fastap thermosensitive alkaline phosphatase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fastap thermosensitive alkaline phosphatase
    Fastap Thermosensitive Alkaline Phosphatase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastap thermosensitive alkaline phosphatase/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    fastap thermosensitive alkaline phosphatase - by Bioz Stars, 2020-01
    99/100 stars

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    Centrifugation:

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States). .. The sequencing reactions were purified using the protocol of BigDye Terminator v3.1 Cycle Sequencing kit except centrifugation was performed with 1109 × g and 100 × g and, before adding formamide, samples were dried at +37°C for 10 min. Sequencing was carried out with 3130xl Genetic Analyzer (Applied Biosystems/HITACHI; Foster City, California, United States).

    Amplification:

    Article Title: Two Functional TP53 Genetic Variants and Predisposition to Keloid Scarring in Caucasians
    Article Snippet: .. Subsequently, PCR amplification products were purified using Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher Scientific Inc., Waltham, MA, USA) according to manufacturer procedures. .. Sequencing of the products used BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems, Life Technologies Polska, Warsaw, Poland).

    Article Title: Charcot-Marie-Tooth type 4B2 demyelinating neuropathy in miniature Schnauzer dogs caused by a novel splicing SBF2 (MTMR13) genetic variant: a new spontaneous clinical model
    Article Snippet: Genomic DNA was amplified using a standard touchdown PCR reaction. .. Enzymatic clean-up with Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) was carried out and subsequent sequencing performed.

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: CRISPR locus of pCRISPR-crRNA, pCRISPR-multi-crRNA and pCRISPR-control plasmid were amplified with PCR using one bacterial colony as a template with primers spacerseqF and spacerseqR (Supplementary Table 1). .. PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States).

    Electrophoresis:

    Article Title: Two Functional TP53 Genetic Variants and Predisposition to Keloid Scarring in Caucasians
    Article Snippet: Subsequently, PCR amplification products were purified using Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher Scientific Inc., Waltham, MA, USA) according to manufacturer procedures. .. Electrophoresis and analysis were performed according to manufacturer procedures using an ABI PRISM 3100-Avant machine (Data Collection Software v2.0, Sequencing Analysis Software v5.4; Applied Biosystems).

    Incubation:

    Article Title: The northernmost record of a blood-sucking ectoparasite, Lipoptenafortisetosa Maa ( Diptera: Hippoboscidae), in Estonia
    Article Snippet: The extraction was carried out following the manufacturer’s instructions, with the exception that the first incubation step was 55ºC for two hours rather than one hour. .. PCR products were visualised on a 1.6% agarose gel and 10 μl of the PCR solution was treated with FastAP thermosensitive alkaline phosphatase and exonuclease I (Thermo Scientific).

    Article Title: Local regulation of gene expression by lncRNA promoters, transcription, and splicing
    Article Snippet: After transferring quickly to ice, we added 40 μl of a master mix containing 12 μl 5× FNK Buffer (50 mM Tris-HCl pH 7.5, 5 mM MgCl2 , 0.6 mM CaCl2 , 50 mM KCl, 10 mM DTT, 0.01% Triton X-100), 1 μL Murine RNase Inhibitor (NEB), 3 μL FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), 3 μL T4 Polynucleotide Kinase (NEB), and 1 μL TURBO DNase (Life Technologies). .. We incubated this reaction for 37°C for 30 minutes, then cleaned the reaction with MyOne SILANE magnetic beads and eluted in 6 μl of H2 O.

    Article Title: A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency
    Article Snippet: .. FastAP Thermosensitive Alkaline Phosphatase (one unit/µl) and FastAP 10 × buffer (EF0654, Thermo Scientific) were added to samples and incubated for 1 hr at 37°C. .. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and phosphate affinity SDS-PAGE ( ) were conducted according to standard methods.

    Expressing:

    Article Title: A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency
    Article Snippet: Cultures expressing the different VGLUT1 mutants were collected on DIV 17 with 1 × PBS. .. FastAP Thermosensitive Alkaline Phosphatase (one unit/µl) and FastAP 10 × buffer (EF0654, Thermo Scientific) were added to samples and incubated for 1 hr at 37°C.

    Touchdown PCR:

    Article Title: Charcot-Marie-Tooth type 4B2 demyelinating neuropathy in miniature Schnauzer dogs caused by a novel splicing SBF2 (MTMR13) genetic variant: a new spontaneous clinical model
    Article Snippet: Genomic DNA was amplified using a standard touchdown PCR reaction. .. Enzymatic clean-up with Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) was carried out and subsequent sequencing performed.

    Modification:

    Article Title: Local regulation of gene expression by lncRNA promoters, transcription, and splicing
    Article Snippet: After transferring quickly to ice, we added 40 μl of a master mix containing 12 μl 5× FNK Buffer (50 mM Tris-HCl pH 7.5, 5 mM MgCl2 , 0.6 mM CaCl2 , 50 mM KCl, 10 mM DTT, 0.01% Triton X-100), 1 μL Murine RNase Inhibitor (NEB), 3 μL FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), 3 μL T4 Polynucleotide Kinase (NEB), and 1 μL TURBO DNase (Life Technologies). .. We proceeded with the library preparation as previously described , with one additional modification.

    Western Blot:

    Article Title: A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency
    Article Snippet: FastAP Thermosensitive Alkaline Phosphatase (one unit/µl) and FastAP 10 × buffer (EF0654, Thermo Scientific) were added to samples and incubated for 1 hr at 37°C. .. Western blotting was performed according to standard procedures using HRP-coupled secondary antibodies for qualitative detection.

    Transformation Assay:

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: Escape mutants The survived escape mutant colonies from the conjugation and transformation were re-isolated by plating them on chloramphenicol-ampicillin. .. PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States).

    Conjugation Assay:

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: Escape mutants The survived escape mutant colonies from the conjugation and transformation were re-isolated by plating them on chloramphenicol-ampicillin. .. PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States).

    Ligation:

    Article Title: Local regulation of gene expression by lncRNA promoters, transcription, and splicing
    Article Snippet: After transferring quickly to ice, we added 40 μl of a master mix containing 12 μl 5× FNK Buffer (50 mM Tris-HCl pH 7.5, 5 mM MgCl2 , 0.6 mM CaCl2 , 50 mM KCl, 10 mM DTT, 0.01% Triton X-100), 1 μL Murine RNase Inhibitor (NEB), 3 μL FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), 3 μL T4 Polynucleotide Kinase (NEB), and 1 μL TURBO DNase (Life Technologies). .. To simplify the library preparation for many samples, we added unique sample barcodes (8 nt) during the first adapter ligation .

    Protease Inhibitor:

    Article Title: A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency
    Article Snippet: Both buffers were supplemented with protease inhibitor cocktail (539134, Millipore) and Halt phosphatase Inhibitor Cocktail (78420, Thermo Fisher Scientific). .. FastAP Thermosensitive Alkaline Phosphatase (one unit/µl) and FastAP 10 × buffer (EF0654, Thermo Scientific) were added to samples and incubated for 1 hr at 37°C.

    Transferring:

    Article Title: Local regulation of gene expression by lncRNA promoters, transcription, and splicing
    Article Snippet: .. After transferring quickly to ice, we added 40 μl of a master mix containing 12 μl 5× FNK Buffer (50 mM Tris-HCl pH 7.5, 5 mM MgCl2 , 0.6 mM CaCl2 , 50 mM KCl, 10 mM DTT, 0.01% Triton X-100), 1 μL Murine RNase Inhibitor (NEB), 3 μL FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), 3 μL T4 Polynucleotide Kinase (NEB), and 1 μL TURBO DNase (Life Technologies). .. We incubated this reaction for 37°C for 30 minutes, then cleaned the reaction with MyOne SILANE magnetic beads and eluted in 6 μl of H2 O.

    Generated:

    Article Title: Local regulation of gene expression by lncRNA promoters, transcription, and splicing
    Article Snippet: We generated RNA sequencing libraries as previously described , , with some modifications for high sample throughput. .. After transferring quickly to ice, we added 40 μl of a master mix containing 12 μl 5× FNK Buffer (50 mM Tris-HCl pH 7.5, 5 mM MgCl2 , 0.6 mM CaCl2 , 50 mM KCl, 10 mM DTT, 0.01% Triton X-100), 1 μL Murine RNase Inhibitor (NEB), 3 μL FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), 3 μL T4 Polynucleotide Kinase (NEB), and 1 μL TURBO DNase (Life Technologies).

    Sequencing:

    Article Title: The northernmost record of a blood-sucking ectoparasite, Lipoptenafortisetosa Maa ( Diptera: Hippoboscidae), in Estonia
    Article Snippet: PCR products were visualised on a 1.6% agarose gel and 10 μl of the PCR solution was treated with FastAP thermosensitive alkaline phosphatase and exonuclease I (Thermo Scientific). .. The DNA cycle sequencing was performed in a total volume of 10 μl using BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA).

    Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range
    Article Snippet: A total of 60 samples, which were determined to belong to different individuals as per the microsatellite analysis, were used to sequence a 271 bp stretch of the mitochondrial control region. .. PCRs were performed in a T1 plus Thermocycler (Biometra GmbH, Göttingen, Germany) with an initial denaturation step of 95 °C for 5 min, followed by 38 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 1 min, and a final elongation step of 72 °C for 7 min. PCR products were purified by adding 4 µl of Exonuclease I and 1.6 µl of FastAP™ Thermosensitive Alkaline Phosphatase (Thermo Scientific, Waltham, MA USA) at 37 °C for 15 min followed by 80 °C for 15 min. Purified PCR products were diluted 1:40 and both strands sequenced on an ABI3730 DNA Analyzer (Life Technologies GmbH, Darmstadt, Germany).

    Article Title: Two Functional TP53 Genetic Variants and Predisposition to Keloid Scarring in Caucasians
    Article Snippet: Amplification of the 540-bp TP53 sequence including rs1042522 and rs17878362 was performed by using PCR with 5′-AACCCCAGCCCCCTAGCAGAGACC-3′ as the forward primer and 5′-GGGGATACGG CCAGGCATTGAAGT-3′ as the reverse primer. .. Subsequently, PCR amplification products were purified using Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher Scientific Inc., Waltham, MA, USA) according to manufacturer procedures.

    Article Title: Charcot-Marie-Tooth type 4B2 demyelinating neuropathy in miniature Schnauzer dogs caused by a novel splicing SBF2 (MTMR13) genetic variant: a new spontaneous clinical model
    Article Snippet: .. Enzymatic clean-up with Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) was carried out and subsequent sequencing performed. .. PCR primers were used for sequencing along with BigDye Terminator v.1.1 cycle sequencing kit (Applied Biosystems).

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States). .. Sequencing-PCR of ExoSAP-treated DNA was performed with BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems; Foster City, California, United States) according to the manufacturer’s protocol.

    DNA Extraction:

    Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range
    Article Snippet: Paragraph title: DNA extraction, microsatellite and mitochondrial analysis ... PCRs were performed in a T1 plus Thermocycler (Biometra GmbH, Göttingen, Germany) with an initial denaturation step of 95 °C for 5 min, followed by 38 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 1 min, and a final elongation step of 72 °C for 7 min. PCR products were purified by adding 4 µl of Exonuclease I and 1.6 µl of FastAP™ Thermosensitive Alkaline Phosphatase (Thermo Scientific, Waltham, MA USA) at 37 °C for 15 min followed by 80 °C for 15 min. Purified PCR products were diluted 1:40 and both strands sequenced on an ABI3730 DNA Analyzer (Life Technologies GmbH, Darmstadt, Germany).

    Article Title: Two Functional TP53 Genetic Variants and Predisposition to Keloid Scarring in Caucasians
    Article Snippet: Genomic DNA was extracted either from peripheral blood leukocytes (keloid patients) or from cordial blood leukocytes (newborn infants) using a commercially available DNA isolation kit (QIAamp Blood DNA Mini Kit, QIAGEN, Germany). .. Subsequently, PCR amplification products were purified using Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher Scientific Inc., Waltham, MA, USA) according to manufacturer procedures.

    RNA Sequencing Assay:

    Article Title: Local regulation of gene expression by lncRNA promoters, transcription, and splicing
    Article Snippet: Paragraph title: RNA sequencing libraries. ... After transferring quickly to ice, we added 40 μl of a master mix containing 12 μl 5× FNK Buffer (50 mM Tris-HCl pH 7.5, 5 mM MgCl2 , 0.6 mM CaCl2 , 50 mM KCl, 10 mM DTT, 0.01% Triton X-100), 1 μL Murine RNase Inhibitor (NEB), 3 μL FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), 3 μL T4 Polynucleotide Kinase (NEB), and 1 μL TURBO DNase (Life Technologies).

    Magnetic Beads:

    Article Title: Local regulation of gene expression by lncRNA promoters, transcription, and splicing
    Article Snippet: We enriched for poly(A)+ RNA using oligo d(T)25 magnetic beads (NEB) and eluted in 18 μl H2 O. .. After transferring quickly to ice, we added 40 μl of a master mix containing 12 μl 5× FNK Buffer (50 mM Tris-HCl pH 7.5, 5 mM MgCl2 , 0.6 mM CaCl2 , 50 mM KCl, 10 mM DTT, 0.01% Triton X-100), 1 μL Murine RNase Inhibitor (NEB), 3 μL FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), 3 μL T4 Polynucleotide Kinase (NEB), and 1 μL TURBO DNase (Life Technologies).

    Mutagenesis:

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: Escape mutants The survived escape mutant colonies from the conjugation and transformation were re-isolated by plating them on chloramphenicol-ampicillin. .. PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States).

    Isolation:

    Article Title: Local regulation of gene expression by lncRNA promoters, transcription, and splicing
    Article Snippet: We isolated RNA from harvested mESCs using RNeasy 96 columns. .. After transferring quickly to ice, we added 40 μl of a master mix containing 12 μl 5× FNK Buffer (50 mM Tris-HCl pH 7.5, 5 mM MgCl2 , 0.6 mM CaCl2 , 50 mM KCl, 10 mM DTT, 0.01% Triton X-100), 1 μL Murine RNase Inhibitor (NEB), 3 μL FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), 3 μL T4 Polynucleotide Kinase (NEB), and 1 μL TURBO DNase (Life Technologies).

    Mouse Assay:

    Article Title: A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency
    Article Snippet: Brains of wild type adult mice were dissected for the collection of brain regions. .. FastAP Thermosensitive Alkaline Phosphatase (one unit/µl) and FastAP 10 × buffer (EF0654, Thermo Scientific) were added to samples and incubated for 1 hr at 37°C.

    Polymerase Chain Reaction:

    Article Title: The northernmost record of a blood-sucking ectoparasite, Lipoptenafortisetosa Maa ( Diptera: Hippoboscidae), in Estonia
    Article Snippet: .. PCR products were visualised on a 1.6% agarose gel and 10 μl of the PCR solution was treated with FastAP thermosensitive alkaline phosphatase and exonuclease I (Thermo Scientific). .. One unit of both enzymes was added to the PCR solution, which was incubated for 15 min at 37°C, followed by 15 min inactivation at 80°C.

    Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range
    Article Snippet: .. PCRs were performed in a T1 plus Thermocycler (Biometra GmbH, Göttingen, Germany) with an initial denaturation step of 95 °C for 5 min, followed by 38 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 1 min, and a final elongation step of 72 °C for 7 min. PCR products were purified by adding 4 µl of Exonuclease I and 1.6 µl of FastAP™ Thermosensitive Alkaline Phosphatase (Thermo Scientific, Waltham, MA USA) at 37 °C for 15 min followed by 80 °C for 15 min. Purified PCR products were diluted 1:40 and both strands sequenced on an ABI3730 DNA Analyzer (Life Technologies GmbH, Darmstadt, Germany). .. PCR products were purified by adding 4 µl of Exonuclease I and 1.6 µl of FastAP™ Thermosensitive Alkaline Phosphatase (Thermo Scientific, Waltham, MA USA) at 37 °C for 15 min followed by 80 °C for 15 min.

    Article Title: Two Functional TP53 Genetic Variants and Predisposition to Keloid Scarring in Caucasians
    Article Snippet: .. Subsequently, PCR amplification products were purified using Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher Scientific Inc., Waltham, MA, USA) according to manufacturer procedures. .. Sequencing of the products used BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems, Life Technologies Polska, Warsaw, Poland).

    Article Title: Charcot-Marie-Tooth type 4B2 demyelinating neuropathy in miniature Schnauzer dogs caused by a novel splicing SBF2 (MTMR13) genetic variant: a new spontaneous clinical model
    Article Snippet: Enzymatic clean-up with Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) was carried out and subsequent sequencing performed. .. PCR primers were used for sequencing along with BigDye Terminator v.1.1 cycle sequencing kit (Applied Biosystems).

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: .. PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States). .. Sequencing-PCR of ExoSAP-treated DNA was performed with BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems; Foster City, California, United States) according to the manufacturer’s protocol.

    Polyacrylamide Gel Electrophoresis:

    Article Title: A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency
    Article Snippet: FastAP Thermosensitive Alkaline Phosphatase (one unit/µl) and FastAP 10 × buffer (EF0654, Thermo Scientific) were added to samples and incubated for 1 hr at 37°C. .. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and phosphate affinity SDS-PAGE ( ) were conducted according to standard methods.

    CRISPR:

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: CRISPR locus of pCRISPR-crRNA, pCRISPR-multi-crRNA and pCRISPR-control plasmid were amplified with PCR using one bacterial colony as a template with primers spacerseqF and spacerseqR (Supplementary Table 1). .. PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States).

    Purification:

    Article Title: The northernmost record of a blood-sucking ectoparasite, Lipoptenafortisetosa Maa ( Diptera: Hippoboscidae), in Estonia
    Article Snippet: PCR was performed in a total volume of 20 μl, with the reaction mixture containing 1X BD Advantage 2 PCR buffer, 1U BD Advantage 2 Polymerase mix (BD Biosciences, San Jose, USA), 0.2 mM dNTP (Thermo Scientific, Pittsburgh, USA), 5 pmol of primers LCO1490 (5'-ggtcaacaaatcataaagatattgg-3') and HC02198 (5'-taaacttcagggtgaccaaaaaatca-3') ( ) (replaced by MLepF1 (5’- GCTTTCCCACGAATAAATAATA-3’) ( ) and LepR1 (5’-TAAACTTCTGGATGTCCAAAAAATCA-3’) ( ) for degraded samples) and 20–80 ng of purified genomic DNA. .. PCR products were visualised on a 1.6% agarose gel and 10 μl of the PCR solution was treated with FastAP thermosensitive alkaline phosphatase and exonuclease I (Thermo Scientific).

    Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range
    Article Snippet: .. PCRs were performed in a T1 plus Thermocycler (Biometra GmbH, Göttingen, Germany) with an initial denaturation step of 95 °C for 5 min, followed by 38 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 1 min, and a final elongation step of 72 °C for 7 min. PCR products were purified by adding 4 µl of Exonuclease I and 1.6 µl of FastAP™ Thermosensitive Alkaline Phosphatase (Thermo Scientific, Waltham, MA USA) at 37 °C for 15 min followed by 80 °C for 15 min. Purified PCR products were diluted 1:40 and both strands sequenced on an ABI3730 DNA Analyzer (Life Technologies GmbH, Darmstadt, Germany). .. PCR products were purified by adding 4 µl of Exonuclease I and 1.6 µl of FastAP™ Thermosensitive Alkaline Phosphatase (Thermo Scientific, Waltham, MA USA) at 37 °C for 15 min followed by 80 °C for 15 min.

    Article Title: Two Functional TP53 Genetic Variants and Predisposition to Keloid Scarring in Caucasians
    Article Snippet: .. Subsequently, PCR amplification products were purified using Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher Scientific Inc., Waltham, MA, USA) according to manufacturer procedures. .. Sequencing of the products used BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems, Life Technologies Polska, Warsaw, Poland).

    Article Title: Charcot-Marie-Tooth type 4B2 demyelinating neuropathy in miniature Schnauzer dogs caused by a novel splicing SBF2 (MTMR13) genetic variant: a new spontaneous clinical model
    Article Snippet: Enzymatic clean-up with Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) was carried out and subsequent sequencing performed. .. Sequencing reaction products were purified using sephadex columns and then run on a 3730xl DNA Analyzer.

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: .. PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States). .. Sequencing-PCR of ExoSAP-treated DNA was performed with BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems; Foster City, California, United States) according to the manufacturer’s protocol.

    SDS Page:

    Article Title: A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency
    Article Snippet: FastAP Thermosensitive Alkaline Phosphatase (one unit/µl) and FastAP 10 × buffer (EF0654, Thermo Scientific) were added to samples and incubated for 1 hr at 37°C. .. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and phosphate affinity SDS-PAGE ( ) were conducted according to standard methods.

    Plasmid Preparation:

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: CRISPR locus of pCRISPR-crRNA, pCRISPR-multi-crRNA and pCRISPR-control plasmid were amplified with PCR using one bacterial colony as a template with primers spacerseqF and spacerseqR (Supplementary Table 1). .. PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States).

    Software:

    Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range
    Article Snippet: PCR products were run in an automated sequencer (ABI 310) and genotypes were determined using ABI Genescan and Genotyper version 2.1 software. .. PCRs were performed in a T1 plus Thermocycler (Biometra GmbH, Göttingen, Germany) with an initial denaturation step of 95 °C for 5 min, followed by 38 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 1 min, and a final elongation step of 72 °C for 7 min. PCR products were purified by adding 4 µl of Exonuclease I and 1.6 µl of FastAP™ Thermosensitive Alkaline Phosphatase (Thermo Scientific, Waltham, MA USA) at 37 °C for 15 min followed by 80 °C for 15 min. Purified PCR products were diluted 1:40 and both strands sequenced on an ABI3730 DNA Analyzer (Life Technologies GmbH, Darmstadt, Germany).

    Article Title: Two Functional TP53 Genetic Variants and Predisposition to Keloid Scarring in Caucasians
    Article Snippet: Subsequently, PCR amplification products were purified using Exonuclease I and FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher Scientific Inc., Waltham, MA, USA) according to manufacturer procedures. .. Electrophoresis and analysis were performed according to manufacturer procedures using an ABI PRISM 3100-Avant machine (Data Collection Software v2.0, Sequencing Analysis Software v5.4; Applied Biosystems).

    Article Title: Midbiotics: conjugative plasmids for genetic engineering of natural gut flora
    Article Snippet: PCR product was purified from primers and nucleotides with 0.4 U of Exonuclease I (20 U/µl, ThermoScientific; Waltham, Massachusetts, United States) and 0.4 U of FastAP Thermosensitive Alkaline phosphatase (1U/µl, ThermoScientific; Waltham, Massachusetts, United States). .. The basecalling was performed with Sequencing Analysis Software v6.0 (Applied Biosystems; Foster City, California, United States), and the sequences were analyzed for deletions or mutations in CRISPR locus by mapping them against the original sequence by using Geneious 8.1.9 (Biomatters Ltd; Auckland, New Zealand).

    Agarose Gel Electrophoresis:

    Article Title: The northernmost record of a blood-sucking ectoparasite, Lipoptenafortisetosa Maa ( Diptera: Hippoboscidae), in Estonia
    Article Snippet: .. PCR products were visualised on a 1.6% agarose gel and 10 μl of the PCR solution was treated with FastAP thermosensitive alkaline phosphatase and exonuclease I (Thermo Scientific). .. One unit of both enzymes was added to the PCR solution, which was incubated for 15 min at 37°C, followed by 15 min inactivation at 80°C.

    Homogenization:

    Article Title: A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency
    Article Snippet: The samples were treated with homogenization buffer (0.32 M sucrose, 4 mM HEPES pH 7.4). .. FastAP Thermosensitive Alkaline Phosphatase (one unit/µl) and FastAP 10 × buffer (EF0654, Thermo Scientific) were added to samples and incubated for 1 hr at 37°C.

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  • 90
    Thermo Fisher fastap thermosensitive alkaline phosphatase
    Fastap Thermosensitive Alkaline Phosphatase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastap thermosensitive alkaline phosphatase/product/Thermo Fisher
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    fastap thermosensitive alkaline phosphatase - by Bioz Stars, 2020-01
    90/100 stars
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