Structured Review

Santa Cruz Biotechnology fascin
Analysis of Cdc42 and <t>fascin</t> in <t>MYC-nick–expressing</t> cells. ( A ) MYC-nick–overexpressing HFF cells show increased filopodia formation that is further enhanced when Cdc42 is activated. (Magnification: 63×.) ( B ) HCT116 cells overexpressing
Fascin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "MYC-nick promotes cell migration by inducing fascin expression and Cdc42 activation"

Article Title: MYC-nick promotes cell migration by inducing fascin expression and Cdc42 activation

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1610994113

Analysis of Cdc42 and fascin in MYC-nick–expressing cells. ( A ) MYC-nick–overexpressing HFF cells show increased filopodia formation that is further enhanced when Cdc42 is activated. (Magnification: 63×.) ( B ) HCT116 cells overexpressing
Figure Legend Snippet: Analysis of Cdc42 and fascin in MYC-nick–expressing cells. ( A ) MYC-nick–overexpressing HFF cells show increased filopodia formation that is further enhanced when Cdc42 is activated. (Magnification: 63×.) ( B ) HCT116 cells overexpressing

Techniques Used: Expressing

Immunohistochemistry (IHC) of fascin and Cdc42 in human colon cancer biopsies. Representative IHC in normal mucosa, central area of the tumor, and the invasive front of the same tumor is shown. ( A – C ) Cdc42 IHC ( A ), fascin IHC ( B ), and MYC IHC
Figure Legend Snippet: Immunohistochemistry (IHC) of fascin and Cdc42 in human colon cancer biopsies. Representative IHC in normal mucosa, central area of the tumor, and the invasive front of the same tumor is shown. ( A – C ) Cdc42 IHC ( A ), fascin IHC ( B ), and MYC IHC

Techniques Used: Immunohistochemistry

Analysis of fascin expression in MYC-nick induced cell migration. ( A ) Effect of MYC-nick on abundance of endogenous fascin and exogenous GFP-fascin. ( B ) Fascin expression in murine intestinal and colonic lesions derived from different genetic backgrounds
Figure Legend Snippet: Analysis of fascin expression in MYC-nick induced cell migration. ( A ) Effect of MYC-nick on abundance of endogenous fascin and exogenous GFP-fascin. ( B ) Fascin expression in murine intestinal and colonic lesions derived from different genetic backgrounds

Techniques Used: Expressing, Migration, Derivative Assay

2) Product Images from "Grb2-associated binder 2 expression and its roles in uveal melanoma invasion"

Article Title: Grb2-associated binder 2 expression and its roles in uveal melanoma invasion

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2017.7151

Knockdown of Gab2 inhibits the expression of MMP-2, MMP-9, and fascin in UM cells. (A) In the presence of EGF (10 ng/ml), the SCR/MEL202 and siGab/MEL202 cells were cultured for 48 h with serum-free medium on a six-well plate. After culture, the culture
Figure Legend Snippet: Knockdown of Gab2 inhibits the expression of MMP-2, MMP-9, and fascin in UM cells. (A) In the presence of EGF (10 ng/ml), the SCR/MEL202 and siGab/MEL202 cells were cultured for 48 h with serum-free medium on a six-well plate. After culture, the culture

Techniques Used: Expressing, Cell Culture

3) Product Images from "Ubiquitin-proteasome mediated synaptic reorganization - a novel mechanism underlying rapid ischemic tolerance"

Article Title: Ubiquitin-proteasome mediated synaptic reorganization - a novel mechanism underlying rapid ischemic tolerance

Journal:

doi: 10.1523/JNEUROSCI.3474-07.2008

Degradation of MARCKS and Fascin following preconditioning ischemia
Figure Legend Snippet: Degradation of MARCKS and Fascin following preconditioning ischemia

Techniques Used:

4) Product Images from "MicroRNA-30a increases tight junction protein expression to suppress the epithelial-mesenchymal transition and metastasis by targeting Slug in breast cancer"

Article Title: MicroRNA-30a increases tight junction protein expression to suppress the epithelial-mesenchymal transition and metastasis by targeting Slug in breast cancer

Journal: Oncotarget

doi: 10.18632/oncotarget.7656

miR-30a inhibits EMT by binding to Slug ( A ) The cellular phenotype of miR-30a low /Slug high /Fascin high /Claudin low correlates with poor clinicopathological features in breast cancer. Immunostaining results for paraffin-embedded breast cancer tissue samples of LNM-negative and early-stage tumors (stage I and IIa; columns a and b) and LNM-positive and advanced-stage tumors (stages III and IV; columns c and d). In addition, the T/N ratio for miR-30a was
Figure Legend Snippet: miR-30a inhibits EMT by binding to Slug ( A ) The cellular phenotype of miR-30a low /Slug high /Fascin high /Claudin low correlates with poor clinicopathological features in breast cancer. Immunostaining results for paraffin-embedded breast cancer tissue samples of LNM-negative and early-stage tumors (stage I and IIa; columns a and b) and LNM-positive and advanced-stage tumors (stages III and IV; columns c and d). In addition, the T/N ratio for miR-30a was

Techniques Used: Binding Assay, Immunostaining

Claudin expression is enhanced by the miR-30a/Slug axis ( A ) Schematic of the E-boxes in the promoter regions of human CLDN1 , CLDN2 , and CLDN3 . The starting point (+1) indicates the transcription initiation in the open reading frame (ORF) of the gene. ( B ) Western blotting (anti-Slug) of MDA-MB-231 breast cancer cell lysates after lentiviral transduction of miR-30a (left panel). PCR analysis of the genes encoding CLDN-1, -2, and -3 after ChIP in the presence of anti-Slug or anti-IgG from control MDA-MB-231/plemiR cells (con) and MDA-MB-231/plemiR-30a cells (right panel). ( C ) Western blotting revealed that Slug and fascin expression in miR-30a–overexpressing cells following miR-30a knockdown (anti-miR-30a) was inversely correlated with claudin expression. NC, negative control. ( D ) Microfilaments in MDA-MB-231 and Hs578T cells expressing plemiR-30a or the control construct plemiR were detected with Alexa Fluor 488–conjugated phalloidin (green) as indicated by red arrows. ( E ) The number of filopodial tips per cell as averaged from 50 cells per condition was calculated. The data represent the mean ± SD from three independent experiments. *** P
Figure Legend Snippet: Claudin expression is enhanced by the miR-30a/Slug axis ( A ) Schematic of the E-boxes in the promoter regions of human CLDN1 , CLDN2 , and CLDN3 . The starting point (+1) indicates the transcription initiation in the open reading frame (ORF) of the gene. ( B ) Western blotting (anti-Slug) of MDA-MB-231 breast cancer cell lysates after lentiviral transduction of miR-30a (left panel). PCR analysis of the genes encoding CLDN-1, -2, and -3 after ChIP in the presence of anti-Slug or anti-IgG from control MDA-MB-231/plemiR cells (con) and MDA-MB-231/plemiR-30a cells (right panel). ( C ) Western blotting revealed that Slug and fascin expression in miR-30a–overexpressing cells following miR-30a knockdown (anti-miR-30a) was inversely correlated with claudin expression. NC, negative control. ( D ) Microfilaments in MDA-MB-231 and Hs578T cells expressing plemiR-30a or the control construct plemiR were detected with Alexa Fluor 488–conjugated phalloidin (green) as indicated by red arrows. ( E ) The number of filopodial tips per cell as averaged from 50 cells per condition was calculated. The data represent the mean ± SD from three independent experiments. *** P

Techniques Used: Expressing, Western Blot, Multiple Displacement Amplification, Transduction, Polymerase Chain Reaction, Chromatin Immunoprecipitation, Negative Control, Construct

5) Product Images from "Grb2-associated binder 2 expression and its roles in uveal melanoma invasion"

Article Title: Grb2-associated binder 2 expression and its roles in uveal melanoma invasion

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2017.7151

Knockdown of Gab2 inhibits the expression of MMP-2, MMP-9, and fascin in UM cells. (A) In the presence of EGF (10 ng/ml), the SCR/MEL202 and siGab/MEL202 cells were cultured for 48 h with serum-free medium on a six-well plate. After culture, the culture media were concentrated to assess MMP-2 and MMP-9 secretion using ELISA. (B) SCR/MEL202 and siGab2/MEL202 cells were treated with EGF (10 ng/ml) for 5 min. After treatment of cells, proteins were extracted and western blotting using antibody to MMP2 and MMP9 was performed. β-actin was used as a loading control. (C) SCR/MEL202 and siGab2/MEL202 cells were treated with EGF (10 ng/ml) for 5 min. After treatment of cells, proteins were extracted and western blotting using antibody to fascin was performed. Each result is a representative from at least three independent experiments.
Figure Legend Snippet: Knockdown of Gab2 inhibits the expression of MMP-2, MMP-9, and fascin in UM cells. (A) In the presence of EGF (10 ng/ml), the SCR/MEL202 and siGab/MEL202 cells were cultured for 48 h with serum-free medium on a six-well plate. After culture, the culture media were concentrated to assess MMP-2 and MMP-9 secretion using ELISA. (B) SCR/MEL202 and siGab2/MEL202 cells were treated with EGF (10 ng/ml) for 5 min. After treatment of cells, proteins were extracted and western blotting using antibody to MMP2 and MMP9 was performed. β-actin was used as a loading control. (C) SCR/MEL202 and siGab2/MEL202 cells were treated with EGF (10 ng/ml) for 5 min. After treatment of cells, proteins were extracted and western blotting using antibody to fascin was performed. Each result is a representative from at least three independent experiments.

Techniques Used: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot

Related Articles

Western Blot:

Article Title: A Signal Transducer and Activator of Transcription 3·Nuclear Factor κB (Stat3·NFκB) Complex Is Necessary for the Expression of Fascin in Metastatic Breast Cancer Cells in Response to Interleukin (IL)-6 and Tumor Necrosis Factor (TNF)-α *
Article Snippet: .. Antibodies for Western blot analysis were anti-Stat3 (BD Transduction Laboratories), anti-tubulin (Sigma), anti- NFκB p50 and p65, and anti-fascin (Santa Cruz Biotechnology). .. Ligands were used at the following concentrations: 40 ng/ml TNF-α (Millipore) and 20 ng/ml IL-6 combined with 200 ng/ml IL-6 Rα (R & D Systems).

Incubation:

Article Title: Ubiquitin-proteasome mediated synaptic reorganization - a novel mechanism underlying rapid ischemic tolerance
Article Snippet: .. Protein samples (50 μg) were denatured, subject to polyacrylamide gel electrophoresis and transferred to polyvinylodene difluoride membranes (Biorad), prior to incubation with primary antibodies at 4 °C overnight; MARCKS, fascin and actin (Santa Cruz Biotechnology, Santa Cruz CA), MAP2B (Sigma, St Louis MO) NR1, NR2A, NR2B and PSD-95 (BD Biosciences, San Jose, CA) and phospho-CREB (Cell Signaling, Beverly MA). .. Membranes were incubated with anti-rabbit or anti-mouse IgG conjugated to horseradish peroxidase (Cell Signaling Technology, Beverly, MA, USA).

other:

Article Title: GATA3 Transcription Factor Abrogates Smad4 Transcription Factor-mediated Fascin Overexpression, Invadopodium Formation, and Breast Cancer Cell Invasion *
Article Snippet: The following antibodies were used in this study: anti-fascin (#sc-21743), anti-Smad4 (#sc-7154), and anti-GATA3 (#sc-22206) were from Santa Cruz Biotechnology; anti-Smad3 (#9523) was from Cell Signaling; anti-HA (#SAB4300603) and anti-GAPDH (#G8795) were from Sigma.

Polyacrylamide Gel Electrophoresis:

Article Title: Ubiquitin-proteasome mediated synaptic reorganization - a novel mechanism underlying rapid ischemic tolerance
Article Snippet: .. Protein samples (50 μg) were denatured, subject to polyacrylamide gel electrophoresis and transferred to polyvinylodene difluoride membranes (Biorad), prior to incubation with primary antibodies at 4 °C overnight; MARCKS, fascin and actin (Santa Cruz Biotechnology, Santa Cruz CA), MAP2B (Sigma, St Louis MO) NR1, NR2A, NR2B and PSD-95 (BD Biosciences, San Jose, CA) and phospho-CREB (Cell Signaling, Beverly MA). .. Membranes were incubated with anti-rabbit or anti-mouse IgG conjugated to horseradish peroxidase (Cell Signaling Technology, Beverly, MA, USA).

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    Santa Cruz Biotechnology monoclonal rabbit anti fascin
    <t>Fascin</t> knockdown can suppress EMT. The results revealed that Fascin silencing can suppress the EMT of QBC939 cells, which increased <t>E-cadherin</t> expression and inhibited vimentin expression compared to the blank control (BC) and negative control (NC) group. (* P
    Monoclonal Rabbit Anti Fascin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal rabbit anti fascin/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    90
    Santa Cruz Biotechnology fascin
    Analysis of Cdc42 and <t>fascin</t> in <t>MYC-nick–expressing</t> cells. ( A ) MYC-nick–overexpressing HFF cells show increased filopodia formation that is further enhanced when Cdc42 is activated. (Magnification: 63×.) ( B ) HCT116 cells overexpressing
    Fascin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fascin/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fascin - by Bioz Stars, 2020-07
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      Buy from Supplier

    93
    Santa Cruz Biotechnology human anti fascin 1 monoclonal antibody
    The <t>fascin-1</t> protein expression status and progression-free survival of glioma patients.
    Human Anti Fascin 1 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human anti fascin 1 monoclonal antibody/product/Santa Cruz Biotechnology
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
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    89
    Santa Cruz Biotechnology human fascin sirna
    Activation of Fas signaling upregulated <t>Fascin</t> expression in AGS cells through activation of STAT3. The AGS cells were stimulated with 5 μg/ml of anti-Fas in the indicated times. (A) The phosphorylated STAT3 was detected by Western blot. (B) The expression of Fascin mRNA was assayed by real-time PCR. (C) After stimulation with 5 μg/ml anti-Fas for 24 h, the protein level of phosphorylated STAT3 and Fascin in AGS cells was detected by Western blot. (D) The AGS cells were pre-treated with 10 μM of Stattic for 2 h and followed by 5 μg/ml of anti-Fas stimulation for 24 h; the protein level of phosphorylated STAT3 and Fascin was detected by Western blot. After transfection with STAT3 <t>siRNA</t> or NC siRNA for 36 h, (E) the STAT3 expression in the AGS cells was detected by Western blot; (F) the AGS cells were then stimulated with 5 μg/ml of anti-Fas for 2 h, and the Fascin expression in the cells was detected by Western blot. Data are representative of three independent experiments.
    Human Fascin Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    Image Search Results


    Fascin knockdown can suppress EMT. The results revealed that Fascin silencing can suppress the EMT of QBC939 cells, which increased E-cadherin expression and inhibited vimentin expression compared to the blank control (BC) and negative control (NC) group. (* P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Fascin Induces Epithelial-Mesenchymal Transition of Cholangiocarcinoma Cells by Regulating Wnt/β-Catenin Signaling

    doi: 10.12659/MSM.897258

    Figure Lengend Snippet: Fascin knockdown can suppress EMT. The results revealed that Fascin silencing can suppress the EMT of QBC939 cells, which increased E-cadherin expression and inhibited vimentin expression compared to the blank control (BC) and negative control (NC) group. (* P

    Article Snippet: Then, Western blot analysis was carried out using monoclonal (rabbit) anti-Fascin, anti-E-cadherin, anti-vimentin, anti-GSK-3β, anti-phospho-β-catenin (Ser33/37), anti-β-catenin, anti-Histone H1, and anti-β-actin antibody (Santa Cruz Biotechnology, USA).

    Techniques: Expressing, Negative Control

    Fascin-induced EMT is dependent on activation of the Wnt/β-catenin signaling pathway. ( A ). Expression of GSK-3β and phosphorylated β-catenin (Ser33/37) in Fascin knockdown QBC939 cells were detected by Western blot analysis. ( B ). Levels of β-catenin of nuclear fractions in Fascin knockdown QBC939 cells was determined by Western blotting. Histone H1 served as loading controls. ( C ). Expression levels of β-catenin and E-cadherin from plasma membrane fractions of Fascin silencing QBC939 cells were processed for Western blotting analysis. * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Fascin Induces Epithelial-Mesenchymal Transition of Cholangiocarcinoma Cells by Regulating Wnt/β-Catenin Signaling

    doi: 10.12659/MSM.897258

    Figure Lengend Snippet: Fascin-induced EMT is dependent on activation of the Wnt/β-catenin signaling pathway. ( A ). Expression of GSK-3β and phosphorylated β-catenin (Ser33/37) in Fascin knockdown QBC939 cells were detected by Western blot analysis. ( B ). Levels of β-catenin of nuclear fractions in Fascin knockdown QBC939 cells was determined by Western blotting. Histone H1 served as loading controls. ( C ). Expression levels of β-catenin and E-cadherin from plasma membrane fractions of Fascin silencing QBC939 cells were processed for Western blotting analysis. * P

    Article Snippet: Then, Western blot analysis was carried out using monoclonal (rabbit) anti-Fascin, anti-E-cadherin, anti-vimentin, anti-GSK-3β, anti-phospho-β-catenin (Ser33/37), anti-β-catenin, anti-Histone H1, and anti-β-actin antibody (Santa Cruz Biotechnology, USA).

    Techniques: Activation Assay, Expressing, Western Blot

    Analysis of Cdc42 and fascin in MYC-nick–expressing cells. ( A ) MYC-nick–overexpressing HFF cells show increased filopodia formation that is further enhanced when Cdc42 is activated. (Magnification: 63×.) ( B ) HCT116 cells overexpressing

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: MYC-nick promotes cell migration by inducing fascin expression and Cdc42 activation

    doi: 10.1073/pnas.1610994113

    Figure Lengend Snippet: Analysis of Cdc42 and fascin in MYC-nick–expressing cells. ( A ) MYC-nick–overexpressing HFF cells show increased filopodia formation that is further enhanced when Cdc42 is activated. (Magnification: 63×.) ( B ) HCT116 cells overexpressing

    Article Snippet: Antibodies against c-MYC (9E10), Sin3, and fascin were from Santa Cruz Biotechnology.

    Techniques: Expressing

    Immunohistochemistry (IHC) of fascin and Cdc42 in human colon cancer biopsies. Representative IHC in normal mucosa, central area of the tumor, and the invasive front of the same tumor is shown. ( A – C ) Cdc42 IHC ( A ), fascin IHC ( B ), and MYC IHC

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: MYC-nick promotes cell migration by inducing fascin expression and Cdc42 activation

    doi: 10.1073/pnas.1610994113

    Figure Lengend Snippet: Immunohistochemistry (IHC) of fascin and Cdc42 in human colon cancer biopsies. Representative IHC in normal mucosa, central area of the tumor, and the invasive front of the same tumor is shown. ( A – C ) Cdc42 IHC ( A ), fascin IHC ( B ), and MYC IHC

    Article Snippet: Antibodies against c-MYC (9E10), Sin3, and fascin were from Santa Cruz Biotechnology.

    Techniques: Immunohistochemistry

    Analysis of fascin expression in MYC-nick induced cell migration. ( A ) Effect of MYC-nick on abundance of endogenous fascin and exogenous GFP-fascin. ( B ) Fascin expression in murine intestinal and colonic lesions derived from different genetic backgrounds

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: MYC-nick promotes cell migration by inducing fascin expression and Cdc42 activation

    doi: 10.1073/pnas.1610994113

    Figure Lengend Snippet: Analysis of fascin expression in MYC-nick induced cell migration. ( A ) Effect of MYC-nick on abundance of endogenous fascin and exogenous GFP-fascin. ( B ) Fascin expression in murine intestinal and colonic lesions derived from different genetic backgrounds

    Article Snippet: Antibodies against c-MYC (9E10), Sin3, and fascin were from Santa Cruz Biotechnology.

    Techniques: Expressing, Migration, Derivative Assay

    The fascin-1 protein expression status and progression-free survival of glioma patients.

    Journal: Open Medicine

    Article Title: High Expression Levels of Fascin-1 Protein in Human Gliomas and its Clinical Relevance

    doi: 10.1515/med-2018-0080

    Figure Lengend Snippet: The fascin-1 protein expression status and progression-free survival of glioma patients.

    Article Snippet: The staining procedures carried out included conventional deparaffinizing of paraffin sections, antigen retrieval, blocking of endogenous peroxidase with 3% hydrogen peroxide, blocking non-specific binding with goat serum, and incubation with human anti-fascin-1 monoclonal antibody (1:400; Santa Cruz Biotechnologies, Santa Cruz, CA, USA).

    Techniques: Expressing

    The fascin-1 mRNA was detected by RT-PCR. T: glioma tissue; N: normal brain tissue.

    Journal: Open Medicine

    Article Title: High Expression Levels of Fascin-1 Protein in Human Gliomas and its Clinical Relevance

    doi: 10.1515/med-2018-0080

    Figure Lengend Snippet: The fascin-1 mRNA was detected by RT-PCR. T: glioma tissue; N: normal brain tissue.

    Article Snippet: The staining procedures carried out included conventional deparaffinizing of paraffin sections, antigen retrieval, blocking of endogenous peroxidase with 3% hydrogen peroxide, blocking non-specific binding with goat serum, and incubation with human anti-fascin-1 monoclonal antibody (1:400; Santa Cruz Biotechnologies, Santa Cruz, CA, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    The fascin-1 protein expression in glioma tissues (A and B) and normal brain tissue (C and D). (A and C) ×20; (B and D) ×50. (A and B) Male, 57 years old; (C and D) Female, 49 years old.

    Journal: Open Medicine

    Article Title: High Expression Levels of Fascin-1 Protein in Human Gliomas and its Clinical Relevance

    doi: 10.1515/med-2018-0080

    Figure Lengend Snippet: The fascin-1 protein expression in glioma tissues (A and B) and normal brain tissue (C and D). (A and C) ×20; (B and D) ×50. (A and B) Male, 57 years old; (C and D) Female, 49 years old.

    Article Snippet: The staining procedures carried out included conventional deparaffinizing of paraffin sections, antigen retrieval, blocking of endogenous peroxidase with 3% hydrogen peroxide, blocking non-specific binding with goat serum, and incubation with human anti-fascin-1 monoclonal antibody (1:400; Santa Cruz Biotechnologies, Santa Cruz, CA, USA).

    Techniques: Expressing

    Activation of Fas signaling upregulated Fascin expression in AGS cells through activation of STAT3. The AGS cells were stimulated with 5 μg/ml of anti-Fas in the indicated times. (A) The phosphorylated STAT3 was detected by Western blot. (B) The expression of Fascin mRNA was assayed by real-time PCR. (C) After stimulation with 5 μg/ml anti-Fas for 24 h, the protein level of phosphorylated STAT3 and Fascin in AGS cells was detected by Western blot. (D) The AGS cells were pre-treated with 10 μM of Stattic for 2 h and followed by 5 μg/ml of anti-Fas stimulation for 24 h; the protein level of phosphorylated STAT3 and Fascin was detected by Western blot. After transfection with STAT3 siRNA or NC siRNA for 36 h, (E) the STAT3 expression in the AGS cells was detected by Western blot; (F) the AGS cells were then stimulated with 5 μg/ml of anti-Fas for 2 h, and the Fascin expression in the cells was detected by Western blot. Data are representative of three independent experiments.

    Journal: PLoS ONE

    Article Title: Fas Signaling Promotes Gastric Cancer Metastasis through STAT3-Dependent Upregulation of Fascin

    doi: 10.1371/journal.pone.0125132

    Figure Lengend Snippet: Activation of Fas signaling upregulated Fascin expression in AGS cells through activation of STAT3. The AGS cells were stimulated with 5 μg/ml of anti-Fas in the indicated times. (A) The phosphorylated STAT3 was detected by Western blot. (B) The expression of Fascin mRNA was assayed by real-time PCR. (C) After stimulation with 5 μg/ml anti-Fas for 24 h, the protein level of phosphorylated STAT3 and Fascin in AGS cells was detected by Western blot. (D) The AGS cells were pre-treated with 10 μM of Stattic for 2 h and followed by 5 μg/ml of anti-Fas stimulation for 24 h; the protein level of phosphorylated STAT3 and Fascin was detected by Western blot. After transfection with STAT3 siRNA or NC siRNA for 36 h, (E) the STAT3 expression in the AGS cells was detected by Western blot; (F) the AGS cells were then stimulated with 5 μg/ml of anti-Fas for 2 h, and the Fascin expression in the cells was detected by Western blot. Data are representative of three independent experiments.

    Article Snippet: The mouse anti-human Fascin (D-10) monoclonal antibody, human Fascin siRNA, STAT3 siRNA, negative control siRNA (NC siRNA), and STAT3 inhibitor, Stattic, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Activation Assay, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Transfection

    Fas signaling promoted AGS cell migration dependent on STAT3/Fascin pathway. (A) AGS cells were transfected with Fascin siRNA or NC siRNA for 36 h, and Fascin expression in the cells was detected by Western blot. After (B) inhibition of Fascin expression by siRNA; or (C) treated with 10 μM Stattic for 2 h; or (D) inhibition of STAT3 expression by siRNA, and stimulated with 5 μg/ml of anti-Fas for 2 h, the number of AGS cells which migrated to the bottom of the Transwell filter was quantified (n = 5). Data are representative of three independent experiments. (**p

    Journal: PLoS ONE

    Article Title: Fas Signaling Promotes Gastric Cancer Metastasis through STAT3-Dependent Upregulation of Fascin

    doi: 10.1371/journal.pone.0125132

    Figure Lengend Snippet: Fas signaling promoted AGS cell migration dependent on STAT3/Fascin pathway. (A) AGS cells were transfected with Fascin siRNA or NC siRNA for 36 h, and Fascin expression in the cells was detected by Western blot. After (B) inhibition of Fascin expression by siRNA; or (C) treated with 10 μM Stattic for 2 h; or (D) inhibition of STAT3 expression by siRNA, and stimulated with 5 μg/ml of anti-Fas for 2 h, the number of AGS cells which migrated to the bottom of the Transwell filter was quantified (n = 5). Data are representative of three independent experiments. (**p

    Article Snippet: The mouse anti-human Fascin (D-10) monoclonal antibody, human Fascin siRNA, STAT3 siRNA, negative control siRNA (NC siRNA), and STAT3 inhibitor, Stattic, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Migration, Transfection, Expressing, Western Blot, Inhibition