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Becton Dickinson facscan laser flow cytometry analysis system
Facscan Laser Flow Cytometry Analysis System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/facscan laser flow cytometry analysis system/product/Becton Dickinson
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
facscan laser flow cytometry analysis system - by Bioz Stars, 2020-11
93/100 stars

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dna content
propidium iodide

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Staining:

Article Title: Study of the antitumor mechanisms of apiole derivatives (AP-02) from Petroselinum crispum through induction of G0/G1 phase cell cycle arrest in human COLO 205 cancer cells
Article Snippet: .. Cells were stained with propidium iodide (50 μg/ml) (Sigma Chemical Co., St. Louis, MO, USA), and DNA content was assessed using a FACScan laser flow cytometry analysis system (Becton-Dickinson, San Jose, CA, USA), with 15,000 events being analyzed for each sample. .. Western blot analysis Cell lysates were prepared, electrotransferred, immunoblotted with antibodies, and then visualized via incubation with colorigenic substrates (nitroblue tetrazolium, NBT, and 5-bromo-4-chloro-3-indolyl phosphate, BCIP) (Sigma Chemical Co., St. Louis, MO, USA).

Flow Cytometry:

Article Title: Study of the antitumor mechanisms of apiole derivatives (AP-02) from Petroselinum crispum through induction of G0/G1 phase cell cycle arrest in human COLO 205 cancer cells
Article Snippet: .. Cells were stained with propidium iodide (50 μg/ml) (Sigma Chemical Co., St. Louis, MO, USA), and DNA content was assessed using a FACScan laser flow cytometry analysis system (Becton-Dickinson, San Jose, CA, USA), with 15,000 events being analyzed for each sample. .. Western blot analysis Cell lysates were prepared, electrotransferred, immunoblotted with antibodies, and then visualized via incubation with colorigenic substrates (nitroblue tetrazolium, NBT, and 5-bromo-4-chloro-3-indolyl phosphate, BCIP) (Sigma Chemical Co., St. Louis, MO, USA).

Cytometry:

Article Title: Study of the antitumor mechanisms of apiole derivatives (AP-02) from Petroselinum crispum through induction of G0/G1 phase cell cycle arrest in human COLO 205 cancer cells
Article Snippet: .. Cells were stained with propidium iodide (50 μg/ml) (Sigma Chemical Co., St. Louis, MO, USA), and DNA content was assessed using a FACScan laser flow cytometry analysis system (Becton-Dickinson, San Jose, CA, USA), with 15,000 events being analyzed for each sample. .. Western blot analysis Cell lysates were prepared, electrotransferred, immunoblotted with antibodies, and then visualized via incubation with colorigenic substrates (nitroblue tetrazolium, NBT, and 5-bromo-4-chloro-3-indolyl phosphate, BCIP) (Sigma Chemical Co., St. Louis, MO, USA).

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  • 92
    Becton Dickinson fluorescence activated cell sorting analysis
    Effect of IS (15–60 μM) on ROS formation (A) , evaluated by means of the probe H 2 DCF-DA, in astrocytes and mixed glial cells. Cellular <t>fluorescence</t> was evaluated using <t>fluorescence-activated</t> <t>cell</t> <t>sorting</t> <t>analysis</t> (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (15–60 μM) on nitrotyrosine formmation (B) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (30 μM) on Nrf2 nuclear translocation in astrocytes and mixed glial cells (C) . Nuclear translocation of Nrf2 was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of nucleus (DAPI) and Nrf2, respectively. Analysis was performed by confocal laser scanning microscopy. Effect of IS (15–60 μM) on HO-1 expression (D) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 9). ∘∘∘ , ∘∘ , and °denote P
    Fluorescence Activated Cell Sorting Analysis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence activated cell sorting analysis/product/Becton Dickinson
    Average 92 stars, based on 219 article reviews
    Price from $9.99 to $1999.99
    fluorescence activated cell sorting analysis - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

    94
    Becton Dickinson facscan flow cytometer
    Effect of IS (15–60 μM) on ROS formation (A) , evaluated by means of the probe H 2 DCF-DA, in astrocytes and mixed glial cells. Cellular <t>fluorescence</t> was evaluated using <t>fluorescence-activated</t> <t>cell</t> <t>sorting</t> <t>analysis</t> (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (15–60 μM) on nitrotyrosine formmation (B) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (30 μM) on Nrf2 nuclear translocation in astrocytes and mixed glial cells (C) . Nuclear translocation of Nrf2 was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of nucleus (DAPI) and Nrf2, respectively. Analysis was performed by confocal laser scanning microscopy. Effect of IS (15–60 μM) on HO-1 expression (D) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 9). ∘∘∘ , ∘∘ , and °denote P
    Facscan Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facscan flow cytometer/product/Becton Dickinson
    Average 94 stars, based on 512 article reviews
    Price from $9.99 to $1999.99
    facscan flow cytometer - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

    86
    Becton Dickinson facscan laser flow cytometer analysis system
    Effect of IS (15–60 μM) on ROS formation (A) , evaluated by means of the probe H 2 DCF-DA, in astrocytes and mixed glial cells. Cellular <t>fluorescence</t> was evaluated using <t>fluorescence-activated</t> <t>cell</t> <t>sorting</t> <t>analysis</t> (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (15–60 μM) on nitrotyrosine formmation (B) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (30 μM) on Nrf2 nuclear translocation in astrocytes and mixed glial cells (C) . Nuclear translocation of Nrf2 was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of nucleus (DAPI) and Nrf2, respectively. Analysis was performed by confocal laser scanning microscopy. Effect of IS (15–60 μM) on HO-1 expression (D) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 9). ∘∘∘ , ∘∘ , and °denote P
    Facscan Laser Flow Cytometer Analysis System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facscan laser flow cytometer analysis system/product/Becton Dickinson
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    facscan laser flow cytometer analysis system - by Bioz Stars, 2020-11
    86/100 stars
      Buy from Supplier

    90
    Becton Dickinson facscan laser flow cytometry analysis
    Effect of IS (15–60 μM) on ROS formation (A) , evaluated by means of the probe H 2 DCF-DA, in astrocytes and mixed glial cells. Cellular <t>fluorescence</t> was evaluated using <t>fluorescence-activated</t> <t>cell</t> <t>sorting</t> <t>analysis</t> (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (15–60 μM) on nitrotyrosine formmation (B) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (30 μM) on Nrf2 nuclear translocation in astrocytes and mixed glial cells (C) . Nuclear translocation of Nrf2 was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of nucleus (DAPI) and Nrf2, respectively. Analysis was performed by confocal laser scanning microscopy. Effect of IS (15–60 μM) on HO-1 expression (D) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 9). ∘∘∘ , ∘∘ , and °denote P
    Facscan Laser Flow Cytometry Analysis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facscan laser flow cytometry analysis/product/Becton Dickinson
    Average 90 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    facscan laser flow cytometry analysis - by Bioz Stars, 2020-11
    90/100 stars
      Buy from Supplier

    Image Search Results


    Effect of IS (15–60 μM) on ROS formation (A) , evaluated by means of the probe H 2 DCF-DA, in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (15–60 μM) on nitrotyrosine formmation (B) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (30 μM) on Nrf2 nuclear translocation in astrocytes and mixed glial cells (C) . Nuclear translocation of Nrf2 was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of nucleus (DAPI) and Nrf2, respectively. Analysis was performed by confocal laser scanning microscopy. Effect of IS (15–60 μM) on HO-1 expression (D) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 9). ∘∘∘ , ∘∘ , and °denote P

    Journal: Frontiers in Pharmacology

    Article Title: Indoxyl Sulfate Affects Glial Function Increasing Oxidative Stress and Neuroinflammation in Chronic Kidney Disease: Interaction between Astrocytes and Microglia

    doi: 10.3389/fphar.2017.00370

    Figure Lengend Snippet: Effect of IS (15–60 μM) on ROS formation (A) , evaluated by means of the probe H 2 DCF-DA, in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (15–60 μM) on nitrotyrosine formmation (B) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (30 μM) on Nrf2 nuclear translocation in astrocytes and mixed glial cells (C) . Nuclear translocation of Nrf2 was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of nucleus (DAPI) and Nrf2, respectively. Analysis was performed by confocal laser scanning microscopy. Effect of IS (15–60 μM) on HO-1 expression (D) in astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 9). ∘∘∘ , ∘∘ , and °denote P

    Article Snippet: Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software.

    Techniques: Fluorescence, FACS, Software, Translocation Assay, Immunofluorescence, Confocal Microscopy, Confocal Laser Scanning Microscopy, Expressing

    Effect of IS (15–60 μM) on iNOS (A) , COX-2 (B) expression by astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 9). Effect of IS (15–60 μM) TNF-α (C) and IL-6 (D) release by astrocytes and mixed glial cells ( n = 9). Cyokine release was assessed by ELISA assay and expressed as pg/ml ( n = 9). ∘∘∘ , ∘∘ , and °denote P

    Journal: Frontiers in Pharmacology

    Article Title: Indoxyl Sulfate Affects Glial Function Increasing Oxidative Stress and Neuroinflammation in Chronic Kidney Disease: Interaction between Astrocytes and Microglia

    doi: 10.3389/fphar.2017.00370

    Figure Lengend Snippet: Effect of IS (15–60 μM) on iNOS (A) , COX-2 (B) expression by astrocytes and mixed glial cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 9). Effect of IS (15–60 μM) TNF-α (C) and IL-6 (D) release by astrocytes and mixed glial cells ( n = 9). Cyokine release was assessed by ELISA assay and expressed as pg/ml ( n = 9). ∘∘∘ , ∘∘ , and °denote P

    Article Snippet: Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software.

    Techniques: Expressing, Fluorescence, FACS, Software, Enzyme-linked Immunosorbent Assay

    Effect of IS (15–60 μM) on ROS formation (A) , evaluated by means of the probe H 2 DCF-DA, in C6 cells in presence of DPI and of NAC. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 12). Effect of IS (30 μM) on Nrf2 nuclear translocation in C6 cells in presence of DPI and NAC (B) . Nuclear translocation of Nrf2 was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of the nucleus (DAPI) and Nrf2, respectively. Analysis was performed by confocal laser scanning microscopy. Effect of IS (15–60 μM) on HO-1 (C) , NQO1 (D) , and SOD (E) expression in C6 cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (30 μM) on AhR nuclear translocation in presence of DPI in C6 cells (F) . Nuclear translocation of AhR was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of nucleus (DAPI) and AhR, respectively. Analysis was performed by confocal laser scanning microscopy and values are expressed as mean fluorescence intensity ( n = 12). Effect of IS (15–60 μM) on ROS formation (G) , evaluated by means of the probe H 2 DCF-DA, in C6 cells in presence of CH-223191. Values are expressed as mean fluorescence intensity ( n = 9). ∘∘∘ , ∘∘ , and °denote P

    Journal: Frontiers in Pharmacology

    Article Title: Indoxyl Sulfate Affects Glial Function Increasing Oxidative Stress and Neuroinflammation in Chronic Kidney Disease: Interaction between Astrocytes and Microglia

    doi: 10.3389/fphar.2017.00370

    Figure Lengend Snippet: Effect of IS (15–60 μM) on ROS formation (A) , evaluated by means of the probe H 2 DCF-DA, in C6 cells in presence of DPI and of NAC. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Values are expressed as mean fluorescence intensity ( n = 12). Effect of IS (30 μM) on Nrf2 nuclear translocation in C6 cells in presence of DPI and NAC (B) . Nuclear translocation of Nrf2 was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of the nucleus (DAPI) and Nrf2, respectively. Analysis was performed by confocal laser scanning microscopy. Effect of IS (15–60 μM) on HO-1 (C) , NQO1 (D) , and SOD (E) expression in C6 cells. Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software. Effect of IS (30 μM) on AhR nuclear translocation in presence of DPI in C6 cells (F) . Nuclear translocation of AhR was detected using immunofluorescence confocal microscopy. Scale bar, 10 μm. Blue and green fluorescences indicate localization of nucleus (DAPI) and AhR, respectively. Analysis was performed by confocal laser scanning microscopy and values are expressed as mean fluorescence intensity ( n = 12). Effect of IS (15–60 μM) on ROS formation (G) , evaluated by means of the probe H 2 DCF-DA, in C6 cells in presence of CH-223191. Values are expressed as mean fluorescence intensity ( n = 9). ∘∘∘ , ∘∘ , and °denote P

    Article Snippet: Cellular fluorescence was evaluated using fluorescence-activated cell sorting analysis (FACSscan; Becton Dickinson) and elaborated with Cell Quest software.

    Techniques: Fluorescence, FACS, Software, Translocation Assay, Immunofluorescence, Confocal Microscopy, Confocal Laser Scanning Microscopy, Expressing