fam labeled fluorogenic probe  (Thermo Fisher)


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    Name:
    Silencer FAM labeled Negative Control No 1 siRNA
    Description:
    Ambion A fluorescently labeled negative control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy Purified annealed and ready to use the 5 nmol aliquot is supplied in a stable dried format • Simplifies the monitoring of siRNA delivery• Functionally tested in several common cell lines• HPLC purified duplexed and ready to useVisualize siRNA Uptake with Labeled siRNAsFluorescent labeled siRNAs allow for the direct observation siRNA cellular uptake distribution and localization Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions In general siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours Fluorescent labeling of siRNAs allows for rapid monitoring of siRNA delivery by fluorescence microscopy as opposed to unlabeled siRNAs whose delivery must be monitored by target gene knockdown Silencer FAM Labeled Negative Control 1 siRNAs provide an easy way to monitor siRNA uptake by chemical transfection The sequences is identical to that of the Ambion Silencer Negative Control 1 siRNA SKU AM4611 but with a fluorescein derivative FAM 5 carboxyfluorescein excitation max 494 nm emission max 520 nm on the 5 end of the sense strand making it highly fluorescent when excited by the appropriate wavelength of light Accessory Products For the monitoring of siRNA delivery by electroporation Silencer Cy 3 Labeled Negative Control 1 siRNA SKU AM4621 is recommended because of its brighter fluorescence signal visible over a broader pH range Electroporation produces a more diffuse siRNA distribution pattern resulting in a less intense fluorescence signal than with chemical transfection Silencer control siRNAs are unmodified 21 mers they are designed to be used with Silencer siRNAs BLOCK iT siRNAs and other unmodified 21mer siRNAs If you are using other siRNA products such as Ambion Silencer Select siRNAs or STEALTH RNAi siRNAs we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results
    Catalog Number:
    am4620
    Price:
    None
    Applications:
    Cell Culture|RNAi|RNAi Transfection|RNAi, Epigenetics & Non-Coding RNA Research|Synthetic siRNA Transfection|siRNA|Transfection
    Category:
    Standards Ladders Controls
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    Structured Review

    Thermo Fisher fam labeled fluorogenic probe
    Ambion A fluorescently labeled negative control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy Purified annealed and ready to use the 5 nmol aliquot is supplied in a stable dried format • Simplifies the monitoring of siRNA delivery• Functionally tested in several common cell lines• HPLC purified duplexed and ready to useVisualize siRNA Uptake with Labeled siRNAsFluorescent labeled siRNAs allow for the direct observation siRNA cellular uptake distribution and localization Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions In general siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours Fluorescent labeling of siRNAs allows for rapid monitoring of siRNA delivery by fluorescence microscopy as opposed to unlabeled siRNAs whose delivery must be monitored by target gene knockdown Silencer FAM Labeled Negative Control 1 siRNAs provide an easy way to monitor siRNA uptake by chemical transfection The sequences is identical to that of the Ambion Silencer Negative Control 1 siRNA SKU AM4611 but with a fluorescein derivative FAM 5 carboxyfluorescein excitation max 494 nm emission max 520 nm on the 5 end of the sense strand making it highly fluorescent when excited by the appropriate wavelength of light Accessory Products For the monitoring of siRNA delivery by electroporation Silencer Cy 3 Labeled Negative Control 1 siRNA SKU AM4621 is recommended because of its brighter fluorescence signal visible over a broader pH range Electroporation produces a more diffuse siRNA distribution pattern resulting in a less intense fluorescence signal than with chemical transfection Silencer control siRNAs are unmodified 21 mers they are designed to be used with Silencer siRNAs BLOCK iT siRNAs and other unmodified 21mer siRNAs If you are using other siRNA products such as Ambion Silencer Select siRNAs or STEALTH RNAi siRNAs we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results
    https://www.bioz.com/result/fam labeled fluorogenic probe/product/Thermo Fisher
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    fam labeled fluorogenic probe - by Bioz Stars, 2020-12
    96/100 stars

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    Related Articles

    Negative Control:

    Article Title: In Vitro Gene Silencing of the Fish Microsporidian Heterosporis saurida by RNA Interference
    Article Snippet: .. To optimize transfection efficiency, we used the silencer FAM-labeled negative control No. 1 siRNA (#AM 4620; Ambion), which is unrelated to H. saurida and A. japonica and does not target any gene products. .. Additionally, this control does not have any sequence homology to Mus musculus , Rattus norvegicus , and Homo sapiens , and has been screened by the manufacturer in cell cultures, and confirmed to have no significant effect on cell proliferation, viability, or morphology.

    Article Title: Controlled and sustained delivery of siRNA/NPs from hydrogels expedites bone fracture healing
    Article Snippet: .. Silencer ® FAM (Fluorescein derivative, 5’-carboxyfluorescein)-labeled Negative Control No. 1 siRNA (Cat. # AM4620, Ambion® ) was incubated with NPs to form complexes as described. ..

    Article Title: Targeted gene silencing in vivo by platelet membrane–coated metal-organic framework nanoparticles
    Article Snippet: .. Silencer 5′-carboxyfluorescein (FAM)–labeled negative control #1 siRNA (Invitrogen) was used for characterization studies, and unlabeled silencer negative control #1 siRNA (siRNANC ; Invitrogen), silencer siRNA against GFP (siRNAGFP ; Invitrogen), and siRNA against survivin (siRNASur ; Santa Cruz Biotechnology) were used in the functional studies. .. For membrane coating, human platelet membrane was derived from type O+ platelet-rich plasma (San Diego Blood Bank) by a freeze-thaw process and suspended at 2 mg/ml in water ( ).

    Article Title: In Vitro Gene Silencing of the Fish Microsporidian Heterosporis saurida by RNA Interference
    Article Snippet: .. Optimization of transfection efficiency According to the manufacturer's instructions, 1 μm, 1.5 and 2 μM of the silencer FAM-labeled negative control No. 1 siRNA (#AM 4620; Ambion) were mixed with equal volumes of Lipofectamine RNAiMAX (Invitrogen). .. The silencer FAM-labeled negative control siRNA was resuspended in Opti-MEM reduced-serum medium aliquots (Invitrogen) and incubated with Lipofectamine RNAiMAX reagent at room temperature for 30 min. EK-1 cells were seeded into 24-well plates at 1 × 105 cells per well in serum-free L-15 medium.

    Article Title: In Vitro Gene Silencing of the Fish Microsporidian Heterosporis saurida by RNA Interference
    Article Snippet: .. The silencer FAM-labeled negative control siRNA was resuspended in Opti-MEM reduced-serum medium aliquots (Invitrogen) and incubated with Lipofectamine RNAiMAX reagent at room temperature for 30 min. EK-1 cells were seeded into 24-well plates at 1 × 105 cells per well in serum-free L-15 medium. .. Cultures were allowed to incubate with the silencer FAM-labeled negative control with siRNA-Lipofectamine RNAiMAX complexes for 4 h at 26°C, media were replaced with L-15 medium containing 10% FBS, and then cells were observed daily using fluorescence microscopy.

    Article Title: Controlled and sustained delivery of siRNA/NPs from hydrogels expedites bone fracture healing
    Article Snippet: .. Silencer ® FAM-labeled Negative Control No. 1 siRNA (Cat. # AM4620, Ambion® ) was incubated with NPs to form complexes as previously described which were incorporated into PEG hydrogels at overall siRNA concentration of 1 µM in 40 µl hydrogels. ..

    Article Title: Type I Interferons and Interferon Regulatory Factors Regulate TNF-Related Apoptosis-Inducing Ligand (TRAIL) in HIV-1-Infected Macrophages
    Article Snippet: .. To evaluate transfection efficiency, control and HIV-1-infected MDM were transfected with Silencer FAM-labeled Negative Control #1 siRNA (green fluorescence tagged siRNA) (Ambion Inc.). .. At 48 hours post-transfection, cells were incubated with Hoechst 33342 (Sigma) for nuclear staining, transfected and total cells were counted.

    Article Title: PID1 increases chemotherapy-induced apoptosis in medulloblastoma and glioblastoma cells in a manner that involves NFκB
    Article Snippet: .. FAM-labeled non-silencing siRNA negative control was from Life Technologies. .. For transfection, siControl (mix of 100 nM non-silencing siRNA + 10 nM FAM-labeled non-silencing siRNA control) or siPID1 (100 nM of 1:1 mix of the two PID1 -specific siRNAs + 10 nM FAM-labeled non-silencing siRNA control) were transfected using lipofectamine2000 for 5 hrs.

    Fluorescence:

    Article Title: Type I Interferons and Interferon Regulatory Factors Regulate TNF-Related Apoptosis-Inducing Ligand (TRAIL) in HIV-1-Infected Macrophages
    Article Snippet: .. To evaluate transfection efficiency, control and HIV-1-infected MDM were transfected with Silencer FAM-labeled Negative Control #1 siRNA (green fluorescence tagged siRNA) (Ambion Inc.). .. At 48 hours post-transfection, cells were incubated with Hoechst 33342 (Sigma) for nuclear staining, transfected and total cells were counted.

    Functional Assay:

    Article Title: Targeted gene silencing in vivo by platelet membrane–coated metal-organic framework nanoparticles
    Article Snippet: .. Silencer 5′-carboxyfluorescein (FAM)–labeled negative control #1 siRNA (Invitrogen) was used for characterization studies, and unlabeled silencer negative control #1 siRNA (siRNANC ; Invitrogen), silencer siRNA against GFP (siRNAGFP ; Invitrogen), and siRNA against survivin (siRNASur ; Santa Cruz Biotechnology) were used in the functional studies. .. For membrane coating, human platelet membrane was derived from type O+ platelet-rich plasma (San Diego Blood Bank) by a freeze-thaw process and suspended at 2 mg/ml in water ( ).

    Concentration Assay:

    Article Title: Controlled and sustained delivery of siRNA/NPs from hydrogels expedites bone fracture healing
    Article Snippet: .. Silencer ® FAM-labeled Negative Control No. 1 siRNA (Cat. # AM4620, Ambion® ) was incubated with NPs to form complexes as previously described which were incorporated into PEG hydrogels at overall siRNA concentration of 1 µM in 40 µl hydrogels. ..

    Incubation:

    Article Title: Controlled and sustained delivery of siRNA/NPs from hydrogels expedites bone fracture healing
    Article Snippet: .. Silencer ® FAM (Fluorescein derivative, 5’-carboxyfluorescein)-labeled Negative Control No. 1 siRNA (Cat. # AM4620, Ambion® ) was incubated with NPs to form complexes as described. ..

    Article Title: In Vitro Gene Silencing of the Fish Microsporidian Heterosporis saurida by RNA Interference
    Article Snippet: .. The silencer FAM-labeled negative control siRNA was resuspended in Opti-MEM reduced-serum medium aliquots (Invitrogen) and incubated with Lipofectamine RNAiMAX reagent at room temperature for 30 min. EK-1 cells were seeded into 24-well plates at 1 × 105 cells per well in serum-free L-15 medium. .. Cultures were allowed to incubate with the silencer FAM-labeled negative control with siRNA-Lipofectamine RNAiMAX complexes for 4 h at 26°C, media were replaced with L-15 medium containing 10% FBS, and then cells were observed daily using fluorescence microscopy.

    Article Title: Controlled and sustained delivery of siRNA/NPs from hydrogels expedites bone fracture healing
    Article Snippet: .. Silencer ® FAM-labeled Negative Control No. 1 siRNA (Cat. # AM4620, Ambion® ) was incubated with NPs to form complexes as previously described which were incorporated into PEG hydrogels at overall siRNA concentration of 1 µM in 40 µl hydrogels. ..

    Transfection:

    Article Title: In Vitro Gene Silencing of the Fish Microsporidian Heterosporis saurida by RNA Interference
    Article Snippet: .. To optimize transfection efficiency, we used the silencer FAM-labeled negative control No. 1 siRNA (#AM 4620; Ambion), which is unrelated to H. saurida and A. japonica and does not target any gene products. .. Additionally, this control does not have any sequence homology to Mus musculus , Rattus norvegicus , and Homo sapiens , and has been screened by the manufacturer in cell cultures, and confirmed to have no significant effect on cell proliferation, viability, or morphology.

    Article Title: In Vitro Gene Silencing of the Fish Microsporidian Heterosporis saurida by RNA Interference
    Article Snippet: .. Optimization of transfection efficiency According to the manufacturer's instructions, 1 μm, 1.5 and 2 μM of the silencer FAM-labeled negative control No. 1 siRNA (#AM 4620; Ambion) were mixed with equal volumes of Lipofectamine RNAiMAX (Invitrogen). .. The silencer FAM-labeled negative control siRNA was resuspended in Opti-MEM reduced-serum medium aliquots (Invitrogen) and incubated with Lipofectamine RNAiMAX reagent at room temperature for 30 min. EK-1 cells were seeded into 24-well plates at 1 × 105 cells per well in serum-free L-15 medium.

    Article Title: Type I Interferons and Interferon Regulatory Factors Regulate TNF-Related Apoptosis-Inducing Ligand (TRAIL) in HIV-1-Infected Macrophages
    Article Snippet: .. To evaluate transfection efficiency, control and HIV-1-infected MDM were transfected with Silencer FAM-labeled Negative Control #1 siRNA (green fluorescence tagged siRNA) (Ambion Inc.). .. At 48 hours post-transfection, cells were incubated with Hoechst 33342 (Sigma) for nuclear staining, transfected and total cells were counted.

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  • 99
    Thermo Fisher dual labeled internal fluorogenic fam tamra probe sets
    Dual Labeled Internal Fluorogenic Fam Tamra Probe Sets, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual labeled internal fluorogenic fam tamra probe sets/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    dual labeled internal fluorogenic fam tamra probe sets - by Bioz Stars, 2020-12
    99/100 stars
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