fam labeled probes  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher fam labeled probes
    Fam Labeled Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fam labeled probes/product/Thermo Fisher
    Average 99 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    fam labeled probes - by Bioz Stars, 2020-12
    99/100 stars

    Images

    Related Articles

    Conjugation Assay:

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain
    Article Snippet: .. Tyramide synthesis To synthesize tyramide reagents [ ], the following succinimidyl esters were used for conjugation with tyramine (Sigma-Aldrich: St. Louis, MO, USA T2879): 5-(and-6)-carboxyfluorescein succinimidyl ester (FAM-SE; Molecular Probes C-1311), 5-(and-6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE; Molecular Probes C-1171) and DyLight 633 N-hydroxysuccinimide ester (DyLight633-SE; Pierce: Rockford, IL, USA 46414). .. Each succinimidyl ester was dissolved at a concentration of 10 mg/ml in dimethylformamide (Sigma-Aldrich D4551).

    Flow Cytometry:

    Article Title: Effects of Streptococcus pneumoniae Strain Background on Complement Resistance
    Article Snippet: .. Neutrophil association was investigated using an established flow cytometry assay, fresh human neutrophils (105 per reaction) and S. pneumoniae (106 per reaction) labelled with 6-carboxyfluorescein succinimidyl ester (FAMSE; Molecular Probes) and incubated in serum (diluted in PBS) for 20 min at 37°C , . ..

    Cytometry:

    Article Title: Effects of Streptococcus pneumoniae Strain Background on Complement Resistance
    Article Snippet: .. Neutrophil association was investigated using an established flow cytometry assay, fresh human neutrophils (105 per reaction) and S. pneumoniae (106 per reaction) labelled with 6-carboxyfluorescein succinimidyl ester (FAMSE; Molecular Probes) and incubated in serum (diluted in PBS) for 20 min at 37°C , . ..

    Labeling:

    Article Title: ASSEMBLY AND INTRACELLULAR TRAFFICKING OF HLA-B*3501 and HLA-B*3503
    Article Snippet: .. A fluorescent version of YPLHEQHGM was obtained by substituting the histidine residue at position 4 with a lysine residue labeled with 5-(and-6) carboxyfluorescein (C-1311) (Molecular Probes). .. The peptide was then synthesized using standard Fmoc Solid Phase Peptide Synthesis with the special lysine derivative incorporated and the labeled peptide purified using reverse phase HPLC.

    Article Title: Cancer causes metabolic perturbations associated with reduced insulin-stimulated glucose uptake in peripheral tissues and impaired muscle microvascular perfusion
    Article Snippet: .. All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP) which was 5′ FAM with minor groove binding. ..

    Microarray:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: .. To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ). .. Evaluation of the potential functional associations of these 219 probe sets using the Ingenuity Pathway Analysis program identified the glycerophospholipid metabolism pathway as the most significantly enriched, supporting in cancer cells our observations associating C1311 with lipid biogenesis in budding yeast.

    Incubation:

    Article Title: Effects of Streptococcus pneumoniae Strain Background on Complement Resistance
    Article Snippet: .. Neutrophil association was investigated using an established flow cytometry assay, fresh human neutrophils (105 per reaction) and S. pneumoniae (106 per reaction) labelled with 6-carboxyfluorescein succinimidyl ester (FAMSE; Molecular Probes) and incubated in serum (diluted in PBS) for 20 min at 37°C , . ..

    other:

    Article Title: The Amount of BCL6 in B Cells Shortly after Antigen Engagement Determines Their Representation in Subsequent Germinal Centers.
    Article Snippet: In-House Fluorophore Conjugations In house manufactured reagents were conjugated to fluorophores using 5-(and-6)-Carboxyfluorescein, Succinimidyl Ester (C1311; Thermo Fisher Scientific), Alexa Fluor 680 NHS Ester (A20008; Thermo Fisher Scientific) or (+)-Biotin N-hydroxysuccinimide ester (H1759; Sigma-Aldrich).

    Expressing:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: .. To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ). .. Evaluation of the potential functional associations of these 219 probe sets using the Ingenuity Pathway Analysis program identified the glycerophospholipid metabolism pathway as the most significantly enriched, supporting in cancer cells our observations associating C1311 with lipid biogenesis in budding yeast.

    Binding Assay:

    Article Title: Cancer causes metabolic perturbations associated with reduced insulin-stimulated glucose uptake in peripheral tissues and impaired muscle microvascular perfusion
    Article Snippet: .. All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP) which was 5′ FAM with minor groove binding. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Thermo Fisher gene exp camp hs00189038 m1
    Comparison of iSS-predicted effects of 25OHD or 1,25(OH) 2 D on monocyte expression of <t>CAMP</t> with observed in vitro responses of monocytes to treatment with these metabolites. Adherent human monocytes were incubated for 6 hrs in media containing 5% serum with doses of (A) 25OHD (1–300 nM) and (B) 1,25(OH) 2 D (0.1–6 nM). The experimental data is indicated by blue dots and error bars (± SD). Black lines indicate data predicted by the iSS mathematical model assuming basal levels of VDR and CYP27B1 (i.e. no activation). For the purpose of this modeling, DBP was represented by the GC1F/1F allelic combination.
    Gene Exp Camp Hs00189038 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp camp hs00189038 m1/product/Thermo Fisher
    Average 96 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    gene exp camp hs00189038 m1 - by Bioz Stars, 2020-12
    96/100 stars
      Buy from Supplier

    86
    Thermo Fisher copy number variation gpd1 hs00911535 cn
    Radio-pathologic and genetic findings in proband with <t>GPD1</t> mutation. ( a ) Liver biopsy: the liver parenchyma shows macro- and microvesicular fatty change on Hematoxylin and Eosin staining (H E), magnification × 10. ( b and c ) Ultrasound
    Copy Number Variation Gpd1 Hs00911535 Cn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/copy number variation gpd1 hs00911535 cn/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    copy number variation gpd1 hs00911535 cn - by Bioz Stars, 2020-12
    86/100 stars
      Buy from Supplier

    99
    Thermo Fisher gene exp myc hs00153408 m1
    ERK inhibition and KRAS silencing impairs mitochondrial function in PDAC cells. a , Cells were treated with vehicle (DMSO) or SCH772984 (ERKi, 1 μM) for 24 h. Shown is quantification of mean fluorescence of MitoTracker green staining, normalized to DMSO for each cell line across three independent experiments. P values are from two-sided, unpaired t -test; error bars denote s.e.m. b , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, anti-TOMM20; blue, DAPI. Scale bar, 20 μm. c , Quantitation of mitochondrial morphologies observed in cells shown in b . Some 50 cells per condition per repetition were blindly scored and data are averages of four independent experiments; error bars denote s.e.m. d , Pa14C cells were treated with SCH772984 (ERKi, 1 µM) for 1 h. Immunoblot of phosphorylated DRP1 (S616) (pDRP1), total DRP1 and β-actin is representative of three independent experiments. e , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, Anti-TOMM20; blue, DAPI; scale bar, 20 μm. f , Quantitation of mitochondrial morphologies observed in cells shown in e . Some 50 cells per condition per repetition were blindly scored; data are means of four independent experiments; error bars denote s.e.m. g , PDAC cells were treated with SCH772984 (ERKi, 1 μM) for 24 h. Immunoblot of pDRP1, total DRP1, <t>MYC,</t> phosphorylated RSK (pRSK) and β-actin is representative of three independent experiments. h , Cell lines derived from the iKRAS PDAC mouse model were deprived of doxycycline (Dox) to turn off Kras G12D for 24 h. Representative transmission electron microscopy (TEM) micrographs displaying mitochondrial morphologies in the presence and absence of doxycycline; images are representative of six independent experiments; scale bar, 500 nm. i , Cells were treated as described in a . Quantification of relative mitochondrial potential via labeling with the JC-1 mitochondrial dye. Data are the mean of three independent experiments; P values are from two-sided, unpaired t -test; error bars denote s.d. of median ratios of JC-1 aggregate fluorescence to JC-1 monomer fluorescence. j , Representative histograms from Pa01C and Pa14C cells quantified in i ; as a control, the uncoupler CCCP was added to acquired DMSO samples to establish minimum potential. k , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 1.5 h; shown is mean OCR of three independent experiments; error bars denote s.e.m. of mean OCR across replicates. l , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 24 h. Data for HPAC and Pa01C lines are the mean of nine independent experiments; data for Pa14C are the mean of eight independent experiments, and data for PANC-1 are the mean of three independent experiments. P values are from two-sided, unpaired t -test, comparing treatment conditions to DMSO, which was normalized to 100 for each measurement; error bars denote s.e.m.
    Gene Exp Myc Hs00153408 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp myc hs00153408 m1/product/Thermo Fisher
    Average 99 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    gene exp myc hs00153408 m1 - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of iSS-predicted effects of 25OHD or 1,25(OH) 2 D on monocyte expression of CAMP with observed in vitro responses of monocytes to treatment with these metabolites. Adherent human monocytes were incubated for 6 hrs in media containing 5% serum with doses of (A) 25OHD (1–300 nM) and (B) 1,25(OH) 2 D (0.1–6 nM). The experimental data is indicated by blue dots and error bars (± SD). Black lines indicate data predicted by the iSS mathematical model assuming basal levels of VDR and CYP27B1 (i.e. no activation). For the purpose of this modeling, DBP was represented by the GC1F/1F allelic combination.

    Journal: PLoS ONE

    Article Title: Vitamin D Binding Protein and Monocyte Response to 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D: Analysis by Mathematical Modeling

    doi: 10.1371/journal.pone.0030773

    Figure Lengend Snippet: Comparison of iSS-predicted effects of 25OHD or 1,25(OH) 2 D on monocyte expression of CAMP with observed in vitro responses of monocytes to treatment with these metabolites. Adherent human monocytes were incubated for 6 hrs in media containing 5% serum with doses of (A) 25OHD (1–300 nM) and (B) 1,25(OH) 2 D (0.1–6 nM). The experimental data is indicated by blue dots and error bars (± SD). Black lines indicate data predicted by the iSS mathematical model assuming basal levels of VDR and CYP27B1 (i.e. no activation). For the purpose of this modeling, DBP was represented by the GC1F/1F allelic combination.

    Article Snippet: Specifically, we utilized FAM-labeled TaqMan Gene Expression Assay probe/primer Hs00189038_m1 (CAMP) in conjunction with VIC/MGB Probe/Primer Eukaryotic 18S rRNA Endogenous Control (part number 4319413E) as the internal calibrator.

    Techniques: Expressing, In Vitro, Incubation, Activation Assay

    Schematic framework of parameters used to produce extracellular steady state (eSS) and intracellular (iSS) mathematical models for vitamin D metabolism and function. Free 25OHD and 1,25(OH) 2 D interacting with extra-cellular vitamin D binding protein (DBP) or albumin indicated in black text and arrows (eSS model). Intra-cellular interactions involving the vitamin D-activating enzyme (CYP27B1), the vitamin D receptor (VDR) and transcriptional induction of the antibacterial protein CAMP via interaction between VDR and the CAMP gene promoter (CAMP-DNA) indicated by grey text and arrows (iSS model).

    Journal: PLoS ONE

    Article Title: Vitamin D Binding Protein and Monocyte Response to 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D: Analysis by Mathematical Modeling

    doi: 10.1371/journal.pone.0030773

    Figure Lengend Snippet: Schematic framework of parameters used to produce extracellular steady state (eSS) and intracellular (iSS) mathematical models for vitamin D metabolism and function. Free 25OHD and 1,25(OH) 2 D interacting with extra-cellular vitamin D binding protein (DBP) or albumin indicated in black text and arrows (eSS model). Intra-cellular interactions involving the vitamin D-activating enzyme (CYP27B1), the vitamin D receptor (VDR) and transcriptional induction of the antibacterial protein CAMP via interaction between VDR and the CAMP gene promoter (CAMP-DNA) indicated by grey text and arrows (iSS model).

    Article Snippet: Specifically, we utilized FAM-labeled TaqMan Gene Expression Assay probe/primer Hs00189038_m1 (CAMP) in conjunction with VIC/MGB Probe/Primer Eukaryotic 18S rRNA Endogenous Control (part number 4319413E) as the internal calibrator.

    Techniques: Binding Assay

    Radio-pathologic and genetic findings in proband with GPD1 mutation. ( a ) Liver biopsy: the liver parenchyma shows macro- and microvesicular fatty change on Hematoxylin and Eosin staining (H E), magnification × 10. ( b and c ) Ultrasound

    Journal: European Journal of Human Genetics

    Article Title: A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia

    doi: 10.1038/ejhg.2014.8

    Figure Lengend Snippet: Radio-pathologic and genetic findings in proband with GPD1 mutation. ( a ) Liver biopsy: the liver parenchyma shows macro- and microvesicular fatty change on Hematoxylin and Eosin staining (H E), magnification × 10. ( b and c ) Ultrasound

    Article Snippet: The probes for both GPD1 (Hs00911535_cn, FAM labeled) and copy number reference gene (4403326, VIC-labeled RNase P ( RPPH1 )) are commercially available.

    Techniques: Mutagenesis, Staining

    Western blot analysis on liver biopsy sample from the proband and age-matched control. GPD1 protein was absent in the proband although similar amounts of GPD2 and CPT1A were present in the proband and control.

    Journal: European Journal of Human Genetics

    Article Title: A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia

    doi: 10.1038/ejhg.2014.8

    Figure Lengend Snippet: Western blot analysis on liver biopsy sample from the proband and age-matched control. GPD1 protein was absent in the proband although similar amounts of GPD2 and CPT1A were present in the proband and control.

    Article Snippet: The probes for both GPD1 (Hs00911535_cn, FAM labeled) and copy number reference gene (4403326, VIC-labeled RNase P ( RPPH1 )) are commercially available.

    Techniques: Western Blot

    ERK inhibition and KRAS silencing impairs mitochondrial function in PDAC cells. a , Cells were treated with vehicle (DMSO) or SCH772984 (ERKi, 1 μM) for 24 h. Shown is quantification of mean fluorescence of MitoTracker green staining, normalized to DMSO for each cell line across three independent experiments. P values are from two-sided, unpaired t -test; error bars denote s.e.m. b , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, anti-TOMM20; blue, DAPI. Scale bar, 20 μm. c , Quantitation of mitochondrial morphologies observed in cells shown in b . Some 50 cells per condition per repetition were blindly scored and data are averages of four independent experiments; error bars denote s.e.m. d , Pa14C cells were treated with SCH772984 (ERKi, 1 µM) for 1 h. Immunoblot of phosphorylated DRP1 (S616) (pDRP1), total DRP1 and β-actin is representative of three independent experiments. e , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, Anti-TOMM20; blue, DAPI; scale bar, 20 μm. f , Quantitation of mitochondrial morphologies observed in cells shown in e . Some 50 cells per condition per repetition were blindly scored; data are means of four independent experiments; error bars denote s.e.m. g , PDAC cells were treated with SCH772984 (ERKi, 1 μM) for 24 h. Immunoblot of pDRP1, total DRP1, MYC, phosphorylated RSK (pRSK) and β-actin is representative of three independent experiments. h , Cell lines derived from the iKRAS PDAC mouse model were deprived of doxycycline (Dox) to turn off Kras G12D for 24 h. Representative transmission electron microscopy (TEM) micrographs displaying mitochondrial morphologies in the presence and absence of doxycycline; images are representative of six independent experiments; scale bar, 500 nm. i , Cells were treated as described in a . Quantification of relative mitochondrial potential via labeling with the JC-1 mitochondrial dye. Data are the mean of three independent experiments; P values are from two-sided, unpaired t -test; error bars denote s.d. of median ratios of JC-1 aggregate fluorescence to JC-1 monomer fluorescence. j , Representative histograms from Pa01C and Pa14C cells quantified in i ; as a control, the uncoupler CCCP was added to acquired DMSO samples to establish minimum potential. k , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 1.5 h; shown is mean OCR of three independent experiments; error bars denote s.e.m. of mean OCR across replicates. l , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 24 h. Data for HPAC and Pa01C lines are the mean of nine independent experiments; data for Pa14C are the mean of eight independent experiments, and data for PANC-1 are the mean of three independent experiments. P values are from two-sided, unpaired t -test, comparing treatment conditions to DMSO, which was normalized to 100 for each measurement; error bars denote s.e.m.

    Journal: Nature medicine

    Article Title: Combination of ERK and autophagy inhibition as a treatment approach for pancreatic cancer

    doi: 10.1038/s41591-019-0368-8

    Figure Lengend Snippet: ERK inhibition and KRAS silencing impairs mitochondrial function in PDAC cells. a , Cells were treated with vehicle (DMSO) or SCH772984 (ERKi, 1 μM) for 24 h. Shown is quantification of mean fluorescence of MitoTracker green staining, normalized to DMSO for each cell line across three independent experiments. P values are from two-sided, unpaired t -test; error bars denote s.e.m. b , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, anti-TOMM20; blue, DAPI. Scale bar, 20 μm. c , Quantitation of mitochondrial morphologies observed in cells shown in b . Some 50 cells per condition per repetition were blindly scored and data are averages of four independent experiments; error bars denote s.e.m. d , Pa14C cells were treated with SCH772984 (ERKi, 1 µM) for 1 h. Immunoblot of phosphorylated DRP1 (S616) (pDRP1), total DRP1 and β-actin is representative of three independent experiments. e , Mitochondrial morphologies of PDAC cells treated with SCH772984 (ERKi, 1 µM) for 24 h. Green, Anti-TOMM20; blue, DAPI; scale bar, 20 μm. f , Quantitation of mitochondrial morphologies observed in cells shown in e . Some 50 cells per condition per repetition were blindly scored; data are means of four independent experiments; error bars denote s.e.m. g , PDAC cells were treated with SCH772984 (ERKi, 1 μM) for 24 h. Immunoblot of pDRP1, total DRP1, MYC, phosphorylated RSK (pRSK) and β-actin is representative of three independent experiments. h , Cell lines derived from the iKRAS PDAC mouse model were deprived of doxycycline (Dox) to turn off Kras G12D for 24 h. Representative transmission electron microscopy (TEM) micrographs displaying mitochondrial morphologies in the presence and absence of doxycycline; images are representative of six independent experiments; scale bar, 500 nm. i , Cells were treated as described in a . Quantification of relative mitochondrial potential via labeling with the JC-1 mitochondrial dye. Data are the mean of three independent experiments; P values are from two-sided, unpaired t -test; error bars denote s.d. of median ratios of JC-1 aggregate fluorescence to JC-1 monomer fluorescence. j , Representative histograms from Pa01C and Pa14C cells quantified in i ; as a control, the uncoupler CCCP was added to acquired DMSO samples to establish minimum potential. k , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 1.5 h; shown is mean OCR of three independent experiments; error bars denote s.e.m. of mean OCR across replicates. l , OCR response of PDAC cells treated with DMSO or SCH772984 (ERKi, 1 μM) for 24 h. Data for HPAC and Pa01C lines are the mean of nine independent experiments; data for Pa14C are the mean of eight independent experiments, and data for PANC-1 are the mean of three independent experiments. P values are from two-sided, unpaired t -test, comparing treatment conditions to DMSO, which was normalized to 100 for each measurement; error bars denote s.e.m.

    Article Snippet: Real-time quantitative Taqman PCR was performed on the QuantStudio 6 Flex (Thermo Fisher) with minor groove binder FAM dye-labeled probes against MYC (Hs00153408_m1, Thermo Fisher), SQSTM1 (Hs1061917_g1, Thermo Fisher), GABARAPL1 (Hs00740588_mH, Thermo Fisher), WIPI1 (Hs00924447_m1, Thermo Fisher), BECN1 (Hs01007018_m1, Thermo Fisher) and PRKAA1 (Hs01562315_m1, Thermo Fisher) and endogenous control VIC and TAMRA dye-labeled ACTB (Thermo Fisher).

    Techniques: Inhibition, Fluorescence, Staining, Quantitation Assay, Derivative Assay, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Labeling