fast universal pcr master mix  (Thermo Fisher)


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    Name:
    PCR Master Mix 2X
    Description:
    Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase dNTPs and all of the components required for PCR except DNA template and primers This pre mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up The mix is optimized for efficient and reproducible PCR Highlights• Convenient ready to use mix• Thermostable half life is more than 40 min at 95°C• Generates PCR products with 3 dA overhangs• Incorporates modified nucleotides e g biotin digoxigenin fluorescently labeled nucleotides Applications• Routine PCR amplification of DNA fragments up to 5 kb• High throughput PCR• DNA labelingNote• The error rate of Taq DNA Polymerase in PCR is 2 2 x 10 5 errors per nt per cycle as determined by a modified method described in Accordingly the accuracy of PCR is 4 5 x 104 Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs
    Catalog Number:
    k0171
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|Routine PCR
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher fast universal pcr master mix
    WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, <t>cDNA</t> synthesized, and gene expression analyzed by <t>qRT-PCR.</t> Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p
    Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase dNTPs and all of the components required for PCR except DNA template and primers This pre mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up The mix is optimized for efficient and reproducible PCR Highlights• Convenient ready to use mix• Thermostable half life is more than 40 min at 95°C• Generates PCR products with 3 dA overhangs• Incorporates modified nucleotides e g biotin digoxigenin fluorescently labeled nucleotides Applications• Routine PCR amplification of DNA fragments up to 5 kb• High throughput PCR• DNA labelingNote• The error rate of Taq DNA Polymerase in PCR is 2 2 x 10 5 errors per nt per cycle as determined by a modified method described in Accordingly the accuracy of PCR is 4 5 x 104 Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs
    https://www.bioz.com/result/fast universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    fast universal pcr master mix - by Bioz Stars, 2020-05
    99/100 stars

    Images

    1) Product Images from "WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms"

    Article Title: WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms

    Journal: Journal of Neuroinflammation

    doi: 10.1186/s12974-017-1045-9

    WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p
    Figure Legend Snippet: WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p

    Techniques Used: Isolation, Synthesized, Expressing, Quantitative RT-PCR

    2) Product Images from "Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer"

    Article Title: Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.032250

    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).
    Figure Legend Snippet: Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Techniques Used: Histone Deacetylase Assay, Quantitative RT-PCR, Expressing

    3) Product Images from "WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms"

    Article Title: WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms

    Journal: Journal of Neuroinflammation

    doi: 10.1186/s12974-017-1045-9

    WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p
    Figure Legend Snippet: WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 ( a ), IL-1β ( b ), and CCL2 ( c ) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (* p

    Techniques Used: Isolation, Synthesized, Expressing, Quantitative RT-PCR

    4) Product Images from "Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer"

    Article Title: Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.032250

    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).
    Figure Legend Snippet: Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Techniques Used: Histone Deacetylase Assay, Quantitative RT-PCR, Expressing

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    Amplification:

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    Article Snippet: .. Quantitative reverse transcriptase PCR (qRT-PCR) was performed as outlined ( ) with TaqMan One-Step RT-PCR Master Mix reagent and MultiScribe RT for single-tube reverse transcription and amplification using a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: Lipid-like Nanoparticles for Small Interfering RNA Delivery to Endothelial Cells
    Article Snippet: .. The synthesized cDNA was amplified by PCR with human specific primers (SHP-1, KDR/Flk-1, eNOS, and β-actin) using Platinum PCR master mix (Invitrogen). .. The amplification conditions followed several steps; 5 min at 95°C, followed by 25–35 cycles of denaturing (94°C, 30 sec), annealing (55–62°C, 30 sec), and extension (72°C, 45 sec) with a final extension at 72°C for 7 min.

    Synthesized:

    Article Title: Lipid-like Nanoparticles for Small Interfering RNA Delivery to Endothelial Cells
    Article Snippet: .. The synthesized cDNA was amplified by PCR with human specific primers (SHP-1, KDR/Flk-1, eNOS, and β-actin) using Platinum PCR master mix (Invitrogen). .. The amplification conditions followed several steps; 5 min at 95°C, followed by 25–35 cycles of denaturing (94°C, 30 sec), annealing (55–62°C, 30 sec), and extension (72°C, 45 sec) with a final extension at 72°C for 7 min.

    Quantitative RT-PCR:

    Article Title: Multiplexed Digital mRNA Profiling of the Inflammatory Response in the West Nile Swiss Webster Mouse Model
    Article Snippet: .. A volume of 20 µl qRT-PCR master mix was prepared per manufacturer instructions (AgPath-ID One-Step RT-PCR Kit, Life Technologies, Foster City, CA). ..

    Article Title: Transcriptional and Phenotypic Responses of Listeria monocytogenes to Chlorine Dioxide
    Article Snippet: .. Quantitative reverse transcriptase PCR (qRT-PCR) was performed as outlined ( ) with TaqMan One-Step RT-PCR Master Mix reagent and MultiScribe RT for single-tube reverse transcription and amplification using a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Multiplexed Digital mRNA Profiling of the Inflammatory Response in the West Nile Swiss Webster Mouse Model
    Article Snippet: .. A volume of 20 µl qRT-PCR master mix was prepared per manufacturer instructions (AgPath-ID One-Step RT-PCR Kit, Life Technologies, Foster City, CA). ..

    Article Title: Transcriptional and Phenotypic Responses of Listeria monocytogenes to Chlorine Dioxide
    Article Snippet: .. Quantitative reverse transcriptase PCR (qRT-PCR) was performed as outlined ( ) with TaqMan One-Step RT-PCR Master Mix reagent and MultiScribe RT for single-tube reverse transcription and amplification using a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: Up-Regulation of Hepatitis C Virus Replication and Production by Inhibition of MEK/ERK Signaling
    Article Snippet: .. Both RNA were quantitated in triplicates using a TaqMan® OneStep RT-PCR Master Mix Reagents Kit (Applied Biosystems, Foster City, CA) and the primers and probes described previously . .. The reactions were performed on a Mx3000P QPCR system (Stratagene, La Jolla, CA) with a program of 48°C for 30 min, 95°C for 10 min, and then 50 cycles at 95°C for 15 sec and 60°C for 1 min.

    Article Title: Extensive and coordinated transcription of noncoding RNAs within cell cycle promoters
    Article Snippet: .. RT-PCR using 50-250 ng of total RNA was performed using the One-Step RT-PCR Master Mix (Applied Biosystems) using Taqman Gene Expression Assays and normalized to GAPDH. .. Strand specific RT-PCR for PANDA was performed using the One Step RT-PCR Master Mix SYBR Green (Stratagene)).

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptional and Phenotypic Responses of Listeria monocytogenes to Chlorine Dioxide
    Article Snippet: .. Quantitative reverse transcriptase PCR (qRT-PCR) was performed as outlined ( ) with TaqMan One-Step RT-PCR Master Mix reagent and MultiScribe RT for single-tube reverse transcription and amplification using a StepOnePlus real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Concentration Assay:

    Article Title: The iFat1 transgene permits conditional endogenous n-3 PUFA enrichment both in vitro and in vivo
    Article Snippet: .. To screen samples for presence of the Tam-Cre transgene, PCR master mix was prepared to a final concentration of 0.2 mM dNTP (ThermoScientific Fermentas R0192), 2 mM MgCl2 and 0.625 units Platinum® Taq DNA polymerase (Invitrogen 10966-034) and 0.5 μM each of specified primers (Ventura et al. ). .. Samples were incubated at 94 °C for 180 s, followed by 35 cycles of 94 °C for 30 s, 58 °C for 90 s and 72 °C for 60 s. Samples were held at a final extension temperature of 72 °C for 300 s. For iFat1 reactions, PCR master mix was composed of 0.3 mM dNTP, 10× PCR buffer, 1.5 mM MgCl2 and 1.5 units of Platinum ® Taq and 0.4 μM each of: 5′-ACGTCAGTAGTCATAGGAACTGCGGTCG-3′(F) and 5′-CCAACCGGTGGGACATTT GAGTTG-3′(R).

    Expressing:

    Article Title: Extensive and coordinated transcription of noncoding RNAs within cell cycle promoters
    Article Snippet: .. RT-PCR using 50-250 ng of total RNA was performed using the One-Step RT-PCR Master Mix (Applied Biosystems) using Taqman Gene Expression Assays and normalized to GAPDH. .. Strand specific RT-PCR for PANDA was performed using the One Step RT-PCR Master Mix SYBR Green (Stratagene)).

    Polymerase Chain Reaction:

    Article Title: The iFat1 transgene permits conditional endogenous n-3 PUFA enrichment both in vitro and in vivo
    Article Snippet: .. To screen samples for presence of the Tam-Cre transgene, PCR master mix was prepared to a final concentration of 0.2 mM dNTP (ThermoScientific Fermentas R0192), 2 mM MgCl2 and 0.625 units Platinum® Taq DNA polymerase (Invitrogen 10966-034) and 0.5 μM each of specified primers (Ventura et al. ). .. Samples were incubated at 94 °C for 180 s, followed by 35 cycles of 94 °C for 30 s, 58 °C for 90 s and 72 °C for 60 s. Samples were held at a final extension temperature of 72 °C for 300 s. For iFat1 reactions, PCR master mix was composed of 0.3 mM dNTP, 10× PCR buffer, 1.5 mM MgCl2 and 1.5 units of Platinum ® Taq and 0.4 μM each of: 5′-ACGTCAGTAGTCATAGGAACTGCGGTCG-3′(F) and 5′-CCAACCGGTGGGACATTT GAGTTG-3′(R).

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    Thermo Fisher fast universal pcr master mix
    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by <t>TaqMan</t> <t>qRT-PCR.</t> Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).
    Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    fast universal pcr master mix - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher taqman fast universal pcr master mix
    30% CR affected the MicroRNA expression in mouse liver The expression of indicated miRNAs was assayed using ( A ) microarray hybridization and ( B ) <t>Taqman</t> <t>PCR</t> assay in the liver of AL (Open bars) and CR mice (Black bars). * - statistically significant difference (p
    Taqman Fast Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman fast universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 930 article reviews
    Price from $9.99 to $1999.99
    taqman fast universal pcr master mix - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Journal: Disease Models & Mechanisms

    Article Title: Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer

    doi: 10.1242/dmm.032250

    Figure Lengend Snippet: Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Article Snippet: Two-step qPCR was completed (50-250 ng cDNA template/reaction) using either TaqMan gene expression assays (CTSC, ENG, TIE1, BMP2, BMP4, RPL37A) with Fast Universal PCR Master Mix, no AmpErase™ UNG (Thermo Fisher Scientific) or Fast SYBR™ Green Master Mix (50-250 ng cDNA template/reaction) and the following oligonucleotide primers (Integrated DNA Technologies, Singapore).

    Techniques: Histone Deacetylase Assay, Quantitative RT-PCR, Expressing

    Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Journal: Disease Models & Mechanisms

    Article Title: Functional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer

    doi: 10.1242/dmm.032250

    Figure Lengend Snippet: Analysis of BMP4 and CST6 mRNA levels following treatment with demethylating agent and histone deacetylase inhibitor. (A) A CpG island (506 bp) spans the transcription start site of the human BMP4 gene. (B) Relative BMP4 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (C) TaqMan qRT-PCR analysis of BMP4 mRNA expression levels in 5-Aza-2′-deoxycytidine (5azadC)- and Trichostatin A (TSA)-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3). (D) A CpG island (370 bp) spans the transcription start site of the human CST6 gene. (E) Relative CST6 mRNA levels in vehicle (DMSO)-treated 231_ATCC and 231_HM.LNm5 cells by TaqMan qRT-PCR. Expression in 231_ATCC was set to 1. Mean±s.d. ( n =3). (F) TaqMan qRT-PCR analysis of CST6 mRNA expression levels in 5azadC- and TSA-treated 231_ATCC and 231_HM.LNm5 cells. Expression in vehicle-treated cells was set to 1. Mean±s.d. ( n =3).

    Article Snippet: Two-step qPCR was completed (50-250 ng cDNA template/reaction) using either TaqMan gene expression assays (CTSC, ENG, TIE1, BMP2, BMP4, RPL37A) with Fast Universal PCR Master Mix, no AmpErase™ UNG (Thermo Fisher Scientific) or Fast SYBR™ Green Master Mix (50-250 ng cDNA template/reaction) and the following oligonucleotide primers (Integrated DNA Technologies, Singapore).

    Techniques: Histone Deacetylase Assay, Quantitative RT-PCR, Expressing

    30% CR affected the MicroRNA expression in mouse liver The expression of indicated miRNAs was assayed using ( A ) microarray hybridization and ( B ) Taqman PCR assay in the liver of AL (Open bars) and CR mice (Black bars). * - statistically significant difference (p

    Journal: Aging (Albany NY)

    Article Title: Aging and calorie restriction regulate the expression of miR-125a-5p and its target genes Stat3, Casp2 and Stard13

    doi: 10.18632/aging.101270

    Figure Lengend Snippet: 30% CR affected the MicroRNA expression in mouse liver The expression of indicated miRNAs was assayed using ( A ) microarray hybridization and ( B ) Taqman PCR assay in the liver of AL (Open bars) and CR mice (Black bars). * - statistically significant difference (p

    Article Snippet: Determination of miRNA expression of miR-125a-5p and miR-145b was done by using TaqMan micro RNA assay (ThermoFisher Scientific Catalog # 4427975), and TaqMan Fast Universal PCR master mix 2x (ThermoFisher Scientific Catalog # 4352042) as per the manufacturer's instructions.

    Techniques: Expressing, Microarray, Hybridization, Polymerase Chain Reaction, Mouse Assay