fast universal pcr master mix  (Thermo Fisher)


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    Name:
    PCR Master Mix 2X
    Description:
    Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase dNTPs and all of the components required for PCR except DNA template and primers This pre mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up The mix is optimized for efficient and reproducible PCR Highlights• Convenient ready to use mix• Thermostable half life is more than 40 min at 95°C• Generates PCR products with 3 dA overhangs• Incorporates modified nucleotides e g biotin digoxigenin fluorescently labeled nucleotides Applications• Routine PCR amplification of DNA fragments up to 5 kb• High throughput PCR• DNA labelingNote• The error rate of Taq DNA Polymerase in PCR is 2 2 x 10 5 errors per nt per cycle as determined by a modified method described in Accordingly the accuracy of PCR is 4 5 x 104 Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs
    Catalog Number:
    k0171
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|Routine PCR
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher fast universal pcr master mix
    WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, <t>cDNA</t> synthesized, and gene expression analyzed by <t>qRT-PCR.</t> Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p
    Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase dNTPs and all of the components required for PCR except DNA template and primers This pre mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up The mix is optimized for efficient and reproducible PCR Highlights• Convenient ready to use mix• Thermostable half life is more than 40 min at 95°C• Generates PCR products with 3 dA overhangs• Incorporates modified nucleotides e g biotin digoxigenin fluorescently labeled nucleotides Applications• Routine PCR amplification of DNA fragments up to 5 kb• High throughput PCR• DNA labelingNote• The error rate of Taq DNA Polymerase in PCR is 2 2 x 10 5 errors per nt per cycle as determined by a modified method described in Accordingly the accuracy of PCR is 4 5 x 104 Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs
    https://www.bioz.com/result/fast universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    fast universal pcr master mix - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms"

    Article Title: WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms

    Journal: Journal of Neuroinflammation

    doi: 10.1186/s12974-017-1045-9

    WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p
    Figure Legend Snippet: WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p

    Techniques Used: Isolation, Synthesized, Expressing, Quantitative RT-PCR

    2) Product Images from "WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms"

    Article Title: WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms

    Journal: Journal of Neuroinflammation

    doi: 10.1186/s12974-017-1045-9

    WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p
    Figure Legend Snippet: WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p

    Techniques Used: Isolation, Synthesized, Expressing, Quantitative RT-PCR

    3) Product Images from "Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer"

    Article Title: Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.032250

    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).
    Figure Legend Snippet: Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Techniques Used: Histone Deacetylase Assay, Quantitative RT-PCR, Expressing

    4) Product Images from "Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer"

    Article Title: Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.032250

    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).
    Figure Legend Snippet: Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Techniques Used: Histone Deacetylase Assay, Quantitative RT-PCR, Expressing

    Related Articles

    Amplification:

    Article Title: The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3?-end processing of animal histone mRNAs
    Article Snippet: .. For real-time PCR, reverse-transcribed material corresponding to 40 ng RNA was amplified in 25 μl Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) with specific primers, and TaqMan probes by using the ABI SDS7000 Sequence Detection System. ..

    Article Title: Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia
    Article Snippet: .. For realtime PCR, reverse transcribed material corresponding to 40 ng RNA was amplified with the TaqMan assays described below in 25 μl Universal PCR Master Mix, No AmpErase UNG on the SDS 7000 (Applied Biosystems) using the standard thermal protocol. ..

    Polymerase Chain Reaction:

    Article Title: Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs
    Article Snippet: .. Real-time qPCR was used to quantify mRNA expression and chromosomal promoter DNA content (following immunoprecipiation; see ChIP) using Taqman gene expression assays ( ; Applied Biosystems, Grand Island, NY) and Taqman 2× PCR Master mix (Applied Biosystems). β-Actin mRNA levels were used as an endogenous control for normalization (mouse or human ACTB Endogenous Control FAM-MGB Probe, 4352933 and 4352935, respectively; Applied Biosystems). .. Real-time qPCR was carried out in an ABI STEP One Plus Prism 7700, and the data were analyzed with the corresponding software (Applied Biosystems).

    Article Title: The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3?-end processing of animal histone mRNAs
    Article Snippet: .. For real-time PCR, reverse-transcribed material corresponding to 40 ng RNA was amplified in 25 μl Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) with specific primers, and TaqMan probes by using the ABI SDS7000 Sequence Detection System. ..

    Article Title: The iFat1 transgene permits conditional endogenous n-3 PUFA enrichment both in vitro and in vivo
    Article Snippet: .. To screen samples for presence of the Tam-Cre transgene, PCR master mix was prepared to a final concentration of 0.2 mM dNTP (ThermoScientific Fermentas R0192), 2 mM MgCl2 and 0.625 units Platinum® Taq DNA polymerase (Invitrogen 10966-034) and 0.5 μM each of specified primers (Ventura et al. ). .. Samples were incubated at 94 °C for 180 s, followed by 35 cycles of 94 °C for 30 s, 58 °C for 90 s and 72 °C for 60 s. Samples were held at a final extension temperature of 72 °C for 300 s. For iFat1 reactions, PCR master mix was composed of 0.3 mM dNTP, 10× PCR buffer, 1.5 mM MgCl2 and 1.5 units of Platinum ® Taq and 0.4 μM each of: 5′-ACGTCAGTAGTCATAGGAACTGCGGTCG-3′(F) and 5′-CCAACCGGTGGGACATTT GAGTTG-3′(R).

    Article Title: Regulator of G protein signaling 2 is a functionally important negative regulator of angiotensin II-induced cardiac fibroblast responses
    Article Snippet: .. Reverse-transcribed (TaqMan reverse transcription reagents) RNA samples from fibroblasts were subjected to real-time PCR using FAM-labeled TaqMan probes for RGS2, RGS3, RGS5, GAPDH, and 18S and universal PCR master mix according to the manufacturer's instructions (Applied Biosystems, Carlsbad, CA). .. Each sample was assayed in duplicate in two independent PCR reactions and normalized to 18S expression.

    Article Title: Characterization of the transcripts and protein isoforms for cytoplasmic polyadenylation element binding protein-3 (CPEB3) in the mouse retina
    Article Snippet: .. PCR master mix containing Taq DNA polymerase, dNTPs and optimized reaction buffer were also obtained from Applied Biosystems. .. PCR reactions were performed with an ABI 7300 real-time PCR system using the following conditions: 50°C, 2 min; 95°C, 10 min; 40 cycles of (95°C, 15 sec; 60°C, 1 min).

    Article Title: Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia
    Article Snippet: .. For realtime PCR, reverse transcribed material corresponding to 40 ng RNA was amplified with the TaqMan assays described below in 25 μl Universal PCR Master Mix, No AmpErase UNG on the SDS 7000 (Applied Biosystems) using the standard thermal protocol. ..

    Article Title: ING1b-inducible microRNA203 inhibits cell proliferation
    Article Snippet: .. The TaqMan Array Human MicroRNA Card Set v3.0, TaqMan MicroRNA Reverse Transcription Kit, the primers for miRNAs and U6B and universal PCR Master Mix were purchased from Applied Biosystems (Foster City, CA, USA). .. MicroRNA mimics and MicroRNA mimic controls were purchased from Dharmacon (Lafayette, CO, USA).

    Sequencing:

    Article Title: The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3?-end processing of animal histone mRNAs
    Article Snippet: .. For real-time PCR, reverse-transcribed material corresponding to 40 ng RNA was amplified in 25 μl Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) with specific primers, and TaqMan probes by using the ABI SDS7000 Sequence Detection System. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs
    Article Snippet: .. Real-time qPCR was used to quantify mRNA expression and chromosomal promoter DNA content (following immunoprecipiation; see ChIP) using Taqman gene expression assays ( ; Applied Biosystems, Grand Island, NY) and Taqman 2× PCR Master mix (Applied Biosystems). β-Actin mRNA levels were used as an endogenous control for normalization (mouse or human ACTB Endogenous Control FAM-MGB Probe, 4352933 and 4352935, respectively; Applied Biosystems). .. Real-time qPCR was carried out in an ABI STEP One Plus Prism 7700, and the data were analyzed with the corresponding software (Applied Biosystems).

    Article Title: The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3?-end processing of animal histone mRNAs
    Article Snippet: .. For real-time PCR, reverse-transcribed material corresponding to 40 ng RNA was amplified in 25 μl Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) with specific primers, and TaqMan probes by using the ABI SDS7000 Sequence Detection System. ..

    Article Title: Regulator of G protein signaling 2 is a functionally important negative regulator of angiotensin II-induced cardiac fibroblast responses
    Article Snippet: .. Reverse-transcribed (TaqMan reverse transcription reagents) RNA samples from fibroblasts were subjected to real-time PCR using FAM-labeled TaqMan probes for RGS2, RGS3, RGS5, GAPDH, and 18S and universal PCR master mix according to the manufacturer's instructions (Applied Biosystems, Carlsbad, CA). .. Each sample was assayed in duplicate in two independent PCR reactions and normalized to 18S expression.

    Concentration Assay:

    Article Title: The iFat1 transgene permits conditional endogenous n-3 PUFA enrichment both in vitro and in vivo
    Article Snippet: .. To screen samples for presence of the Tam-Cre transgene, PCR master mix was prepared to a final concentration of 0.2 mM dNTP (ThermoScientific Fermentas R0192), 2 mM MgCl2 and 0.625 units Platinum® Taq DNA polymerase (Invitrogen 10966-034) and 0.5 μM each of specified primers (Ventura et al. ). .. Samples were incubated at 94 °C for 180 s, followed by 35 cycles of 94 °C for 30 s, 58 °C for 90 s and 72 °C for 60 s. Samples were held at a final extension temperature of 72 °C for 300 s. For iFat1 reactions, PCR master mix was composed of 0.3 mM dNTP, 10× PCR buffer, 1.5 mM MgCl2 and 1.5 units of Platinum ® Taq and 0.4 μM each of: 5′-ACGTCAGTAGTCATAGGAACTGCGGTCG-3′(F) and 5′-CCAACCGGTGGGACATTT GAGTTG-3′(R).

    Expressing:

    Article Title: Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs
    Article Snippet: .. Real-time qPCR was used to quantify mRNA expression and chromosomal promoter DNA content (following immunoprecipiation; see ChIP) using Taqman gene expression assays ( ; Applied Biosystems, Grand Island, NY) and Taqman 2× PCR Master mix (Applied Biosystems). β-Actin mRNA levels were used as an endogenous control for normalization (mouse or human ACTB Endogenous Control FAM-MGB Probe, 4352933 and 4352935, respectively; Applied Biosystems). .. Real-time qPCR was carried out in an ABI STEP One Plus Prism 7700, and the data were analyzed with the corresponding software (Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Tumor Suppressor Role for the SPOP Ubiquitin Ligase in Signal-Dependent Proteolysis of the Oncogenic Coactivator SRC-3/AIB1
    Article Snippet: .. Each TaqMan probe for its corresponding target gene mRNA and One-Step RT-PCR master mix also were from Applied Biosystems. .. Soft Agar Assay This assay was performed according to Cell Transformation Detection Assay (Chemicon International).

    Chromatin Immunoprecipitation:

    Article Title: Differential Regulation of Vascular Endothelial Growth Factors by Promoter-targeted shRNAs
    Article Snippet: .. Real-time qPCR was used to quantify mRNA expression and chromosomal promoter DNA content (following immunoprecipiation; see ChIP) using Taqman gene expression assays ( ; Applied Biosystems, Grand Island, NY) and Taqman 2× PCR Master mix (Applied Biosystems). β-Actin mRNA levels were used as an endogenous control for normalization (mouse or human ACTB Endogenous Control FAM-MGB Probe, 4352933 and 4352935, respectively; Applied Biosystems). .. Real-time qPCR was carried out in an ABI STEP One Plus Prism 7700, and the data were analyzed with the corresponding software (Applied Biosystems).

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  • 99
    Thermo Fisher fast universal pcr master mix
    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by <t>TaqMan</t> <t>qRT-PCR.</t> Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).
    Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    fast universal pcr master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher taqman fast universal pcr master mix 2×
    Probe and primer design for microfluidic quantitative <t>PCR</t> (MFQPCR) detection. Two assays (SET1 and SET2) were designed for each transgene. Forward and reverse primers for MFQPCR were designed to target different exons. <t>TaqMan</t> probe for MFQPCR was designed to target exon/exon junctions. Forward and reverse primers for pre-amplification were designed to include the forward and reverse primers for MFQPCR. While forward and reverse primers for pre-amplification and MFQPCR may amplify PCR products, including genomic DNA regions, TaqMan probes do not anneal to the PCR product, including genomic DNA regions ( A ). TaqMan probes specifically anneal to PCR products having exon/exon junction sequences ( B ).
    Taqman Fast Universal Pcr Master Mix 2×, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman fast universal pcr master mix 2×/product/Thermo Fisher
    Average 91 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    taqman fast universal pcr master mix 2× - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    99
    Thermo Fisher taqman fast universal pcr master mix
    30% CR affected the MicroRNA expression in mouse liver The expression of indicated miRNAs was assayed using ( A ) microarray hybridization and ( B ) <t>Taqman</t> <t>PCR</t> assay in the liver of AL (Open bars) and CR mice (Black bars). * - statistically significant difference (p
    Taqman Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman fast universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 330 article reviews
    Price from $9.99 to $1999.99
    taqman fast universal pcr master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Journal: Disease Models & Mechanisms

    Article Title: Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer

    doi: 10.1242/dmm.032250

    Figure Lengend Snippet: Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Article Snippet: Two-step qPCR was completed (50-250 ng cDNA template/reaction) using either TaqMan gene expression assays (CTSC, ENG, TIE1, BMP2, BMP4, RPL37A) with Fast Universal PCR Master Mix, no AmpErase™ UNG (Thermo Fisher Scientific) or Fast SYBR™ Green Master Mix (50-250 ng cDNA template/reaction) and the following oligonucleotide primers (Integrated DNA Technologies, Singapore).

    Techniques: Histone Deacetylase Assay, Quantitative RT-PCR, Expressing

    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Journal: Disease Models & Mechanisms

    Article Title: Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer

    doi: 10.1242/dmm.032250

    Figure Lengend Snippet: Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Article Snippet: Two-step qPCR was completed (50-250 ng cDNA template/reaction) using either TaqMan gene expression assays (CTSC, ENG, TIE1, BMP2, BMP4, RPL37A) with Fast Universal PCR Master Mix, no AmpErase™ UNG (Thermo Fisher Scientific) or Fast SYBR™ Green Master Mix (50-250 ng cDNA template/reaction) and the following oligonucleotide primers (Integrated DNA Technologies, Singapore).

    Techniques: Histone Deacetylase Assay, Quantitative RT-PCR, Expressing

    Probe and primer design for microfluidic quantitative PCR (MFQPCR) detection. Two assays (SET1 and SET2) were designed for each transgene. Forward and reverse primers for MFQPCR were designed to target different exons. TaqMan probe for MFQPCR was designed to target exon/exon junctions. Forward and reverse primers for pre-amplification were designed to include the forward and reverse primers for MFQPCR. While forward and reverse primers for pre-amplification and MFQPCR may amplify PCR products, including genomic DNA regions, TaqMan probes do not anneal to the PCR product, including genomic DNA regions ( A ). TaqMan probes specifically anneal to PCR products having exon/exon junction sequences ( B ).

    Journal: Genes

    Article Title: Microfluidic Quantitative PCR Detection of 12 Transgenes from Horse Plasma for Gene Doping Control

    doi: 10.3390/genes11040457

    Figure Lengend Snippet: Probe and primer design for microfluidic quantitative PCR (MFQPCR) detection. Two assays (SET1 and SET2) were designed for each transgene. Forward and reverse primers for MFQPCR were designed to target different exons. TaqMan probe for MFQPCR was designed to target exon/exon junctions. Forward and reverse primers for pre-amplification were designed to include the forward and reverse primers for MFQPCR. While forward and reverse primers for pre-amplification and MFQPCR may amplify PCR products, including genomic DNA regions, TaqMan probes do not anneal to the PCR product, including genomic DNA regions ( A ). TaqMan probes specifically anneal to PCR products having exon/exon junction sequences ( B ).

    Article Snippet: For the sample mix, 1.8 μL of diluted pre-amplified PCR products were combined with 2.0 μL of TaqMan Fast Universal PCR Master Mix (2×), No AmpErase UNG (Thermo Fisher Scientific) and 0.2 μL of 20× GE Sample Loading Reagent (Fluidigm).

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction

    30% CR affected the MicroRNA expression in mouse liver The expression of indicated miRNAs was assayed using ( A ) microarray hybridization and ( B ) Taqman PCR assay in the liver of AL (Open bars) and CR mice (Black bars). * - statistically significant difference (p

    Journal: Aging (Albany NY)

    Article Title: Aging and calorie restriction regulate the expression of miR-125a-5p and its target genes Stat3, Casp2 and Stard13

    doi: 10.18632/aging.101270

    Figure Lengend Snippet: 30% CR affected the MicroRNA expression in mouse liver The expression of indicated miRNAs was assayed using ( A ) microarray hybridization and ( B ) Taqman PCR assay in the liver of AL (Open bars) and CR mice (Black bars). * - statistically significant difference (p

    Article Snippet: Determination of miRNA expression of miR-125a-5p and miR-145b was done by using TaqMan micro RNA assay (ThermoFisher Scientific Catalog # 4427975), and TaqMan Fast Universal PCR master mix 2x (ThermoFisher Scientific Catalog # 4352042) as per the manufacturer's instructions.

    Techniques: Expressing, Microarray, Hybridization, Polymerase Chain Reaction, Mouse Assay