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Becton Dickinson falcon 6 well plates
Falcon 6 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/falcon 6 well plates/product/Becton Dickinson
Average 85 stars, based on 4 article reviews
Price from $9.99 to $1999.99
falcon 6 well plates - by Bioz Stars, 2020-09
85/100 stars

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Article Title: Metabolism of short-chain ceramide by human cancer cells-implications for therapeutic approaches
Article Snippet: .. Cells treated in Falcon™ 6-well plates (BD Biosciences, San Jose, CA) were prepared and stained using the FITC Annexin V Apoptosis Detection Kit (BD Biosciences, San Jose, CA) according to the manufacture’s instructions. .. Briefly, cells were washed twice in PBS (Mediatech Inc, Manassas, VA) and approximately 105 cells were stained with FITC Annexin V and propidium iodide in 1X binding buffer for 15 min at room temperature in the dark.

other:

Article Title: The effect of Lipoxin A4 on the interaction between macrophage and osteoblast: possible role in the treatment of aseptic loosening
Article Snippet: Macrophages were seeded in Falcon 6-well plates (BD Bioscience, USA) at a density of 2 × 106 cells per well with 3 ml of medium.

Article Title: Chromosome-End Knockoff Strategy to Reshape Alkaloid Profiles of a Fungal Endophyte
Article Snippet: Antibiotic sensitivity tests Mycelium of each putative ΔEAS 1 knockoff strain was ground in 500 μl sterile water and aliquots were spread on PDA with and without hygromycin B (50 μg/ml) in wells of Falcon 6-well plates (Becton Dickinson and Co., Franklin Lakes, NJ).

Article Title: The antifibrotic effects of plasminogen activation occur via prostaglandin E2 synthesis in humans and mice
Article Snippet: For experimentation, cells were trypsinized, counted, and plated in Falcon 6-well plates (BD Biosciences) at 500,000–800,000 cells per well.

Article Title: Plasmin Overcomes Resistance to Prostaglandin E2 in Fibrotic Lung Fibroblasts by Reorganizing Protein Kinase A Signaling *
Article Snippet: Human fibroblasts were plated in Falcon 6-well plates (BD Biosciences) at 5 × 105 cells per well or at 2–3 × 105 cells per well for RNA silencing.

Article Title: Inhibition of protein translation as a novel mechanism for prostaglandin E2 regulation of cell functions
Article Snippet: Human fibroblasts were plated in Falcon 6-well plates (BD Biosciences, San Jose, CA, USA) at 5 × 105 cells/well, or at 2–3 × 105 cells/well for RNA silencing.

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  • 90
    Becton Dickinson 12 well polystyrene cell culture plates
    Representative SEM images of biofilms of C . ochracea wild type and TmAI2. C . ochracea ATCC27872 (A) and TmAI2 (B) were incubated anaerobically on coverglasses in <t>12-well</t> polystyrene plates for 48 h. Representative images of the biofilms are shown at the indicated magnifications. Arrowheads indicate partially vacant spaces.
    12 Well Polystyrene Cell Culture Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/12 well polystyrene cell culture plates/product/Becton Dickinson
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    12 well polystyrene cell culture plates - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    93
    Becton Dickinson 6 well tissue culture plates
    Silencing of Cx43 blocks GJIC and induces therapy resistance and clonogenicity (A) BxPc-3 cells were treated with non-specific siRNAs (siCO) or specific siRNAs directed against Cx43 (siCx43) for 3 days. The protein expression of Cx43 was analyzed by Western blot on days 1, 2 and 3 after incubation with siRNA or 3 d after incubation with siRNA at concentrations of 50, 100 or 200 pmol, as described in Fig. 3A. (B) BxPc-3 cells were treated with 50 pmol siRNA for 72 h followed by microinjection of Lucifer Yellow, and the monitoring of dye diffusion was performed as described in Fig. 1C. Pictures were extracted from the videos at distinct time points, and the gray values were calculated. The average gray value of the first neighbors (1) and second neighbors (2) and the gray value of the injected cell (0) are shown as the percentage over a period of 100 s. The data shown represent the means of 3 independent experiments. (C) The cells were treated with 50 pmol siRNA against Cx43 (siCx43) or non-specific control siRNA (siCO) for 72 h. Then, the cells were left untreated or were treated with gemcitabine for 24 h, followed by co-culture at a ratio of 1:1 for 24 h. The viability of untreated cells (CO, white bars) and of cells co-cultured with gemcitabine-treated cells (GEM-Byst, black bars) was measured using the MTT assay. (D) Three days after transfection with siCO or siCx43, 1×10 3 BxPc-3-GEM cells/well were seeded onto <t>6-well</t> plates. The cells were grown without a change of the medium for 2 weeks, followed by the evaluation of fixed and Coomassie-stained colonies containing at least 50 cells. The percentage of plating efficiency was calculated with the formula 100× number of colonies/number of seeded cells. The data shown represent the means ± SD (**p
    6 Well Tissue Culture Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 well tissue culture plates/product/Becton Dickinson
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    6 well tissue culture plates - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    91
    Becton Dickinson 12 well tissue culture plate
    Dose-dependent effects of C12 on caspase 3/7 in WT MEF MEF were grown on 12 well plates for two days, and growth medium was changed to Ringer’s solution. C12 was added at the concentrations shown for 1 hr, followed by preparation for measurement of caspase 3/7 activities in RLU. The threshold concentration for C12-triggered activation of caspase was between 1 and 10 μM. Avg +/− SD, n = 3 experiments. * p
    12 Well Tissue Culture Plate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/12 well tissue culture plate/product/Becton Dickinson
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    12 well tissue culture plate - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    85
    Becton Dickinson 12 well assay plates
    Ax21-derived peptides trigger enhanced resistance against bacteria. ( A ) Ten-d-old seedlings were grown in <t>12-well</t> plates, elicited with 1 μM Flg22 or 1 μM, 10 μM, or 100 μM axY22, axY s 22, or axY22A for 24 h, and then infected
    12 Well Assay Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/12 well assay plates/product/Becton Dickinson
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    12 well assay plates - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Representative SEM images of biofilms of C . ochracea wild type and TmAI2. C . ochracea ATCC27872 (A) and TmAI2 (B) were incubated anaerobically on coverglasses in 12-well polystyrene plates for 48 h. Representative images of the biofilms are shown at the indicated magnifications. Arrowheads indicate partially vacant spaces.

    Journal: PLoS ONE

    Article Title: Involvement of luxS in Biofilm Formation by Capnocytophaga ochracea

    doi: 10.1371/journal.pone.0147114

    Figure Lengend Snippet: Representative SEM images of biofilms of C . ochracea wild type and TmAI2. C . ochracea ATCC27872 (A) and TmAI2 (B) were incubated anaerobically on coverglasses in 12-well polystyrene plates for 48 h. Representative images of the biofilms are shown at the indicated magnifications. Arrowheads indicate partially vacant spaces.

    Article Snippet: Quantification of the mass of each biofilm Overnight cultures of C . ochracea strains were diluted with 1.0 x TS broth or 0.5 × TS broth to an OD660 of 0.02, and 2-ml aliquots of the cell suspensions were inoculated into each well of 12-well polystyrene cell culture plates (BD Falcon, Franklin Lakes, NJ, USA), and incubated for 24 h or 48 h. The mass of each biofilm was quantified by staining with crystal violet as described previously [ ].

    Techniques: Incubation

    Biofilm formation by C . ochracea wild type and TmAI2. C . ochracea ATCC27872 and TmAI2 were incubated in (A) 1.0 x TS broth for 24 h, (B) 1.0 x TS broth for 48 h, (C) 0.5 × TS broth for 24 h, or (D) 0.5 × TS broth for 48 h, in a 12-well plate under anaerobic conditions. Biofilm formation was then assayed by crystal violet staining. Data are presented as means ± SD (n = 10). *, P

    Journal: PLoS ONE

    Article Title: Involvement of luxS in Biofilm Formation by Capnocytophaga ochracea

    doi: 10.1371/journal.pone.0147114

    Figure Lengend Snippet: Biofilm formation by C . ochracea wild type and TmAI2. C . ochracea ATCC27872 and TmAI2 were incubated in (A) 1.0 x TS broth for 24 h, (B) 1.0 x TS broth for 48 h, (C) 0.5 × TS broth for 24 h, or (D) 0.5 × TS broth for 48 h, in a 12-well plate under anaerobic conditions. Biofilm formation was then assayed by crystal violet staining. Data are presented as means ± SD (n = 10). *, P

    Article Snippet: Quantification of the mass of each biofilm Overnight cultures of C . ochracea strains were diluted with 1.0 x TS broth or 0.5 × TS broth to an OD660 of 0.02, and 2-ml aliquots of the cell suspensions were inoculated into each well of 12-well polystyrene cell culture plates (BD Falcon, Franklin Lakes, NJ, USA), and incubated for 24 h or 48 h. The mass of each biofilm was quantified by staining with crystal violet as described previously [ ].

    Techniques: Incubation, Staining

    Representative CSLM images of biofilms of C . ochracea wild type and TmAI2. C . ochracea ATCC27872 (A) and TmAI2 (B) were incubated in 12-well polystyrene plates containing coverglass plates for 48 h, and CSLM images of the cells that attached to the coverglass were obtained. The upper panels (i) show each x - y images, and the lower panels (ii) show the center of each x - z reconstructions. Scale bars, 50 μm.

    Journal: PLoS ONE

    Article Title: Involvement of luxS in Biofilm Formation by Capnocytophaga ochracea

    doi: 10.1371/journal.pone.0147114

    Figure Lengend Snippet: Representative CSLM images of biofilms of C . ochracea wild type and TmAI2. C . ochracea ATCC27872 (A) and TmAI2 (B) were incubated in 12-well polystyrene plates containing coverglass plates for 48 h, and CSLM images of the cells that attached to the coverglass were obtained. The upper panels (i) show each x - y images, and the lower panels (ii) show the center of each x - z reconstructions. Scale bars, 50 μm.

    Article Snippet: Quantification of the mass of each biofilm Overnight cultures of C . ochracea strains were diluted with 1.0 x TS broth or 0.5 × TS broth to an OD660 of 0.02, and 2-ml aliquots of the cell suspensions were inoculated into each well of 12-well polystyrene cell culture plates (BD Falcon, Franklin Lakes, NJ, USA), and incubated for 24 h or 48 h. The mass of each biofilm was quantified by staining with crystal violet as described previously [ ].

    Techniques: Incubation

    Effect of extrinsic AI-2 on biofilm formation by the luxS -deficient mutant and wild-type strain. (A) Assessment of biofilm formation by mutant and wild-type strains by using a two-compartment system. C . ochracea ATCC 27872 and TmAI2 were inoculated into TS broth in the indicated compartments (upper or lower well) in the wells of a 12-well polystyrene plate, and were then incubated for 48 h. Biofilm formation by the strain in the lower compartment of each well was stained with crystal violet staining. After rinsing and ethanol extraction, the mass of biofilm was quantified as OD 595 of extracted stain. Data are presented as means ± SD (n = 10). *, P

    Journal: PLoS ONE

    Article Title: Involvement of luxS in Biofilm Formation by Capnocytophaga ochracea

    doi: 10.1371/journal.pone.0147114

    Figure Lengend Snippet: Effect of extrinsic AI-2 on biofilm formation by the luxS -deficient mutant and wild-type strain. (A) Assessment of biofilm formation by mutant and wild-type strains by using a two-compartment system. C . ochracea ATCC 27872 and TmAI2 were inoculated into TS broth in the indicated compartments (upper or lower well) in the wells of a 12-well polystyrene plate, and were then incubated for 48 h. Biofilm formation by the strain in the lower compartment of each well was stained with crystal violet staining. After rinsing and ethanol extraction, the mass of biofilm was quantified as OD 595 of extracted stain. Data are presented as means ± SD (n = 10). *, P

    Article Snippet: Quantification of the mass of each biofilm Overnight cultures of C . ochracea strains were diluted with 1.0 x TS broth or 0.5 × TS broth to an OD660 of 0.02, and 2-ml aliquots of the cell suspensions were inoculated into each well of 12-well polystyrene cell culture plates (BD Falcon, Franklin Lakes, NJ, USA), and incubated for 24 h or 48 h. The mass of each biofilm was quantified by staining with crystal violet as described previously [ ].

    Techniques: Mutagenesis, Incubation, Staining

    Silencing of Cx43 blocks GJIC and induces therapy resistance and clonogenicity (A) BxPc-3 cells were treated with non-specific siRNAs (siCO) or specific siRNAs directed against Cx43 (siCx43) for 3 days. The protein expression of Cx43 was analyzed by Western blot on days 1, 2 and 3 after incubation with siRNA or 3 d after incubation with siRNA at concentrations of 50, 100 or 200 pmol, as described in Fig. 3A. (B) BxPc-3 cells were treated with 50 pmol siRNA for 72 h followed by microinjection of Lucifer Yellow, and the monitoring of dye diffusion was performed as described in Fig. 1C. Pictures were extracted from the videos at distinct time points, and the gray values were calculated. The average gray value of the first neighbors (1) and second neighbors (2) and the gray value of the injected cell (0) are shown as the percentage over a period of 100 s. The data shown represent the means of 3 independent experiments. (C) The cells were treated with 50 pmol siRNA against Cx43 (siCx43) or non-specific control siRNA (siCO) for 72 h. Then, the cells were left untreated or were treated with gemcitabine for 24 h, followed by co-culture at a ratio of 1:1 for 24 h. The viability of untreated cells (CO, white bars) and of cells co-cultured with gemcitabine-treated cells (GEM-Byst, black bars) was measured using the MTT assay. (D) Three days after transfection with siCO or siCx43, 1×10 3 BxPc-3-GEM cells/well were seeded onto 6-well plates. The cells were grown without a change of the medium for 2 weeks, followed by the evaluation of fixed and Coomassie-stained colonies containing at least 50 cells. The percentage of plating efficiency was calculated with the formula 100× number of colonies/number of seeded cells. The data shown represent the means ± SD (**p

    Journal: Oncotarget

    Article Title: Sulforaphane counteracts aggressiveness of pancreatic cancer driven by dysregulated Cx43-mediated gap junctional intercellular communication

    doi:

    Figure Lengend Snippet: Silencing of Cx43 blocks GJIC and induces therapy resistance and clonogenicity (A) BxPc-3 cells were treated with non-specific siRNAs (siCO) or specific siRNAs directed against Cx43 (siCx43) for 3 days. The protein expression of Cx43 was analyzed by Western blot on days 1, 2 and 3 after incubation with siRNA or 3 d after incubation with siRNA at concentrations of 50, 100 or 200 pmol, as described in Fig. 3A. (B) BxPc-3 cells were treated with 50 pmol siRNA for 72 h followed by microinjection of Lucifer Yellow, and the monitoring of dye diffusion was performed as described in Fig. 1C. Pictures were extracted from the videos at distinct time points, and the gray values were calculated. The average gray value of the first neighbors (1) and second neighbors (2) and the gray value of the injected cell (0) are shown as the percentage over a period of 100 s. The data shown represent the means of 3 independent experiments. (C) The cells were treated with 50 pmol siRNA against Cx43 (siCx43) or non-specific control siRNA (siCO) for 72 h. Then, the cells were left untreated or were treated with gemcitabine for 24 h, followed by co-culture at a ratio of 1:1 for 24 h. The viability of untreated cells (CO, white bars) and of cells co-cultured with gemcitabine-treated cells (GEM-Byst, black bars) was measured using the MTT assay. (D) Three days after transfection with siCO or siCx43, 1×10 3 BxPc-3-GEM cells/well were seeded onto 6-well plates. The cells were grown without a change of the medium for 2 weeks, followed by the evaluation of fixed and Coomassie-stained colonies containing at least 50 cells. The percentage of plating efficiency was calculated with the formula 100× number of colonies/number of seeded cells. The data shown represent the means ± SD (**p

    Article Snippet: Colony Forming Assay Three days after siRNA transfection, 1×103 BxPc-3-GEM cells/well were seeded into 6-well tissue culture plates (BD Falcon™, San José, CA, USA), and colony forming assays were performed as previously described [ ].

    Techniques: Expressing, Western Blot, Incubation, Diffusion-based Assay, Injection, Co-Culture Assay, Cell Culture, MTT Assay, Transfection, Staining

    Dose-dependent effects of C12 on caspase 3/7 in WT MEF MEF were grown on 12 well plates for two days, and growth medium was changed to Ringer’s solution. C12 was added at the concentrations shown for 1 hr, followed by preparation for measurement of caspase 3/7 activities in RLU. The threshold concentration for C12-triggered activation of caspase was between 1 and 10 μM. Avg +/− SD, n = 3 experiments. * p

    Journal: Cellular microbiology

    Article Title: Pseudomonas aeruginosa Homoserine Lactone Triggers Apoptosis and Bak/Bax-Independent Release of Mitochondrial Cytochrome C in Fibroblasts

    doi: 10.1111/cmi.12263

    Figure Lengend Snippet: Dose-dependent effects of C12 on caspase 3/7 in WT MEF MEF were grown on 12 well plates for two days, and growth medium was changed to Ringer’s solution. C12 was added at the concentrations shown for 1 hr, followed by preparation for measurement of caspase 3/7 activities in RLU. The threshold concentration for C12-triggered activation of caspase was between 1 and 10 μM. Avg +/− SD, n = 3 experiments. * p

    Article Snippet: The cells were passaged at 1:5-1:10 dilutions and the remaining cell suspension was seeded directly onto a 96-well, 24-well or 12-well tissue culture plate (BD Falcon, Bedford, MA) or onto coverglasses for imaging.

    Techniques: Concentration Assay, Activation Assay

    Ax21-derived peptides trigger enhanced resistance against bacteria. ( A ) Ten-d-old seedlings were grown in 12-well plates, elicited with 1 μM Flg22 or 1 μM, 10 μM, or 100 μM axY22, axY s 22, or axY22A for 24 h, and then infected

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The Arabidopsis flagellin receptor FLS2 mediates the perception of Xanthomonas Ax21 secreted peptides

    doi: 10.1073/pnas.1106366108

    Figure Lengend Snippet: Ax21-derived peptides trigger enhanced resistance against bacteria. ( A ) Ten-d-old seedlings were grown in 12-well plates, elicited with 1 μM Flg22 or 1 μM, 10 μM, or 100 μM axY22, axY s 22, or axY22A for 24 h, and then infected

    Article Snippet: For growing seedlings in liquid medium in 12-well assay plates (BD Falcon; 353043), seeds were sterilized in 20% bleach (2 min), washed three times with sterile water, and 20 to 30 seeds were dispensed into wells containing 1 mL MS 1× medium (Murashige and Skoog basal medium with vitamins from Phytotechnology Laboratories supplemented with 0.5 g/L Mes hydrate and 0.5% sucrose at pH 5.7).

    Techniques: Derivative Assay, Infection