fam labelled probes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fam labelled probes
    Fam Labelled Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fam labelled probes/product/Thermo Fisher
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    fam labelled probes - by Bioz Stars, 2020-12
    99/100 stars

    Images

    Related Articles

    Optical Imaging:

    Article Title: Progesterone Potentiates Calcium Release Through IP3 Receptor by an Akt-Mediated Mechanism in Hippocampal Neurons
    Article Snippet: .. Optical imaging of [Ca2+ ]i After 11 DIV, mouse hippocampal cells were incubated with the Ca2+ indicator dye, 4μM Fluo-3 acetoxymethylester (Fluo-3-AM, Molecular Probes) in extracellular solution (ECS, in mM: NaCl, 137; KCl, 5; CaCl2 2; Na2 HPO4 1; MgSO4 1; HEPES, 10; glucose, 22; pH 7.4) at 37°C for 30 min and then rinsed with ECS. .. Subsequently, we measured changes in fluorescence intensity of fluo-3 AM in loaded cells using time-lapse videomicroscopy (model IX70; Olympus, Japan; ORCA-ER, a high-resolution 12-bit digital B/W cooled CCD camera, Hamamatsu, Japan; Lambda DG-4 ultra high speed wavelength switcher; Sutter Instrument Co., Novato, CA; Simple PCI Imaging Software, Ver.

    Incubation:

    Article Title: Progesterone Potentiates Calcium Release Through IP3 Receptor by an Akt-Mediated Mechanism in Hippocampal Neurons
    Article Snippet: .. Optical imaging of [Ca2+ ]i After 11 DIV, mouse hippocampal cells were incubated with the Ca2+ indicator dye, 4μM Fluo-3 acetoxymethylester (Fluo-3-AM, Molecular Probes) in extracellular solution (ECS, in mM: NaCl, 137; KCl, 5; CaCl2 2; Na2 HPO4 1; MgSO4 1; HEPES, 10; glucose, 22; pH 7.4) at 37°C for 30 min and then rinsed with ECS. .. Subsequently, we measured changes in fluorescence intensity of fluo-3 AM in loaded cells using time-lapse videomicroscopy (model IX70; Olympus, Japan; ORCA-ER, a high-resolution 12-bit digital B/W cooled CCD camera, Hamamatsu, Japan; Lambda DG-4 ultra high speed wavelength switcher; Sutter Instrument Co., Novato, CA; Simple PCI Imaging Software, Ver.

    Binding Assay:

    Article Title: ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression
    Article Snippet: .. Dil-Ox-LDL uptake and binding assays DiI-Ox-LDL lipoproteins were oxidized and labelled with the fluorescent probe DiI (Molecular Probes, Invitrogen). .. For the uptake assays, mouse peritoneal macrophages were washed once in 1X PBS and incubated in fresh media containing DiI-Ox-LDL (30 μg cholesterol per ml) for 2 h at 37 °C.

    Transfection:

    Article Title: HIV-1 Nef Interacts with Inositol Trisphosphate Receptor to Activate Calcium Signaling in T Cells
    Article Snippet: .. Bound cells (75–90% of which were transfection-positive as determined by parallel green fluorescent protein (GFP)/CD8 transfection controls) were collected by pipetting, washed twice with 800 μl of RPMI-1640 (without phenol red; Hyclone), and loaded with 3 μM Fluo-3- and 5 μM Fura-Red-acetomethylesters in the presence of 0.02% Pluronic F-127 (all three from Molecular Probes) for 20 min at RT. .. Cells were washed twice with RPMI-1640 (1% serum, without phenol red) and suspended into 1 ml of this medium followed by an incubation at +37°C, 5% CO2 for 20 min.

    Inverted Microscopy:

    Article Title: Melatonin Attenuates Cardiac Reperfusion Stress by Improving OPA1-Related Mitochondrial Fusion in a Yap–Hippo Pathway–Dependent Manner
    Article Snippet: .. Fluo-2-AM (Molecular Probes) was used to label intracellular calcium, and then cellular calcium mapping was performed under an inverted microscope. ..

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  • 96
    Thermo Fisher gene exp camp hs00189038 m1
    Comparison of iSS-predicted effects of 25OHD or 1,25(OH) 2 D on monocyte expression of <t>CAMP</t> with observed in vitro responses of monocytes to treatment with these metabolites. Adherent human monocytes were incubated for 6 hrs in media containing 5% serum with doses of (A) 25OHD (1–300 nM) and (B) 1,25(OH) 2 D (0.1–6 nM). The experimental data is indicated by blue dots and error bars (± SD). Black lines indicate data predicted by the iSS mathematical model assuming basal levels of VDR and CYP27B1 (i.e. no activation). For the purpose of this modeling, DBP was represented by the GC1F/1F allelic combination.
    Gene Exp Camp Hs00189038 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp camp hs00189038 m1/product/Thermo Fisher
    Average 96 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    gene exp camp hs00189038 m1 - by Bioz Stars, 2020-12
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    99
    Thermo Fisher gene exp myc hs00153408 m1
    ERK inhibition and KRAS silencing impairs mitochondrial function in PDAC cells. a , Cells were treated with vehicle (DMSO) or SCH772984 (ERKi, 1 μM) for 24 h. Shown is quantification of mean fluorescence of MitoTracker green staining, normalized to DMSO for each cell line across three independent experiments. P values are from two-sided, unpaired t -test; error bars denote s.e.m. b , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, anti-TOMM20; blue, DAPI. Scale bar, 20 μm. c , Quantitation of mitochondrial morphologies observed in cells shown in b . Some 50 cells per condition per repetition were blindly scored and data are averages of four independent experiments; error bars denote s.e.m. d , Pa14C cells were treated with SCH772984 (ERKi, 1 µM) for 1 h. Immunoblot of phosphorylated DRP1 (S616) (pDRP1), total DRP1 and β-actin is representative of three independent experiments. e , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, Anti-TOMM20; blue, DAPI; scale bar, 20 μm. f , Quantitation of mitochondrial morphologies observed in cells shown in e . Some 50 cells per condition per repetition were blindly scored; data are means of four independent experiments; error bars denote s.e.m. g , PDAC cells were treated with SCH772984 (ERKi, 1 μM) for 24 h. Immunoblot of pDRP1, total DRP1, <t>MYC,</t> phosphorylated RSK (pRSK) and β-actin is representative of three independent experiments. h , Cell lines derived from the iKRAS PDAC mouse model were deprived of doxycycline (Dox) to turn off Kras G12D for 24 h. Representative transmission electron microscopy (TEM) micrographs displaying mitochondrial morphologies in the presence and absence of doxycycline; images are representative of six independent experiments; scale bar, 500 nm. i , Cells were treated as described in a . Quantification of relative mitochondrial potential via labeling with the JC-1 mitochondrial dye. Data are the mean of three independent experiments; P values are from two-sided, unpaired t -test; error bars denote s.d. of median ratios of JC-1 aggregate fluorescence to JC-1 monomer fluorescence. j , Representative histograms from Pa01C and Pa14C cells quantified in i ; as a control, the uncoupler CCCP was added to acquired DMSO samples to establish minimum potential. k , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 1.5 h; shown is mean OCR of three independent experiments; error bars denote s.e.m. of mean OCR across replicates. l , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 24 h. Data for HPAC and Pa01C lines are the mean of nine independent experiments; data for Pa14C are the mean of eight independent experiments, and data for PANC-1 are the mean of three independent experiments. P values are from two-sided, unpaired t -test, comparing treatment conditions to DMSO, which was normalized to 100 for each measurement; error bars denote s.e.m.
    Gene Exp Myc Hs00153408 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp myc hs00153408 m1/product/Thermo Fisher
    Average 99 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher gene exp irf7 mm00516788 m1
    Gene and protein expression of plasma membrane and intracellular localized TLRs and of the IFN regulatory factors <t>IRF3</t> and <t>IRF7</t> in murine and human cardiac tissue under basal conditions. Cardiac tissue of healthy C57BL/6J wildtype mice was used for TaqMan based gene expression analysis of various TLRs and their IFN regulatory factors Irf3 and Irf7 under basal conditions. Gene expressions of Tlr4 , Tlr9 , Irf3 and Irf7 were highly expressed when compared to the remaining TLRs under basal conditions in murine cardiac tissue. The highest gene expression under basal condition was detected for Irf7 . In addition, we detected similar human protein expression patterns when compared with murine gene expression in cardiac tissue under basal conditions. Data are presented in box plots as absolute mRNA expression normalized to the house keeping gene Cdkn1b and human protein abundance are presented as rhombus sign on the right-hand of each mRNA expression.
    Gene Exp Irf7 Mm00516788 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp irf7 mm00516788 m1/product/Thermo Fisher
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Comparison of iSS-predicted effects of 25OHD or 1,25(OH) 2 D on monocyte expression of CAMP with observed in vitro responses of monocytes to treatment with these metabolites. Adherent human monocytes were incubated for 6 hrs in media containing 5% serum with doses of (A) 25OHD (1–300 nM) and (B) 1,25(OH) 2 D (0.1–6 nM). The experimental data is indicated by blue dots and error bars (± SD). Black lines indicate data predicted by the iSS mathematical model assuming basal levels of VDR and CYP27B1 (i.e. no activation). For the purpose of this modeling, DBP was represented by the GC1F/1F allelic combination.

    Journal: PLoS ONE

    Article Title: Vitamin D Binding Protein and Monocyte Response to 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D: Analysis by Mathematical Modeling

    doi: 10.1371/journal.pone.0030773

    Figure Lengend Snippet: Comparison of iSS-predicted effects of 25OHD or 1,25(OH) 2 D on monocyte expression of CAMP with observed in vitro responses of monocytes to treatment with these metabolites. Adherent human monocytes were incubated for 6 hrs in media containing 5% serum with doses of (A) 25OHD (1–300 nM) and (B) 1,25(OH) 2 D (0.1–6 nM). The experimental data is indicated by blue dots and error bars (± SD). Black lines indicate data predicted by the iSS mathematical model assuming basal levels of VDR and CYP27B1 (i.e. no activation). For the purpose of this modeling, DBP was represented by the GC1F/1F allelic combination.

    Article Snippet: Specifically, we utilized FAM-labeled TaqMan Gene Expression Assay probe/primer Hs00189038_m1 (CAMP) in conjunction with VIC/MGB Probe/Primer Eukaryotic 18S rRNA Endogenous Control (part number 4319413E) as the internal calibrator.

    Techniques: Expressing, In Vitro, Incubation, Activation Assay

    Schematic framework of parameters used to produce extracellular steady state (eSS) and intracellular (iSS) mathematical models for vitamin D metabolism and function. Free 25OHD and 1,25(OH) 2 D interacting with extra-cellular vitamin D binding protein (DBP) or albumin indicated in black text and arrows (eSS model). Intra-cellular interactions involving the vitamin D-activating enzyme (CYP27B1), the vitamin D receptor (VDR) and transcriptional induction of the antibacterial protein CAMP via interaction between VDR and the CAMP gene promoter (CAMP-DNA) indicated by grey text and arrows (iSS model).

    Journal: PLoS ONE

    Article Title: Vitamin D Binding Protein and Monocyte Response to 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D: Analysis by Mathematical Modeling

    doi: 10.1371/journal.pone.0030773

    Figure Lengend Snippet: Schematic framework of parameters used to produce extracellular steady state (eSS) and intracellular (iSS) mathematical models for vitamin D metabolism and function. Free 25OHD and 1,25(OH) 2 D interacting with extra-cellular vitamin D binding protein (DBP) or albumin indicated in black text and arrows (eSS model). Intra-cellular interactions involving the vitamin D-activating enzyme (CYP27B1), the vitamin D receptor (VDR) and transcriptional induction of the antibacterial protein CAMP via interaction between VDR and the CAMP gene promoter (CAMP-DNA) indicated by grey text and arrows (iSS model).

    Article Snippet: Specifically, we utilized FAM-labeled TaqMan Gene Expression Assay probe/primer Hs00189038_m1 (CAMP) in conjunction with VIC/MGB Probe/Primer Eukaryotic 18S rRNA Endogenous Control (part number 4319413E) as the internal calibrator.

    Techniques: Binding Assay

    ERK inhibition and KRAS silencing impairs mitochondrial function in PDAC cells. a , Cells were treated with vehicle (DMSO) or SCH772984 (ERKi, 1 μM) for 24 h. Shown is quantification of mean fluorescence of MitoTracker green staining, normalized to DMSO for each cell line across three independent experiments. P values are from two-sided, unpaired t -test; error bars denote s.e.m. b , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, anti-TOMM20; blue, DAPI. Scale bar, 20 μm. c , Quantitation of mitochondrial morphologies observed in cells shown in b . Some 50 cells per condition per repetition were blindly scored and data are averages of four independent experiments; error bars denote s.e.m. d , Pa14C cells were treated with SCH772984 (ERKi, 1 µM) for 1 h. Immunoblot of phosphorylated DRP1 (S616) (pDRP1), total DRP1 and β-actin is representative of three independent experiments. e , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, Anti-TOMM20; blue, DAPI; scale bar, 20 μm. f , Quantitation of mitochondrial morphologies observed in cells shown in e . Some 50 cells per condition per repetition were blindly scored; data are means of four independent experiments; error bars denote s.e.m. g , PDAC cells were treated with SCH772984 (ERKi, 1 μM) for 24 h. Immunoblot of pDRP1, total DRP1, MYC, phosphorylated RSK (pRSK) and β-actin is representative of three independent experiments. h , Cell lines derived from the iKRAS PDAC mouse model were deprived of doxycycline (Dox) to turn off Kras G12D for 24 h. Representative transmission electron microscopy (TEM) micrographs displaying mitochondrial morphologies in the presence and absence of doxycycline; images are representative of six independent experiments; scale bar, 500 nm. i , Cells were treated as described in a . Quantification of relative mitochondrial potential via labeling with the JC-1 mitochondrial dye. Data are the mean of three independent experiments; P values are from two-sided, unpaired t -test; error bars denote s.d. of median ratios of JC-1 aggregate fluorescence to JC-1 monomer fluorescence. j , Representative histograms from Pa01C and Pa14C cells quantified in i ; as a control, the uncoupler CCCP was added to acquired DMSO samples to establish minimum potential. k , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 1.5 h; shown is mean OCR of three independent experiments; error bars denote s.e.m. of mean OCR across replicates. l , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 24 h. Data for HPAC and Pa01C lines are the mean of nine independent experiments; data for Pa14C are the mean of eight independent experiments, and data for PANC-1 are the mean of three independent experiments. P values are from two-sided, unpaired t -test, comparing treatment conditions to DMSO, which was normalized to 100 for each measurement; error bars denote s.e.m.

    Journal: Nature medicine

    Article Title: Combination of ERK and autophagy inhibition as a treatment approach for pancreatic cancer

    doi: 10.1038/s41591-019-0368-8

    Figure Lengend Snippet: ERK inhibition and KRAS silencing impairs mitochondrial function in PDAC cells. a , Cells were treated with vehicle (DMSO) or SCH772984 (ERKi, 1 μM) for 24 h. Shown is quantification of mean fluorescence of MitoTracker green staining, normalized to DMSO for each cell line across three independent experiments. P values are from two-sided, unpaired t -test; error bars denote s.e.m. b , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, anti-TOMM20; blue, DAPI. Scale bar, 20 μm. c , Quantitation of mitochondrial morphologies observed in cells shown in b . Some 50 cells per condition per repetition were blindly scored and data are averages of four independent experiments; error bars denote s.e.m. d , Pa14C cells were treated with SCH772984 (ERKi, 1 µM) for 1 h. Immunoblot of phosphorylated DRP1 (S616) (pDRP1), total DRP1 and β-actin is representative of three independent experiments. e , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, Anti-TOMM20; blue, DAPI; scale bar, 20 μm. f , Quantitation of mitochondrial morphologies observed in cells shown in e . Some 50 cells per condition per repetition were blindly scored; data are means of four independent experiments; error bars denote s.e.m. g , PDAC cells were treated with SCH772984 (ERKi, 1 μM) for 24 h. Immunoblot of pDRP1, total DRP1, MYC, phosphorylated RSK (pRSK) and β-actin is representative of three independent experiments. h , Cell lines derived from the iKRAS PDAC mouse model were deprived of doxycycline (Dox) to turn off Kras G12D for 24 h. Representative transmission electron microscopy (TEM) micrographs displaying mitochondrial morphologies in the presence and absence of doxycycline; images are representative of six independent experiments; scale bar, 500 nm. i , Cells were treated as described in a . Quantification of relative mitochondrial potential via labeling with the JC-1 mitochondrial dye. Data are the mean of three independent experiments; P values are from two-sided, unpaired t -test; error bars denote s.d. of median ratios of JC-1 aggregate fluorescence to JC-1 monomer fluorescence. j , Representative histograms from Pa01C and Pa14C cells quantified in i ; as a control, the uncoupler CCCP was added to acquired DMSO samples to establish minimum potential. k , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 1.5 h; shown is mean OCR of three independent experiments; error bars denote s.e.m. of mean OCR across replicates. l , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 24 h. Data for HPAC and Pa01C lines are the mean of nine independent experiments; data for Pa14C are the mean of eight independent experiments, and data for PANC-1 are the mean of three independent experiments. P values are from two-sided, unpaired t -test, comparing treatment conditions to DMSO, which was normalized to 100 for each measurement; error bars denote s.e.m.

    Article Snippet: Real-time quantitative Taqman PCR was performed on the QuantStudio 6 Flex (Thermo Fisher) with minor groove binder FAM dye-labeled probes against MYC (Hs00153408_m1, Thermo Fisher), SQSTM1 (Hs1061917_g1, Thermo Fisher), GABARAPL1 (Hs00740588_mH, Thermo Fisher), WIPI1 (Hs00924447_m1, Thermo Fisher), BECN1 (Hs01007018_m1, Thermo Fisher) and PRKAA1 (Hs01562315_m1, Thermo Fisher) and endogenous control VIC and TAMRA dye-labeled ACTB (Thermo Fisher).

    Techniques: Inhibition, Fluorescence, Staining, Quantitation Assay, Derivative Assay, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Labeling

    Gene and protein expression of plasma membrane and intracellular localized TLRs and of the IFN regulatory factors IRF3 and IRF7 in murine and human cardiac tissue under basal conditions. Cardiac tissue of healthy C57BL/6J wildtype mice was used for TaqMan based gene expression analysis of various TLRs and their IFN regulatory factors Irf3 and Irf7 under basal conditions. Gene expressions of Tlr4 , Tlr9 , Irf3 and Irf7 were highly expressed when compared to the remaining TLRs under basal conditions in murine cardiac tissue. The highest gene expression under basal condition was detected for Irf7 . In addition, we detected similar human protein expression patterns when compared with murine gene expression in cardiac tissue under basal conditions. Data are presented in box plots as absolute mRNA expression normalized to the house keeping gene Cdkn1b and human protein abundance are presented as rhombus sign on the right-hand of each mRNA expression.

    Journal: PLoS ONE

    Article Title: Role of Toll-like receptors and interferon regulatory factors in different experimental heart failure models of diverse etiology: IRF7 as novel cardiovascular stress-inducible factor

    doi: 10.1371/journal.pone.0193844

    Figure Lengend Snippet: Gene and protein expression of plasma membrane and intracellular localized TLRs and of the IFN regulatory factors IRF3 and IRF7 in murine and human cardiac tissue under basal conditions. Cardiac tissue of healthy C57BL/6J wildtype mice was used for TaqMan based gene expression analysis of various TLRs and their IFN regulatory factors Irf3 and Irf7 under basal conditions. Gene expressions of Tlr4 , Tlr9 , Irf3 and Irf7 were highly expressed when compared to the remaining TLRs under basal conditions in murine cardiac tissue. The highest gene expression under basal condition was detected for Irf7 . In addition, we detected similar human protein expression patterns when compared with murine gene expression in cardiac tissue under basal conditions. Data are presented in box plots as absolute mRNA expression normalized to the house keeping gene Cdkn1b and human protein abundance are presented as rhombus sign on the right-hand of each mRNA expression.

    Article Snippet: To assess the mRNA expression of the target genes real time PCR was performed using 5 μl of the gene expression master mix (life technologies) and the 0.5 μl of the gene expression assay for Tlr1 (Mm00446095_m1), Tlr2 (Mm00442346_m1), Tlr3 (Mm01207404_m1), Tlr4 (Mm00445273_m1), Tlr5 (Mm00546288_m1), Tlr6 (Mm02529782_s1), Tlr7 (Mm00446917_m1), Tlr8 (Mm04209873_m1), Tlr9 (mM00446193_m1), Irf3 (Mm00516784_m1), and Irf7 (Mm00516788_m1) (each includes forward and reverse primers as well the fluorescently FAM-labeled probe).

    Techniques: Expressing, Mouse Assay

    Gene expression levels of the IFN regulatory factors Irf3 and Irf7 in different HF models of diverse etiology. Cardiac tissue of healthy and diseased C57BL/6J mice was used for TaqMan based gene expression analysis of the IFN regulatory factors Irf3 and Irf7 . Expression levels of cardiac tissue from control mice are shown as white boxes, from diseased animals as red, blue, green or yellow boxes corresponding to the analyzed heart failure model. In viral-induced myocarditis (shown in red), gene expression of Irf7 showed by far the highest increase 7 days after infection compared to healthy controls. However, Irf3 displayed no changes in gene expression during viral myocarditis. Moreover, in the model of myocardial infarction (shown in blue), gene expression of Irf3 and Irf7 was highly increased 5 days post infarction in the scar tissue when compared to the non-infarcted LV or sham, whereas Irf7 showed a clearly higher gene expression when compared to the expression of Irf3 . In STZ-induced diabetic cardiomyopathy (shown in green), an opposite effect on gene expression of Irf3 and Irf7 was observed. Whereas, Irf3 showed a decreased gene expression, Irf7 displayed an increased gene expression under diabetic conditions. However, in the heart failure model caused by chronic AngII-infusion for 21 days (shown in yellow), no differences in gene expression of Irf3 and Irf7 were detected. Data are presented in box plots as relative mRNA expression in fold change to the corresponding untreated control using the formula 2 −ΔΔCt . * = significantly different compared to corresponding control; # = significantly different compared to VM (acute—7 days) or RZ (remote zone).

    Journal: PLoS ONE

    Article Title: Role of Toll-like receptors and interferon regulatory factors in different experimental heart failure models of diverse etiology: IRF7 as novel cardiovascular stress-inducible factor

    doi: 10.1371/journal.pone.0193844

    Figure Lengend Snippet: Gene expression levels of the IFN regulatory factors Irf3 and Irf7 in different HF models of diverse etiology. Cardiac tissue of healthy and diseased C57BL/6J mice was used for TaqMan based gene expression analysis of the IFN regulatory factors Irf3 and Irf7 . Expression levels of cardiac tissue from control mice are shown as white boxes, from diseased animals as red, blue, green or yellow boxes corresponding to the analyzed heart failure model. In viral-induced myocarditis (shown in red), gene expression of Irf7 showed by far the highest increase 7 days after infection compared to healthy controls. However, Irf3 displayed no changes in gene expression during viral myocarditis. Moreover, in the model of myocardial infarction (shown in blue), gene expression of Irf3 and Irf7 was highly increased 5 days post infarction in the scar tissue when compared to the non-infarcted LV or sham, whereas Irf7 showed a clearly higher gene expression when compared to the expression of Irf3 . In STZ-induced diabetic cardiomyopathy (shown in green), an opposite effect on gene expression of Irf3 and Irf7 was observed. Whereas, Irf3 showed a decreased gene expression, Irf7 displayed an increased gene expression under diabetic conditions. However, in the heart failure model caused by chronic AngII-infusion for 21 days (shown in yellow), no differences in gene expression of Irf3 and Irf7 were detected. Data are presented in box plots as relative mRNA expression in fold change to the corresponding untreated control using the formula 2 −ΔΔCt . * = significantly different compared to corresponding control; # = significantly different compared to VM (acute—7 days) or RZ (remote zone).

    Article Snippet: To assess the mRNA expression of the target genes real time PCR was performed using 5 μl of the gene expression master mix (life technologies) and the 0.5 μl of the gene expression assay for Tlr1 (Mm00446095_m1), Tlr2 (Mm00442346_m1), Tlr3 (Mm01207404_m1), Tlr4 (Mm00445273_m1), Tlr5 (Mm00546288_m1), Tlr6 (Mm02529782_s1), Tlr7 (Mm00446917_m1), Tlr8 (Mm04209873_m1), Tlr9 (mM00446193_m1), Irf3 (Mm00516784_m1), and Irf7 (Mm00516788_m1) (each includes forward and reverse primers as well the fluorescently FAM-labeled probe).

    Techniques: Expressing, Mouse Assay, Infection