fasciculin ii (Alomone Labs)


Structured Review

Fasciculin Ii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fasciculin ii/product/Alomone Labs
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Arhgef5 Binds α-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity"
Article Title: Arhgef5 Binds α-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity
Journal: Frontiers in Molecular Neuroscience
doi: 10.3389/fnmol.2020.00104

Figure Legend Snippet: Generation of muscle-specific Arhgef5 KO mice. (A) Strategy for the generation of conditional Arhgef5 knockouts. Mice with loxP sites flanking the third exon of Arhgef5 were crossed with Cre-recombinase expressing mice to produce knockout. (B) Genotyping shows successful recombination in the muscles but not tail tipss of AG5 fl/fl ; Acta-Cre mice. (C) Quantification of Arhgef5 mRNA expression in AG5 fl/fl and AG5 fl/fl ; Acta-Cre mice at 50 and 500 days of age. Results are mean ± SEM of three mice from each genotype. (D) Proper alignment of pre- and post-synaptic elements in AG5 fl/fl ; Acta-Cre muscles. Single fibers of tibialis anterior muscles isolated from AG5 fl/fl and AG5 fl/fl ; Acta-Cre mice were stained with BTX (to visualize AChR), fasciculin II (to visualize the synaptic cleft marker acetylcholinesterase), and anti-neurofilament + anti-synaptophysin antibodies (to visualize the presynaptic nerve terminal). Scale bar is 20 μm. ∗ p
Techniques Used: Mouse Assay, Expressing, Knock-Out, Isolation, Staining, Marker