Journal: Microbiology Spectrum
Article Title: Characterization of vaginal microbiomes in clinician-collected bacterial vaginosis diagnosed samples
doi: 10.1128/spectrum.02582-24
Figure Lengend Snippet: 16S V3–V4 rRNA sequencing of remnant clinician-collected vaginal swabs previously analyzed via the Labcorp NuSwab BV PCR test. ( a ) Example process of determining the bacterial vaginosis (BV) status of a vaginal swab using the NuSwab three-microbe panel composed of Atopobium vaginae , BVAB-2, and Megasphaera-1 . Each microbe is quantified and given a score of 0 (low), 1 (moderate), or 2 (high). The total score is then interpreted as BV-POS (3–6), BV-IND (2), or BV-NEG (0–1). ( b ) 16S relative abundances of NuSwab panel microbes stratified by their scores. Statistical comparisons of 16S relative abundances were made between samples with NuSwab scores of 2 and those with scores of 0 or 1 using Wilcoxon rank-sum tests with FDR control.
Article Snippet: Modern PCR-based clinical tests target panels of BV-associated microbes, such as the Labcorp NuSwab test that targets Atopobium ( Fannyhessea ) vaginae , Megasphaera-1 , and Bacterial Vaginosis Associated Bacterium (BVAB)−2 .
Techniques: Sequencing, Control