Structured Review

Becton Dickinson facsaria
Facsaria, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/facsaria/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
facsaria - by Bioz Stars, 2021-05
86/100 stars

Images

Related Articles

Staining:

Article Title: The cell cycle restricts activation-induced cytidine deaminase activity to early G1
Article Snippet: Where indicated, nocodazole (100 ng/ml; Sigma-Aldrich) was added at 42 h after isolation for synchronization. .. After 6 h of nocodazole treatment, cells were washed and released in nocodazole-free supplemented RPMI for 0–6 h. Cells were then collected and fixed with 4.2% formaldehyde at room temperature for 20 min. After DAPI staining (0.5 µg/ml) at room temperature for 5 min, cells were sorted using a FACSAria (BD). .. The cytokinesis population was determined by combining DNA fluorescence peak (DAPI-H) and intensity (DAPI-A) according to a previous study ( ).

Article Title: Isolation of Plasmodium falciparum by flow-cytometry: implications for single-trophozoite genotyping and parasite DNA purification for whole-genome high-throughput sequencing of archival samples
Article Snippet: Characterization of nucleus-stained trophozoites by flow cytometry DNA was stained with Propidium Iodide (PI) at a 10 μg/mL final concentration [ ] in a 1.5 mL microtube and kept for at least 1 h in the dark. .. The stained samples were then analysed with a FACSAria (Becton-Dickinson, Mountain View, CA). .. The PI-stained nuclei were excited with a 488 nm light and were gated on the basis of their fluorescence intensity (FI) and forward scatter (FSC) [ ].

Flow Cytometry:

Article Title: BCR-ABL1 and CD66c exhibit high concordance in minimal residual disease detection of adult B-acute lymphoblastic leukemia
Article Snippet: .. Data were acquired and analyzed by flow cytometry on the FACSAria I or II with Diva software (BD Biosciences; San Jose, CA, USA). .. Surface antigens were considered as positively expressed when > 20% of analyzed events were stained while a cutoff of > 10% was set for cytoplasmic antigens.

Article Title: Composing a Tumor Specific Bacterial Promoter
Article Snippet: The homogenates were diluted 1:10 (spleen, liver) or 1:100 (tumors) in 0.1% TritonX-100/PBS containing 2 mM EDTA, filtered through a 30 μm CellTrics filter (Partec, Germany) and sorted. .. Samples were analyzed via two color flow cytometry on a FACSAria or LSRII, respectively (Becton Dickinson, USA) and plated on LB plates containing 50 μg/ml ampicillin to allow normalization. ..

Article Title: Loss of Heterozygosity and Copy Number Alterations in Flow-Sorted Bulky Cervical Cancer
Article Snippet: The WinList 6.0 and ModFit 3.2.1 software packages (Verity Software House, Inc., Topsham, ME) were used for data analysis and DNA index (DI) calculation (median of G0 G1 population of tumor cell fraction / median of G0 G1 population of stromal cell fraction). .. In order to separate tumor cells from genetically normal cells (connective tissue, lymphocyes and blood vessels) , . keratin-positive and vimentin-positive normal cells were collected separately, using a FACSAria I flow-sorter at 40 psi (BD Biosciences, Erembodegem, Belgium). .. In cases where flow cytometry detected more than one population of keratin-positive tumor cells, both populations were sorted independently.

Cytometry:

Article Title: BCR-ABL1 and CD66c exhibit high concordance in minimal residual disease detection of adult B-acute lymphoblastic leukemia
Article Snippet: .. Data were acquired and analyzed by flow cytometry on the FACSAria I or II with Diva software (BD Biosciences; San Jose, CA, USA). .. Surface antigens were considered as positively expressed when > 20% of analyzed events were stained while a cutoff of > 10% was set for cytoplasmic antigens.

Article Title: Composing a Tumor Specific Bacterial Promoter
Article Snippet: The homogenates were diluted 1:10 (spleen, liver) or 1:100 (tumors) in 0.1% TritonX-100/PBS containing 2 mM EDTA, filtered through a 30 μm CellTrics filter (Partec, Germany) and sorted. .. Samples were analyzed via two color flow cytometry on a FACSAria or LSRII, respectively (Becton Dickinson, USA) and plated on LB plates containing 50 μg/ml ampicillin to allow normalization. ..

Software:

Article Title: BCR-ABL1 and CD66c exhibit high concordance in minimal residual disease detection of adult B-acute lymphoblastic leukemia
Article Snippet: .. Data were acquired and analyzed by flow cytometry on the FACSAria I or II with Diva software (BD Biosciences; San Jose, CA, USA). .. Surface antigens were considered as positively expressed when > 20% of analyzed events were stained while a cutoff of > 10% was set for cytoplasmic antigens.

Expressing:

Article Title: IFNγ Signaling Endows DCs with the Capacity to Control Type I Inflammation during Parasitic Infection through Promoting T-bet+ Regulatory T Cells
Article Snippet: Generation of bone marrow chimeras Mixed bone marrow chimeras were generated by transferring BM cells from CD11c cre IFNγR2 fl/fl or IL-27Rα -/- mice and C57BL6 Ly5.1 mice as 1:1 ratio into lethally irradiated Rag1 -/- recipients, as described previously [ ]. .. Gene expression profiling analysis For microarray analysis, CD11c+ MHC class IIhi DCs from CD11c cre IFNγR2 fl/fl or WT littermates or from mixed BM chimeras were sorted on FACSAria (Becton Dickinson). .. Isolated RNA was subject to gene expression profiling analysis using Mouse Gene 2.0 ST array (Affymetrix).

Microarray:

Article Title: IFNγ Signaling Endows DCs with the Capacity to Control Type I Inflammation during Parasitic Infection through Promoting T-bet+ Regulatory T Cells
Article Snippet: Generation of bone marrow chimeras Mixed bone marrow chimeras were generated by transferring BM cells from CD11c cre IFNγR2 fl/fl or IL-27Rα -/- mice and C57BL6 Ly5.1 mice as 1:1 ratio into lethally irradiated Rag1 -/- recipients, as described previously [ ]. .. Gene expression profiling analysis For microarray analysis, CD11c+ MHC class IIhi DCs from CD11c cre IFNγR2 fl/fl or WT littermates or from mixed BM chimeras were sorted on FACSAria (Becton Dickinson). .. Isolated RNA was subject to gene expression profiling analysis using Mouse Gene 2.0 ST array (Affymetrix).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Becton Dickinson facsaria
    Proliferation and trans–well co–culture assays of CD25– and 3G11–expressing T cell subpopulations Splenocytes of naïve C57BL/6 mice were stained with anti-3G11 and anti-CD25 mAbs. CD25 + 3G11 − , CD25 + 3G11 + , CD25 − 3G11 − , CD25 − 3G11 + cells were sorted by BD <t>FACSAria</t> sorter (purity > 96%). ( A ) Separated cells were cultured (1×10 6 /ml) for 72 h in the presence or absence of 1.0 μg/ml anti-CD3. After 60 h of incubation, the cells were pulsed for 12 h with 1 μCi of [ 3 H]-methylthymidine. ( B ) To determine the immunosuppressive effect of these T cell subpopulations, they were co-cultured at a 1:1 ratio with anti-CD3 stimulated CD25 − CD4 + effector T cells of naïve C57BL/6 mice (both at 1×10 6 /ml), either as a mixed suspension or separated in transwell plates. Proliferative responses were determined in co-cultures with or without transwells. The proliferative responses are those of the CD25 − CD4 + effector cells in the lower wells of a transwell. ( C ) Different CD25- and 3G11-related T cell subpopulations from IL-10 −/− mice and their wild type control were purified by FACS sorting and were co-cultured with anti-CD3 stimulated CD25 − CD4 + effector T cells (both at 1×10 6 /ml). Columns refer to mean values and bars to SD. * represents the comparison of CD25 − CD4 + effector T cells only with other groups; @ represents the comparison of CD25 − 3G11 + T cells with other groups; and # represents the comparison of IL-10 −/− mice with wild type groups. *, p
    Facsaria, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facsaria/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    facsaria - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson facsaria iii cell sorter
    Repression of activation-induced cytidine deaminase (AID) induction in germinal center B cells (GCBs) from Pten fl/fl Cγ1 Cre/+ mice. (A) Flow cytometry sorting of in vitro induced IgM-BCR expressing splenic B cells from Pten +/+ Cγ1 Cre/+ and Pten fl/fl Cγ1 Cre/+ mice. Pure splenic B cells from control and KO mice (three mice for each group) were stimulated with LPS plus interleukin-4 (IL-4) for 4 days before sorting. (B) The expression levels of PTEN, p-AKT, p-FoxO1/3a, and AID were determined in Pten +/+ Cγ1 Cre/+ and Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells (left). Reduced PTEN, AID expression and enhanced p-AKT and p-FoxO1/3a expression were observed in Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells. The in vitro induced IgM-BCR expressing B cells were isolated by the <t>FACSAria</t> <t>III</t> Cell Sorter as described in (A) . Statistical comparison of protein expression level was also shown in right. Ratio values of different proteins from Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells were normalized to that of the Pten +/+ Cγ1 Cre/+ IgM-BCR expressing B cells. Data were given from one representative of at least three independent experiments. * p
    Facsaria Iii Cell Sorter, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facsaria iii cell sorter/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    facsaria iii cell sorter - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson sorp facsaria iii
    Verification of Muse cells after <t>FACSAria</t> <t>III</t> separation. A. Percentage of Muse cells among MSCs. B. Percentage of Muse cells within the positive fraction. C. Percentage of non-Muse cells within the negative fraction.
    Sorp Facsaria Iii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sorp facsaria iii/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sorp facsaria iii - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson bd facsaria ii
    Adjuvanticity of FlgE to Soluble Antigen in Mice. ( A–C ) Naïve OVA-specific T cells were purified from the spleens and inguinal lymph nodes of CD45.1 × OTII F1 mice by sorting using various antibodies to surface markers, labeled with eFluor450 and transferred into female C57BL/6 mice at 1 × 10 6 cells/mouse via tail vein injection. Twenty-four hours later, the recipients were treated or immunized via subcutaneous injection at the base of the tail with one of eight compositions, namely 1 μg OVA, 50 μg CpG-1826, 50 μg FlgE, 50 μg FlgEM each alone, or OVA plus the three stimulants individually. Control groups received 100 μL PBS. Three days later, the mice were sacrificed, and the draining inguinal lymph nodes were isolated and photographed ( A ). Single-cell suspension were made for staining with CD45.1 plus CD4 and then run on a BD <t>FACSAria</t> II and analyzed for eFluor450 intensity to quantify the proliferation of OVA-specific T cells ( B,C ). ( D ) To measure the humoral response, WT C57BL/6 mice were immunized with above 8 compositions except for increasing OVA doses to 100 μg. Two weeks later, the mice were sacrificed for serum harvest, and anti-OVA titers were measured using an ELISA as described in Materials and Methods. Data are representatives of two independent experiments that showed similar results. n = 3 mice for panel A–C, and n = 4 mice for panel D. *P
    Bd Facsaria Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bd facsaria ii/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bd facsaria ii - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Proliferation and trans–well co–culture assays of CD25– and 3G11–expressing T cell subpopulations Splenocytes of naïve C57BL/6 mice were stained with anti-3G11 and anti-CD25 mAbs. CD25 + 3G11 − , CD25 + 3G11 + , CD25 − 3G11 − , CD25 − 3G11 + cells were sorted by BD FACSAria sorter (purity > 96%). ( A ) Separated cells were cultured (1×10 6 /ml) for 72 h in the presence or absence of 1.0 μg/ml anti-CD3. After 60 h of incubation, the cells were pulsed for 12 h with 1 μCi of [ 3 H]-methylthymidine. ( B ) To determine the immunosuppressive effect of these T cell subpopulations, they were co-cultured at a 1:1 ratio with anti-CD3 stimulated CD25 − CD4 + effector T cells of naïve C57BL/6 mice (both at 1×10 6 /ml), either as a mixed suspension or separated in transwell plates. Proliferative responses were determined in co-cultures with or without transwells. The proliferative responses are those of the CD25 − CD4 + effector cells in the lower wells of a transwell. ( C ) Different CD25- and 3G11-related T cell subpopulations from IL-10 −/− mice and their wild type control were purified by FACS sorting and were co-cultured with anti-CD3 stimulated CD25 − CD4 + effector T cells (both at 1×10 6 /ml). Columns refer to mean values and bars to SD. * represents the comparison of CD25 − CD4 + effector T cells only with other groups; @ represents the comparison of CD25 − 3G11 + T cells with other groups; and # represents the comparison of IL-10 −/− mice with wild type groups. *, p

    Journal: Journal of the neurological sciences

    Article Title: Expression of 3G11 epitope defines subpopulations of regulatory T cells with different suppressive potency

    doi: 10.1016/j.jns.2010.04.019

    Figure Lengend Snippet: Proliferation and trans–well co–culture assays of CD25– and 3G11–expressing T cell subpopulations Splenocytes of naïve C57BL/6 mice were stained with anti-3G11 and anti-CD25 mAbs. CD25 + 3G11 − , CD25 + 3G11 + , CD25 − 3G11 − , CD25 − 3G11 + cells were sorted by BD FACSAria sorter (purity > 96%). ( A ) Separated cells were cultured (1×10 6 /ml) for 72 h in the presence or absence of 1.0 μg/ml anti-CD3. After 60 h of incubation, the cells were pulsed for 12 h with 1 μCi of [ 3 H]-methylthymidine. ( B ) To determine the immunosuppressive effect of these T cell subpopulations, they were co-cultured at a 1:1 ratio with anti-CD3 stimulated CD25 − CD4 + effector T cells of naïve C57BL/6 mice (both at 1×10 6 /ml), either as a mixed suspension or separated in transwell plates. Proliferative responses were determined in co-cultures with or without transwells. The proliferative responses are those of the CD25 − CD4 + effector cells in the lower wells of a transwell. ( C ) Different CD25- and 3G11-related T cell subpopulations from IL-10 −/− mice and their wild type control were purified by FACS sorting and were co-cultured with anti-CD3 stimulated CD25 − CD4 + effector T cells (both at 1×10 6 /ml). Columns refer to mean values and bars to SD. * represents the comparison of CD25 − CD4 + effector T cells only with other groups; @ represents the comparison of CD25 − 3G11 + T cells with other groups; and # represents the comparison of IL-10 −/− mice with wild type groups. *, p

    Article Snippet: After 72 hours of culture, stained cells were analyzed using FACSAria (Becton Dickinson).

    Techniques: Co-Culture Assay, Expressing, Mouse Assay, Staining, Cell Culture, Incubation, Purification, FACS

    Repression of activation-induced cytidine deaminase (AID) induction in germinal center B cells (GCBs) from Pten fl/fl Cγ1 Cre/+ mice. (A) Flow cytometry sorting of in vitro induced IgM-BCR expressing splenic B cells from Pten +/+ Cγ1 Cre/+ and Pten fl/fl Cγ1 Cre/+ mice. Pure splenic B cells from control and KO mice (three mice for each group) were stimulated with LPS plus interleukin-4 (IL-4) for 4 days before sorting. (B) The expression levels of PTEN, p-AKT, p-FoxO1/3a, and AID were determined in Pten +/+ Cγ1 Cre/+ and Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells (left). Reduced PTEN, AID expression and enhanced p-AKT and p-FoxO1/3a expression were observed in Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells. The in vitro induced IgM-BCR expressing B cells were isolated by the FACSAria III Cell Sorter as described in (A) . Statistical comparison of protein expression level was also shown in right. Ratio values of different proteins from Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells were normalized to that of the Pten +/+ Cγ1 Cre/+ IgM-BCR expressing B cells. Data were given from one representative of at least three independent experiments. * p

    Journal: Frontiers in Immunology

    Article Title: PTEN-Regulated AID Transcription in Germinal Center B Cells Is Essential for the Class-Switch Recombination and IgG Antibody Responses

    doi: 10.3389/fimmu.2018.00371

    Figure Lengend Snippet: Repression of activation-induced cytidine deaminase (AID) induction in germinal center B cells (GCBs) from Pten fl/fl Cγ1 Cre/+ mice. (A) Flow cytometry sorting of in vitro induced IgM-BCR expressing splenic B cells from Pten +/+ Cγ1 Cre/+ and Pten fl/fl Cγ1 Cre/+ mice. Pure splenic B cells from control and KO mice (three mice for each group) were stimulated with LPS plus interleukin-4 (IL-4) for 4 days before sorting. (B) The expression levels of PTEN, p-AKT, p-FoxO1/3a, and AID were determined in Pten +/+ Cγ1 Cre/+ and Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells (left). Reduced PTEN, AID expression and enhanced p-AKT and p-FoxO1/3a expression were observed in Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells. The in vitro induced IgM-BCR expressing B cells were isolated by the FACSAria III Cell Sorter as described in (A) . Statistical comparison of protein expression level was also shown in right. Ratio values of different proteins from Pten fl/fl Cγ1 Cre/+ IgM-BCR expressing B cells were normalized to that of the Pten +/+ Cγ1 Cre/+ IgM-BCR expressing B cells. Data were given from one representative of at least three independent experiments. * p

    Article Snippet: Pre-incubated IgM-BCR expressing B cells were sorted by using FACSAria III Cell Sorter (BD).

    Techniques: Activation Assay, Mouse Assay, Flow Cytometry, Cytometry, In Vitro, Expressing, Isolation

    Verification of Muse cells after FACSAria III separation. A. Percentage of Muse cells among MSCs. B. Percentage of Muse cells within the positive fraction. C. Percentage of non-Muse cells within the negative fraction.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: In vitro differentiation of human multilineage differentiating stress-enduring (Muse) cells into insulin producing cells

    doi: 10.1016/j.jgeb.2018.09.003

    Figure Lengend Snippet: Verification of Muse cells after FACSAria III separation. A. Percentage of Muse cells among MSCs. B. Percentage of Muse cells within the positive fraction. C. Percentage of non-Muse cells within the negative fraction.

    Article Snippet: SSEA-3 positive fraction (Muse cells) was isolated by SORP FACSAria III (BDbiosciences).

    Techniques:

    Adjuvanticity of FlgE to Soluble Antigen in Mice. ( A–C ) Naïve OVA-specific T cells were purified from the spleens and inguinal lymph nodes of CD45.1 × OTII F1 mice by sorting using various antibodies to surface markers, labeled with eFluor450 and transferred into female C57BL/6 mice at 1 × 10 6 cells/mouse via tail vein injection. Twenty-four hours later, the recipients were treated or immunized via subcutaneous injection at the base of the tail with one of eight compositions, namely 1 μg OVA, 50 μg CpG-1826, 50 μg FlgE, 50 μg FlgEM each alone, or OVA plus the three stimulants individually. Control groups received 100 μL PBS. Three days later, the mice were sacrificed, and the draining inguinal lymph nodes were isolated and photographed ( A ). Single-cell suspension were made for staining with CD45.1 plus CD4 and then run on a BD FACSAria II and analyzed for eFluor450 intensity to quantify the proliferation of OVA-specific T cells ( B,C ). ( D ) To measure the humoral response, WT C57BL/6 mice were immunized with above 8 compositions except for increasing OVA doses to 100 μg. Two weeks later, the mice were sacrificed for serum harvest, and anti-OVA titers were measured using an ELISA as described in Materials and Methods. Data are representatives of two independent experiments that showed similar results. n = 3 mice for panel A–C, and n = 4 mice for panel D. *P

    Journal: Scientific Reports

    Article Title: Flagellar Hooks and Hook Protein FlgE Participate in Host Microbe Interactions at Immunological Level

    doi: 10.1038/s41598-017-01619-1

    Figure Lengend Snippet: Adjuvanticity of FlgE to Soluble Antigen in Mice. ( A–C ) Naïve OVA-specific T cells were purified from the spleens and inguinal lymph nodes of CD45.1 × OTII F1 mice by sorting using various antibodies to surface markers, labeled with eFluor450 and transferred into female C57BL/6 mice at 1 × 10 6 cells/mouse via tail vein injection. Twenty-four hours later, the recipients were treated or immunized via subcutaneous injection at the base of the tail with one of eight compositions, namely 1 μg OVA, 50 μg CpG-1826, 50 μg FlgE, 50 μg FlgEM each alone, or OVA plus the three stimulants individually. Control groups received 100 μL PBS. Three days later, the mice were sacrificed, and the draining inguinal lymph nodes were isolated and photographed ( A ). Single-cell suspension were made for staining with CD45.1 plus CD4 and then run on a BD FACSAria II and analyzed for eFluor450 intensity to quantify the proliferation of OVA-specific T cells ( B,C ). ( D ) To measure the humoral response, WT C57BL/6 mice were immunized with above 8 compositions except for increasing OVA doses to 100 μg. Two weeks later, the mice were sacrificed for serum harvest, and anti-OVA titers were measured using an ELISA as described in Materials and Methods. Data are representatives of two independent experiments that showed similar results. n = 3 mice for panel A–C, and n = 4 mice for panel D. *P

    Article Snippet: Cells were run on a BD FACSAria II and analyzed for eFluor450 intensity to reflect proliferation.

    Techniques: Mouse Assay, Purification, Labeling, Injection, Isolation, Staining, Enzyme-linked Immunosorbent Assay