Structured Review

Epitomics fascin
Fascin, supplied by Epitomics, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fascin/product/Epitomics
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fascin - by Bioz Stars, 2020-07
91/100 stars

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Article Title: Fascin expression is inversely correlated with breast cancer metastasis suppressor 1 and predicts a worse survival outcome in node-negative breast cancer patients
Article Snippet: .. Immunohistochemical staining and interpretation Immunohistochemistry (IHC) staining was performed on the TMA blocks using a mouse monoclonal antibody to fascin (Epitomics, Burlingame, CA, USA; 1:100) and rabbit polyclonal antibody to BRMS1 (Genetex, Irvine, CA, USA; 1:100). .. Briefly, 4 μm TMA sections were transferred to adhesive slides and dried at 62°C for 30 min. After incubation with primary antibodies, immunodetection was achieved with the addition of biotinylated anti-mouse and rabbit immunoglobulins, followed by peroxidase-labeled streptavidin (a component of a streptavidin-biotin kit), and 3,3'-diaminobenzidine as the chromogenic substrate.

Immunohistochemistry:

Article Title: Fascin expression is inversely correlated with breast cancer metastasis suppressor 1 and predicts a worse survival outcome in node-negative breast cancer patients
Article Snippet: .. Immunohistochemical staining and interpretation Immunohistochemistry (IHC) staining was performed on the TMA blocks using a mouse monoclonal antibody to fascin (Epitomics, Burlingame, CA, USA; 1:100) and rabbit polyclonal antibody to BRMS1 (Genetex, Irvine, CA, USA; 1:100). .. Briefly, 4 μm TMA sections were transferred to adhesive slides and dried at 62°C for 30 min. After incubation with primary antibodies, immunodetection was achieved with the addition of biotinylated anti-mouse and rabbit immunoglobulins, followed by peroxidase-labeled streptavidin (a component of a streptavidin-biotin kit), and 3,3'-diaminobenzidine as the chromogenic substrate.

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    Epitomics human fascin1
    The inactivation of <t>fascin1</t> inhibits proliferation and invasive ability of ovarian cancer cell lines SKOV3, and OVCAR3. (A) Cell proliferation was measured by colony forming assay after fascin1 siRNA transfection. The colony formation of the transfected cells grew significantly slower than control cells at 72 h. Colony numbers of transfected cells decreased significantly to 95.7% (SKOV3), 78.1% (OVCAR3) compared with that in control cells (72 h) (P
    Human Fascin1, supplied by Epitomics, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fascin1/product/Epitomics
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human fascin1 - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    91
    Epitomics fascin
    The inactivation of <t>fascin1</t> inhibits proliferation and invasive ability of ovarian cancer cell lines SKOV3, and OVCAR3. (A) Cell proliferation was measured by colony forming assay after fascin1 siRNA transfection. The colony formation of the transfected cells grew significantly slower than control cells at 72 h. Colony numbers of transfected cells decreased significantly to 95.7% (SKOV3), 78.1% (OVCAR3) compared with that in control cells (72 h) (P
    Fascin, supplied by Epitomics, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fascin/product/Epitomics
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fascin - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    The inactivation of fascin1 inhibits proliferation and invasive ability of ovarian cancer cell lines SKOV3, and OVCAR3. (A) Cell proliferation was measured by colony forming assay after fascin1 siRNA transfection. The colony formation of the transfected cells grew significantly slower than control cells at 72 h. Colony numbers of transfected cells decreased significantly to 95.7% (SKOV3), 78.1% (OVCAR3) compared with that in control cells (72 h) (P

    Journal: International Journal of Oncology

    Article Title: Fascin1 expression in high-grade serous ovarian carcinoma is a prognostic marker and knockdown of fascin1 suppresses the proliferation of ovarian cancer cells

    doi: 10.3892/ijo.2013.2232

    Figure Lengend Snippet: The inactivation of fascin1 inhibits proliferation and invasive ability of ovarian cancer cell lines SKOV3, and OVCAR3. (A) Cell proliferation was measured by colony forming assay after fascin1 siRNA transfection. The colony formation of the transfected cells grew significantly slower than control cells at 72 h. Colony numbers of transfected cells decreased significantly to 95.7% (SKOV3), 78.1% (OVCAR3) compared with that in control cells (72 h) (P

    Article Snippet: After three rinses in phosphate-buffered saline (PBS) for 5 min, each section was incubated for 1 h at room temperature with mouse monoclonal antibodies to human fascin1 (Epitomics, Burlingame, CA, USA; 1:100), and human β-catenin (Novus Biologicals, Littleton, CO, USA) all diluted in PBS; washed three times in PBS for 5 min; incubated with horseradish peroxidase-labeled rabbit anti-mouse immunoglobulin (DAKO; 1 h at room temperature); washed three times; and incubated with a solution of diaminobenzidine (DAB) at room temperature to visualize peroxidase activity.

    Techniques: Transfection

    Fascin1 expression after siRNA transfection. (A) Real-time PCR analysis revealed the fascin1 expression was inhibited in the cancer cells transfected with fascin1 siRNA compared with control cancer cells (P

    Journal: International Journal of Oncology

    Article Title: Fascin1 expression in high-grade serous ovarian carcinoma is a prognostic marker and knockdown of fascin1 suppresses the proliferation of ovarian cancer cells

    doi: 10.3892/ijo.2013.2232

    Figure Lengend Snippet: Fascin1 expression after siRNA transfection. (A) Real-time PCR analysis revealed the fascin1 expression was inhibited in the cancer cells transfected with fascin1 siRNA compared with control cancer cells (P

    Article Snippet: After three rinses in phosphate-buffered saline (PBS) for 5 min, each section was incubated for 1 h at room temperature with mouse monoclonal antibodies to human fascin1 (Epitomics, Burlingame, CA, USA; 1:100), and human β-catenin (Novus Biologicals, Littleton, CO, USA) all diluted in PBS; washed three times in PBS for 5 min; incubated with horseradish peroxidase-labeled rabbit anti-mouse immunoglobulin (DAKO; 1 h at room temperature); washed three times; and incubated with a solution of diaminobenzidine (DAB) at room temperature to visualize peroxidase activity.

    Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction

    Kaplan-Meier survival analysis of progression-free survival in all patients according to fascin1 expression. Significant differences among the subgroups with positive (dimed line) and negative (bold line) fascin1 expression indicate poor outcomes in patients with fascin1 expression group. Fascin1 expression group was significantly correlated with shorter progression-free survival (P

    Journal: International Journal of Oncology

    Article Title: Fascin1 expression in high-grade serous ovarian carcinoma is a prognostic marker and knockdown of fascin1 suppresses the proliferation of ovarian cancer cells

    doi: 10.3892/ijo.2013.2232

    Figure Lengend Snippet: Kaplan-Meier survival analysis of progression-free survival in all patients according to fascin1 expression. Significant differences among the subgroups with positive (dimed line) and negative (bold line) fascin1 expression indicate poor outcomes in patients with fascin1 expression group. Fascin1 expression group was significantly correlated with shorter progression-free survival (P

    Article Snippet: After three rinses in phosphate-buffered saline (PBS) for 5 min, each section was incubated for 1 h at room temperature with mouse monoclonal antibodies to human fascin1 (Epitomics, Burlingame, CA, USA; 1:100), and human β-catenin (Novus Biologicals, Littleton, CO, USA) all diluted in PBS; washed three times in PBS for 5 min; incubated with horseradish peroxidase-labeled rabbit anti-mouse immunoglobulin (DAKO; 1 h at room temperature); washed three times; and incubated with a solution of diaminobenzidine (DAB) at room temperature to visualize peroxidase activity.

    Techniques: Expressing

    Representative immunohistochemical analysis of the fascin1 and β-catenin expression. (A) Fascin1 was negative in the epithelial cells of the fallopian tube (negative control) and immunopositive in the endothelial cells (positive control) and (B) positive staining in cytoplasm of tumor cells of the high-grade ovarian serous carcinoma. β-catenin was observed clear membrane staining in all the cases of (C) fallopian tube and of (D) high-grade ovarian serous carcinoma.

    Journal: International Journal of Oncology

    Article Title: Fascin1 expression in high-grade serous ovarian carcinoma is a prognostic marker and knockdown of fascin1 suppresses the proliferation of ovarian cancer cells

    doi: 10.3892/ijo.2013.2232

    Figure Lengend Snippet: Representative immunohistochemical analysis of the fascin1 and β-catenin expression. (A) Fascin1 was negative in the epithelial cells of the fallopian tube (negative control) and immunopositive in the endothelial cells (positive control) and (B) positive staining in cytoplasm of tumor cells of the high-grade ovarian serous carcinoma. β-catenin was observed clear membrane staining in all the cases of (C) fallopian tube and of (D) high-grade ovarian serous carcinoma.

    Article Snippet: After three rinses in phosphate-buffered saline (PBS) for 5 min, each section was incubated for 1 h at room temperature with mouse monoclonal antibodies to human fascin1 (Epitomics, Burlingame, CA, USA; 1:100), and human β-catenin (Novus Biologicals, Littleton, CO, USA) all diluted in PBS; washed three times in PBS for 5 min; incubated with horseradish peroxidase-labeled rabbit anti-mouse immunoglobulin (DAKO; 1 h at room temperature); washed three times; and incubated with a solution of diaminobenzidine (DAB) at room temperature to visualize peroxidase activity.

    Techniques: Immunohistochemistry, Expressing, Negative Control, Positive Control, Staining