Structured Review

Tocris fak inhibitor pf 573228
Fak Inhibitor Pf 573228, supplied by Tocris, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor pf 573228/product/Tocris
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor pf 573228 - by Bioz Stars, 2024-02
86/100 stars

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Structured Review

Tocris fak inhibitor pf 573228
Fak Inhibitor Pf 573228, supplied by Tocris, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor pf 573228/product/Tocris
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor pf 573228 - by Bioz Stars, 2024-02
86/100 stars

Images


Structured Review

Tocris fak inhibitor pf 573228
(A and B) HBMEC were pre-incubated with indicated concentrations of the specific FAK inhibitor PF573228 and infected for 4 h and 8 h with the unencapsulated N. meningitidis strain MC58 siaD . Intracellular bacteria were defined by gentamicin protection assay. The graph shows mean values +/− S.D. of three independent experiments done in duplicate. ** P <0.01, relative to cells infected without inhibitor. (C) HBMEC were pre-incubated with the indicated concentrations of the FAK inhibitor <t>PF</t> <t>573228</t> for 1 h and infected with N. meningitidis MC58 siaD for 4 h. Cell lysates were resolved by SDS-PAGE and blotted with α-phospho-FAK Tyr 397 demonstrating that PF 573228 blocked FAK Tyr 397 phosphorylation in a dose-dependent manner. (D) 293T cells were transfected with indicated concentration of a commercial siRNA specific for FAK to limit FAK protein expression or transfected with unspecific control siRNA. siRNA transfected cells were infected with mutant strain MC58 siaD and internalized bacteria were measured by gentamicin protection assay at 4 h p.i. The graph represents mean values ± S.D. of three independent experiments done in duplicate. * P <0.05 and ** P <0.01, relative to cells transfected with the control siRNA.
Fak Inhibitor Pf 573228, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor pf 573228/product/Tocris
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor pf 573228 - by Bioz Stars, 2024-02
93/100 stars

Images

1) Product Images from "Cell Invasion by Neisseria meningitidis Requires a Functional Interplay between the Focal Adhesion Kinase, Src and Cortactin"

Article Title: Cell Invasion by Neisseria meningitidis Requires a Functional Interplay between the Focal Adhesion Kinase, Src and Cortactin

Journal: PLoS ONE

doi: 10.1371/journal.pone.0039613

(A and B) HBMEC were pre-incubated with indicated concentrations of the specific FAK inhibitor PF573228 and infected for 4 h and 8 h with the unencapsulated N. meningitidis strain MC58 siaD . Intracellular bacteria were defined by gentamicin protection assay. The graph shows mean values +/− S.D. of three independent experiments done in duplicate. ** P <0.01, relative to cells infected without inhibitor. (C) HBMEC were pre-incubated with the indicated concentrations of the FAK inhibitor PF 573228 for 1 h and infected with N. meningitidis MC58 siaD for 4 h. Cell lysates were resolved by SDS-PAGE and blotted with α-phospho-FAK Tyr 397 demonstrating that PF 573228 blocked FAK Tyr 397 phosphorylation in a dose-dependent manner. (D) 293T cells were transfected with indicated concentration of a commercial siRNA specific for FAK to limit FAK protein expression or transfected with unspecific control siRNA. siRNA transfected cells were infected with mutant strain MC58 siaD and internalized bacteria were measured by gentamicin protection assay at 4 h p.i. The graph represents mean values ± S.D. of three independent experiments done in duplicate. * P <0.05 and ** P <0.01, relative to cells transfected with the control siRNA.
Figure Legend Snippet: (A and B) HBMEC were pre-incubated with indicated concentrations of the specific FAK inhibitor PF573228 and infected for 4 h and 8 h with the unencapsulated N. meningitidis strain MC58 siaD . Intracellular bacteria were defined by gentamicin protection assay. The graph shows mean values +/− S.D. of three independent experiments done in duplicate. ** P <0.01, relative to cells infected without inhibitor. (C) HBMEC were pre-incubated with the indicated concentrations of the FAK inhibitor PF 573228 for 1 h and infected with N. meningitidis MC58 siaD for 4 h. Cell lysates were resolved by SDS-PAGE and blotted with α-phospho-FAK Tyr 397 demonstrating that PF 573228 blocked FAK Tyr 397 phosphorylation in a dose-dependent manner. (D) 293T cells were transfected with indicated concentration of a commercial siRNA specific for FAK to limit FAK protein expression or transfected with unspecific control siRNA. siRNA transfected cells were infected with mutant strain MC58 siaD and internalized bacteria were measured by gentamicin protection assay at 4 h p.i. The graph represents mean values ± S.D. of three independent experiments done in duplicate. * P <0.05 and ** P <0.01, relative to cells transfected with the control siRNA.

Techniques Used: Incubation, Infection, SDS Page, Transfection, Concentration Assay, Expressing, Mutagenesis


Structured Review

Tocris fak inhibitor pf573228
Rat VSMCs were treated with or without of FAK inhibitor <t>(PF573228,</t> 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.
Fak Inhibitor Pf573228, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor pf573228/product/Tocris
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor pf573228 - by Bioz Stars, 2024-02
93/100 stars

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1) Product Images from "Periostin Links Mechanical Strain to Inflammation in Abdominal Aortic Aneurysm"

Article Title: Periostin Links Mechanical Strain to Inflammation in Abdominal Aortic Aneurysm

Journal: PLoS ONE

doi: 10.1371/journal.pone.0079753

Rat VSMCs were treated with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.
Figure Legend Snippet: Rat VSMCs were treated with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.

Techniques Used: Western Blot, Zymography

Human AAA tissues were cut into small pieces and cultured with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) for 48 h (n = 5). Levels of phosphorylated FAK ( A ), ERK ( B ) and JNK ( C ) in tissue lysates were determined by western blotting. GAPDH served as an internal control. Protein levels of MCP-1 ( D ) and MMPs ( E-F ) in conditioned media were determined by ELISA and gelatin zymography, respectively. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control.
Figure Legend Snippet: Human AAA tissues were cut into small pieces and cultured with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) for 48 h (n = 5). Levels of phosphorylated FAK ( A ), ERK ( B ) and JNK ( C ) in tissue lysates were determined by western blotting. GAPDH served as an internal control. Protein levels of MCP-1 ( D ) and MMPs ( E-F ) in conditioned media were determined by ELISA and gelatin zymography, respectively. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control.

Techniques Used: Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay, Zymography


Structured Review

Tocris fak inhibitor pf573228
Rat VSMCs were treated with or without of FAK inhibitor <t>(PF573228,</t> 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.
Fak Inhibitor Pf573228, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor pf573228/product/Tocris
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor pf573228 - by Bioz Stars, 2024-02
93/100 stars

Images

1) Product Images from "Periostin Links Mechanical Strain to Inflammation in Abdominal Aortic Aneurysm"

Article Title: Periostin Links Mechanical Strain to Inflammation in Abdominal Aortic Aneurysm

Journal: PLoS ONE

doi: 10.1371/journal.pone.0079753

Rat VSMCs were treated with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.
Figure Legend Snippet: Rat VSMCs were treated with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.

Techniques Used: Western Blot, Zymography

Human AAA tissues were cut into small pieces and cultured with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) for 48 h (n = 5). Levels of phosphorylated FAK ( A ), ERK ( B ) and JNK ( C ) in tissue lysates were determined by western blotting. GAPDH served as an internal control. Protein levels of MCP-1 ( D ) and MMPs ( E-F ) in conditioned media were determined by ELISA and gelatin zymography, respectively. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control.
Figure Legend Snippet: Human AAA tissues were cut into small pieces and cultured with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) for 48 h (n = 5). Levels of phosphorylated FAK ( A ), ERK ( B ) and JNK ( C ) in tissue lysates were determined by western blotting. GAPDH served as an internal control. Protein levels of MCP-1 ( D ) and MMPs ( E-F ) in conditioned media were determined by ELISA and gelatin zymography, respectively. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control.

Techniques Used: Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay, Zymography


Structured Review

Tocris fak inhibitor pf573228 in vehicle
FAK inhibition induces CNTF expression in vitro. A ) C6 cells incubated for 4 hours with FAK inhibitor <t>PF573228</t> have increased levels of CNTF mRNA compared to vehicle-only cultures (Ctrl). B ) The inhibitor reduced FAK activity as shown in Western blots by the reduced levels of phosphorylated FAK (pFAK tyr-397). C ) CNTF protein was also increased in the same protein extracts, showing the rapid and robust nature of the disinhibition. Blots are representative of 4 independent observations. D ) C6 cell injury by mechanical dissociation robustly induced CNTF mRNA within 2 hours which was lost by 6 hours. E ) FAK inhibition did not augment the dissociation-induced CNTF mRNA up-regulation. Data are fold change compared to controls and are shown as average +/− SEM. In A, n = 6, D and E, n = 4.
Fak Inhibitor Pf573228 In Vehicle, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor pf573228 in vehicle/product/Tocris
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor pf573228 in vehicle - by Bioz Stars, 2024-02
93/100 stars

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1) Product Images from "Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression"

Article Title: Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression

Journal: Cell Communication and Signaling : CCS

doi: 10.1186/1478-811X-11-35

FAK inhibition induces CNTF expression in vitro. A ) C6 cells incubated for 4 hours with FAK inhibitor PF573228 have increased levels of CNTF mRNA compared to vehicle-only cultures (Ctrl). B ) The inhibitor reduced FAK activity as shown in Western blots by the reduced levels of phosphorylated FAK (pFAK tyr-397). C ) CNTF protein was also increased in the same protein extracts, showing the rapid and robust nature of the disinhibition. Blots are representative of 4 independent observations. D ) C6 cell injury by mechanical dissociation robustly induced CNTF mRNA within 2 hours which was lost by 6 hours. E ) FAK inhibition did not augment the dissociation-induced CNTF mRNA up-regulation. Data are fold change compared to controls and are shown as average +/− SEM. In A, n = 6, D and E, n = 4.
Figure Legend Snippet: FAK inhibition induces CNTF expression in vitro. A ) C6 cells incubated for 4 hours with FAK inhibitor PF573228 have increased levels of CNTF mRNA compared to vehicle-only cultures (Ctrl). B ) The inhibitor reduced FAK activity as shown in Western blots by the reduced levels of phosphorylated FAK (pFAK tyr-397). C ) CNTF protein was also increased in the same protein extracts, showing the rapid and robust nature of the disinhibition. Blots are representative of 4 independent observations. D ) C6 cell injury by mechanical dissociation robustly induced CNTF mRNA within 2 hours which was lost by 6 hours. E ) FAK inhibition did not augment the dissociation-induced CNTF mRNA up-regulation. Data are fold change compared to controls and are shown as average +/− SEM. In A, n = 6, D and E, n = 4.

Techniques Used: Inhibition, Expressing, In Vitro, Incubation, Activity Assay, Western Blot

FAK inhibition induces CNTF expression in the adult CNS in vivo . A ) Intracerebral injection of the FAK inhibitor PF573228 directly in the striatum of mice results in increased CNTF protein 4 hours later as shown in western blots. FAK activity was greatly decreased as shown by lack of phosphorylation. (pFAK). α-tubulin = internal control. Lanes are from individual animals. B ) Similar results were obtained after direct injection into the spinal cord. C ) Systemic intraperitoneal injections of the FAK inhibitors PF573228 or FAK14 induce CNTF mRNA in the spinal cord or SVZ relative to vehicle only injected mice (Ctrl). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are fold change compared to control (Ctrl) and are means +/− SEM from 3–4 mice per group.
Figure Legend Snippet: FAK inhibition induces CNTF expression in the adult CNS in vivo . A ) Intracerebral injection of the FAK inhibitor PF573228 directly in the striatum of mice results in increased CNTF protein 4 hours later as shown in western blots. FAK activity was greatly decreased as shown by lack of phosphorylation. (pFAK). α-tubulin = internal control. Lanes are from individual animals. B ) Similar results were obtained after direct injection into the spinal cord. C ) Systemic intraperitoneal injections of the FAK inhibitors PF573228 or FAK14 induce CNTF mRNA in the spinal cord or SVZ relative to vehicle only injected mice (Ctrl). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are fold change compared to control (Ctrl) and are means +/− SEM from 3–4 mice per group.

Techniques Used: Inhibition, Expressing, In Vivo, Injection, Western Blot, Activity Assay

Systemic FAK inhibitor treatment increases adult CNS neurogenesis. A ) mRNA for the proliferation marker Ki67 and the marker for proliferating neural C cell progenitors, EGFR, increased in the SVZ after systemic FAK inhibitor PF573228 treatment in mice. CNTF mRNA was increased in the same extracts (Figure 6C). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are mean fold change compared to vehicle treated mice +/− SEM, n = 3–4 mice. B ) In other mice, the systemic injections of FAK inhibitor PF573228 (n = 6) increased the number of DCX + neuroblasts in the SVZ. Data are expressed as a mean percentage of vehicle (n = 4) treated mice +/− SEM. C ) Representative confocal images of the dorsal SVZ showing increased numbers of nuclei (Hoechst) and neuroblasts (dcx) in an FAK inhibitor-injected mouse compared to a vehicle-injected mouse. Scale bar = 20 μm. CC = corpus callosum, LV = lateral ventricle, STR = striatum.
Figure Legend Snippet: Systemic FAK inhibitor treatment increases adult CNS neurogenesis. A ) mRNA for the proliferation marker Ki67 and the marker for proliferating neural C cell progenitors, EGFR, increased in the SVZ after systemic FAK inhibitor PF573228 treatment in mice. CNTF mRNA was increased in the same extracts (Figure 6C). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are mean fold change compared to vehicle treated mice +/− SEM, n = 3–4 mice. B ) In other mice, the systemic injections of FAK inhibitor PF573228 (n = 6) increased the number of DCX + neuroblasts in the SVZ. Data are expressed as a mean percentage of vehicle (n = 4) treated mice +/− SEM. C ) Representative confocal images of the dorsal SVZ showing increased numbers of nuclei (Hoechst) and neuroblasts (dcx) in an FAK inhibitor-injected mouse compared to a vehicle-injected mouse. Scale bar = 20 μm. CC = corpus callosum, LV = lateral ventricle, STR = striatum.

Techniques Used: Marker, Injection


Structured Review

Tocris fak inhibitor pf
Effects <t>of</t> <t>PF-228</t> on the phosphorylation of FAK, Akt and ERK in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 1 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, suspended AsPC-1 cells were plated onto plastic or LN-coated plates for 1 h in the continued presence or absence of PF-228. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.
Fak Inhibitor Pf, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor pf/product/Tocris
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor pf - by Bioz Stars, 2024-02
93/100 stars

Images

1) Product Images from "Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines"

Article Title: Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines

Journal: Molecular Cancer

doi: 10.1186/1476-4598-8-125

Effects of PF-228 on the phosphorylation of FAK, Akt and ERK in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 1 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, suspended AsPC-1 cells were plated onto plastic or LN-coated plates for 1 h in the continued presence or absence of PF-228. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.
Figure Legend Snippet: Effects of PF-228 on the phosphorylation of FAK, Akt and ERK in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 1 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, suspended AsPC-1 cells were plated onto plastic or LN-coated plates for 1 h in the continued presence or absence of PF-228. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.

Techniques Used: Western Blot, Expressing

Effects of PF-228 on Gem-induced apoptosis in Panc-1 cells . Panc-1 cells were pretreated with or without PF-228 (1 μM) for 1 h and then treated with or without 10 μM Gem for 72 h in the continued presence or absence of PF-228. The cells were subjected to apoptosis analysis by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling (B) and western blot analysis of cleaved caspase-3 protein expression (C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. Panc-1 cells without Gem treatment; #, P < 0.05, vs. Gem-treated Panc-1 cells without PF-228 pretreatment.
Figure Legend Snippet: Effects of PF-228 on Gem-induced apoptosis in Panc-1 cells . Panc-1 cells were pretreated with or without PF-228 (1 μM) for 1 h and then treated with or without 10 μM Gem for 72 h in the continued presence or absence of PF-228. The cells were subjected to apoptosis analysis by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling (B) and western blot analysis of cleaved caspase-3 protein expression (C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. Panc-1 cells without Gem treatment; #, P < 0.05, vs. Gem-treated Panc-1 cells without PF-228 pretreatment.

Techniques Used: Staining, Flow Cytometry, Labeling, Western Blot, Expressing

Effects of PF-228 on LN-mediated Gem chemoresistance in AsPC-1 cells . After pretreated with or without PF-228 (1 μM) for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN-coated 6-well plates and treated with Gem (0.1 μM) for 72 h in the continued presence or absence of PF-228. Cell apoptosis was examined by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling(B) and western blot analysis of cleaved caspase-3 protein expression(C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. AsPC-1 cells without Gem treatment; #, P < 0.05, vs. Gem-treated AsPC-1 cells without PF-228 pretreatment on LN.
Figure Legend Snippet: Effects of PF-228 on LN-mediated Gem chemoresistance in AsPC-1 cells . After pretreated with or without PF-228 (1 μM) for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN-coated 6-well plates and treated with Gem (0.1 μM) for 72 h in the continued presence or absence of PF-228. Cell apoptosis was examined by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling(B) and western blot analysis of cleaved caspase-3 protein expression(C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. AsPC-1 cells without Gem treatment; #, P < 0.05, vs. Gem-treated AsPC-1 cells without PF-228 pretreatment on LN.

Techniques Used: Staining, Flow Cytometry, Labeling, Western Blot, Expressing

Effects of PF-228 on the expression of apoptosis-associated proteins in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 24 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN for 24 h in the continued presence or absence of PF-228. Western blot analysis was used to detect the expression of Bad, p-Bad (pS136), p-Bad (pS112), Bcl-2, Bax and survivin. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.
Figure Legend Snippet: Effects of PF-228 on the expression of apoptosis-associated proteins in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 24 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN for 24 h in the continued presence or absence of PF-228. Western blot analysis was used to detect the expression of Bad, p-Bad (pS136), p-Bad (pS112), Bcl-2, Bax and survivin. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.

Techniques Used: Expressing, Western Blot


Structured Review

Tocris fak inhibitor
Fak Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor/product/Tocris
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor - by Bioz Stars, 2024-02
93/100 stars

Images


Structured Review

Tocris fak inhibitor pf 573228
β1 blockade overcomes lapatinib resistance in BT474 cells in 3D culture . ( A ) Parental and LRes cells were plated on lrECM and treated ± lapatinib. ( B ) The β1 inhibitory antibody AIIB2--or IgG control--was applied to parental and LRes cells on Day 0 of plating on lrECM and allowed to grow for 12 days. ( C ) Parental and LRes cells were plated in lrECM with appropriate inhibitors, allowed to grow for five days, stained with Ki67 antigen or TUNEL labeling, and imaged. ( D ) 3D cultures of BT474 parental and LRes cells treated with AIIB2 or IgG were allowed to propagate for five days, then harvested for protein and immunoblotting. Densitometry measurements were normalized to total levels--except β1, which was normalized to β-actin--and are representative of three independent experiments. ( E ) The FAK inhibitor <t>PF</t> <t>573228</t> was applied to parental and LRes cells on Day 0 of plating on lrECM and cultures were allowed to grow for 12 days.
Fak Inhibitor Pf 573228, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak inhibitor pf 573228/product/Tocris
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
fak inhibitor pf 573228 - by Bioz Stars, 2024-02
93/100 stars

Images

1) Product Images from "β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib"

Article Title: β1 integrin mediates an alternative survival pathway in breast cancer cells resistant to lapatinib

Journal: Breast Cancer Research : BCR

doi: 10.1186/bcr2936

β1 blockade overcomes lapatinib resistance in BT474 cells in 3D culture . ( A ) Parental and LRes cells were plated on lrECM and treated ± lapatinib. ( B ) The β1 inhibitory antibody AIIB2--or IgG control--was applied to parental and LRes cells on Day 0 of plating on lrECM and allowed to grow for 12 days. ( C ) Parental and LRes cells were plated in lrECM with appropriate inhibitors, allowed to grow for five days, stained with Ki67 antigen or TUNEL labeling, and imaged. ( D ) 3D cultures of BT474 parental and LRes cells treated with AIIB2 or IgG were allowed to propagate for five days, then harvested for protein and immunoblotting. Densitometry measurements were normalized to total levels--except β1, which was normalized to β-actin--and are representative of three independent experiments. ( E ) The FAK inhibitor PF 573228 was applied to parental and LRes cells on Day 0 of plating on lrECM and cultures were allowed to grow for 12 days.
Figure Legend Snippet: β1 blockade overcomes lapatinib resistance in BT474 cells in 3D culture . ( A ) Parental and LRes cells were plated on lrECM and treated ± lapatinib. ( B ) The β1 inhibitory antibody AIIB2--or IgG control--was applied to parental and LRes cells on Day 0 of plating on lrECM and allowed to grow for 12 days. ( C ) Parental and LRes cells were plated in lrECM with appropriate inhibitors, allowed to grow for five days, stained with Ki67 antigen or TUNEL labeling, and imaged. ( D ) 3D cultures of BT474 parental and LRes cells treated with AIIB2 or IgG were allowed to propagate for five days, then harvested for protein and immunoblotting. Densitometry measurements were normalized to total levels--except β1, which was normalized to β-actin--and are representative of three independent experiments. ( E ) The FAK inhibitor PF 573228 was applied to parental and LRes cells on Day 0 of plating on lrECM and cultures were allowed to grow for 12 days.

Techniques Used: Staining, TUNEL Assay, Labeling, Western Blot


Structured Review

Tocris pf 573228 fak inhibitor

Pf 573228 Fak Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pf 573228 fak inhibitor - by Bioz Stars, 2024-02
93/100 stars

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1) Product Images from "C-MORE: A high-content single-cell morphology recognition methodology for liquid biopsies toward personalized cardiovascular medicine"

Article Title: C-MORE: A high-content single-cell morphology recognition methodology for liquid biopsies toward personalized cardiovascular medicine

Journal: Cell Reports Medicine

doi: 10.1016/j.xcrm.2021.100436


Figure Legend Snippet:

Techniques Used: Recombinant, Electron Microscopy, Software, Imaging

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    Tocris fak inhibitor pf 573228
    Fak Inhibitor Pf 573228, supplied by Tocris, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fak inhibitor pf 573228/product/Tocris
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    93
    Tocris fak inhibitor pf573228
    Rat VSMCs were treated with or without of FAK inhibitor <t>(PF573228,</t> 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.
    Fak Inhibitor Pf573228, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fak inhibitor pf573228/product/Tocris
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fak inhibitor pf573228 - by Bioz Stars, 2024-02
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    Tocris fak inhibitor pf573228 in vehicle
    FAK inhibition induces CNTF expression in vitro. A ) C6 cells incubated for 4 hours with FAK inhibitor <t>PF573228</t> have increased levels of CNTF mRNA compared to vehicle-only cultures (Ctrl). B ) The inhibitor reduced FAK activity as shown in Western blots by the reduced levels of phosphorylated FAK (pFAK tyr-397). C ) CNTF protein was also increased in the same protein extracts, showing the rapid and robust nature of the disinhibition. Blots are representative of 4 independent observations. D ) C6 cell injury by mechanical dissociation robustly induced CNTF mRNA within 2 hours which was lost by 6 hours. E ) FAK inhibition did not augment the dissociation-induced CNTF mRNA up-regulation. Data are fold change compared to controls and are shown as average +/− SEM. In A, n = 6, D and E, n = 4.
    Fak Inhibitor Pf573228 In Vehicle, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fak inhibitor pf573228 in vehicle/product/Tocris
    Average 93 stars, based on 1 article reviews
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    Tocris fak inhibitor pf
    Effects <t>of</t> <t>PF-228</t> on the phosphorylation of FAK, Akt and ERK in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 1 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, suspended AsPC-1 cells were plated onto plastic or LN-coated plates for 1 h in the continued presence or absence of PF-228. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.
    Fak Inhibitor Pf, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fak inhibitor pf/product/Tocris
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fak inhibitor pf - by Bioz Stars, 2024-02
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    93
    Tocris fak inhibitor
    Effects <t>of</t> <t>PF-228</t> on the phosphorylation of FAK, Akt and ERK in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 1 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, suspended AsPC-1 cells were plated onto plastic or LN-coated plates for 1 h in the continued presence or absence of PF-228. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.
    Fak Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fak inhibitor/product/Tocris
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    fak inhibitor - by Bioz Stars, 2024-02
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    Tocris pf 573228 fak inhibitor

    Pf 573228 Fak Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pf 573228 fak inhibitor/product/Tocris
    Average 93 stars, based on 1 article reviews
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    Rat VSMCs were treated with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.

    Journal: PLoS ONE

    Article Title: Periostin Links Mechanical Strain to Inflammation in Abdominal Aortic Aneurysm

    doi: 10.1371/journal.pone.0079753

    Figure Lengend Snippet: Rat VSMCs were treated with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) and stimulated with cyclic uniaxial strain (20% elongation in length at a frequency of 30 cycles/min for 48 h, n = 5). Levels of phosphorylated FAK ( A, B ), ERK ( A, C ) and JNK ( A, D ) in cell lysates were determined by western blotting. Protein levels of MCP-1 ( A, E ) and periostin ( A, G ) in cell lysates, and active MMP-2 ( A, F ) in conditioned media were determined by western blotting and gelatin zymography, respectively. GAPDH served as an internal control. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control; # p <0.05 and ## p <0.01 compared to VSMCs under mechanical strain.

    Article Snippet: For the inhibition study, 10 µM of FAK inhibitor (PF573228) (Tocris Bioscience) was added to the medium for 48 h.

    Techniques: Western Blot, Zymography

    Human AAA tissues were cut into small pieces and cultured with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) for 48 h (n = 5). Levels of phosphorylated FAK ( A ), ERK ( B ) and JNK ( C ) in tissue lysates were determined by western blotting. GAPDH served as an internal control. Protein levels of MCP-1 ( D ) and MMPs ( E-F ) in conditioned media were determined by ELISA and gelatin zymography, respectively. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control.

    Journal: PLoS ONE

    Article Title: Periostin Links Mechanical Strain to Inflammation in Abdominal Aortic Aneurysm

    doi: 10.1371/journal.pone.0079753

    Figure Lengend Snippet: Human AAA tissues were cut into small pieces and cultured with or without of FAK inhibitor (PF573228, 10 µM, FAK-I) for 48 h (n = 5). Levels of phosphorylated FAK ( A ), ERK ( B ) and JNK ( C ) in tissue lysates were determined by western blotting. GAPDH served as an internal control. Protein levels of MCP-1 ( D ) and MMPs ( E-F ) in conditioned media were determined by ELISA and gelatin zymography, respectively. Data are mean ± SE. * p <0.05 and ** p <0.01 compared to Control.

    Article Snippet: For the inhibition study, 10 µM of FAK inhibitor (PF573228) (Tocris Bioscience) was added to the medium for 48 h.

    Techniques: Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay, Zymography

    FAK inhibition induces CNTF expression in vitro. A ) C6 cells incubated for 4 hours with FAK inhibitor PF573228 have increased levels of CNTF mRNA compared to vehicle-only cultures (Ctrl). B ) The inhibitor reduced FAK activity as shown in Western blots by the reduced levels of phosphorylated FAK (pFAK tyr-397). C ) CNTF protein was also increased in the same protein extracts, showing the rapid and robust nature of the disinhibition. Blots are representative of 4 independent observations. D ) C6 cell injury by mechanical dissociation robustly induced CNTF mRNA within 2 hours which was lost by 6 hours. E ) FAK inhibition did not augment the dissociation-induced CNTF mRNA up-regulation. Data are fold change compared to controls and are shown as average +/− SEM. In A, n = 6, D and E, n = 4.

    Journal: Cell Communication and Signaling : CCS

    Article Title: Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression

    doi: 10.1186/1478-811X-11-35

    Figure Lengend Snippet: FAK inhibition induces CNTF expression in vitro. A ) C6 cells incubated for 4 hours with FAK inhibitor PF573228 have increased levels of CNTF mRNA compared to vehicle-only cultures (Ctrl). B ) The inhibitor reduced FAK activity as shown in Western blots by the reduced levels of phosphorylated FAK (pFAK tyr-397). C ) CNTF protein was also increased in the same protein extracts, showing the rapid and robust nature of the disinhibition. Blots are representative of 4 independent observations. D ) C6 cell injury by mechanical dissociation robustly induced CNTF mRNA within 2 hours which was lost by 6 hours. E ) FAK inhibition did not augment the dissociation-induced CNTF mRNA up-regulation. Data are fold change compared to controls and are shown as average +/− SEM. In A, n = 6, D and E, n = 4.

    Article Snippet: A total of 2.56 million C6 cells were plated at 160,000 cells/ml in 75 cm 2 flasks for 24 hours, then treated with vehicle (75% DMSO) or 10 μM FAK inhibitor PF573228 in vehicle (Cat. #3239, Tocris) for 4 hours.

    Techniques: Inhibition, Expressing, In Vitro, Incubation, Activity Assay, Western Blot

    FAK inhibition induces CNTF expression in the adult CNS in vivo . A ) Intracerebral injection of the FAK inhibitor PF573228 directly in the striatum of mice results in increased CNTF protein 4 hours later as shown in western blots. FAK activity was greatly decreased as shown by lack of phosphorylation. (pFAK). α-tubulin = internal control. Lanes are from individual animals. B ) Similar results were obtained after direct injection into the spinal cord. C ) Systemic intraperitoneal injections of the FAK inhibitors PF573228 or FAK14 induce CNTF mRNA in the spinal cord or SVZ relative to vehicle only injected mice (Ctrl). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are fold change compared to control (Ctrl) and are means +/− SEM from 3–4 mice per group.

    Journal: Cell Communication and Signaling : CCS

    Article Title: Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression

    doi: 10.1186/1478-811X-11-35

    Figure Lengend Snippet: FAK inhibition induces CNTF expression in the adult CNS in vivo . A ) Intracerebral injection of the FAK inhibitor PF573228 directly in the striatum of mice results in increased CNTF protein 4 hours later as shown in western blots. FAK activity was greatly decreased as shown by lack of phosphorylation. (pFAK). α-tubulin = internal control. Lanes are from individual animals. B ) Similar results were obtained after direct injection into the spinal cord. C ) Systemic intraperitoneal injections of the FAK inhibitors PF573228 or FAK14 induce CNTF mRNA in the spinal cord or SVZ relative to vehicle only injected mice (Ctrl). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are fold change compared to control (Ctrl) and are means +/− SEM from 3–4 mice per group.

    Article Snippet: A total of 2.56 million C6 cells were plated at 160,000 cells/ml in 75 cm 2 flasks for 24 hours, then treated with vehicle (75% DMSO) or 10 μM FAK inhibitor PF573228 in vehicle (Cat. #3239, Tocris) for 4 hours.

    Techniques: Inhibition, Expressing, In Vivo, Injection, Western Blot, Activity Assay

    Systemic FAK inhibitor treatment increases adult CNS neurogenesis. A ) mRNA for the proliferation marker Ki67 and the marker for proliferating neural C cell progenitors, EGFR, increased in the SVZ after systemic FAK inhibitor PF573228 treatment in mice. CNTF mRNA was increased in the same extracts (Figure 6C). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are mean fold change compared to vehicle treated mice +/− SEM, n = 3–4 mice. B ) In other mice, the systemic injections of FAK inhibitor PF573228 (n = 6) increased the number of DCX + neuroblasts in the SVZ. Data are expressed as a mean percentage of vehicle (n = 4) treated mice +/− SEM. C ) Representative confocal images of the dorsal SVZ showing increased numbers of nuclei (Hoechst) and neuroblasts (dcx) in an FAK inhibitor-injected mouse compared to a vehicle-injected mouse. Scale bar = 20 μm. CC = corpus callosum, LV = lateral ventricle, STR = striatum.

    Journal: Cell Communication and Signaling : CCS

    Article Title: Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression

    doi: 10.1186/1478-811X-11-35

    Figure Lengend Snippet: Systemic FAK inhibitor treatment increases adult CNS neurogenesis. A ) mRNA for the proliferation marker Ki67 and the marker for proliferating neural C cell progenitors, EGFR, increased in the SVZ after systemic FAK inhibitor PF573228 treatment in mice. CNTF mRNA was increased in the same extracts (Figure 6C). Drugs were given 3 times every 24 hours and tissues collected 2 hours after the last injection. Data are mean fold change compared to vehicle treated mice +/− SEM, n = 3–4 mice. B ) In other mice, the systemic injections of FAK inhibitor PF573228 (n = 6) increased the number of DCX + neuroblasts in the SVZ. Data are expressed as a mean percentage of vehicle (n = 4) treated mice +/− SEM. C ) Representative confocal images of the dorsal SVZ showing increased numbers of nuclei (Hoechst) and neuroblasts (dcx) in an FAK inhibitor-injected mouse compared to a vehicle-injected mouse. Scale bar = 20 μm. CC = corpus callosum, LV = lateral ventricle, STR = striatum.

    Article Snippet: A total of 2.56 million C6 cells were plated at 160,000 cells/ml in 75 cm 2 flasks for 24 hours, then treated with vehicle (75% DMSO) or 10 μM FAK inhibitor PF573228 in vehicle (Cat. #3239, Tocris) for 4 hours.

    Techniques: Marker, Injection

    Effects of PF-228 on the phosphorylation of FAK, Akt and ERK in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 1 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, suspended AsPC-1 cells were plated onto plastic or LN-coated plates for 1 h in the continued presence or absence of PF-228. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.

    Journal: Molecular Cancer

    Article Title: Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines

    doi: 10.1186/1476-4598-8-125

    Figure Lengend Snippet: Effects of PF-228 on the phosphorylation of FAK, Akt and ERK in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 1 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, suspended AsPC-1 cells were plated onto plastic or LN-coated plates for 1 h in the continued presence or absence of PF-228. Western blot showed expression of pFAK (pY397), p-Akt (pS473), p-ERK 1/2 and their total proteins in the cells. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.

    Article Snippet: The FAK inhibitor PF-573,228 was purchased from Tocris (Bristol, UK).

    Techniques: Western Blot, Expressing

    Effects of PF-228 on Gem-induced apoptosis in Panc-1 cells . Panc-1 cells were pretreated with or without PF-228 (1 μM) for 1 h and then treated with or without 10 μM Gem for 72 h in the continued presence or absence of PF-228. The cells were subjected to apoptosis analysis by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling (B) and western blot analysis of cleaved caspase-3 protein expression (C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. Panc-1 cells without Gem treatment; #, P < 0.05, vs. Gem-treated Panc-1 cells without PF-228 pretreatment.

    Journal: Molecular Cancer

    Article Title: Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines

    doi: 10.1186/1476-4598-8-125

    Figure Lengend Snippet: Effects of PF-228 on Gem-induced apoptosis in Panc-1 cells . Panc-1 cells were pretreated with or without PF-228 (1 μM) for 1 h and then treated with or without 10 μM Gem for 72 h in the continued presence or absence of PF-228. The cells were subjected to apoptosis analysis by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling (B) and western blot analysis of cleaved caspase-3 protein expression (C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. Panc-1 cells without Gem treatment; #, P < 0.05, vs. Gem-treated Panc-1 cells without PF-228 pretreatment.

    Article Snippet: The FAK inhibitor PF-573,228 was purchased from Tocris (Bristol, UK).

    Techniques: Staining, Flow Cytometry, Labeling, Western Blot, Expressing

    Effects of PF-228 on LN-mediated Gem chemoresistance in AsPC-1 cells . After pretreated with or without PF-228 (1 μM) for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN-coated 6-well plates and treated with Gem (0.1 μM) for 72 h in the continued presence or absence of PF-228. Cell apoptosis was examined by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling(B) and western blot analysis of cleaved caspase-3 protein expression(C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. AsPC-1 cells without Gem treatment; #, P < 0.05, vs. Gem-treated AsPC-1 cells without PF-228 pretreatment on LN.

    Journal: Molecular Cancer

    Article Title: Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines

    doi: 10.1186/1476-4598-8-125

    Figure Lengend Snippet: Effects of PF-228 on LN-mediated Gem chemoresistance in AsPC-1 cells . After pretreated with or without PF-228 (1 μM) for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN-coated 6-well plates and treated with Gem (0.1 μM) for 72 h in the continued presence or absence of PF-228. Cell apoptosis was examined by Hoechst staining (the arrow indicates the apoptotic cells) (A), flow cytometry analysis of Annexin-V labeling(B) and western blot analysis of cleaved caspase-3 protein expression(C). Bars represent the mean of three independent experiments ± SE. *, P < 0.05, vs. AsPC-1 cells without Gem treatment; #, P < 0.05, vs. Gem-treated AsPC-1 cells without PF-228 pretreatment on LN.

    Article Snippet: The FAK inhibitor PF-573,228 was purchased from Tocris (Bristol, UK).

    Techniques: Staining, Flow Cytometry, Labeling, Western Blot, Expressing

    Effects of PF-228 on the expression of apoptosis-associated proteins in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 24 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN for 24 h in the continued presence or absence of PF-228. Western blot analysis was used to detect the expression of Bad, p-Bad (pS136), p-Bad (pS112), Bcl-2, Bax and survivin. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.

    Journal: Molecular Cancer

    Article Title: Intrinsic chemoresistance to gemcitabine is associated with constitutive and laminin-induced phosphorylation of FAK in pancreatic cancer cell lines

    doi: 10.1186/1476-4598-8-125

    Figure Lengend Snippet: Effects of PF-228 on the expression of apoptosis-associated proteins in pancreatic cancer cells . A, Panc-1 cells were treated with or without the indicated concentrations of PF-228 for 24 h. B, After pretreated with or without the indicated concentrations of PF-228 for 30 min in serum-free medium, AsPC-1 cells were plated onto plastic or LN for 24 h in the continued presence or absence of PF-228. Western blot analysis was used to detect the expression of Bad, p-Bad (pS136), p-Bad (pS112), Bcl-2, Bax and survivin. The membranes were probed with anti-β-actin antibody to ensure even loading of proteins in each lane.

    Article Snippet: The FAK inhibitor PF-573,228 was purchased from Tocris (Bristol, UK).

    Techniques: Expressing, Western Blot

    Journal: Cell Reports Medicine

    Article Title: C-MORE: A high-content single-cell morphology recognition methodology for liquid biopsies toward personalized cardiovascular medicine

    doi: 10.1016/j.xcrm.2021.100436

    Figure Lengend Snippet:

    Article Snippet: PF 573228 FAK inhibitor , Tocris , 3239.

    Techniques: Recombinant, Electron Microscopy, Software, Imaging