Journal: Liver Research

Article Title: Efficient AAV8 delivery to the liver via isolated hepatic perfusion and analysis of hepatic lobule transduction patterns

doi: 10.1016/j.livres.2025.04.006

Figure Lengend Snippet: IHP efficiently delivers AAV8 to each lobe of the rat liver. (A) Fluorescence distribution in each liver lobe. (B) Area fraction of dTomato + cells in each liver lobe. (C) Average fluorescence intensity of dTomato + cells in each liver lobe. L, left lateral; LM, left medial; M, middle; RL, right lower; RU, right upper; T, tail. (D) Representative images of hepatic cell marker FAH and dTomato co-staining in areas with dTomato high and low expression levels. Five images per rat ( n = 4) used for statistical analysis. ∗∗∗∗ P < 0.0001. Abbreviations: AAV8, adeno-associated virus 8; DAPI, 4′,6-diamidino-2-phenylindole; FAH, fumarylacetoacetate hydrolase; IHP, isolated hepatic perfusion.

Article Snippet: The primary antibodies glutamine synthetase (GS, #ab176562, Abcam, Cambridge, MA, USA) and fumarylacetoacetate hydrolase (FAH, #A13492, ABclonal Biotechnology Co., Ltd, Wuhan, China) were diluted at a ratio of 1:200 and then incubated overnight at 4 °C.

Techniques: Fluorescence, Marker, Staining, Expressing, Virus, Isolation

Journal: Liver Research

Article Title: Efficient AAV8 delivery to the liver via isolated hepatic perfusion and analysis of hepatic lobule transduction patterns

doi: 10.1016/j.livres.2025.04.006

Figure Lengend Snippet: AAV8 delivery by IHP exhibits partial dose dependence and uneven distribution characteristics in the rat liver. (A) Fluorescence distribution under different dose settings (2 × 10 12 GC/kg, 4 × 10 12 GC/kg, and 7 × 10 12 GC/kg, respectively). (B) Area fraction of fluorescence expression in the unit field of view of liver tissue slices under different dose settings. (C) Mean fluorescence intensity in the unit field of view of liver tissue slices under different dose settings. (D) Representative images of hepatic cell marker FAH and dTomato co-staining under different dose settings. (E) The proportion of FAH and dTomato co-stained cells in FAH + hepatocytes under different dose settings. n = 4 per group, ∗ P < 0.05, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. ns, not significant. Abbreviations: AAV8, adeno-associated virus 8; DAPI, 4′,6-diamidino-2-phenylindole; FAH, fumarylacetoacetate hydrolase; GC, genome copies; IHP, isolated hepatic perfusion.

Article Snippet: The primary antibodies glutamine synthetase (GS, #ab176562, Abcam, Cambridge, MA, USA) and fumarylacetoacetate hydrolase (FAH, #A13492, ABclonal Biotechnology Co., Ltd, Wuhan, China) were diluted at a ratio of 1:200 and then incubated overnight at 4 °C.

Techniques: Fluorescence, Expressing, Marker, Staining, Virus, Isolation

Journal: bioRxiv

Article Title: 3-Phenylpropionic acid, a microbiota-derived polyphenol metabolite linked to hippuric acid, ameliorates colitis as a colon-enriched PPAR-γ agonist

doi: 10.1101/2025.11.25.690607

Figure Lengend Snippet: (A) Screening of commensal and probiotic strains for 3-PPA production. Inset: Polyphenol supplementation inhibited bacterial growth. Mean ± SEM. (B) Chromatogram showing healthy subject isolate E. coli FAH degraded CGA. (C) Colonisation of E. coli FAH raised plasma HIPA levels but not PAGly. FMT: faecal microbiota transplantation using faeces from conventionally raised mice. Mean ± SD; n=4–5. (D) C. sporogenes , B. breve and E. coli FAH converted trans-cinnamic acid to 3-PPA. Mean ± SEM; n=3. (E) Pharmacokinetic profiling of trans-cinnamic acid and related metabolites following oral administration. Mean ± SD; n=3-5. Significance was assessed by two-way ANOVA, with P-values between lines indicating treatment effects. (F-H) Colonization with 3-PPA-producing bacteria increased 3-PPA and HIPA levels in vivo . (F) Study design. Plasma, urinary, and faecal concentrations of 3-PPA (G) and HIPA (H) were assessed following bacterial colonization in vivo . Significance was assessed using the Kruskal– Wallis test followed by pairwise comparisons without correction for multiple testing. Mean ± SD; n=3–6. See also Figure S9.

Article Snippet: E. coli FAH was deposited at National Center for Biotechnology Information (NCBI: PRJNA923252).

Techniques: Clinical Proteomics, Transplantation Assay, Bacteria, In Vivo