl alcohol and then resuspended in 20 μL of sterile deionised water. .. Purified products were then mixed with 0.5 μL of an internal standard and detected on an Applied Biosystems Automated 3730 DNA analyser (Applied Biosystems, USA).

    Article Title: One millimetre makes the difference: high-resolution analysis of methane-oxidizing bacteria and their specific activity at the oxic-anoxic interface in a flooded paddy soil
    Article Snippet: .. The purified PCR product (100 ng) was digested with FastDigest MspI enzyme (Fermentas, St Leon-Rot, Germany) at 37 °C for 6 min. Digested samples were purified with Post-Reaction Clean-Up Spin Columns (Sigma-Aldrich) according to the manufacturer's instructions. .. A total of 2 μl of each purified sample was mixed with 11 μl Hi-Di Formamide (Applied Biosystems, Foster City, CA, USA) and 0.2 μl of an internal DNA fragment length standard (MapMarker 1000, 50–1000 bp, x-rhodamine, Eurogentec, Ougree, Belgium) and denatured for 2 min at 94 °C.

    Article Title: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays
    Article Snippet: .. In these tubes, the RFLP mixture consisted of 8.5 μL of doubly distilled water, 0.1-0.5 ng of fresh PCR product, 1 X Fermentas Fast Digest® Buffer (Fermentas, Lithuania), 1 U Fermentas Fast Digest® HinfI restriction enzyme (Fermentas, Lithuania) and 1 U Fermentas Fast Digest® MspI restriction enzyme (Fermentas, Lithuania). ..

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  • 94
    Thermo Fisher mspi
    <t>PCR-RFLP</t> with <t>MspI</t> restriction enzyme
    Mspi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/Thermo Fisher
    Average 94 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    mspi - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    93
    Thermo Fisher fastdigest mspi
    <t>PCR-RFLP</t> with <t>MspI</t> restriction enzyme
    Fastdigest Mspi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest mspi/product/Thermo Fisher
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    fastdigest mspi - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    PCR-RFLP with MspI restriction enzyme

    Journal: Contemporary Oncology

    Article Title: Leptin receptor Q223R polymorphism in Egyptian female patients with breast cancer

    doi: 10.5114/wo.2017.66655

    Figure Lengend Snippet: PCR-RFLP with MspI restriction enzyme

    Article Snippet: The PCR product was digested with MspI (Thermoscientific Fast Digest, cat. No. FD 0014, Lithuania) [ ].

    Techniques: Polymerase Chain Reaction