fam dye labeled taqman probe  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fam dye labeled taqman probe
    Fam Dye Labeled Taqman Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fam dye labeled taqman probe/product/Thermo Fisher
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    fam dye labeled taqman probe - by Bioz Stars, 2020-08
    85/100 stars

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    Related Articles

    Amplification:

    Article Title: IL-10 is pathogenic during the development of coxsackievirus B4-induced chronic pancreatitis
    Article Snippet: .. A region of the VP1 sequence was amplified with two unlabeled PCR primers (5’-TGAGCAAATCCCAGCTCTGA, 5’-TGGATCCACCTGGGAAGTATG) and a FAM-dye-labeled TaqMan probe (5’-AGCTGTGGAGACTGG) in an Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using the Absolute Quantification software. .. A standard curve (threshold cycle number (Ct) vs viral copy number), generated from the serially diluted viral cDNAs, was used to determine the viral copy number in the pancreatic test samples.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress
    Article Snippet: .. RT-PCR was performed using the Thunderbird Probe qPCR Mix (Toyobo Life Science, Osaka, Japan) and Gene Expression Assay containing primers and an FAM dye-labeled TaqMan probe for detecting human NRF2 (HS00965961-g1; Applied Biosystems, USA) and eukaryotic 18S rRNA (Hs_99999901_s1; Applied Biosystems) that is available for human 18S rRNA [ ]. .. PCR cycles consisted of a predenaturation step at 95°C for 2 min followed by 40 cycles of denaturing steps at 95°C for 15 s and annealing and extending steps at 60°C for 60 s. The relative expressions of the target genes were determined using the 2−ΔΔCt method.

    Real-time Polymerase Chain Reaction:

    Article Title: Downregulation of Mir-31, Mir-155, and Mir-564 in Chronic Myeloid Leukemia Cells
    Article Snippet: .. Real-time PCR was then performed using the SDS 7000 machine (Applied Biosystems, USA) in a 20 µl reaction containing 10 ng RNA, 10 µl TaqMan master mix (Ambion, USA), 1 µl of target gene or GUSB RNA control primers and a FAM dye-labeled TaqMan probe (Ambion, USA). .. The ΔΔCt method was used to calculate relative expression levels.

    Article Title: NAD(P)H Quinone Oxidoreductase Protects TAp63? from Proteasomal Degradation and Regulates TAp63?-Dependent Growth Arrest
    Article Snippet: .. Real-time PCR was then performed using the SDS 7000 machine (Applied Biosystems) in a 20µl reaction containing 40ng RNA, 10µl TaqMan master mix (Ambion), 1µl of target gene or 18S rRNA control primers and a FAM dye-labeled TaqMan probe (Ambion). .. The ΔΔCt method was used to calculate relative expression levels.

    Article Title: IL-10 is pathogenic during the development of coxsackievirus B4-induced chronic pancreatitis
    Article Snippet: .. A region of the VP1 sequence was amplified with two unlabeled PCR primers (5’-TGAGCAAATCCCAGCTCTGA, 5’-TGGATCCACCTGGGAAGTATG) and a FAM-dye-labeled TaqMan probe (5’-AGCTGTGGAGACTGG) in an Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using the Absolute Quantification software. .. A standard curve (threshold cycle number (Ct) vs viral copy number), generated from the serially diluted viral cDNAs, was used to determine the viral copy number in the pancreatic test samples.

    Article Title: Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress
    Article Snippet: .. RT-PCR was performed using the Thunderbird Probe qPCR Mix (Toyobo Life Science, Osaka, Japan) and Gene Expression Assay containing primers and an FAM dye-labeled TaqMan probe for detecting human NRF2 (HS00965961-g1; Applied Biosystems, USA) and eukaryotic 18S rRNA (Hs_99999901_s1; Applied Biosystems) that is available for human 18S rRNA [ ]. .. PCR cycles consisted of a predenaturation step at 95°C for 2 min followed by 40 cycles of denaturing steps at 95°C for 15 s and annealing and extending steps at 60°C for 60 s. The relative expressions of the target genes were determined using the 2−ΔΔCt method.

    Sequencing:

    Article Title: IL-10 is pathogenic during the development of coxsackievirus B4-induced chronic pancreatitis
    Article Snippet: .. A region of the VP1 sequence was amplified with two unlabeled PCR primers (5’-TGAGCAAATCCCAGCTCTGA, 5’-TGGATCCACCTGGGAAGTATG) and a FAM-dye-labeled TaqMan probe (5’-AGCTGTGGAGACTGG) in an Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using the Absolute Quantification software. .. A standard curve (threshold cycle number (Ct) vs viral copy number), generated from the serially diluted viral cDNAs, was used to determine the viral copy number in the pancreatic test samples.

    Expressing:

    Article Title: Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress
    Article Snippet: .. RT-PCR was performed using the Thunderbird Probe qPCR Mix (Toyobo Life Science, Osaka, Japan) and Gene Expression Assay containing primers and an FAM dye-labeled TaqMan probe for detecting human NRF2 (HS00965961-g1; Applied Biosystems, USA) and eukaryotic 18S rRNA (Hs_99999901_s1; Applied Biosystems) that is available for human 18S rRNA [ ]. .. PCR cycles consisted of a predenaturation step at 95°C for 2 min followed by 40 cycles of denaturing steps at 95°C for 15 s and annealing and extending steps at 60°C for 60 s. The relative expressions of the target genes were determined using the 2−ΔΔCt method.

    Polymerase Chain Reaction:

    Article Title: IL-10 is pathogenic during the development of coxsackievirus B4-induced chronic pancreatitis
    Article Snippet: .. A region of the VP1 sequence was amplified with two unlabeled PCR primers (5’-TGAGCAAATCCCAGCTCTGA, 5’-TGGATCCACCTGGGAAGTATG) and a FAM-dye-labeled TaqMan probe (5’-AGCTGTGGAGACTGG) in an Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using the Absolute Quantification software. .. A standard curve (threshold cycle number (Ct) vs viral copy number), generated from the serially diluted viral cDNAs, was used to determine the viral copy number in the pancreatic test samples.

    Software:

    Article Title: IL-10 is pathogenic during the development of coxsackievirus B4-induced chronic pancreatitis
    Article Snippet: .. A region of the VP1 sequence was amplified with two unlabeled PCR primers (5’-TGAGCAAATCCCAGCTCTGA, 5’-TGGATCCACCTGGGAAGTATG) and a FAM-dye-labeled TaqMan probe (5’-AGCTGTGGAGACTGG) in an Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using the Absolute Quantification software. .. A standard curve (threshold cycle number (Ct) vs viral copy number), generated from the serially diluted viral cDNAs, was used to determine the viral copy number in the pancreatic test samples.

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    Thermo Fisher gene exp esr1 hs00174860 m1
    Cells were transiently transfected for 24 h with 3x ERE-luc and phRL-TK Renilla internal control plasmid together with either pDsRed-express-C1 (empty vector control), <t>pDsRed-wt-ESR1</t> or pDsRed-Δ7-ESR1. Following transfection, cells were incubated for 24 h in the absence or presence of the indicated concentrations of 17β-estradiol ( A ) or 50 pM of 17β-estradiol ( B ). Values are mean +SEM averaged from n = 6 replicate cultures and presented as luciferase activity relative to the empty vector construct of the vehicle-treated control condition (*** P
    Gene Exp Esr1 Hs00174860 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp esr1 hs00174860 m1/product/Thermo Fisher
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    gene exp esr1 hs00174860 m1 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher fam dye labeled taqman mgb probe
    Cells were transiently transfected for 24 h with 3x ERE-luc and phRL-TK Renilla internal control plasmid together with either pDsRed-express-C1 (empty vector control), <t>pDsRed-wt-ESR1</t> or pDsRed-Δ7-ESR1. Following transfection, cells were incubated for 24 h in the absence or presence of the indicated concentrations of 17β-estradiol ( A ) or 50 pM of 17β-estradiol ( B ). Values are mean +SEM averaged from n = 6 replicate cultures and presented as luciferase activity relative to the empty vector construct of the vehicle-treated control condition (*** P
    Fam Dye Labeled Taqman Mgb Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fam dye labeled taqman mgb probe/product/Thermo Fisher
    Average 97 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    fam dye labeled taqman mgb probe - by Bioz Stars, 2020-08
    97/100 stars
      Buy from Supplier

    92
    Thermo Fisher gene exp klk3 hs03063374 m1
    Cells were transiently transfected for 24 h with 3x ERE-luc and phRL-TK Renilla internal control plasmid together with either pDsRed-express-C1 (empty vector control), <t>pDsRed-wt-ESR1</t> or pDsRed-Δ7-ESR1. Following transfection, cells were incubated for 24 h in the absence or presence of the indicated concentrations of 17β-estradiol ( A ) or 50 pM of 17β-estradiol ( B ). Values are mean +SEM averaged from n = 6 replicate cultures and presented as luciferase activity relative to the empty vector construct of the vehicle-treated control condition (*** P
    Gene Exp Klk3 Hs03063374 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp klk3 hs03063374 m1/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp klk3 hs03063374 m1 - by Bioz Stars, 2020-08
    92/100 stars
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    Cells were transiently transfected for 24 h with 3x ERE-luc and phRL-TK Renilla internal control plasmid together with either pDsRed-express-C1 (empty vector control), pDsRed-wt-ESR1 or pDsRed-Δ7-ESR1. Following transfection, cells were incubated for 24 h in the absence or presence of the indicated concentrations of 17β-estradiol ( A ) or 50 pM of 17β-estradiol ( B ). Values are mean +SEM averaged from n = 6 replicate cultures and presented as luciferase activity relative to the empty vector construct of the vehicle-treated control condition (*** P

    Journal: Human Molecular Genetics

    Article Title: Variants in the estrogen receptor alpha gene and its mRNA contribute to risk for schizophrenia

    doi: 10.1093/hmg/ddn130

    Figure Lengend Snippet: Cells were transiently transfected for 24 h with 3x ERE-luc and phRL-TK Renilla internal control plasmid together with either pDsRed-express-C1 (empty vector control), pDsRed-wt-ESR1 or pDsRed-Δ7-ESR1. Following transfection, cells were incubated for 24 h in the absence or presence of the indicated concentrations of 17β-estradiol ( A ) or 50 pM of 17β-estradiol ( B ). Values are mean +SEM averaged from n = 6 replicate cultures and presented as luciferase activity relative to the empty vector construct of the vehicle-treated control condition (*** P

    Article Snippet: Each qPCR reaction contained 3 µl cDNA template (corresponding to 90 ng cDNA), 5 µl Master Mix (Eurogentec, California), 0.5 µl 20X assay-on demand mix containing forward and reverse primers and FAM™ dye-labeled TaqMan® MGB probe (Hs00174860_m1) and 1.5 µl H2 O.

    Techniques: Transfection, Plasmid Preparation, Incubation, Luciferase, Activity Assay, Construct

    ( A ) The PCR products produced after nested PCR for ESR1 from cDNA derived from the DLPFC of patients with schizophrenia. Note that the samples are migrating on a slight downward diagonal. Lane 1 contains the 100 bp DNA ladder (L). The bright band in the first three sample lanes (lanes 2–4) and the last 2 lanes (15 and 16) corresponds to the expected 1380 bp amplicon (horizontal arrow). This ∼1.4 band would be predicted if all 8 coding exons were present in the ESR1 transcript (wild-type). Many bands with smaller than the predicted sizes are found in the range of 1 to 1.3 kb and can occur in different individuals (for example, compare lanes 4 to lane 5) or within the same individual (lanes 7, 8, 11, 13 and 14). The exact identities of these bands could not be definitively identified at this stage (see Materials and Methods). Note that in lanes 5, 6, 7 and 10 no wild-type bands are detected. In the 12th lane, a larger than predicted amplicon was observed (vertical arrow, 1493 bp). In panel B, the 1493 bp ESR1 transcript was confirmed to be about 100 bp longer by a separate PCR directly targeting ESR1 exons 4 and 5 in normal individuals (N) and in the patient with schizophrenia with this genomic insertion (S).

    Journal: Human Molecular Genetics

    Article Title: Variants in the estrogen receptor alpha gene and its mRNA contribute to risk for schizophrenia

    doi: 10.1093/hmg/ddn130

    Figure Lengend Snippet: ( A ) The PCR products produced after nested PCR for ESR1 from cDNA derived from the DLPFC of patients with schizophrenia. Note that the samples are migrating on a slight downward diagonal. Lane 1 contains the 100 bp DNA ladder (L). The bright band in the first three sample lanes (lanes 2–4) and the last 2 lanes (15 and 16) corresponds to the expected 1380 bp amplicon (horizontal arrow). This ∼1.4 band would be predicted if all 8 coding exons were present in the ESR1 transcript (wild-type). Many bands with smaller than the predicted sizes are found in the range of 1 to 1.3 kb and can occur in different individuals (for example, compare lanes 4 to lane 5) or within the same individual (lanes 7, 8, 11, 13 and 14). The exact identities of these bands could not be definitively identified at this stage (see Materials and Methods). Note that in lanes 5, 6, 7 and 10 no wild-type bands are detected. In the 12th lane, a larger than predicted amplicon was observed (vertical arrow, 1493 bp). In panel B, the 1493 bp ESR1 transcript was confirmed to be about 100 bp longer by a separate PCR directly targeting ESR1 exons 4 and 5 in normal individuals (N) and in the patient with schizophrenia with this genomic insertion (S).

    Article Snippet: Each qPCR reaction contained 3 µl cDNA template (corresponding to 90 ng cDNA), 5 µl Master Mix (Eurogentec, California), 0.5 µl 20X assay-on demand mix containing forward and reverse primers and FAM™ dye-labeled TaqMan® MGB probe (Hs00174860_m1) and 1.5 µl H2 O.

    Techniques: Polymerase Chain Reaction, Produced, Nested PCR, Derivative Assay, Amplification

    A schematic figure shows the general location of ESR1 common SNPs (arrows) used in association analyses. Gray horizontal bars indicate the regions of the ESR1 gene that were re-sequenced. The eight coding exons and the insertion are indicated by thin black vertical lines. ESR1 introns are shown as open horizontal bars (note thy can be grey or white). Numbers at the bottom correspond to exons and letters represent the different alternative start sites of transcription (indicated on gene with flags). Lines connecting exons C and B to exon 1 represent some of the alternative splicing. Other alternative 5′-UTR exons upstream of exon D are not shown.

    Journal: Human Molecular Genetics

    Article Title: Variants in the estrogen receptor alpha gene and its mRNA contribute to risk for schizophrenia

    doi: 10.1093/hmg/ddn130

    Figure Lengend Snippet: A schematic figure shows the general location of ESR1 common SNPs (arrows) used in association analyses. Gray horizontal bars indicate the regions of the ESR1 gene that were re-sequenced. The eight coding exons and the insertion are indicated by thin black vertical lines. ESR1 introns are shown as open horizontal bars (note thy can be grey or white). Numbers at the bottom correspond to exons and letters represent the different alternative start sites of transcription (indicated on gene with flags). Lines connecting exons C and B to exon 1 represent some of the alternative splicing. Other alternative 5′-UTR exons upstream of exon D are not shown.

    Article Snippet: Each qPCR reaction contained 3 µl cDNA template (corresponding to 90 ng cDNA), 5 µl Master Mix (Eurogentec, California), 0.5 µl 20X assay-on demand mix containing forward and reverse primers and FAM™ dye-labeled TaqMan® MGB probe (Hs00174860_m1) and 1.5 µl H2 O.

    Techniques:

    Schematic representations of the variation in transcription factor binding sites (TFBS) for the two promoter haplotypes of ESR1 are diagrammed. The top represents the common haplotype for C4, C5, C10 and C11 (1, 2, 1, 1, 1) that is not associated with the risk for schizophrenia. The bottom schematic shows the haplotype associated with risk (2, 1, 2, 2, 2). Ovals represent transcription factor binding to either the sense (upper) or antisense (lower) ESR1 DNA strand. Note the loss of a predicted biding of a transcription factor sites at C4, C5 and C11 and a gain of transcription factor binding site at C10 and Indel-C1 in the risk haplotype. Black oval, SOX5; grey ovals, MYT1; checkered oval, NGF1C; dotted oval, AP4; hatched oval, RP580 or ZNF238; white oval, myb.

    Journal: Human Molecular Genetics

    Article Title: Variants in the estrogen receptor alpha gene and its mRNA contribute to risk for schizophrenia

    doi: 10.1093/hmg/ddn130

    Figure Lengend Snippet: Schematic representations of the variation in transcription factor binding sites (TFBS) for the two promoter haplotypes of ESR1 are diagrammed. The top represents the common haplotype for C4, C5, C10 and C11 (1, 2, 1, 1, 1) that is not associated with the risk for schizophrenia. The bottom schematic shows the haplotype associated with risk (2, 1, 2, 2, 2). Ovals represent transcription factor binding to either the sense (upper) or antisense (lower) ESR1 DNA strand. Note the loss of a predicted biding of a transcription factor sites at C4, C5 and C11 and a gain of transcription factor binding site at C10 and Indel-C1 in the risk haplotype. Black oval, SOX5; grey ovals, MYT1; checkered oval, NGF1C; dotted oval, AP4; hatched oval, RP580 or ZNF238; white oval, myb.

    Article Snippet: Each qPCR reaction contained 3 µl cDNA template (corresponding to 90 ng cDNA), 5 µl Master Mix (Eurogentec, California), 0.5 µl 20X assay-on demand mix containing forward and reverse primers and FAM™ dye-labeled TaqMan® MGB probe (Hs00174860_m1) and 1.5 µl H2 O.

    Techniques: Binding Assay

    Scatter plot showing the average quantity of ESR1 mRNA levels normalized to the geometric mean of four housekeeper (HSKPs) mRNAs on the Y -axis as determined by real time qPCR. The values of ESR for patients with schizophrenia are plotted according to the PvuII (C11) genotype with C being the risk allele. Those patients homozygous for the risk allele (CC), have significantly lower expression compared to the other genotype groups (T/C and T/T). * P > 0.05.

    Journal: Human Molecular Genetics

    Article Title: Variants in the estrogen receptor alpha gene and its mRNA contribute to risk for schizophrenia

    doi: 10.1093/hmg/ddn130

    Figure Lengend Snippet: Scatter plot showing the average quantity of ESR1 mRNA levels normalized to the geometric mean of four housekeeper (HSKPs) mRNAs on the Y -axis as determined by real time qPCR. The values of ESR for patients with schizophrenia are plotted according to the PvuII (C11) genotype with C being the risk allele. Those patients homozygous for the risk allele (CC), have significantly lower expression compared to the other genotype groups (T/C and T/T). * P > 0.05.

    Article Snippet: Each qPCR reaction contained 3 µl cDNA template (corresponding to 90 ng cDNA), 5 µl Master Mix (Eurogentec, California), 0.5 µl 20X assay-on demand mix containing forward and reverse primers and FAM™ dye-labeled TaqMan® MGB probe (Hs00174860_m1) and 1.5 µl H2 O.

    Techniques: Real-time Polymerase Chain Reaction, Electron Paramagnetic Resonance, Expressing

    Schematic view of the ESR1 transcripts detected from cDNA from the DLPFC of adult humans. Nineteen different exon-deleted ESR1 mRNAs are depicted with the boxes representing the 8 known coding exons according to Genbank entry NM_000125. *the partial exon deletion, N the variant only detected in normal controls, P the variant only detected in psychiatric patients. WT = wild-type ESR1.

    Journal: Human Molecular Genetics

    Article Title: Variants in the estrogen receptor alpha gene and its mRNA contribute to risk for schizophrenia

    doi: 10.1093/hmg/ddn130

    Figure Lengend Snippet: Schematic view of the ESR1 transcripts detected from cDNA from the DLPFC of adult humans. Nineteen different exon-deleted ESR1 mRNAs are depicted with the boxes representing the 8 known coding exons according to Genbank entry NM_000125. *the partial exon deletion, N the variant only detected in normal controls, P the variant only detected in psychiatric patients. WT = wild-type ESR1.

    Article Snippet: Each qPCR reaction contained 3 µl cDNA template (corresponding to 90 ng cDNA), 5 µl Master Mix (Eurogentec, California), 0.5 µl 20X assay-on demand mix containing forward and reverse primers and FAM™ dye-labeled TaqMan® MGB probe (Hs00174860_m1) and 1.5 µl H2 O.

    Techniques: Variant Assay